ISDka1
- Family IS607
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Desulfurococcus kamchatkensis | Desulfurococcus kamchatkensis |
DNA section
IS Length : 1999 bp
Ends
IR Length :
IRL : GGGGCAGAAACGTTTATTAAGGTCATTGAGGAAATTTTATAGACAGTTGA
IRR : CCGTTTGTCCCTAGAGGGCTGTTCTGTGCTTTTTAGGGTCATTTAGGGTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATTGGAAAACCCTATAAGA | TAAAGAGGAAAATAGATCCA | 0 |
DNA sequence
GGGGCAGAAACGTTTATTAAGGTCATTGAGGAAATTTTATAGACAGTTGAGGTCGAATGTTGGAGAGGTTGTTGAAGCCTAGGGAGTTCTGCGAGATTGT
TGGGATTAGCTACAGGACTTTTAAGCGGTGGGTCTCTGAAGGCAGAGTGAGCGTCGTGCGAACGCCTAGTGGCAGGATGAGAGTACCGTACTCCGAGGTA
GAGCGCATCCTTGGAGGGAGGCCGGAAGCCAGAGAAGTAAGGGCAGTAGTATATGCCCGCGTGAGCTCCAGCGACCAGAGGAGCGACCTCGAGAGGCAAG
TGCAGTACCTCACCCAGTACTGCTCTGCTAGGGGCTACAGAGTAGTCGACGTCCTAACAGACATCGCCAGCGGGCTGAAGGCAGACCGCAGAGGGCTGCT
CAAGCTCTTCGAATACGTCGTCAGTAAACAAGTAGACGTCGTGGTTATTACATACAGGGACAGGCTGACCAGGTTCGGCTTCGAGTACCTAGAGTACTTC
TTCAGGCAGTACGGCGTTAGGATAGAGGTCATCTATGGTGATGAGCCCAAGGACGCGCGCCAGGAGCTTGTGGAGGATCTACTATCGATAGTCACCTCAT
TTGCTGGCAAGCTCTACGGGCTTAGAAGCCACAGAAAAAAGAGGCTCGTTGAGGGCTTCAAAAAGCTAGTGGAAGAGGTCGAAAAGGGCGGTGGCGAGAG
TAGCTAGAACCATTGTAGTCGAGACGCCGCCCATTCCAAGGAGGCTCTTCAGGGTCTTCGCCGAGCTAGAGGGCATGTACCGGAACATGGTCGAGCAACT
AGTGTTACATGCTGTGAGGGAGGGCGTACCCTCCTTCACGAGGCTCAAGGCGCTGAAGTACCGGGAGCTGAGAAGCCTCTACCCTCAGCTACCCTCACAC
TACGCTTACACCGCGTGCCAGGACGCCTCTGCTAGAGCCAAGAGCTTCCTCAGGCTGAAGAAGAAAGGGCTGGTTGAAAGAGAGCACCCGGAAGTCGACA
GCGTCTCTATCTGGCTGGACGACCATTTGTGGAGAGCAAACGGTTTGACATCTATCGAAGTAGCTACCCATAATGGCTGGATACCTCTCGAAGTTATACC
GCACAAGCAGTACTGGAAGTATGTCAATAAGGGCTGGAGGCTTGCTTCCGAAGCAAAGATAAAGCTGGACAGGAAGAGCAGGAAGTTGCTAGTCTACCTG
ACCTTCGCTAAAGAGGTCGAAGAGTACAAGCCACGCGGCTACGTCTCCGTGGACGTGAACGAGGACAACGTAACGGTTCTAATCGATGGAGTCGCATACC
TCTTCGAGACAAATATGAAGAGAGTGGTCCTCGGCTACTACTACCGCAGGAAGAGGGTGCAGGAGAAGTACGACGTTGTCTACGGGACTAATTTTAGTAG
AAAGAAGAGGATCCTGAGGAAGCTTAAGGAAAAGAAAAAGAAAGACGACGCAAGATGGAAGATCGCGAGCATTATTGTGAGAGAGGCTTTCAAGAGGCGC
TACGCCATAGTCATGGAGAAGCTCGGCAAGAGACCCGGTGAGAAGATGGTCTCCAGGATAAGGGACAGCCAGCTGAGGCACAGGGTGTTTCAGGCATCGT
TTAAGGGCGTGCAGAGGGCTATTGAGGAGAAGGCGAGGGAGTACGGCGTACCAGTAATCTATGTAGATCCCAAGAACACGTCCAGGCTCTGCCCGGTTCA
