ISfinder Mirror Site

ISRosp4

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AANB00000000	ND	Roseobacter sp.	Roseobacter sp. MED193

DNA section

IS Length : 2709 bp

Ends

IR Length : 20/24

IRL : GTACGCGCCGTCTGCGTCCCATTGATTGCTGTGGTTCCAGTGGGCTGATC
IRR : GTATGCGACGTCCACGTCCCATTGGGATTTCGCTTGCGTGTGGGATGACC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAGACTTTCT	TAAGAGAC	TGACGCGAAA	8

DNA sequence

GTACGCGCCGTCTGCGTCCCATTGATTGCTGTGGTTCCAGTGGGCTGATCTAGCGGCGATGTTTCTATTCTGAGCAGTCAGAAGGAGACGACGATGGCAG
ATGGGGGCGACGGTTTTGATGGGTGCGTCGAGGTTGTGCAGCGGACGCGTGGATACCGGCGCTGGCCGGACGAGGTGAAAGGCCGGATCGTGGCCGAAAG
CCTTCAGCCTGGCGTGCGGGTGGTAGATGTCGCGCGGCGGCATGGCCTTGCGGCGCATCAGCTTTCGGATTGGCGGCGTCAGGCGCGGCTGGGCGAACTG
GTTTTGCCTGCGGACGCTATGACCGGGGCGGCGTCTGACGCTCTGCCCGCCTTTGTGCCGATTTCGGTGGGGTCAGATGAGGTCGGGCGGTCAGCGGATA
CCGGTCGGCACAGCGGTGTCATCACGATCGAGATCGGCAACGCGTTCGTGCTGCAGGTGCCGGGCGATGTTCCGGTGGACCGGGCTGCGGCGGTGGCGTG
CGCCCTGCGAGGTTCGGCATGATCGTCGCGGGCCAGCGGCTGCCGATCCTGATCGCGACGAAGCCGGTCGACTTCAGGTGCGGCCACAACGCTCTGGCGC
TGATCGTACAGACCGAGCTGAAGCTTGATCCGCATTCCGGGGTGACGGTGGTGTTCCGCTCGAAGCGCGGGGACAGGTTGAAGATCCTGGTCTGGGACGG
AACCGGGATGGTGCTGATCTACAAGATCCTGGAGCAGGGCAGCTTTGCCTGGCCGAAGGTGCAGGACGGTACGATGCGTCTGTCGCGGGCGCAGGCGGAA
GCCCTGTTCGAAGGGCTCGATTGGCGGCGGGTGGTGGCGCAAAGGGTCGTGCCGCCAGCTGCGGCGGGATGACTCATCAGGCATATATTGGCCATGTTTT
ATTGGGATTTTAAGCCTGGATCGGGTAGAAAGGCGCATGTCACAATCGCTCGATCTCAGCCAGTTCCCAGACCTGCCGCCCGAGGTAGTAAAGGCCTTTG
CGGCCGTTGAATTCGAGCTTTCGGTTGAACGTGCGGCGCGCCAACACGAACAGGCGGTGGCGGCCGAGAAGGAGGCCTTCATCGCCGAGCTGAAGGACCT
GATCGCAAAGCTGGAAGGTCAGGTTCAGGATTACCGGCGCACGAAGTTCGGGCCGAAATCGGAGAAGCTGGACCCAGCGCAGCTGGAGCTGGCGCTGGAG
GATCAGGAAACAGCCATCGCCGAGACGCAGGCGCAGATCGCCGCTCTCGAGGACAGGATGGCAGCAAGTGAGCGTGATCCCGAGAAGCGCAAGTCGCGCG
CCCCCCGCAAGGCACGGGCCTTGCCGGACAGCCTGCCGAGGGTCGAACGCGTGGTTGAACCCGACAGCATCCTATGCCCCTGCGGCTGTGGCGACATGGT
CCGGATCGGTGAAGACCGCACTGAGCGGCTGGATTACATCCCCGCACGCTACCAGGTGGTCGTGACGATCCGGCCCCGCTACGCTTGCCCAAAGGGGCGC
ACCGGCGTGGTGCAGGCCAAGGCACCCGCCCATCTTCTCGAAGGAAGCTGGCCCACCGAGGCGCTGCTGGCCCAGGTCGCCGTGTCCAAGCATTCCGAGC
ACATGCCACTGAACCGGCAGGCCGTGGTGATGGCCCGGCACGGCGTGCCGATCGACCGCTCGGTACTGGCCGACTGGATGGGCCGGACGGGCGCGCTGAT
CGCCCCGGTCGTCGATCACATGGCCAAGCAGCTGCTGACTGGGAGCACCCGGCTTTATGTTGATGAGACCACCGCCCCGGTGTTGGACCCGGGGCGCGGC
AAGACAAAGACGGGATATCTCTGGGCGGTGCTGCGCGATGATCGTGGTTGGAACGGCCCTGCACCGCCCGGCGTGGTGTTCCACTACCGGCCCGGCCGGA
