ISSpu15
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008750 | ND | Shewanella putrefaciens | Shewanella putrefaciens W3-18-1 |
DNA section
IS Length : 2420 bp
Ends
IR Length : 43/53
IRL : TATTGAACGACAATTAACTTGTCCGGTCGGCGACAACTATGTTGGCCGGT
IRR : TATTGAACGTCAACTACCTTGTCCGGTTCGCGTCAATTAACTTGTCCGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGGATCTCT | AATGAA | TTTGACTCTC | 6 |
DNA sequence
TATTGAACGACAATTAACTTGTCCGGTCGGCGACAACTATGTTGGCCGGTTGATCTGATACCGTCCGTCACGGATTCCATTAGGAGATAACCGTGTCGGG
CAAACCCATCACAGATCAACAAATCAGGTTATATGTGAATGAACGTAAACAAGGTCAAACCCAGCTTGCCGCTTCGGCAAAGTCTGGTTTATCAGAGCGA
TCAGGTCGCCGCATAGACAGCGGTGAACTGAACACAGAACCAACCCCCAAGCGTCAATGGCGCACTCGCAAAGACCCGCTTGCTGAGGTCTGGTCATCGG
TGCTGGTACCACTACTCGAAGCGAACCCTGAACTATTGCCGCTCACGTTGTTTGAATACCTTCACGACAACTACCCCAATCAGTATGACAACACCGTACT
GCGCACGTTGCAGCGACGTGTCAAAGCATGGAAGGTCAAACATGGCCCCGCAAAGGAAGTCATGTTCCGCCAAACTAAAGTGCCTGGGCGTTTGGGTTTA
TCGGACTTCACGCTCTTGAAAGGGGTTACGATTACAATACTTGGGCAGGTATTTTGCCATTTGCTATACCACTATCGCCTTGCCTTTAGCGGCTGGACGT
ACGCCAAAGTCATTTGCGGTGGTGAAAGCTATACCGCCTTGTCTACAGGGCTTCAGAATGCCTTATGGCGCAGTGGTGGCGCACCCCTTGAACACCGCAC
CGACAGCTTGAGTGCCGCCTTTAACAATCTGGCCGAGAAGGAACAGCTTACCGTTCGGTACGACGCCTTATGCCACCACTATCAACTCAAAGGCAGTCGT
AACAACCCCGGCAAGAGCCATGAGAACGGTACAATAGAAGCGGCACACGGGCATTTAAAGCGACGTATGACTCAGGCCTTGCTGCTGCGAGGCGATACTG
ATTTTACGTCCCTTGAGGCCTATCAAGCCTTCGTGGATGGTATTGTCACCAAGATTAACCAGCAATGCCGCACCCGCTTTGAACAAGAGCGGCCGTTATT
GCAGACACTGCCAAAACGGCGCACTCATGATTATGCGGAGCACAGTGTTCTGATTAGCAGCAGTAGCAGCTTTGACCTCAAACGGGTGACCTATACCGTA
CCGTCTCGCTTTATCGGCGAGCGGCTGTATGTTCAGCTGTATGACGAGCGCTTGGATCTGTTCAGTGGGCATGAGCAGATACTCAGCTTACCGCGTGTTT
ATGCCACCACAACCCAGCGCGGACGCAGTGTCGATTATCGCCATGTTATCGACTCCTTGGTTAAAAAGCCGGGGGCCTTCCGTTACTCGCAGCTGCGCGA
TGACTTATTACCCACCCCGGACTACCACCGTATCTGGCAGTATGTTGACGGCACACTGAATCCCCATGATGCCTGCCGTTACATCGTGCGCCTGCTGCAC
TTGGCCGCGACAGCTCAGTGCCAAGATAGCCTTGGTCGTTACGTACTCAAAAGCATTGAGGGGGGTGAATTACCCAGCAACTTACAGTGCAATAAGCGCT
TTAGTACAGACACGGCCGTGATTCCACTCATTACGGCAAAGCAGCACCTGCTGGCTGATTACGATCAGCTGTTATGTGTGGAGGTGCAACATGGCTGATC
TTGCCTCCCTCCCGATTCTCTTGAAGGAATTAAAGTTAAGTAGCTTCGCCCAGCACTGGGAAGGCCTTGCACAAAAGGCGCTGGATGAACAATGGTTACC
GCAAACCTACCTTGCGGCTTTGTGTGAACACGAAGTGGGTGAGCGTCATCACAAGCGTTTACAACGCTATACCCGCGAAGCACAACTGCCACCGGGTAAG
CCGTTGAGCCAATTTGATTTTAGTGTGGTAGAGGGGATTAATAAAAATCAGCTTATTGCCCTGGCGGATCAACAGCAGTGGGTTAATCAAGCCGAGAACG
TGTTGTTGTTTGGTGCCAGTGGTGTGGGTAAAACCCATCTTGCCTGTGGTATTGGCTATGCCTTGTTGGCGCAAGGGGTGCGAGTAAAGTTCACTAGCGC
