ISGdi11
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Gluconacetobacter diazotrophicus | Gluconacetobacter diazotrophicus PAl 5 |
DNA section
IS Length : 1200 bp
Ends
IR Length : 34/36
IRL : TGACCTTGCCCCCTGAAATGCCCTCGCATTGAAGTAAGGTCTGTCCACTG
IRR : TGACCCTGCCCCGAACGTGCCCTCAGTTTTGAGTTAGGGTCTGTGTATTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTGCTTGGAC | GCGG | GCATTGGGAC | 4 |
| AAATAGCTCG | CGAA | CCCTCGGCGC | 4 |
| ACCCAGGCTC | GACG | CGCCGCAGGC | 4 |
| AGGTGTGGTG | TAGG | AGCACCGTTC | 4 |
| ATTGATGGAG | ATGG | TGATGGCGCG | 4 |
| GAACTCACCC | CGCTCCACCT | 0 | |
| AGTTCTACGG | CACCTAATGG | 0 | |
| GTCGCCCTGG | AGCCGCGACG | 0 | |
| AGTTCTACGG | CGCGCTTTCT | 0 | |
| CGGCCGATTG | TCGG | ATGTTCAGGA | 4 |
| TTTCACAAGC | GTTA | CATACCACAA | 4 |
| GAACGTTGTG | GTAC | CTGTCCCGCC | 4 |
| GGGCTGGGCC | CGCTCCACCT | 0 | |
| GTTGATGTCC | GACCATCTGT | 0 |
DNA sequence
TGACCTTGCCCCCTGAAATGCCCTCGCATTGAAGTAAGGTCTGTCCACTGGAGACAGACGATGAAGAAAGCGCGATTTACGCAGGACCAGATTATCGGGG
TCCTGAAAGAGCATCAGGCGGGCGCTACGGCTGCGGATCTGTGCCGCAAGCACGGGATCAGTGACGCGACCTTCTACACCTGGCGGTCGAAATACGGCGG
GATGGAGGTGTCGGAAGCGCGGCGCCTCAAGGCTCTTGAAGAAGAGAACGCGAAGCTGAAGCGGCTTCTGGCGGAGAGCGTGATGGACGTCTCGACGCTG
AAGGAACTACTGGCAAAAAACTCGTGACGCCCGGTTTGCGGCGGGAAGCCGTGACCTGGGCGATCCGGGAGAAAGAGTATTCGCAGCGACGGGCCTGCCG
GCTGATCGGCATGGACCCGAAGACCTGGCGCTATGCGTCACGCCGCCCGGATGATGCCGCAGCGCGCGGGCGGCTGCGCGAACTGGCTGGGGAGCGACGG
CGATTTGGCTACCGGCGACTGCATATCCTGCTCGGCCGGGAAGGAATGACGATGAACCACAAGAAGCTGTTCCGGCTGTATCGCGAAGAGGGGCTGTCGG
TCCGCAAGCGTGGCGGCCGGAAACGGGCGCTGGGCACGCGCTCGCCGATGATGCTGCCCGACGGGCCGAACCAGCGCTGGAGCCTGGATTTCGTCTCGGA
TGCATTGAACAACGGACGGCGCTTCCGGGTGCTGACGGTGGTCGACGACTACACGCGCGAATGTCTGGCGCTGGTGGCGGACACCTCGTTATCAGGCGAA
CGCCTCGGTCGTGAACTCGACCGGATCGGCGAGCATCGCGGCTGGCCGCTGATGATCGTTAGCGACAATGGCACCGAGATGACATCGAACGCGATCCTGG
CCTGGCAGCAGAAGCGATCGGTGCTGTGGCACTATATCGCACCGGGCAAGCCGCAGCAGAACGGGTTCGTCGAGAGCTTCAACGGCCGGTTCCGCGACGA
ATGCCTCAATGAGCATCTGTTCCGTAACATCGCCCACGCTCGGACGGTCATCGAGGACTGGCGGGCCGACTACAACGCCGTCAGGCCTCACACCAGCCTC
AATGGCATGACGCCAGAGGCTTTCGCTCAACACGCCACCAAGGCATACAACAATACACAGACCCTAACTCAAAACTGAGGGCACGTTCGGGGCAGGGTCA
TCCTGAAAGAGCATCAGGCGGGCGCTACGGCTGCGGATCTGTGCCGCAAGCACGGGATCAGTGACGCGACCTTCTACACCTGGCGGTCGAAATACGGCGG
GATGGAGGTGTCGGAAGCGCGGCGCCTCAAGGCTCTTGAAGAAGAGAACGCGAAGCTGAAGCGGCTTCTGGCGGAGAGCGTGATGGACGTCTCGACGCTG
AAGGAACTACTGGCAAAAAACTCGTGACGCCCGGTTTGCGGCGGGAAGCCGTGACCTGGGCGATCCGGGAGAAAGAGTATTCGCAGCGACGGGCCTGCCG
GCTGATCGGCATGGACCCGAAGACCTGGCGCTATGCGTCACGCCGCCCGGATGATGCCGCAGCGCGCGGGCGGCTGCGCGAACTGGCTGGGGAGCGACGG
