ISfinder Mirror Site

ISBj7_B

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) ISBj13

Accession number	Transposition	Origin	Host
NC_004463	ND	Bradyrhizobium japonicum	Bradyrhizobium japonicum USDA 110

DNA section

IS Length : 2865 bp

Ends

IR Length : 40/50

IRL : GTAAGCGGTGTGTCTCCCACACCTTGTTTGGCGTGAGCTGATCGGCAACA
IRR : GTAAGCGGCGTGTCTCCCGCACCTTGTTGCTGTTGAGCAGACCGGCAATA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CACTCCGCGT	CTTAAATC	GCCGCATCCT	8

DNA sequence

GTAAGCGGTGTGTCTCCCACACCTTGTTTGGCGTGAGCTGATCGGCAACATGCGAGCCGTCATATACAGTAAGGTTCGCATATATGGTTGACCATATAGT
TGACGCTTCAGAGCCACGGGGCCGGGTGGATATCCAGGTCGGAGCGGGGCGTCGGCGGCGGTGGAGCGCCGTGGTCAAGGGGCGGATCGTGGCGGAGTCA
TATGCGCCGGGCACAGTTGTGTCCGAGGTGGCGCGCCGGCACGACCTCTCACCGCAACACCTGTTTGCTTGGCGCAAGGCCGCGCGTGCCGGTCTGCTGA
GCTTGCCGGCGGAGGATGCACCGCTCTTCGTTCCGGTGGTGTCGGAGCTTCGCCCCGCTGGGATGTGCGTGGAAGCGGCAACGCGGAACGGGATAACGAT
CAGCATCGAGATTGGCGATGCGGTAGTTCGCGCAGCGGCGGGGGTCGATCCGACCTGGCTGCGCGATGTGCTGCGGGCCGTGAGGGCGACGACATGATCG
CGGCTTCGGCGGGCGTGAAGATCATGGTCGCGACGCGGCCGGTCGACTTTCGTAAAGGTGCCGATGGTCTCGCCGCGCTGGTGCGCGAGCAACTCCACCA
TGATCCGTTCGCGGGAACGATTTTCGTCTTCCGTTCAAAGCGAGCGGACCGTTTGAAGATTTTTGCCTGGGACGGGTCCGGGCTCGTGCTGCTGTGGAAG
CGGCTGGAGCATAGCGCGTTCCGCTGGCCGCCGATCAGCGATGGCGTGATGCGTTTGTCTGCCTCACAGCTCGCCGCGCTGATGGACGGCCTGGATTGGT
CGCGCCTGCATGCCCGCGACATTGGTCAGCCGAGCGCAACGTCGTGAACCCTGTGACCAATTGAATCATGTCGGCTGCAGTCTTCCACAAGGGCCCGCGA
ATCAGCTTGAGTACCGGGCGTGAGCGAGACCACGGATCAACTTCCGACCGATATCGAGGCGCTGCAGGCGCTGGTTGCGGCGGCACGCGCGGAGCGCGAC
GCGGCGATCGCAAAGTGCGACGCGGCGATCATCGAACGCGATCAGGCGCTGTCGCAAATGGATCGCCTTCAGCATCTCTTGCGCCAGCTGCAACGCGCGC
AGTTCGGTCGCCGCTCGGAGAAGCTCGACCCTGAGCAGTTGCAACTGGCGATCGAGGACATCGAGCAGGCCATTGCCGGCGATGAAGCGGTCCAGGACAG
GAAGGACACGGCAGGCGCACGCCAGCGTGCTGAACGGCGCCGCGCCAGCCGTGGTGCGCTTCCGGCCCATCTGCCGCATATCGACGTCACGATTGCCCCG
GAGGATACCAACTGTCCGTGCTGCCGCACGCCTCTGCATGTGATCGGTGAGGAGACCTCACGGCGGCTCGACGTCGTCCCGGCGCAGTTCCGCGTGATCG
TCACCCATCGTCCCAAATATGCCTGTCGGGCCTGCACGGATGGCGTGGTTCAGGCGCCGGCACCAGAGCGGCTGATCAAGGGCGGCTTGCCGACCGAGGC
GATGGTCGCCCATGTTCTGGTCGCCAAATTTGCCTGGCACCTGCCGCTGTATCGGCAGGCTCAGATGCTGCTGGCGCAGGGTATCGACATCAAGCGCTCG
GTTTTGGCGTTCTGGGTGGGCTATGCAGGCGCAGAGCTTCGTCCGCTCTGGCTCAGGCTGCGCGAGATCATTTTGACCGCGAACAAGATCGCGGTGGACG
AGACCACAGCGCCTGTGCTCGATCCCGGCCGCGGCCGCACCAAGAAAGGCTTCTTCTGGGCGATCGCGCGCGACGATCGGCCGTGGGGCGGGGCCGATCC
GCCGGCAGTGGCCTACACTTATGCGCCTGGTCGCGGCGCCGTCCACGCGCTCAAGCTGCTCGACGGTTATCGCGGCATCGTGCAATGCGACGGCTACACC
GTCTACAAGACCATTGCCGACACCGCGCCTGACGCGGCGATCACGCTCGCCTTCTGCTGGGCTCATCTGCGGCGGCGCTTCTTCGACGTTGTCCAGGACG
GTCCTGCCCCGATCGCGAGCGAAGCGCTCGAGCGTATTGCCGCTCTCTATGCGATCGAGAAGACGATCAGGGGCCGCAGCGCCGATGAGCGTCGTGCCAT
CCGCCAGGAGCGCAGCAAGCCGCTGGTGCTGGCGCTCAAGGCCTGGTTCGAGCAGCAACTCACCCGCGTCTCGGGCAAAGCGACAATCGCCGAGCACATC
CGTTACGCCTTGAACCACTGGGTTGGCCTGATGCGCTTCCTTGACGACGGCCGCATCGAGCTCGACACAAATATTGTCGAGCGAAGCATCCGTCCTCTTA
TCCTCAACCGCAAGAACGCACTGTTCGCGGGGCATGATGAGGGCGCGGAGAACTGGGCCTGCATCGCTTCGCTGATTGAGACCTGCAAGCTCTGCGGCGT
GGAGCCGCAGGCCTATCTGACCGACGTCCTCACCAGACTCGTCAACCTCTGGCCGGCCTCGCGTCTGGACGAACTCATGCCCTGGGCGTGGAGCGCCGAG
CAAGCTAAAAGCCTCGCCGCTTGATGAACTCGCTTCCTGGATCGCGACCGCTCCAGCCGGCCTCGTCGCCACCGGCTCCGTGTGTTCCGACAACGTCAAC
AACCCGTCGCGCACCGCCTTGCGCCAGGTCGACAAATGCTGCGGCGATAGACCGTGGCGCCGCGCGACGTCCGAGACGACCGCGCCGGGAACAAAGGATT
CCGCGACGATCCGTCCCTTGACCTCGCCACCCCAGCGCCGCCGAACCCGTAGCTCCGATGCGTCGACAACACGCTCGATCATATATGCAAACCGTCCTCT
TCAAGACGGAACGCATATTGCCGGTCTGCTCAACAGCAACAAGGTGCGGGAGACACGCCGCTTAC

