ISSlo2
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009092 | ND | Shewanella loihica | Shewanella loihica PV-4 |
DNA section
IS Length : 1257 bp
Ends
IR Length : 21/26
IRL : TGTAGTGGCATAGTAAATTTGGCCACCTGAATAGAGGAAGTGATAGACTG
IRR : TGTAGTGGTCAACTAAAATTGGCCACGGTTTTGTATTCAAGCCAGTATCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTTAGAAGTG | CTATAAGCAA | 0 |
DNA sequence
TGTAGTGGCATAGTAAATTTGGCCACCTGAATAGAGGAAGTGATAGACTGACTTCCAGAAAAAAGGTGGTAGAAATGAAGAACACAAGACCGACATTCAG
TGCAGAGTTCAAGTTAGAAGCTGCACAACTGGTAACGAAACAGGGCTACAGCGTTACTGAGGCAGCAAGCGCGATGGGCGTAAGTAATTCAGCCATGCGA
AAGTGGGTTAACCAGCTTCGACAGGAGCAGCAAGGCGTATCACCCAAAGGCGCACCGCTCACCCCCGAACAACAGCGGATCCGCGAGCTGGAGAAGAAAA
TCCGCCGCATTGAAGAAGAGAACACCATTTTGAAAAAGGCTACGGCTCTCTTGATGTCAGACTCACTGAACAGTTCTCGTTAGTCGAGAAACTCAGGAAG
AGCCATAGCGTAAAGCGGATTTGTTCAGTATTTGGTATTCACCGCAGCAGTTACAAGTATTGGCGAGGCAGACCTACCACCATTGACTCGAAACAGGTAA
AACTGCACAGCTTGGTGCGGGAGGCTTATGAAGCCAGCAATGGGTCAGCGGGTGCCAGAACCCTGACTGATATGGTAACGACTGCTGGCGTGAGGTTGAC
GCGCTATCGTGCCACAGGGCTGATGAAGAAGCTGGGGCTAGTGAGCTGCCAGGTGCCGCAACATCGCTACAAAAAGGCAGACAAGGAGCATGTCGCCATC
CCTAACCTCCTTGACCGCCAGTTTGCTGTGACGGCTCCCAATCAAGTGTGGTGTGGCGATGTGACCTATGTGTGGACAGGCAAACGATGGGCTTACCTTG
CCGTTGTTCTGGACTTGTTTGCACGGAAACCGGTGGGGTGGGCGATGTCATTTTCGCCCGATAGTGCGCTAACAAGCAAGGCACTAGCCATGGCCTTTGA
GGCGCGAGGAAGGCCGAAAGACCTAATGTTCCATAGTGACCAAGGAAGCCACTATACCAGCCGCCGCTTCCGCCAACAGCTCTGGCGTTATCAGATTAAG
CAGAGCATGAGTCGACGAGGAAACTGCTGGGACAACAGCCCGATGGAGCGCTTCTTTAGAAGCTTAAAAACAGAATGGATACCGATGACCGGCTATCGGG
ATATCGGTGAGGCTAAGAGTGAAATTTCGCGATATGTGACGGGGTATTACAGTCAGCTAAGACCACATCAATATAACGGTGGGCTGACACCCAATGAGTC
AGAGCGAAGATACTGGCTTGAATACAAAACCGTGGCCAATTTTAGTTGACCACTACA
TGCAGAGTTCAAGTTAGAAGCTGCACAACTGGTAACGAAACAGGGCTACAGCGTTACTGAGGCAGCAAGCGCGATGGGCGTAAGTAATTCAGCCATGCGA
AAGTGGGTTAACCAGCTTCGACAGGAGCAGCAAGGCGTATCACCCAAAGGCGCACCGCTCACCCCCGAACAACAGCGGATCCGCGAGCTGGAGAAGAAAA
TCCGCCGCATTGAAGAAGAGAACACCATTTTGAAAAAGGCTACGGCTCTCTTGATGTCAGACTCACTGAACAGTTCTCGTTAGTCGAGAAACTCAGGAAG
AGCCATAGCGTAAAGCGGATTTGTTCAGTATTTGGTATTCACCGCAGCAGTTACAAGTATTGGCGAGGCAGACCTACCACCATTGACTCGAAACAGGTAA
AACTGCACAGCTTGGTGCGGGAGGCTTATGAAGCCAGCAATGGGTCAGCGGGTGCCAGAACCCTGACTGATATGGTAACGACTGCTGGCGTGAGGTTGAC
GCGCTATCGTGCCACAGGGCTGATGAAGAAGCTGGGGCTAGTGAGCTGCCAGGTGCCGCAACATCGCTACAAAAAGGCAGACAAGGAGCATGTCGCCATC
CCTAACCTCCTTGACCGCCAGTTTGCTGTGACGGCTCCCAATCAAGTGTGGTGTGGCGATGTGACCTATGTGTGGACAGGCAAACGATGGGCTTACCTTG
CCGTTGTTCTGGACTTGTTTGCACGGAAACCGGTGGGGTGGGCGATGTCATTTTCGCCCGATAGTGCGCTAACAAGCAAGGCACTAGCCATGGCCTTTGA
