ISVme1
- Family IS66
- Group
Isoform Synonym(s) ISVme
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF525312 | ND | Vibrio metschnikovii | Vibrio metschnikovii CIP A267 |
DNA section
IS Length : 2372 bp
Ends
IR Length : 16/21
IRL : GTAAGCGTTTATTTAAACCCAACCTACCAATAACTCAGCATTCTAGGCAT
IRR : GTAAGCGCCTAGTTAACCAGTGAGACCTTATTCAGCCTCGCTGATTTCTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GTAAGCGTTTATTTAAACCCAACCTACCAATAACTCAGCATTCTAGGCATAGTGATTTCGCAACCAAGGAGTCACTATGAACCAATCGAACAAAACTCAA
TTCTGGGCAGAAATCATTGAGCAACAGAGCCAAAGTGAGTTATCCATTGTCGCGTTTTGCAAGGCAAACCAACTGACGGTAAATACCTTTTACTACTGGC
GCAAAAAGCTTTATGGGGGCGAACAGCAACAAGTGGTGCATCCTGTCCTGATAGAAGATAGCCCGAAAAGCATAGGGGAACGCCAGTCGGTTATGCTTTC
ACTACCAACAGGAATGACCGTTGAATTTCCCTCTAACATGCCAGCACACCAAATCCAAAACTGGTTACAGGCTCTGACGTGTTAATACCGAACTTGGGTA
TTTACCTGGTCTCGGGCATAACTGACATGCGCAAGTCTATTGATGGCTTGTCATTGATTGTGGCTGATGTGTCGGAAATGGATCCGTTCAGTGAAGCCTG
GTTTATCTTCTGCAACCGCGGCCGAGACAAAATTAAGATCTTGTTCTGGGATACTAATGGTTTTTGGCTTTATTACCGCCGACTGGAAAAAGGTCGTTTT
AACTGGCCTAAACCCAATGCATCTGGTCATGTCGCTATCTCCAGACAGCAACTTCAATGGTTACTCTCTGGGCTTAATCTGGAACACCCTAAAGCTCATC
AGCCCTTATATGGCCTTGAAGTGTGAGCCAATACCATGATCGTGATAAAGGACTTGAACGATCCTTTTACCCTGTCAGACTAACAGCATAATCCTGACCA
AGAGCAGCCTGACCGATGAATCACCTTCCTGACGATGTAGAACAACTGAAAGCGATACTGTTAGCACTTCAGGAAGAAAACAAAGCGAAGGACAGGCAAC
TACAGGCCCAGCAAGAAGAAATGGCTGAGCTTAAAACACGGGTGGAACTCCTGCTTGAGCAACTCAACCTCAGTAAATCCAAACGGTTCTCTTCCCAGAG
TGAAAAGGTGGTTAAAGGTACCTTCAATGAAGCCGAGCAGCAAAATGCACTGCACAAACCAGAGCCAGCTCATCATAAAAAAGGACGTAAACCGCTACCT
GAAGAGCTGGAGCGAGAAGTTCAGGTTCACACTCTGAATGCCCCACACTGCAAATGTTGTGGGCACGAATTACATAAATGTAGGGTGGAAACCTCTGAAG
AGCTGAAGATCATCCCTCAGAAAATCAGTGTGATCCGCCATGAACGAACCAAATATGCCTGCCGTCAGTGTGAACACACCGAAACCAGCAGCAAGATCGT
CACAGCCCCCAAACCCGCCAGCATGATCCCGAAAAGTATCGGTTGTCCAGAAACCTTCGCCGCCATCGTTACTGCCAAATACGTTGATGCGCTACCGCTA
TACCGTCAGGTTGATATTCTTAAGCGAGGCGACATCGATATTAGCCGAGGTACGCTGGCCAACTGGTGTGTTCAGCTAGGGAACAACGTTGACGTCATTA
TCGACCAGATGAAAGCACGCTTGTTATCTGAAAGCTTAATCTGCGCAGACGAAACCACGGTACAGGTACTGTCAGAGCCAGGGAAAACAGCGCAAAGCAG
GTCCTATATGTGGGTCTATCGCAGTGGCGAGTTCCATAAGTCACCTGTGGTTATCTATGATTATCAGCCAGGGCGCGGGGCAAACTGCCTCAACACCTTC
CTTGGTGATTACTCTGGTTATCTGCTGTCGGATGGGTATCCCGCATATGACACTCTTAAAGATGTCACGCAAGCGGGTTGCATGGCCCATGCAAGACGTA
AGTTCACCGATGCTCAAAAAGCTCAACCGACCAAGAAAGTTGGCCGTGTAGAAAAAGCGCTGAACTATATCGGGAAGCTTTACGGTATAGAACGGGAGAC