AGGGCGAGTATGCCGCTGAAATCCTCACCGGCTTCAACGGCACGATCCAGGTTGATGCCTATGGTGCCTATACCCACCTGGCCACGCCGAAACGCCCAGG
CGGCGATCCGTTGCGCCTGGCCTTCTGCTGGGCCCATGGGCGGCGCAAACTGATCAAGGCCGCGCCCAAGAAGGGATCACCCATCGTGGACGCGGCGCTG
CTGCGCATCGCGGCGCTCTACAAGGTCGAGGATGCCATCCGCGGGTCAGACCCGGATCATCGCCGGGCTGTCCGGCAGGACCTGTCTCGCCCGCTGGTCG
ATGAGTTCTTCACCTGGCTGGCGGCCCAGGCCGCGCGCGTGTCGCGCAAATCAGACCTCGGCGAGGCAATGGCCTACATGCTGAAACGTCAGGACGGCTT
CCGGCTGTTCCTGGACGACGGCCGCGTCGACATCGACTCCAACCTCGTCGAAAATGCCATCCGCAGCCCGGCCATGAACCGCCGCAACGCGCTCTTCGCC
GGCCATGACGAGGGTGGACGCAACTGGGCCCGCTTTGCCAGTCTGATCGGCACCTGCAAGATGAACGGCGTCGAACCCTATGCCTATCTGCGTGATCTGT
TCACCAAGCTCGCCAACGGGCATCTCGACAAAGACATCGACGCGTTGATGCCGTGGGCTTACGCCGCGGTCCCCGTCCCCTCACAATGAGCTCATCCGGG
AATCCCCACTGAGCTCATTCTGAGCGCTCACAAAGGCGAAAACGTCAATCGAGAGCGCTGGTCATCCCACACGCAAGCGAAATCCCAATGGGACGTGGAC
GTCGCATAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
429 bp	142 aa	94	522	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MADGGDGFDGCVEVVQRTRGYRRWPDEVKGRIVAESLQPGVRVVDVARRHGLAAHQLSDWRRQARLGELVLPADAMTGAASDALPAFVPISVGSDEVGRS
ADTGRHSGVITIEIGNAFVLQVPGDVPVDRAAAVACALRGSA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
354 bp	117 aa	519	872	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIVAGQRLPILIATKPVDFRCGHNALALIVQTELKLDPHSGVTVVFRSKRGDRLKILVWDGTGMVLIYKILEQGSFAWPKVQDGTMRLSRAQAEALFEGL
DWRRVVAQRVVPPAAAG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1653 bp	550 aa	937	2589	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSQSLDLSQFPDLPPEVVKAFAAVEFELSVERAARQHEQAVAAEKEAFIAELKDLIAKLEGQVQDYRRTKFGPKSEKLDPAQLELALEDQETAIAETQAQ
IAALEDRMAASERDPEKRKSRAPRKARALPDSLPRVERVVEPDSILCPCGCGDMVRIGEDRTERLDYIPARYQVVVTIRPRYACPKGRTGVVQAKAPAHL
LEGSWPTEALLAQVAVSKHSEHMPLNRQAVVMARHGVPIDRSVLADWMGRTGALIAPVVDHMAKQLLTGSTRLYVDETTAPVLDPGRGKTKTGYLWAVLR
DDRGWNGPAPPGVVFHYRPGRKGEYAAEILTGFNGTIQVDAYGAYTHLATPKRPGGDPLRLAFCWAHGRRKLIKAAPKKGSPIVDAALLRIAALYKVEDA
IRGSDPDHRRAVRQDLSRPLVDEFFTWLAAQAARVSRKSDLGEAMAYMLKRQDGFRLFLDDGRVDIDSNLVENAIRSPAMNRRNALFAGHDEGGRNWARF
ASLIGTCKMNGVEPYAYLRDLFTKLANGHLDKDIDALMPWAYAAVPVPSQ

Blast result :

Comments

orfA/B/C of ISRosp4 are respectively 69%, 94% and 92% aa similar to orfA/B/C of ISRm14.

References

1] ISfinder annotation (2008).
2] Pinhassi,J., Pedros-Alio,C., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.