ISAzca2

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009937 ND Azorhizobium caulinodans
Azorhizobium caulinodans ORS 571
DNA section
IS Length : 2458 bp

Ends


IR Length : 19/25

IRL : GTAAGCGTCGTCCTACCCCCACCTTTCGCCGGCGAGGCTGAGGGGGCGAT
IRR : GTAAGCGTCGTCTTCAGGCCACGTTGCTGAGGACCGGCTGTGCTGGGTAG

Insertion site


Left flankDirect repeatRight flankDR Length
GTCACAAACGGCTTTTAGGGGG2

DNA sequence

GTAAGCGTCGTCCTACCCCCACCTTTCGCCGGCGAGGCTGAGGGGGCGATGTTCCGAGCCTGGAGGGCTTGGACATATGACGATTTCAGAGCTTACGCTT
AAATCTAGCGCCGAGGAGCCGGTGCGGCGGTTCGAGGTTTTCACCGGAGCCGGGCGTCGCCGAGAGTGGGCTCCCGAGGAGAAGGCGCGGATTGTCACCG
AGAGCTTTGAGCCTGGAGCCACGGTGAGTGCAGTGGCGCGCCGCCACGGACTGTCGCCGCAGCAGTTGTTCACGTGGCGCCGTGAGGCGCGGAAAGCGAG
CGAGACGATCCCGGCTTTCGTGCCGGCGGTGGTCGCTCCGGAACCGGTTCCCGCATCTGAGCCGTCCGCCCAACCGTCACGGCCGAAGCCGCGTGATCGG
CAACGTCGCGCGGCAGCCATCGAGGTCGACGTCGCCGGGGTCAGGGTGACGATTGAGAACGGGGCCTCGCCCGCCACCATCGCAGCGGTGCTCGGCGCGC
TGAAGGCCGGATCGTGATCGGCCCGAGCGGCGCGGTCCGGGTCATGGTGGCGACACGGCCGGTGGACTTCCGCAAGGGGGCGGAGGGTCTCGCCGCTTTG
GTGCGCGAGACCATGGGCGCCGATCCGTTCTCCGGCACCGTGTACGTGTTCCGGGCCAAGCGAGCGGACCGGGTGAAGCTGGTGTTCTGGGACGGCACCG
GCGTCGTGCTGGTGGCGAAGCGGCTTGAGGACGGGGAGTTCCGCTGGCCGAAGATCGAGGACGGCACCCTGCAGCTGACGTCGGCCCAGCTCCAGGCCCT
GCTCGAGGGCCTCGACTGGCGGCGAGTGCATGAGGCGCAGCGCACGATGACACCGGTCACCGCCAGCTGAAGAGGCTCGCAGGGGCCTGGCGAATGTGAT
TCACTTCCGGTCATGGCCTCGCTCTCCACCGAGCCCCCCCGTGACCCCGAGACGCTCCAGGCGATGCTGATCGCGCGGGACACGGAGATCGAGCGCCTGC
GCCAGATCATCAAGGAGTTGCAGCGCCACCGCTTCGGACGGCGGGCGGAGAGCCTGCCCGAGGACCAACTGCTGCTGGCGCTCGAAGAGGCCGAACAGGT
CGAGGCTGCCTACTTCGCCGCGTCCGATGAACAGGTGCCGGCCGAGAAGGCCGAGCGGGTGGCCAAACGCCGTGCGAACCGCGGTGCTTTGCCCGCCCAT
CTGCCGCGGATCGAGACCGTGATCGATATCGAGAGTGACATCTGCCCCTGCTGTTCCGGCAAGCTGCACCGCATCGGCGAGGATGTGGCGGAACGGCTCG
ACATGGTGCCTGCCCAGTTCCGCGTCCTGGTGGTGCGGCGGCCGAAATATGCCTGCCGGTCCTGCGCGGACGTGGTGGTGCAGGCCCCCGCTTCGGCTCG
GTTGATCGAAGGTGGCATCCCGACCGAGGCAACCGTCGCCCATGTGCTGGTCTCGAAATACGCCGACCATCTGCCCCTGTATCGCCAAGTCCAGATCTAT
GCGCGGCAGGGCATCGCGCTCGACCGCTCGACGCTGGCGGACTGGGTCGGGCGCGCCGCCTTCCTGCTGCGACCTGTCCACGAGCGGCTGCTGGCGATGC
TAAGGGCCTCGACCAAGCTGTTCGCCGACGAGACCACGGCGCCGGTGCTCGATCCCGGCCGTGGCCGCACCAAGACCGGGCAGCTCTTCGCCTATGCCCG
TGATGACCGTCCGTGGGGCGGAGCCGATCCGCCCGCCGTCGCTTATGTCTATGCCCCCGATCGTAAGAGCGAGCGGCCGGTCGCCCACCTCGACGGCTTC
AGCGGCATCCTGCAGGTCGATGGCTATGGCGGCTACAAGGCGCTAGCCGGACGCAACGCCGTGAATCTGGCGTTCTGCTGGGCCCACGTGCGCCGCCGCT
TCTACGAGCTCGCTCAGAGCGGTCCCGCGCCGATCGCCAGCGAGGCCCTGCAGCGCATCGCCGAGCTCTATCGCCTCGAGGCTGCGATCCACGGCCGAAC
CCCAGATGAGCGCCGAGCCTCGCGCCAAGAGAAGAGCAGGCCGATCATCGAGGCCATGGAGCCATGGCTGCGCGAGAAGCTGGCTCTGATCAGCCAGAAG
ACCAAGCTGGCCGAGGCGATCCGCTATGCGCTCTCGCGCTGGCAGGGGCTGACCCCGTTCCTCGACGACGGCCGCGTCGAGTTGGACAACAACATCGTCG
AGCGCTCCATCCGACCCCTGGCCCTGACCCGGAAGAATGCGCTCTTCGCCGGCTCCGACGGCGGCGCCGAGCACTGGGCTGTCATGGCTTCGCGGGTCGA
GACCTGCAAGCTCAACGACATCGACCCCCAGGCCTACCTCGCGGACGTCATCACCCGCATCGTCAACAGCCACCCCAACAGCCGCATCGACGACCTCATG
CCATGGGCCTACCCAGCACAGCCGGTCCTCAGCAACGTGGCCTGAAGACGACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
441 bp146 aa77517+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MTISELTLKSSAEEPVRRFEVFTGAGRRREWAPEEKARIVTESFEPGATVSAVARRHGLSPQQLFTWRREARKASETIPAFVPAVVAPEPVPASEPSAQP
SRPKPRDRQRRAAAIEVDVAGVRVTIENGASPATIAAVLGALKAGS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
357 bp118 aa514870+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

