ISSpu8
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009438 | ND | Shewanella putrefaciens | Shewanella putrefaciens CN32 |
DNA section
IS Length : 1099 bp
Ends
IR Length : 29/35
IRL : TACCAATTATATTAATTATGTGATCTAATTGGTCATCAGTTTTTAATCAT
IRR : TACCAATTACATTAATTAACTGGTCAAAATTGTCCACGGTCTAGTACACA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCCCAGTGC | TAA | GATTCCAAAA | 3 |
| TCTGGCGCTA | TTA | ATCACTTTTA | 3 |
DNA sequence
TACCAATTATATTAATTATGTGATCTAATTGGTCATCAGTTTTTAATCATACAAAATATGAATCATCCCCACATCGCAGCCTTAGTAAAATCGGAGAAAA
GTGCACGCAAACGTATGCGCTATCTTGCCTTACTCCATTTCACTGAAGGTCATTCTCGTACCGCCATCGCAAGCATGCTTAAGGTCAGTAGAACCAGTGT
CAATCGCTGGGTCACCGCCTATTTAACCCATGGGCTGACTGGCCTTGACGATGCGCCCAATCCGGGCCGTCCTGCGACACTGTCCATCGCACAGCAAGCT
AAGCTTAAAGCGTTCGTACAGCACAGAAGTCTATCTGAACAAGGTGGCAGGTTGATGGCCAAGGATGTCGGCCTATTTATTCAGTCTGAATTTGGTGTGA
CGTTCAAGCAAGCAAATATCTACCGCTTGCTGCATCAACTTGGCTTCTCATGGATTACCACTCGCTCTCGCCACCCGAAACAATCTGAGGCTATACAAGA
GGCTTTTAAAAAACTTCCGGATGGCAACGATCCTTAACACACCTGGGCACGTTGCGCTCGATCGCATTGACGTTTGGTTTCAAGATGAAGCCCGCTTTGG
CCAACAGAATACCACCACCCGTATTTGGGCTGAGAAAGGGACCCGTCCACGGGCCGTAAAGCAGCAACAATTTGAATCAGCGTATCTATATGGCGCCGTG
TGTCCGGCAACGGGGGCGACACAAGCGATTATTGCCCCGCATGCCAATAGCGAATATATGAACGAGCATTTAAAACTGATTTCAACCGCCACCACATTTG
GGCGCCATGCGCTGGTCATCATGGACGGCGCAGGCTGGCATCAACAGGATTTGGCCGATGACTTTGATAATCTGACGCTTCTCAAGTTGCCACCTTATTC
ACCGGAGTTGAATCCAATAGAGCAGGTATGGCAATGGCTGCGTCAAAATGTGTTGGCCAATCGTTGTTTTAGTGGTTATGACGATATTGTCGAACAGTGC
TCTATCGCATGGAATACCTTCATTGAGAAGAAAGCAAGAGTGATAGAATTGTGTACTAGACCGTGGACAATTTTGACCAGTTAATTAATGTAATTGGTA
GTGCACGCAAACGTATGCGCTATCTTGCCTTACTCCATTTCACTGAAGGTCATTCTCGTACCGCCATCGCAAGCATGCTTAAGGTCAGTAGAACCAGTGT
CAATCGCTGGGTCACCGCCTATTTAACCCATGGGCTGACTGGCCTTGACGATGCGCCCAATCCGGGCCGTCCTGCGACACTGTCCATCGCACAGCAAGCT
AAGCTTAAAGCGTTCGTACAGCACAGAAGTCTATCTGAACAAGGTGGCAGGTTGATGGCCAAGGATGTCGGCCTATTTATTCAGTCTGAATTTGGTGTGA
CGTTCAAGCAAGCAAATATCTACCGCTTGCTGCATCAACTTGGCTTCTCATGGATTACCACTCGCTCTCGCCACCCGAAACAATCTGAGGCTATACAAGA
GGCTTTTAAAAAACTTCCGGATGGCAACGATCCTTAACACACCTGGGCACGTTGCGCTCGATCGCATTGACGTTTGGTTTCAAGATGAAGCCCGCTTTGG
CCAACAGAATACCACCACCCGTATTTGGGCTGAGAAAGGGACCCGTCCACGGGCCGTAAAGCAGCAACAATTTGAATCAGCGTATCTATATGGCGCCGTG
TGTCCGGCAACGGGGGCGACACAAGCGATTATTGCCCCGCATGCCAATAGCGAATATATGAACGAGCATTTAAAACTGATTTCAACCGCCACCACATTTG
