ISVch7
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AB012957 | ND | Vibrio cholerae | Vibrio cholerae |
DNA section
IS Length : 1588 bp
Ends
IR Length : 10/14
IRL : GTAAACCGTCAACAAGAACCCAATGAAAACAAAAAAAGGGGAACAATAAC
IRR : GTAAGCGTTCACCAGTAGCATGTTGACTAGGTATTTAGATAAAAACTCGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGTCCTGCCA | CTTGCACT | GGCCCTAGCG | 8 |
DNA sequence
GTAAACCGTCAACAAGAACCCAATGAAAACAAAAAAAGGGGAACAATAACAACGGAACCCAATTTGCCCAATGAAGGAACAATCCCAACCGGCAAGCCGA
ACTGCATGACTAAGAAAAAATCAAAATTCTCATCCTCACCACCTATTGCCTCAGACATTAACGAAGCGAATGCGTTGATATCCGAGTTATGGGAACAGTT
GCGCCATTATGAAGATAGGCTACAAACAAGCAGCGCTAATTCCTCTAAGTCTCCTTCCTCCGATGGACCCAAAGAGCGAGCTGAACGAAAAAAGCTCAAA
GCTCTAGTGGTCGCCATCCGAGAGGAGCTAAACAAGGTCACGAAGGGCATAAACGACAACTCTCAGAACTAAAAGCATCCGATACGGTTGTTGATTGTTA
CCCCGAAAGTACATGCCCTTGCTGCGGTGACGCTGACCTCAAAATGAGTGACAAACCATTTTATCGCCACCAAGTTCATGAAATCCCAGCGCCACGTATC
GACATTACCGAATATCGGCTCTATTCAGGTAAATGCGCGCAATGTGGAGAGGCCGTGAAAGCCCAGAAACCTAGCGATATCCCTCAAGGGATAATGGGGC
CAAACCTAATGAGCCAAATCGCCATACTGGCTGGTCAATATCATCTCAGTGTTCGGAAAATACGCTCACTACTGCAAGAGCAGTTTGGTACTACTTTCTC
AGTGGGTGCCATAAGTGAAGCACAAAGCCGCATTGCTTCTATGCTGACACCACTGCATCAAGCTATCAAAACCAACATACAATCTGCGCCACTAGTCCAT
GTAGATGAAACGTCGCACTCTAGAAATGATGAGAAAGGTTTGCGTTGGTGCTGGCTAGTGGCAAGCGAGGATTTAGTCTATGAAAAAATCCTTTACTCTC
GTTCAATGCATTCAGCAAAAACGGTATTGGACTCAGACTATTGTGGCATTGTGGTCAGTGACCAATATAGCGGTTACAACTGGCTTTCTCCGGACAGACA
CCAACTCTGTTGGGCGCATGTCATTCGCAATCTACAACAAATTGCCGATTACACAGGCAAAGGGCACACCGCAAAAATAGGTCAGCGCCTTGTACTGCTG
AGCAAATTAGTATTTCGAACTCGGCACCGCTGGGAATCTAGCCAAATCGATGACGCTCTCTACCTAAATCGATTGAATCGCATACGTTGTCGTTTTAATC
ATTGGCTGGAAAAAGGGGCAACCCAAATCCCAATTCAATGCTATCAAGGACGGTGTCGAAAGCTTAAAGAGCATAGCCAAAGTTTATGGCTGTTCTTAAC
CAACCCTAAAATCCCTCTGACCAACAACGAAGCAGAGCGACGATTGCGCGGCTTCGTCATTCAGAGAAAAATCAGCTACGGTACCACCTCGGACGCAGGC
GATAAGTTCCGAGACCGTCTACACTCGCTAATCGAAACGTGTAAAAAGCGGAAAATATCGTCACTTGATACGCTTTCACGCATAGCCAACGCGGTGGTTC
GTCAGCAGCCATACCCAAATGTGTTCGACCTTAAGCACACGAGTTTTTATCTAAATACCTAGTCAACATGCTACTGGTGAACGCTTAC