CGATTTGGCTACCGGCGACTGCATATCCTGCTCGGCCGGGAAGGAATGACGATGAACCACAAGAAGCTGTTCCGGCTGTATCGCGAAGAGGGGCTGTCGG
TCCGCAAGCGTGGCGGCCGGAAACGGGCGCTGGGCACGCGCTCGCCGATGATGCTGCCCGACGGGCCGAACCAGCGCTGGAGCCTGGATTTCGTCTCGGA
TGCATTGAACAACGGACGGCGCTTCCGGGTGCTGACGGTGGTCGACGACTACACGCGCGAATGTCTGGCGCTGGTGGCGGACACCTCGTTATCAGGCGAA
CGCCTCGGTCGTGAACTCGACCGGATCGGCGAGCATCGCGGCTGGCCGCTGATGATCGTTAGCGACAATGGCACCGAGATGACATCGAACGCGATCCTGG
CCTGGCAGCAGAAGCGATCGGTGCTGTGGCACTATATCGCACCGGGCAAGCCGCAGCAGAACGGGTTCGTCGAGAGCTTCAACGGCCGGTTCCGCGACGA
ATGCCTCAATGAGCATCTGTTCCGTAACATCGCCCACGCTCGGACGGTCATCGAGGACTGGCGGGCCGACTACAACGCCGTCAGGCCTCACACCAGCCTC
AATGGCATGACGCCAGAGGCTTTCGCTCAACACGCCACCAAGGCATACAACAATACACAGACCCTAACTCAAAACTGAGGGCACGTTCGGGGCAGGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 61 | 327 | + | No |
Description : First part of the transposase
ORF sequence :
MKKARFTQDQIIGVLKEHQAGATAADLCRKHGISDATFYTWRSKYGGMEVSEARRLKALEEENAKLKRLLAESVMDVSTLKELLAKNS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 939 bp | 312 aa | 241 | 1179 | + | No |
Description : Second part of the transposase
ORF sequence :
RREREAEAASGGERDGRLDAEGTTGKKLVTPGLRREAVTWAIREKEYSQRRACRLIGMDPKTWRYASRRPDDAAARGRLRELAGERRRFGYRRLHILLGR
EGMTMNHKKLFRLYREEGLSVRKRGGRKRALGTRSPMMLPDGPNQRWSLDFVSDALNNGRRFRVLTVVDDYTRECLALVADTSLSGERLGRELDRIGEHR
GWPLMIVSDNGTEMTSNAILAWQQKRSVLWHYIAPGKPQQNGFVESFNGRFRDECLNEHLFRNIAHARTVIEDWRADYNAVRPHTSLNGMTPEAFAQHAT
KAYNNTQTLTQN
EGMTMNHKKLFRLYREEGLSVRKRGGRKRALGTRSPMMLPDGPNQRWSLDFVSDALNNGRRFRVLTVVDDYTRECLALVADTSLSGERLGRELDRIGEHR
GWPLMIVSDNGTEMTSNAILAWQQKRSVLWHYIAPGKPQQNGFVESFNGRFRDECLNEHLFRNIAHARTVIEDWRADYNAVRPHTSLNGMTPEAFAQHAT
KAYNNTQTLTQN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1119 bp | 372 aa | 61 | 1179 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKARFTQDQIIGVLKEHQAGATAADLCRKHGISDATFYTWRSKYGGMEVSEARRLKALEEENAKLKRLLAESVMDVSTLKELLAKKLVTPGLRREAVTW
AIREKEYSQRRACRLIGMDPKTWRYASRRPDDAAARGRLRELAGERRRFGYRRLHILLGREGMTMNHKKLFRLYREEGLSVRKRGGRKRALGTRSPMMLP
DGPNQRWSLDFVSDALNNGRRFRVLTVVDDYTRECLALVADTSLSGERLGRELDRIGEHRGWPLMIVSDNGTEMTSNAILAWQQKRSVLWHYIAPGKPQQ
NGFVESFNGRFRDECLNEHLFRNIAHARTVIEDWRADYNAVRPHTSLNGMTPEAFAQHATKAYNNTQTLTQN
AIREKEYSQRRACRLIGMDPKTWRYASRRPDDAAARGRLRELAGERRRFGYRRLHILLGREGMTMNHKKLFRLYREEGLSVRKRGGRKRALGTRSPMMLP
DGPNQRWSLDFVSDALNNGRRFRVLTVVDDYTRECLALVADTSLSGERLGRELDRIGEHRGWPLMIVSDNGTEMTSNAILAWQQKRSVLWHYIAPGKPQQ
NGFVESFNGRFRDECLNEHLFRNIAHARTVIEDWRADYNAVRPHTSLNGMTPEAFAQHATKAYNNTQTLTQN
Blast result :
Comments
ISGdi11 is 82% aa similar to ISRde2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Miriam Land (2008) Direct submission.