VIGPSGAVRVMVATRPVDFRKGAEGLAALVRETMGADPFSGTVYVFRAKRADRVKLVFWDGTGVVLVAKRLEDGEFRWPKIEDGTLQLTSAQLQALLEGL
DWRRVHEAQRTMTPVTAS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1533 bp510 aa9132445+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MASLSTEPPRDPETLQAMLIARDTEIERLRQIIKELQRHRFGRRAESLPEDQLLLALEEAEQVEAAYFAASDEQVPAEKAERVAKRRANRGALPAHLPRI
ETVIDIESDICPCCSGKLHRIGEDVAERLDMVPAQFRVLVVRRPKYACRSCADVVVQAPASARLIEGGIPTEATVAHVLVSKYADHLPLYRQVQIYARQG
IALDRSTLADWVGRAAFLLRPVHERLLAMLRASTKLFADETTAPVLDPGRGRTKTGQLFAYARDDRPWGGADPPAVAYVYAPDRKSERPVAHLDGFSGIL
QVDGYGGYKALAGRNAVNLAFCWAHVRRRFYELAQSGPAPIASEALQRIAELYRLEAAIHGRTPDERRASRQEKSRPIIEAMEPWLREKLALISQKTKLA
EAIRYALSRWQGLTPFLDDGRVELDNNIVERSIRPLALTRKNALFAGSDGGAEHWAVMASRVETCKLNDIDPQAYLADVITRIVNSHPNSRIDDLMPWAY
PAQPVLSNVA

 

Blast result :
Comments
orfA, orfB and orfC of ISAzca2 are respectively 62%, 87% and 82% aa similar to orfA, orfB and orfC of ISFpe1.
References
1] Suzuki,S., Aono,T., Lee,K.B., Suzuki,T., Liu,C.T., Miwa,H., Wakao,S., Iki,T. and Oyaizu,H. (2007) Appl. Environ. Microbiol. 73 (20), 6650-6659.
2] Lee,K.B., Aono,T., Kaneko,T. and Oyaizu,H. (2007) Direct Submission GenBank.
3] ISfinder annotation (2008)