ISSpu16
- Family IS5
- Group IS903
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009438 | ND | Shewanella putrefaciens | Shewanella putrefaciens CN-32 Shewanella sp. ANA-3 |
DNA section
IS Length : 1055 bp
Ends
IR Length : 20/21
IRL : GGCGTTGTTGCCTAAATGAATGAACCTTCCGTCTCAGATGCGATCAGATC
IRR : GGCGTTGTTGCCTAAATCAATCTTAGGTATGCCTTTTCCTAAAAGGGTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTTATTTTG | CGTGCTCAAC | AGAAAATAAC | 10 |
| CCTTCTGCGG | CTTATCAGAT | 0 |
DNA sequence
GGCGTTGTTGCCTAAATGAATGAACCTTCCGTCTCAGATGCGATCAGATCCTGTGAATACCACGCAGGAACCGCTATCAGATGGGCAAATCTAAATCTCG
CATTACCAATTGGCCGGAATATAACAAGGCGTTGACCAACCGAGGCTCACTGACTTTTTGGATAGACGAGCAGGCGCTCAACCTCTGGTGTCACACTGAA
CACCATGGTGGTCGAGGTCGGGGATTTCAATACAGTGACACGGCGATAGAAACCGCCTTAATGCTCAAAGGTTTGTTTAATCTCCCCTTGCGAGCACTAG
AAGGTTTTCTCAACTCAGTGTTTAGTTTGATGAGTCTGCAATTAACTTCACCTGGTTACAGCTGCATCAGCAAGCGGGCAAAGACAGTCAAAGTTAACTA
CCGCCCCCCCTTGCCGAGGCGCGATTGCTCACTTAGTTGTCGATGCCACAGGACTGAAAGTATATGGCGAAGGTGAATGGAAAATGCGTAAACATGGTAA
GGAAAAACGCCGAACTTGGCGTAAGTTGCACCTTGCGATAGATGCGGCCACACATGAAGTCATCGCAGCCGAAGTCAGTTTAGAGAGTGTGGCAGATAGT
GAGGTTCTCCCAACGTTATTAAACCCACTAAGGCGCAAGGTAAAACAAGTGTCAGCTGACGGTGCTTATGACACCAAAAAATGTCATAAGCTGCTGAAAC
GCAGAGGGTGTAAACCTACGATACCGCCGCGAAAAAATGCAGGTTATTGGGAAGAAGGTCATCCCAGAAACGAGGCCGTCAGCGCTCTGAAAAGTGATGA
ACTCAAACAATGGAAACAAGAGAATGACTATCACCAACGCTCCCTGTCAGAAACAGCTATGTATCGTTACAAGCAACTGATTAGCCCGAAATTGAGCCTG
CGGGACTACAACGGCCAAGTCGGCGAGGCACTGGCTGGTGTGAAAGTGATGAACAAAGTCATAGGACTGGGTATGCCTATTCGCCAGAGGGTCAATTAAT
ACCAGGAACCCTTTTAGGAAAAGGCATACCTAAGATTGATTTAGGCAACAACGCC
CATTACCAATTGGCCGGAATATAACAAGGCGTTGACCAACCGAGGCTCACTGACTTTTTGGATAGACGAGCAGGCGCTCAACCTCTGGTGTCACACTGAA
CACCATGGTGGTCGAGGTCGGGGATTTCAATACAGTGACACGGCGATAGAAACCGCCTTAATGCTCAAAGGTTTGTTTAATCTCCCCTTGCGAGCACTAG
AAGGTTTTCTCAACTCAGTGTTTAGTTTGATGAGTCTGCAATTAACTTCACCTGGTTACAGCTGCATCAGCAAGCGGGCAAAGACAGTCAAAGTTAACTA
CCGCCCCCCCTTGCCGAGGCGCGATTGCTCACTTAGTTGTCGATGCCACAGGACTGAAAGTATATGGCGAAGGTGAATGGAAAATGCGTAAACATGGTAA
GGAAAAACGCCGAACTTGGCGTAAGTTGCACCTTGCGATAGATGCGGCCACACATGAAGTCATCGCAGCCGAAGTCAGTTTAGAGAGTGTGGCAGATAGT
GAGGTTCTCCCAACGTTATTAAACCCACTAAGGCGCAAGGTAAAACAAGTGTCAGCTGACGGTGCTTATGACACCAAAAAATGTCATAAGCTGCTGAAAC
GCAGAGGGTGTAAACCTACGATACCGCCGCGAAAAAATGCAGGTTATTGGGAAGAAGGTCATCCCAGAAACGAGGCCGTCAGCGCTCTGAAAAGTGATGA
ACTCAAACAATGGAAACAAGAGAATGACTATCACCAACGCTCCCTGTCAGAAACAGCTATGTATCGTTACAAGCAACTGATTAGCCCGAAATTGAGCCTG
CGGGACTACAACGGCCAAGTCGGCGAGGCACTGGCTGGTGTGAAAGTGATGAACAAAGTCATAGGACTGGGTATGCCTATTCGCCAGAGGGTCAATTAAT
ACCAGGAACCCTTTTAGGAAAAGGCATACCTAAGATTGATTTAGGCAACAACGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 919 bp | 305 aa | 81 | 999 | + | No |
Chemistry : DDE
ORF sequence :
MGKSKSRITNWPEYNKALTNRGSLTFWIDEQALNLWCHTEHHGGRGRGFQYSDTAIETALMLKGLFNLPLRALEGFLNSVFSLMSLQLTSPGYSCISKRA
KTVKVNYRPPCRGAIAHLVVDATGLKVYGEGEWKMRKHGKEKRRTWRKLHLAIDAATHEVIAAEVSLESVADSEVLPTLLNPLRRKVKQVSADGAYDTKK
CHKLLKRRGCKPTIPPRKNAGYWEEGHPRNEAVSALKSDELKQWKQENDYHQRSLSETAMYRYKQLISPKLSLRDYNGQVGEALAGVKVMNKVIGLGMPI
RQRVN
KTVKVNYRPPCRGAIAHLVVDATGLKVYGEGEWKMRKHGKEKRRTWRKLHLAIDAATHEVIAAEVSLESVADSEVLPTLLNPLRRKVKQVSADGAYDTKK
CHKLLKRRGCKPTIPPRKNAGYWEEGHPRNEAVSALKSDELKQWKQENDYHQRSLSETAMYRYKQLISPKLSLRDYNGQVGEALAGVKVMNKVIGLGMPI
RQRVN
Blast result :
Comments
There is one copy of ISSpu16 in the Shewanella putrefaciens CN-32 genome.
The ISSpu16 transposase has 68% identity with the ISVa1 transposase.
The transposase was reconstructed in silico by deletion of one C on position 408 ( this base generate a potential frameshift, due to a sequence error or to a ponctual mutation).
The ISSpu16 transposase has 68% identity with the ISVa1 transposase.
The transposase was reconstructed in silico by deletion of one C on position 408 ( this base generate a potential frameshift, due to a sequence error or to a ponctual mutation).
References
1] Romine, M.F. (2008) Direct submission.