TACCCATCTTGCTCAAAGCCTTCAGCAAGCAAAAGCGGCGTTGGTATTGACTGACGTGCTGGCTCGCATGGACAAATATACGGTGCTCATCATTGATGAT
ATTGGCTACGTCAAAAAAAGCAGCCAAGAAACACAGGTATTGTTTGAATTGATTGCGCACCGCTATGAAACGGGCAGTTTGATTATCACCTCAAATCAGC
CTTTTAGTGGGTGGGATCAGATCTTCGACGACAACATGATGACGGTGGCGGCGATTGATCGGTTAGTGCATCACGCCACTATTTTAGAGATAGGTGGCGA
AAGCTACCGTAAAAAGGCGTCAATGATGAGAAAGGAGATGCCTATCGCTAACGACAATTAAACATCGTCAACCGGACAAGTTAATTGACGCGAACCGGAC
AAGGTAGTTGACGTTCAATA
CAAACCCATCACAGATCAACAAATCAGGTTATATGTGAATGAACGTAAACAAGGTCAAACCCAGCTTGCCGCTTCGGCAAAGTCTGGTTTATCAGAGCGA
TCAGGTCGCCGCATAGACAGCGGTGAACTGAACACAGAACCAACCCCCAAGCGTCAATGGCGCACTCGCAAAGACCCGCTTGCTGAGGTCTGGTCATCGG
TGCTGGTACCACTACTCGAAGCGAACCCTGAACTATTGCCGCTCACGTTGTTTGAATACCTTCACGACAACTACCCCAATCAGTATGACAACACCGTACT
GCGCACGTTGCAGCGACGTGTCAAAGCATGGAAGGTCAAACATGGCCCCGCAAAGGAAGTCATGTTCCGCCAAACTAAAGTGCCTGGGCGTTTGGGTTTA
TCGGACTTCACGCTCTTGAAAGGGGTTACGATTACAATACTTGGGCAGGTATTTTGCCATTTGCTATACCACTATCGCCTTGCCTTTAGCGGCTGGACGT
ACGCCAAAGTCATTTGCGGTGGTGAAAGCTATACCGCCTTGTCTACAGGGCTTCAGAATGCCTTATGGCGCAGTGGTGGCGCACCCCTTGAACACCGCAC
CGACAGCTTGAGTGCCGCCTTTAACAATCTGGCCGAGAAGGAACAGCTTACCGTTCGGTACGACGCCTTATGCCACCACTATCAACTCAAAGGCAGTCGT
AACAACCCCGGCAAGAGCCATGAGAACGGTACAATAGAAGCGGCACACGGGCATTTAAAGCGACGTATGACTCAGGCCTTGCTGCTGCGAGGCGATACTG
ATTTTACGTCCCTTGAGGCCTATCAAGCCTTCGTGGATGGTATTGTCACCAAGATTAACCAGCAATGCCGCACCCGCTTTGAACAAGAGCGGCCGTTATT
GCAGACACTGCCAAAACGGCGCACTCATGATTATGCGGAGCACAGTGTTCTGATTAGCAGCAGTAGCAGCTTTGACCTCAAACGGGTGACCTATACCGTA
CCGTCTCGCTTTATCGGCGAGCGGCTGTATGTTCAGCTGTATGACGAGCGCTTGGATCTGTTCAGTGGGCATGAGCAGATACTCAGCTTACCGCGTGTTT
ATGCCACCACAACCCAGCGCGGACGCAGTGTCGATTATCGCCATGTTATCGACTCCTTGGTTAAAAAGCCGGGGGCCTTCCGTTACTCGCAGCTGCGCGA
TGACTTATTACCCACCCCGGACTACCACCGTATCTGGCAGTATGTTGACGGCACACTGAATCCCCATGATGCCTGCCGTTACATCGTGCGCCTGCTGCAC
TTGGCCGCGACAGCTCAGTGCCAAGATAGCCTTGGTCGTTACGTACTCAAAAGCATTGAGGGGGGTGAATTACCCAGCAACTTACAGTGCAATAAGCGCT
TTAGTACAGACACGGCCGTGATTCCACTCATTACGGCAAAGCAGCACCTGCTGGCTGATTACGATCAGCTGTTATGTGTGGAGGTGCAACATGGCTGATC
TTGCCTCCCTCCCGATTCTCTTGAAGGAATTAAAGTTAAGTAGCTTCGCCCAGCACTGGGAAGGCCTTGCACAAAAGGCGCTGGATGAACAATGGTTACC
GCAAACCTACCTTGCGGCTTTGTGTGAACACGAAGTGGGTGAGCGTCATCACAAGCGTTTACAACGCTATACCCGCGAAGCACAACTGCCACCGGGTAAG
CCGTTGAGCCAATTTGATTTTAGTGTGGTAGAGGGGATTAATAAAAATCAGCTTATTGCCCTGGCGGATCAACAGCAGTGGGTTAATCAAGCCGAGAACG
TGTTGTTGTTTGGTGCCAGTGGTGTGGGTAAAACCCATCTTGCCTGTGGTATTGGCTATGCCTTGTTGGCGCAAGGGGTGCGAGTAAAGTTCACTAGCGC
TACCCATCTTGCTCAAAGCCTTCAGCAAGCAAAAGCGGCGTTGGTATTGACTGACGTGCTGGCTCGCATGGACAAATATACGGTGCTCATCATTGATGAT