GGCGCGAGGAAGGCCGAAAGACCTAATGTTCCATAGTGACCAAGGAAGCCACTATACCAGCCGCCGCTTCCGCCAACAGCTCTGGCGTTATCAGATTAAG
CAGAGCATGAGTCGACGAGGAAACTGCTGGGACAACAGCCCGATGGAGCGCTTCTTTAGAAGCTTAAAAACAGAATGGATACCGATGACCGGCTATCGGG
ATATCGGTGAGGCTAAGAGTGAAATTTCGCGATATGTGACGGGGTATTACAGTCAGCTAAGACCACATCAATATAACGGTGGGCTGACACCCAATGAGTC
AGAGCGAAGATACTGGCTTGAATACAAAACCGTGGCCAATTTTAGTTGACCACTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 309 bp | 102 aa | 75 | 383 | + | No |
Description : First part of the transposase
ORF sequence :
MKNTRPTFSAEFKLEAAQLVTKQGYSVTEAASAMGVSNSAMRKWVNQLRQEQQGVSPKGAPLTPEQQRIRELEKKIRRIEEENTILKKATALLMSDSLNS
SR
SR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 936 bp | 311 aa | 314 | 1249 | + | No |
Description : Second part of the transposase
ORF sequence :
RREHHFEKGYGSLDVRLTEQFSLVEKLRKSHSVKRICSVFGIHRSSYKYWRGRPTTIDSKQVKLHSLVREAYEASNGSAGARTLTDMVTTAGVRLTRYRA
TGLMKKLGLVSCQVPQHRYKKADKEHVAIPNLLDRQFAVTAPNQVWCGDVTYVWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSALTSKALAMAFEARGR
PKDLMFHSDQGSHYTSRRFRQQLWRYQIKQSMSRRGNCWDNSPMERFFRSLKTEWIPMTGYRDIGEAKSEISRYVTGYYSQLRPHQYNGGLTPNESERRY
WLEYKTVANFS
TGLMKKLGLVSCQVPQHRYKKADKEHVAIPNLLDRQFAVTAPNQVWCGDVTYVWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSALTSKALAMAFEARGR
PKDLMFHSDQGSHYTSRRFRQQLWRYQIKQSMSRRGNCWDNSPMERFFRSLKTEWIPMTGYRDIGEAKSEISRYVTGYYSQLRPHQYNGGLTPNESERRY
WLEYKTVANFS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1175 bp | 391 aa | 75 | 1249 | + | Yes |
Chemistry : DDE
ORF sequence :
MKNTRPTFSAEFKLEAAQLVTKQGYSVTEAASAMGVSNSAMRKWVNQLRQEQQGVSPKGAPLTPEQQRIRELEKKIRRIEEENTILKKGYGSLDVRLTEQ
FSLVEKLRKSHSVKRICSVFGIHRSSYKYWRGRPTTIDSKQVKLHSLVREAYEASNGSAGARTLTDMVTTAGVRLTRYRATGLMKKLGLVSCQVPQHRYK
KADKEHVAIPNLLDRQFAVTAPNQVWCGDVTYVWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSALTSKALAMAFEARGRPKDLMFHSDQGSHYTSRRFR
QQLWRYQIKQSMSRRGNCWDNSPMERFFRSLKTEWIPMTGYRDIGEAKSEISRYVTGYYSQLRPHQYNGGLTPNESERRYWLEYKTVANFS
FSLVEKLRKSHSVKRICSVFGIHRSSYKYWRGRPTTIDSKQVKLHSLVREAYEASNGSAGARTLTDMVTTAGVRLTRYRATGLMKKLGLVSCQVPQHRYK
KADKEHVAIPNLLDRQFAVTAPNQVWCGDVTYVWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSALTSKALAMAFEARGRPKDLMFHSDQGSHYTSRRFR
QQLWRYQIKQSMSRRGNCWDNSPMERFFRSLKTEWIPMTGYRDIGEAKSEISRYVTGYYSQLRPHQYNGGLTPNESERRYWLEYKTVANFS
Blast result :
Comments
There is one copy of ISSlo2 encoded in the Shewanella loihica PV-4 genome.
The ISSlo2 transposase has 71% identity to ISAlg.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The ISSlo2 transposase has 71% identity to ISAlg.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Romine, M.F. (2008) Direct submission.