CGGCTCAAAAATAGTCTACGGGGATGAATCAAGGATAGGCAAGTGCGAGAGAGGCGGTGAAATATGGCACAGGGACGTGGTAGCCGTGTGGAACTTGCTC
CTCAAAACCCTGCGGGGCGGTGGGAGCAGTGCTCCAAGCCCCGCAGGGCTACCCGTAGATGGGAGCCCCGTGCCGTTGGGCTCGACAGCCGCCCATGAAC
CCACGGTGATAGCACGTGACCTGTGGGCGAGGTGGAAGTCCCTAGAGGCGACCCTAAATGACCCTAAAAAGCACAGAACAGCCCTCTAGGGACAAACGG
TGGGATTAGCTACAGGACTTTTAAGCGGTGGGTCTCTGAAGGCAGAGTGAGCGTCGTGCGAACGCCTAGTGGCAGGATGAGAGTACCGTACTCCGAGGTA
GAGCGCATCCTTGGAGGGAGGCCGGAAGCCAGAGAAGTAAGGGCAGTAGTATATGCCCGCGTGAGCTCCAGCGACCAGAGGAGCGACCTCGAGAGGCAAG
TGCAGTACCTCACCCAGTACTGCTCTGCTAGGGGCTACAGAGTAGTCGACGTCCTAACAGACATCGCCAGCGGGCTGAAGGCAGACCGCAGAGGGCTGCT
CAAGCTCTTCGAATACGTCGTCAGTAAACAAGTAGACGTCGTGGTTATTACATACAGGGACAGGCTGACCAGGTTCGGCTTCGAGTACCTAGAGTACTTC
TTCAGGCAGTACGGCGTTAGGATAGAGGTCATCTATGGTGATGAGCCCAAGGACGCGCGCCAGGAGCTTGTGGAGGATCTACTATCGATAGTCACCTCAT
TTGCTGGCAAGCTCTACGGGCTTAGAAGCCACAGAAAAAAGAGGCTCGTTGAGGGCTTCAAAAAGCTAGTGGAAGAGGTCGAAAAGGGCGGTGGCGAGAG
TAGCTAGAACCATTGTAGTCGAGACGCCGCCCATTCCAAGGAGGCTCTTCAGGGTCTTCGCCGAGCTAGAGGGCATGTACCGGAACATGGTCGAGCAACT
AGTGTTACATGCTGTGAGGGAGGGCGTACCCTCCTTCACGAGGCTCAAGGCGCTGAAGTACCGGGAGCTGAGAAGCCTCTACCCTCAGCTACCCTCACAC
TACGCTTACACCGCGTGCCAGGACGCCTCTGCTAGAGCCAAGAGCTTCCTCAGGCTGAAGAAGAAAGGGCTGGTTGAAAGAGAGCACCCGGAAGTCGACA
GCGTCTCTATCTGGCTGGACGACCATTTGTGGAGAGCAAACGGTTTGACATCTATCGAAGTAGCTACCCATAATGGCTGGATACCTCTCGAAGTTATACC
GCACAAGCAGTACTGGAAGTATGTCAATAAGGGCTGGAGGCTTGCTTCCGAAGCAAAGATAAAGCTGGACAGGAAGAGCAGGAAGTTGCTAGTCTACCTG
ACCTTCGCTAAAGAGGTCGAAGAGTACAAGCCACGCGGCTACGTCTCCGTGGACGTGAACGAGGACAACGTAACGGTTCTAATCGATGGAGTCGCATACC
TCTTCGAGACAAATATGAAGAGAGTGGTCCTCGGCTACTACTACCGCAGGAAGAGGGTGCAGGAGAAGTACGACGTTGTCTACGGGACTAATTTTAGTAG
AAAGAAGAGGATCCTGAGGAAGCTTAAGGAAAAGAAAAAGAAAGACGACGCAAGATGGAAGATCGCGAGCATTATTGTGAGAGAGGCTTTCAAGAGGCGC
TACGCCATAGTCATGGAGAAGCTCGGCAAGAGACCCGGTGAGAAGATGGTCTCCAGGATAAGGGACAGCCAGCTGAGGCACAGGGTGTTTCAGGCATCGT
TTAAGGGCGTGCAGAGGGCTATTGAGGAGAAGGCGAGGGAGTACGGCGTACCAGTAATCTATGTAGATCCCAAGAACACGTCCAGGCTCTGCCCGGTTCA
CGGCTCAAAAATAGTCTACGGGGATGAATCAAGGATAGGCAAGTGCGAGAGAGGCGGTGAAATATGGCACAGGGACGTGGTAGCCGTGTGGAACTTGCTC
CTCAAAACCCTGCGGGGCGGTGGGAGCAGTGCTCCAAGCCCCGCAGGGCTACCCGTAGATGGGAGCCCCGTGCCGTTGGGCTCGACAGCCGCCCATGAAC
