ISCysp4
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011884 | ND | Cyanothece sp. | Cyanothece sp. PCC 7425 plasmid pP742501 Cyanothece sp. PCC 7425 Cyanothece sp. PCC 7425 plasmid pP742502 |
DNA section
IS Length : 1585 bp
Ends
IR Length : 14/17
IRL : GTAACTGTTCAAGGGGTTGACAAAATTTAATTGAGAAAACCAGGGGTAGC
IRR : GTAACCGTTCAGAGGGTGCGATCTTGAATTGGTAACAAAGAGGGTGCTGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGTTGCCGTT | GGTTTGTC | TTTGGCCGGC | 8 |
| GATTTAACAA | CCTGACTG | ATTCAAATAT | 8 |
| GTGTGCATAA | GAACGGAC | AGATTGTGAT | 8 |
| TCATCCGGTA | GAGAATTG | ACTACCTCAA | 8 |
| AGGTTGTACT | GGCTCAAC | ACGATTGATT | 8 |
| CGGTTCTTAA | GACCTTTT | TATCGTCAAG | 8 |
| GGTATTCCCT | GGTGATAG | CTCTCTGTAA | 8 |
| GCTTCCAACT | CTTGCAAC | GATGGTTAGA | 8 |
| ATCAAAAATT | TTTTTTGC | TTCTCCTATC | 8 |
| GCCAAGGTGA | TAAAACTG | TGCCGGGTCT | 8 |
| TTTTTCATCA | GTTCAGTT | TTTGGCGTGC | 8 |
| GAAATTGCCT | TGGAAATC | TTTTGCCCAC | 8 |
DNA sequence
GTAACTGTTCAAGGGGTTGACAAAATTTAATTGAGAAAACCAGGGGTAGCTGTTAGGGTAGCAGGATGATGGAACCCCTTCCCGTGTCGATTGAGCAGAT
TAGCGATTCAGAGTGGGTCGAAACACCGGAGAGTGTGAAGCGTTATCTTTTGGGACGCATCGAGCAATTAGAACGACAATACGAGGAGTTGAAAGCGGAA
AACGCAATGCTGCGAGAGCAACTTAGGCGCAATAGCCAAAACTCGTCAAAACCCCCGTCCCAGGATCAGGGCAACAGGTTTAAGCCCAAGGCAAAGCAGG
AGAAGGAGAAGAAGAAACCACGAGGTGGGCAATTGGGGCATGAGGGTCATGAAGGGAAATTTTATCCTTTAGAAGCGTGTGAGACGGTTACGGAACACTA
CCCCGAACGCTGCATCAACTGTGGAGGGAGGTTGCAGGGCAAAGACCCAGAACCGTATCGGGTTCAACAGGTCGAGATTCCTCCAGTAGTGCCGGTCGTG
AGGGAACATCGGTTTCATGCCTTAAAGTGTGCCTGTTGTGGTGTGAGAACCAGAGCTTGGGATGAAGTCATCATCAATGGATGTCGGTATGGGGAACGGA
TTGTCTCGACAGTGGGTCTCTTGAGTGGGCAGTATCGGCAGTCGCATCGGATGGTGCAAGGGTTGCTCGACCAGTTGTTTGGGGTTGAGTTATCGACCGG
CAGCATCAACCAACTGCGGCAGGAGAGTAGCACCTCGGTCGCCAGCGCAGTGGAGGAAGCTCACCACTATGTGCAACAGCAAGAGCAAGTGAATATGGAC
GAAACGAGCTTTGCTCAAGGCAACAACGATGGCAAGAATACTGAGGGACGCAAGGGTTGGTTGTGGGTGCTAGTGACCCCATGGGTGAGTTACTTTGCGG
TGTGTTTGAGCCGTTCTAGTCAGGTCTGCCAGCAGTTGTTGGGTGAGACGTTTACAGGCATCGTTGGCAGCGACCGCTACAGTGCCTACCAGCATTTGAG
CCTTGAGCACCGACAGGTGTGTTGGGCGCATCTCAAAAGGGATTTGACTCAAATCGCTGAACGCAAAGGTGTGGCGGGAGAACTGGGTCAAGCCTTACTG
GAACAACAGCATTTATTGTTTGAACACTGGTATCGGGTGCGCGATGGAACCCTCAGTCGAAGTGAGTTTTCTCAAACAGTGCAACCGATTCGAGAACGAT
TTAAGCAATTGCTCACAGAAGGGGCAGGGTATGAGATTGCACCCAAAGAGAAAACTCCCTTGGCGAAAACCGTTCGCACTTGCCAGCAATTGTTGAAAAT
CGAACCTGCGCTCTGGACCTTTGTGACGACTGAGGGCATCGAACCCACCAACAATGCGGCGGAGCGGGCACTTCGTCCAGCGGTACTATGGCGCAAAAAT
AGTTTCGGTTCCCAGAGTGAGGCAGGCAGTTTGTTTGTCTCTCGAATGCTCACGGTCACCACTTCTTTGCGGGCCCAAAATCGTTCGGTATTGGAGTATT
TAGTGCAAGCGTGCCGCGCTTCCAGGCAGGGTCTACCAGCACCCTCTTTGTTACCAATTCAAGATCGCACCCTCTGAACGGTTAC
TAGCGATTCAGAGTGGGTCGAAACACCGGAGAGTGTGAAGCGTTATCTTTTGGGACGCATCGAGCAATTAGAACGACAATACGAGGAGTTGAAAGCGGAA
AACGCAATGCTGCGAGAGCAACTTAGGCGCAATAGCCAAAACTCGTCAAAACCCCCGTCCCAGGATCAGGGCAACAGGTTTAAGCCCAAGGCAAAGCAGG
AGAAGGAGAAGAAGAAACCACGAGGTGGGCAATTGGGGCATGAGGGTCATGAAGGGAAATTTTATCCTTTAGAAGCGTGTGAGACGGTTACGGAACACTA