ACTGCATGACTAAGAAAAAATCAAAATTCTCATCCTCACCACCTATTGCCTCAGACATTAACGAAGCGAATGCGTTGATATCCGAGTTATGGGAACAGTT
GCGCCATTATGAAGATAGGCTACAAACAAGCAGCGCTAATTCCTCTAAGTCTCCTTCCTCCGATGGACCCAAAGAGCGAGCTGAACGAAAAAAGCTCAAA
GCTCTAGTGGTCGCCATCCGAGAGGAGCTAAACAAGGTCACGAAGGGCATAAACGACAACTCTCAGAACTAAAAGCATCCGATACGGTTGTTGATTGTTA
CCCCGAAAGTACATGCCCTTGCTGCGGTGACGCTGACCTCAAAATGAGTGACAAACCATTTTATCGCCACCAAGTTCATGAAATCCCAGCGCCACGTATC
GACATTACCGAATATCGGCTCTATTCAGGTAAATGCGCGCAATGTGGAGAGGCCGTGAAAGCCCAGAAACCTAGCGATATCCCTCAAGGGATAATGGGGC
CAAACCTAATGAGCCAAATCGCCATACTGGCTGGTCAATATCATCTCAGTGTTCGGAAAATACGCTCACTACTGCAAGAGCAGTTTGGTACTACTTTCTC
AGTGGGTGCCATAAGTGAAGCACAAAGCCGCATTGCTTCTATGCTGACACCACTGCATCAAGCTATCAAAACCAACATACAATCTGCGCCACTAGTCCAT
GTAGATGAAACGTCGCACTCTAGAAATGATGAGAAAGGTTTGCGTTGGTGCTGGCTAGTGGCAAGCGAGGATTTAGTCTATGAAAAAATCCTTTACTCTC
GTTCAATGCATTCAGCAAAAACGGTATTGGACTCAGACTATTGTGGCATTGTGGTCAGTGACCAATATAGCGGTTACAACTGGCTTTCTCCGGACAGACA
CCAACTCTGTTGGGCGCATGTCATTCGCAATCTACAACAAATTGCCGATTACACAGGCAAAGGGCACACCGCAAAAATAGGTCAGCGCCTTGTACTGCTG
AGCAAATTAGTATTTCGAACTCGGCACCGCTGGGAATCTAGCCAAATCGATGACGCTCTCTACCTAAATCGATTGAATCGCATACGTTGTCGTTTTAATC
ATTGGCTGGAAAAAGGGGCAACCCAAATCCCAATTCAATGCTATCAAGGACGGTGTCGAAAGCTTAAAGAGCATAGCCAAAGTTTATGGCTGTTCTTAAC
CAACCCTAAAATCCCTCTGACCAACAACGAAGCAGAGCGACGATTGCGCGGCTTCGTCATTCAGAGAAAAATCAGCTACGGTACCACCTCGGACGCAGGC
GATAAGTTCCGAGACCGTCTACACTCGCTAATCGAAACGTGTAAAAAGCGGAAAATATCGTCACTTGATACGCTTTCACGCATAGCCAACGCGGTGGTTC
GTCAGCAGCCATACCCAAATGTGTTCGACCTTAAGCACACGAGTTTTTATCTAAATACCTAGTCAACATGCTACTGGTGAACGCTTAC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 106 | 372 | + | No |
Chemistry : DDE
ORF sequence :
MTKKKSKFSSSPPIASDINEANALISELWEQLRHYEDRLQTSSANSSKSPSSDGPKERAERKKLKALVVAIREELNKVTKGINDNSQN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1278 bp | 425 aa | 285 | 1562 | + | No |
Chemistry : DDE
ORF sequence :
TKKAQSSSGRHPRGAKQGHEGHKRQLSELKASDTVVDCYPESTCPCCGDADLKMSDKPFYRHQVHEIPAPRIDITEYRLYSGKCAQCGEAVKAQKPSDIP
QGIMGPNLMSQIAILAGQYHLSVRKIRSLLQEQFGTTFSVGAISEAQSRIASMLTPLHQAIKTNIQSAPLVHVDETSHSRNDEKGLRWCWLVASEDLVYE