TAGAAAACTAAGCCCAGAAAAGCGGCGACAGATAAGGCAACAAAAAGCCAAGCCTATACTCGATGAGTTCCATCAATGGTTGGTCGGAATTAAGGAAACA
CAACTACCGAAAGGTAAGCTCGGGATCGCCATCAACTATGCGATAAACCAGTGGCCAAAACTTCTTACGTATCTTGAAGACGGTCATATCAGCATCGATA
ATAACGTGACTGAGAGAGATGTGCGTCCGTTTACCACGGGGCGCAAAAATTGGATGTTCTCAACTTCAGTAGACGGAGCAAAGGCCAGCGCAAACCTCTA
TAGCCTGGTCATGACTTGCCGTGCCAACGACATCAATCCCTACTTCTACTTCGTGCACCTGTTCAGGGAGTTACCGAAGCGTTCACCAAGCGATGATTTA
ACTGATCTTATGCCATGGAATGTAGAAATCAGCGAGGCTGAATAAGGTCTCACTGGTTAACTAGGCGCTTAC
TTCTGGGCAGAAATCATTGAGCAACAGAGCCAAAGTGAGTTATCCATTGTCGCGTTTTGCAAGGCAAACCAACTGACGGTAAATACCTTTTACTACTGGC
GCAAAAAGCTTTATGGGGGCGAACAGCAACAAGTGGTGCATCCTGTCCTGATAGAAGATAGCCCGAAAAGCATAGGGGAACGCCAGTCGGTTATGCTTTC
ACTACCAACAGGAATGACCGTTGAATTTCCCTCTAACATGCCAGCACACCAAATCCAAAACTGGTTACAGGCTCTGACGTGTTAATACCGAACTTGGGTA
TTTACCTGGTCTCGGGCATAACTGACATGCGCAAGTCTATTGATGGCTTGTCATTGATTGTGGCTGATGTGTCGGAAATGGATCCGTTCAGTGAAGCCTG
GTTTATCTTCTGCAACCGCGGCCGAGACAAAATTAAGATCTTGTTCTGGGATACTAATGGTTTTTGGCTTTATTACCGCCGACTGGAAAAAGGTCGTTTT
AACTGGCCTAAACCCAATGCATCTGGTCATGTCGCTATCTCCAGACAGCAACTTCAATGGTTACTCTCTGGGCTTAATCTGGAACACCCTAAAGCTCATC
AGCCCTTATATGGCCTTGAAGTGTGAGCCAATACCATGATCGTGATAAAGGACTTGAACGATCCTTTTACCCTGTCAGACTAACAGCATAATCCTGACCA
AGAGCAGCCTGACCGATGAATCACCTTCCTGACGATGTAGAACAACTGAAAGCGATACTGTTAGCACTTCAGGAAGAAAACAAAGCGAAGGACAGGCAAC
TACAGGCCCAGCAAGAAGAAATGGCTGAGCTTAAAACACGGGTGGAACTCCTGCTTGAGCAACTCAACCTCAGTAAATCCAAACGGTTCTCTTCCCAGAG
TGAAAAGGTGGTTAAAGGTACCTTCAATGAAGCCGAGCAGCAAAATGCACTGCACAAACCAGAGCCAGCTCATCATAAAAAAGGACGTAAACCGCTACCT
GAAGAGCTGGAGCGAGAAGTTCAGGTTCACACTCTGAATGCCCCACACTGCAAATGTTGTGGGCACGAATTACATAAATGTAGGGTGGAAACCTCTGAAG
AGCTGAAGATCATCCCTCAGAAAATCAGTGTGATCCGCCATGAACGAACCAAATATGCCTGCCGTCAGTGTGAACACACCGAAACCAGCAGCAAGATCGT
CACAGCCCCCAAACCCGCCAGCATGATCCCGAAAAGTATCGGTTGTCCAGAAACCTTCGCCGCCATCGTTACTGCCAAATACGTTGATGCGCTACCGCTA
TACCGTCAGGTTGATATTCTTAAGCGAGGCGACATCGATATTAGCCGAGGTACGCTGGCCAACTGGTGTGTTCAGCTAGGGAACAACGTTGACGTCATTA
TCGACCAGATGAAAGCACGCTTGTTATCTGAAAGCTTAATCTGCGCAGACGAAACCACGGTACAGGTACTGTCAGAGCCAGGGAAAACAGCGCAAAGCAG
GTCCTATATGTGGGTCTATCGCAGTGGCGAGTTCCATAAGTCACCTGTGGTTATCTATGATTATCAGCCAGGGCGCGGGGCAAACTGCCTCAACACCTTC
CTTGGTGATTACTCTGGTTATCTGCTGTCGGATGGGTATCCCGCATATGACACTCTTAAAGATGTCACGCAAGCGGGTTGCATGGCCCATGCAAGACGTA