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
414 bp	137 aa	84	497	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MVDHIVDASEPRGRVDIQVGAGRRRRWSAVVKGRIVAESYAPGTVVSEVARRHDLSPQHLFAWRKAARAGLLSLPAEDAPLFVPVVSELRPAGMCVEAAT
RNGITISIEIGDAVVRAAAGVDPTWLRDVLRAVRATT

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
354 bp	117 aa	494	847	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIAASAGVKIMVATRPVDFRKGADGLAALVREQLHHDPFAGTIFVFRSKRADRLKIFAWDGSGLVLLWKRLEHSAFRWPPISDGVMRLSASQLAALMDGL
DWSRLHARDIGQPSATS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1605 bp	534 aa	920	2524	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VSETTDQLPTDIEALQALVAAARAERDAAIAKCDAAIIERDQALSQMDRLQHLLRQLQRAQFGRRSEKLDPEQLQLAIEDIEQAIAGDEAVQDRKDTAGA
RQRAERRRASRGALPAHLPHIDVTIAPEDTNCPCCRTPLHVIGEETSRRLDVVPAQFRVIVTHRPKYACRACTDGVVQAPAPERLIKGGLPTEAMVAHVL
VAKFAWHLPLYRQAQMLLAQGIDIKRSVLAFWVGYAGAELRPLWLRLREIILTANKIAVDETTAPVLDPGRGRTKKGFFWAIARDDRPWGGADPPAVAYT
YAPGRGAVHALKLLDGYRGIVQCDGYTVYKTIADTAPDAAITLAFCWAHLRRRFFDVVQDGPAPIASEALERIAALYAIEKTIRGRSADERRAIRQERSK
PLVLALKAWFEQQLTRVSGKATIAEHIRYALNHWVGLMRFLDDGRIELDTNIVERSIRPLILNRKNALFAGHDEGAENWACIASLIETCKLCGVEPQAYL
TDVLTRLVNLWPASRLDELMPWAWSAEQAKSLAA

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
291 bp	96 aa	2580	2870	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

VCSDNVNNPSRTALRQVDKCCGDRPWRRATSETTAPGTKDSATIRPLTSPPQRRRTRSSDASTTRSIIYANRPLQDGTHIAGLLNSNKVRETRRLP

Blast result :

Comments

ISBj7_B is 55% (ORFA) aa similar to ISApr6,
83% aa (ORFB) to ISFpe1, 71% aa (ORFC : the transposase) to ISBrsp6.
ORFD is a passenger gene, with an unknown function.

References

1] Kaneko,T., Nakamura,Y., Sato,S., Minamisawa,K., Uchiumi,T., Sasamoto,S., Watanabe,A., Idesawa,K., Iriguchi,M., Kawashima,K., Kohara,M., Matsumoto,M., Shimpo,S., Tsuruoka,H., Wada,T., Yamada,M. and Tabata,S. (2002) DNA Res. 9 (6), 225-256

2]Kaneko,T., Nakamura,Y., Sato,S., Minamisawa,K., Uchiumi,T., Sasamoto,S., Watanabe,A., Idesawa,K., Iriguchi,M., Kawashima,K., Kohara,M., Matsumoto,M., Shimpo,S., Tsuruoka,H., Wada,T., Yamada,M. and Tabata,S.(2002)DNA Res. 9 (6), 189-197

3]Kaneko,T. (2002) Direct submission GenBank.

4] ISfinder annotation (2008).