ATTGGCTACGTCAAAAAAAGCAGCCAAGAAACACAGGTATTGTTTGAATTGATTGCGCACCGCTATGAAACGGGCAGTTTGATTATCACCTCAAATCAGC
CTTTTAGTGGGTGGGATCAGATCTTCGACGACAACATGATGACGGTGGCGGCGATTGATCGGTTAGTGCATCACGCCACTATTTTAGAGATAGGTGGCGA
AAGCTACCGTAAAAAGGCGTCAATGATGAGAAAGGAGATGCCTATCGCTAACGACAATTAAACATCGTCAACCGGACAAGTTAATTGACGCGAACCGGAC
AAGGTAGTTGACGTTCAATA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1506 bp | 501 aa | 93 | 1598 | + | No |
Chemistry : DDE
ORF sequence :
VSGKPITDQQIRLYVNERKQGQTQLAASAKSGLSERSGRRIDSGELNTEPTPKRQWRTRKDPLAEVWSSVLVPLLEANPELLPLTLFEYLHDNYPNQYDN
TVLRTLQRRVKAWKVKHGPAKEVMFRQTKVPGRLGLSDFTLLKGVTITILGQVFCHLLYHYRLAFSGWTYAKVICGGESYTALSTGLQNALWRSGGAPLE
HRTDSLSAAFNNLAEKEQLTVRYDALCHHYQLKGSRNNPGKSHENGTIEAAHGHLKRRMTQALLLRGDTDFTSLEAYQAFVDGIVTKINQQCRTRFEQER
PLLQTLPKRRTHDYAEHSVLISSSSSFDLKRVTYTVPSRFIGERLYVQLYDERLDLFSGHEQILSLPRVYATTTQRGRSVDYRHVIDSLVKKPGAFRYSQ
LRDDLLPTPDYHRIWQYVDGTLNPHDACRYIVRLLHLAATAQCQDSLGRYVLKSIEGGELPSNLQCNKRFSTDTAVIPLITAKQHLLADYDQLLCVEVQH
G
TVLRTLQRRVKAWKVKHGPAKEVMFRQTKVPGRLGLSDFTLLKGVTITILGQVFCHLLYHYRLAFSGWTYAKVICGGESYTALSTGLQNALWRSGGAPLE
HRTDSLSAAFNNLAEKEQLTVRYDALCHHYQLKGSRNNPGKSHENGTIEAAHGHLKRRMTQALLLRGDTDFTSLEAYQAFVDGIVTKINQQCRTRFEQER
PLLQTLPKRRTHDYAEHSVLISSSSSFDLKRVTYTVPSRFIGERLYVQLYDERLDLFSGHEQILSLPRVYATTTQRGRSVDYRHVIDSLVKKPGAFRYSQ
LRDDLLPTPDYHRIWQYVDGTLNPHDACRYIVRLLHLAATAQCQDSLGRYVLKSIEGGELPSNLQCNKRFSTDTAVIPLITAKQHLLADYDQLLCVEVQH
G
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 771 bp | 256 aa | 1591 | 2361 | + | No |
AG : IS21 helper
ORF sequence :
MADLASLPILLKELKLSSFAQHWEGLAQKALDEQWLPQTYLAALCEHEVGERHHKRLQRYTREAQLPPGKPLSQFDFSVVEGINKNQLIALADQQQWVNQ
AENVLLFGASGVGKTHLACGIGYALLAQGVRVKFTSATHLAQSLQQAKAALVLTDVLARMDKYTVLIIDDIGYVKKSSQETQVLFELIAHRYETGSLIIT
SNQPFSGWDQIFDDNMMTVAAIDRLVHHATILEIGGESYRKKASMMRKEMPIANDN
AENVLLFGASGVGKTHLACGIGYALLAQGVRVKFTSATHLAQSLQQAKAALVLTDVLARMDKYTVLIIDDIGYVKKSSQETQVLFELIAHRYETGSLIIT
SNQPFSGWDQIFDDNMMTVAAIDRLVHHATILEIGGESYRKKASMMRKEMPIANDN
Blast result :
Comments
The only copy of ISSpu15 encoded in S. putrefaciens W3-18-1 is interrupted by ISShes1.
ISShes1 and the flanking duplication which resulted from its insertion was removed to produce the ISSpu15 sequence posted here. ORF1 has 46% identity to ISEc10_aa1 and ORF2 has 57% identity to ISSba11_aa2.
ISShes1 and the flanking duplication which resulted from its insertion was removed to produce the ISSpu15 sequence posted here. ORF1 has 46% identity to ISEc10_aa1 and ORF2 has 57% identity to ISSba11_aa2.
References
1] Romine, M.F. (2008) Direct submission.