AGTTCACCGATGCTCAAAAAGCTCAACCGACCAAGAAAGTTGGCCGTGTAGAAAAAGCGCTGAACTATATCGGGAAGCTTTACGGTATAGAACGGGAGAC
TAGAAAACTAAGCCCAGAAAAGCGGCGACAGATAAGGCAACAAAAAGCCAAGCCTATACTCGATGAGTTCCATCAATGGTTGGTCGGAATTAAGGAAACA
CAACTACCGAAAGGTAAGCTCGGGATCGCCATCAACTATGCGATAAACCAGTGGCCAAAACTTCTTACGTATCTTGAAGACGGTCATATCAGCATCGATA
ATAACGTGACTGAGAGAGATGTGCGTCCGTTTACCACGGGGCGCAAAAATTGGATGTTCTCAACTTCAGTAGACGGAGCAAAGGCCAGCGCAAACCTCTA
TAGCCTGGTCATGACTTGCCGTGCCAACGACATCAATCCCTACTTCTACTTCGTGCACCTGTTCAGGGAGTTACCGAAGCGTTCACCAAGCGATGATTTA
ACTGATCTTATGCCATGGAATGTAGAAATCAGCGAGGCTGAATAAGGTCTCACTGGTTAACTAGGCGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 309 bp | 102 aa | 77 | 385 | + | No |
AG : IS66 TnpA
ORF sequence :
MNQSNKTQFWAEIIEQQSQSELSIVAFCKANQLTVNTFYYWRKKLYGGEQQQVVHPVLIEDSPKSIGERQSVMLSLPTGMTVEFPSNMPAHQIQNWLQAL
TC
TC
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 348 bp | 115 aa | 379 | 726 | + | No |
AG : IS66 TnpB
ORF sequence :
MLIPNLGIYLVSGITDMRKSIDGLSLIVADVSEMDPFSEAWFIFCNRGRDKIKILFWDTNGFWLYYRRLEKGRFNWPKPNASGHVAISRQQLQWLLSGLN
LEHPKAHQPLYGLEV
LEHPKAHQPLYGLEV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1530 bp | 509 aa | 816 | 2345 | + | No |
Chemistry : DDE
ORF sequence :
MNHLPDDVEQLKAILLALQEENKAKDRQLQAQQEEMAELKTRVELLLEQLNLSKSKRFSSQSEKVVKGTFNEAEQQNALHKPEPAHHKKGRKPLPEELER
EVQVHTLNAPHCKCCGHELHKCRVETSEELKIIPQKISVIRHERTKYACRQCEHTETSSKIVTAPKPASMIPKSIGCPETFAAIVTAKYVDALPLYRQVD
ILKRGDIDISRGTLANWCVQLGNNVDVIIDQMKARLLSESLICADETTVQVLSEPGKTAQSRSYMWVYRSGEFHKSPVVIYDYQPGRGANCLNTFLGDYS
GYLLSDGYPAYDTLKDVTQAGCMAHARRKFTDAQKAQPTKKVGRVEKALNYIGKLYGIERETRKLSPEKRRQIRQQKAKPILDEFHQWLVGIKETQLPKG
KLGIAINYAINQWPKLLTYLEDGHISIDNNVTERDVRPFTTGRKNWMFSTSVDGAKASANLYSLVMTCRANDINPYFYFVHLFRELPKRSPSDDLTDLMP
WNVEISEAE
EVQVHTLNAPHCKCCGHELHKCRVETSEELKIIPQKISVIRHERTKYACRQCEHTETSSKIVTAPKPASMIPKSIGCPETFAAIVTAKYVDALPLYRQVD
ILKRGDIDISRGTLANWCVQLGNNVDVIIDQMKARLLSESLICADETTVQVLSEPGKTAQSRSYMWVYRSGEFHKSPVVIYDYQPGRGANCLNTFLGDYS
GYLLSDGYPAYDTLKDVTQAGCMAHARRKFTDAQKAQPTKKVGRVEKALNYIGKLYGIERETRKLSPEKRRQIRQQKAKPILDEFHQWLVGIKETQLPKG
KLGIAINYAINQWPKLLTYLEDGHISIDNNVTERDVRPFTTGRKNWMFSTSVDGAKASANLYSLVMTCRANDINPYFYFVHLFRELPKRSPSDDLTDLMP
WNVEISEAE
Blast result :
Comments
ISVme1 is respectively 58%, 82% and 79% aa similar to orfA, orfB and orfC of ISSba7.
References
1] Rowe-Magnus,D.A., Guerout,A.-M. and Mazel,D. (2002) Direct Submission GenBank.