KILYSRSMHSAKTVLDSDYCGIVVSDQYSGYNWLSPDRHQLCWAHVIRNLQQIADYTGKGHTAKIGQRLVLLSKLVFRTRHRWESSQIDDALYLNRLNRI
RCRFNHWLEKGATQIPIQCYQGRCRKLKEHSQSLWLFLTNPKIPLTNNEAERRLRGFVIQRKISYGTTSDAGDKFRDRLHSLIETCKKRKISSLDTLSRI
ANAVVRQQPYPNVFDLKHTSFYLNT
QGIMGPNLMSQIAILAGQYHLSVRKIRSLLQEQFGTTFSVGAISEAQSRIASMLTPLHQAIKTNIQSAPLVHVDETSHSRNDEKGLRWCWLVASEDLVYE
KILYSRSMHSAKTVLDSDYCGIVVSDQYSGYNWLSPDRHQLCWAHVIRNLQQIADYTGKGHTAKIGQRLVLLSKLVFRTRHRWESSQIDDALYLNRLNRI
RCRFNHWLEKGATQIPIQCYQGRCRKLKEHSQSLWLFLTNPKIPLTNNEAERRLRGFVIQRKISYGTTSDAGDKFRDRLHSLIETCKKRKISSLDTLSRI
ANAVVRQQPYPNVFDLKHTSFYLNT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1457 bp | 485 aa | 106 | 1562 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKKKSKFSSSPPIASDINEANALISELWEQLRHYEDRLQTSSANSSKSPSSDGPKERAERKKAQSSSGRHPRGAKQGHEGHKRQLSELKASDTVVDCYP
ESTCPCCGDADLKMSDKPFYRHQVHEIPAPRIDITEYRLYSGKCAQCGEAVKAQKPSDIPQGIMGPNLMSQIAILAGQYHLSVRKIRSLLQEQFGTTFSV
GAISEAQSRIASMLTPLHQAIKTNIQSAPLVHVDETSHSRNDEKGLRWCWLVASEDLVYEKILYSRSMHSAKTVLDSDYCGIVVSDQYSGYNWLSPDRHQ
LCWAHVIRNLQQIADYTGKGHTAKIGQRLVLLSKLVFRTRHRWESSQIDDALYLNRLNRIRCRFNHWLEKGATQIPIQCYQGRCRKLKEHSQSLWLFLTN
PKIPLTNNEAERRLRGFVIQRKISYGTTSDAGDKFRDRLHSLIETCKKRKISSLDTLSRIANAVVRQQPYPNVFDLKHTSFYLNT
ESTCPCCGDADLKMSDKPFYRHQVHEIPAPRIDITEYRLYSGKCAQCGEAVKAQKPSDIPQGIMGPNLMSQIAILAGQYHLSVRKIRSLLQEQFGTTFSV
GAISEAQSRIASMLTPLHQAIKTNIQSAPLVHVDETSHSRNDEKGLRWCWLVASEDLVYEKILYSRSMHSAKTVLDSDYCGIVVSDQYSGYNWLSPDRHQ
LCWAHVIRNLQQIADYTGKGHTAKIGQRLVLLSKLVFRTRHRWESSQIDDALYLNRLNRIRCRFNHWLEKGATQIPIQCYQGRCRKLKEHSQSLWLFLTN
PKIPLTNNEAERRLRGFVIQRKISYGTTSDAGDKFRDRLHSLIETCKKRKISSLDTLSRIANAVVRQQPYPNVFDLKHTSFYLNT
Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
orfAB of ISVch7 is 76% aa similar to ISPpr14.
orfAB of ISVch7 is 76% aa similar to ISPpr14.
References
1] Yamasaki,S., Shimizu,T., Hoshino,K., Ho,S.T., Shimada,T., Nair,G.B. and Takeda,Y. (1999) Gene 237 (2), 321-332.
2] Yamasaki,S., Shimizu,T. and Hoshino,K. (1998) Direct submission GenBank.
3]ISfinder annotation (2008).
2] Yamasaki,S., Shimizu,T. and Hoshino,K. (1998) Direct submission GenBank.
3]ISfinder annotation (2008).