CCCCGAACGCTGCATCAACTGTGGAGGGAGGTTGCAGGGCAAAGACCCAGAACCGTATCGGGTTCAACAGGTCGAGATTCCTCCAGTAGTGCCGGTCGTG
AGGGAACATCGGTTTCATGCCTTAAAGTGTGCCTGTTGTGGTGTGAGAACCAGAGCTTGGGATGAAGTCATCATCAATGGATGTCGGTATGGGGAACGGA
TTGTCTCGACAGTGGGTCTCTTGAGTGGGCAGTATCGGCAGTCGCATCGGATGGTGCAAGGGTTGCTCGACCAGTTGTTTGGGGTTGAGTTATCGACCGG
CAGCATCAACCAACTGCGGCAGGAGAGTAGCACCTCGGTCGCCAGCGCAGTGGAGGAAGCTCACCACTATGTGCAACAGCAAGAGCAAGTGAATATGGAC
GAAACGAGCTTTGCTCAAGGCAACAACGATGGCAAGAATACTGAGGGACGCAAGGGTTGGTTGTGGGTGCTAGTGACCCCATGGGTGAGTTACTTTGCGG
TGTGTTTGAGCCGTTCTAGTCAGGTCTGCCAGCAGTTGTTGGGTGAGACGTTTACAGGCATCGTTGGCAGCGACCGCTACAGTGCCTACCAGCATTTGAG
CCTTGAGCACCGACAGGTGTGTTGGGCGCATCTCAAAAGGGATTTGACTCAAATCGCTGAACGCAAAGGTGTGGCGGGAGAACTGGGTCAAGCCTTACTG
GAACAACAGCATTTATTGTTTGAACACTGGTATCGGGTGCGCGATGGAACCCTCAGTCGAAGTGAGTTTTCTCAAACAGTGCAACCGATTCGAGAACGAT
TTAAGCAATTGCTCACAGAAGGGGCAGGGTATGAGATTGCACCCAAAGAGAAAACTCCCTTGGCGAAAACCGTTCGCACTTGCCAGCAATTGTTGAAAAT
CGAACCTGCGCTCTGGACCTTTGTGACGACTGAGGGCATCGAACCCACCAACAATGCGGCGGAGCGGGCACTTCGTCCAGCGGTACTATGGCGCAAAAAT
AGTTTCGGTTCCCAGAGTGAGGCAGGCAGTTTGTTTGTCTCTCGAATGCTCACGGTCACCACTTCTTTGCGGGCCCAAAATCGTTCGGTATTGGAGTATT
TAGTGCAAGCGTGCCGCGCTTCCAGGCAGGGTCTACCAGCACCCTCTTTGTTACCAATTCAAGATCGCACCCTCTGAACGGTTAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1512 bp | 503 aa | 66 | 1577 | + | No |
Chemistry : DDE
ORF sequence :
MMEPLPVSIEQISDSEWVETPESVKRYLLGRIEQLERQYEELKAENAMLREQLRRNSQNSSKPPSQDQGNRFKPKAKQEKEKKKPRGGQLGHEGHEGKFY
PLEACETVTEHYPERCINCGGRLQGKDPEPYRVQQVEIPPVVPVVREHRFHALKCACCGVRTRAWDEVIINGCRYGERIVSTVGLLSGQYRQSHRMVQGL
LDQLFGVELSTGSINQLRQESSTSVASAVEEAHHYVQQQEQVNMDETSFAQGNNDGKNTEGRKGWLWVLVTPWVSYFAVCLSRSSQVCQQLLGETFTGIV
GSDRYSAYQHLSLEHRQVCWAHLKRDLTQIAERKGVAGELGQALLEQQHLLFEHWYRVRDGTLSRSEFSQTVQPIRERFKQLLTEGAGYEIAPKEKTPLA
KTVRTCQQLLKIEPALWTFVTTEGIEPTNNAAERALRPAVLWRKNSFGSQSEAGSLFVSRMLTVTTSLRAQNRSVLEYLVQACRASRQGLPAPSLLPIQD
RTL
PLEACETVTEHYPERCINCGGRLQGKDPEPYRVQQVEIPPVVPVVREHRFHALKCACCGVRTRAWDEVIINGCRYGERIVSTVGLLSGQYRQSHRMVQGL
LDQLFGVELSTGSINQLRQESSTSVASAVEEAHHYVQQQEQVNMDETSFAQGNNDGKNTEGRKGWLWVLVTPWVSYFAVCLSRSSQVCQQLLGETFTGIV
GSDRYSAYQHLSLEHRQVCWAHLKRDLTQIAERKGVAGELGQALLEQQHLLFEHWYRVRDGTLSRSEFSQTVQPIRERFKQLLTEGAGYEIAPKEKTPLA
KTVRTCQQLLKIEPALWTFVTTEGIEPTNNAAERALRPAVLWRKNSFGSQSEAGSLFVSRMLTVTTSLRAQNRSVLEYLVQACRASRQGLPAPSLLPIQD
RTL
Blast result :
Comments
ISCysp4 is 57% aa similar to ISStau3.
References
1] ISfinder annotation (2008)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Meineke,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Liberton,M., Stoeckel,J., Banerjee,A., Singh,A., Page,L., Sato,H., Zhao,L., Sherman,L., Pakrasi,H. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Meineke,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Liberton,M., Stoeckel,J., Banerjee,A., Singh,A., Page,L., Sato,H., Zhao,L., Sherman,L., Pakrasi,H. and Richardson,P. (2009) Direct submission GenBank.