CCACGGTGATAGCACGTGACCTGTGGGCGAGGTGGAAGTCCCTAGAGGCGACCCTAAATGACCCTAAAAAGCACAGAACAGCCCTCTAGGGACAAACGG
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 651 bp | 216 aa | 57 | 707 | + | No |
Chemistry : Serine
ORF sequence :
MLERLLKPREFCEIVGISYRTFKRWVSEGRVSVVRTPSGRMRVPYSEVERILGGRPEAREVRAVVYARVSSSDQRSDLERQVQYLTQYCSARGYRVVDVL
TDIASGLKADRRGLLKLFEYVVSKQVDVVVITYRDRLTRFGFEYLEYFFRQYGVRIEVIYGDEPKDARQELVEDLLSIVTSFAGKLYGLRSHRKKRLVEG
FKKLVEEVEKGGGESS
TDIASGLKADRRGLLKLFEYVVSKQVDVVVITYRDRLTRFGFEYLEYFFRQYGVRIEVIYGDEPKDARQELVEDLLSIVTSFAGKLYGLRSHRKKRLVEG
FKKLVEEVEKGGGESS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1299 bp | 432 aa | 691 | 1989 | + | No |
AG : TnpB
ORF sequence :
MARVARTIVVETPPIPRRLFRVFAELEGMYRNMVEQLVLHAVREGVPSFTRLKALKYRELRSLYPQLPSHYAYTACQDASARAKSFLRLKKKGLVEREHP
EVDSVSIWLDDHLWRANGLTSIEVATHNGWIPLEVIPHKQYWKYVNKGWRLASEAKIKLDRKSRKLLVYLTFAKEVEEYKPRGYVSVDVNEDNVTVLIDG
VAYLFETNMKRVVLGYYYRRKRVQEKYDVVYGTNFSRKKRILRKLKEKKKKDDARWKIASIIVREAFKRRYAIVMEKLGKRPGEKMVSRIRDSQLRHRVF
QASFKGVQRAIEEKAREYGVPVIYVDPKNTSRLCPVHGSKIVYGDESRIGKCERGGEIWHRDVVAVWNLLLKTLRGGGSSAPSPAGLPVDGSPVPLGSTA
AHEPTVIARDLWARWKSLEATLNDPKKHRTAL
EVDSVSIWLDDHLWRANGLTSIEVATHNGWIPLEVIPHKQYWKYVNKGWRLASEAKIKLDRKSRKLLVYLTFAKEVEEYKPRGYVSVDVNEDNVTVLIDG
VAYLFETNMKRVVLGYYYRRKRVQEKYDVVYGTNFSRKKRILRKLKEKKKKDDARWKIASIIVREAFKRRYAIVMEKLGKRPGEKMVSRIRDSQLRHRVF
QASFKGVQRAIEEKAREYGVPVIYVDPKNTSRLCPVHGSKIVYGDESRIGKCERGGEIWHRDVVAVWNLLLKTLRGGGSSAPSPAGLPVDGSPVPLGSTA
AHEPTVIARDLWARWKSLEATLNDPKKHRTAL
Blast result :
Comments
The genome of crenarchaeon Desulfurococcus kamchatkensis contains six full size copies of this transposon and eight copies of 267 bp-long MITE element derived from it.
ISDka1 is 79% (ORFA) and 62% (ORFB) aa similar to ISC1913.
ISDka1 is 79% (ORFA) and 62% (ORFB) aa similar to ISC1913.
References
1] Ravin N.V., Mardanov A.V., Beletsky A.V., Kublanov I.V., Kolganova T.V., Lebedinsky A.V., Chernyh N.A., Bonch-Osmolovskaya E.A.,Skryabin K.G. (2008) Complete genome sequence of the anaerobic, peptide fermenting hyperthermophilic crenarchaeon Desulfurococcus kamchatkensis (submitted)