GGCGCCATGCGCTGGTCATCATGGACGGCGCAGGCTGGCATCAACAGGATTTGGCCGATGACTTTGATAATCTGACGCTTCTCAAGTTGCCACCTTATTC
ACCGGAGTTGAATCCAATAGAGCAGGTATGGCAATGGCTGCGTCAAAATGTGTTGGCCAATCGTTGTTTTAGTGGTTATGACGATATTGTCGAACAGTGC
TCTATCGCATGGAATACCTTCATTGAGAAGAAAGCAAGAGTGATAGAATTGTGTACTAGACCGTGGACAATTTTGACCAGTTAATTAATGTAATTGGTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 480 bp | 159 aa | 58 | 537 | + | No |
Description : First part of the transposase
ORF sequence :
MNHPHIAALVKSEKSARKRMRYLALLHFTEGHSRTAIASMLKVSRTSVNRWVTAYLTHGLTGLDDAPNPGRPATLSIAQQAKLKAFVQHRSLSEQGGRLM
AKDVGLFIQSEFGVTFKQANIYRLLHQLGFSWITTRSRHPKQSEAIQEAFKKLPDGNDP
AKDVGLFIQSEFGVTFKQANIYRLLHQLGFSWITTRSRHPKQSEAIQEAFKKLPDGNDP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 684 bp | 227 aa | 401 | 1084 | + | No |
Description : Second part of the transposase
ORF sequence :
RSSKQISTACCINLASHGLPLALATRNNLRLYKRLLKNFRMATILNTPGHVALDRIDVWFQDEARFGQQNTTTRIWAEKGTRPRAVKQQQFESAYLYGAV
CPATGATQAIIAPHANSEYMNEHLKLISTATTFGRHALVIMDGAGWHQQDLADDFDNLTLLKLPPYSPELNPIEQVWQWLRQNVLANRCFSGYDDIVEQC
SIAWNTFIEKKARVIELCTRPWTILTS
CPATGATQAIIAPHANSEYMNEHLKLISTATTFGRHALVIMDGAGWHQQDLADDFDNLTLLKLPPYSPELNPIEQVWQWLRQNVLANRCFSGYDDIVEQC
SIAWNTFIEKKARVIELCTRPWTILTS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1027 bp | 341 aa | 58 | 1084 | + | Yes |
Chemistry : DDE
ORF sequence :
MNHPHIAALVKSEKSARKRMRYLALLHFTEGHSRTAIASMLKVSRTSVNRWVTAYLTHGLTGLDDAPNPGRPATLSIAQQAKLKAFVQHRSLSEQGGRLM
AKDVGLFIQSEFGVTFKQANIYRLLHQLGFSWITTRSRHPKQSEAIQEAFKNFRMATILNTPGHVALDRIDVWFQDEARFGQQNTTTRIWAEKGTRPRAV
KQQQFESAYLYGAVCPATGATQAIIAPHANSEYMNEHLKLISTATTFGRHALVIMDGAGWHQQDLADDFDNLTLLKLPPYSPELNPIEQVWQWLRQNVLA
NRCFSGYDDIVEQCSIAWNTFIEKKARVIELCTRPWTILTS
AKDVGLFIQSEFGVTFKQANIYRLLHQLGFSWITTRSRHPKQSEAIQEAFKNFRMATILNTPGHVALDRIDVWFQDEARFGQQNTTTRIWAEKGTRPRAV
KQQQFESAYLYGAVCPATGATQAIIAPHANSEYMNEHLKLISTATTFGRHALVIMDGAGWHQQDLADDFDNLTLLKLPPYSPELNPIEQVWQWLRQNVLA
NRCFSGYDDIVEQCSIAWNTFIEKKARVIELCTRPWTILTS
Blast result :
Comments
There are 2 copies of ISSpu8 encoded in the Shewanella putrefaciens CN-32 genome.
The 3rd ORF is an in silico programmed frameshift of ORF1 and ORF2.
The ORF3 protein has 83% identity to that encoded by ISShrf2.
The 3rd ORF is an in silico programmed frameshift of ORF1 and ORF2.
The ORF3 protein has 83% identity to that encoded by ISShrf2.
References
1] Romine, M. (2008) Direct submission.