ISSba19
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Shewanella baltica | Shewanella baltica OS233 |
DNA section
IS Length : 1373 bp
Ends
IR Length : 15/23
IRL : taatggaATGGACCCATCCACTTTTAATCGGCCTCACAGTGGGCCACGAT
IRR : ----tatATGGACCCATCCGGTTTGCAAGACATTCGATAGCGATTTTGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCTCAAGGA | TGCCGATGGTC | 0 | |
| GCTCAAGGA | TGCCGATGGTC | 0 | |
| AAGCATGGA | TGCCGAGCTGG | 0 | |
| CGACATGGA | TGCCGCCGTCG | 0 | |
| GGTCATGGA | TGGCAAGCTAG | 0 | |
| AGGCATGGA | TGCCGAGATAG | 0 | |
| CGCCATGGA | TGGCAAGCTAG | 0 |
DNA sequence
TAATGGAATGGACCCATCCACTTTTAATCGGCCTCACAGTGGGCCACGATGTAGTCGCTAAAACTCATCAGAAAGAGGACGGGTCCACTTATGAAGATTA
CTACAATTGGTTTAGATATCGCAAAGTCTGTTTTTCATGCTGTTGGTGTCGATAAAGCAGGCAAGTTAATCAAAAAGAAAATGCTTAGACGCAAGGACTT
ACTTCCCTTTCTTGCTCAAATTGAGCCGTGTCTGATTGTGATGGAAGCCTGTAGTGGGGCAAATTATTGGGCCAGAGAATTTGAGCTGTTGGGCCATAAC
GTCAAGCTAATCGCACCTCAATTTGTGGTGCCTTTTCGGCAAGGTAATAAAAATGACTACAACGATGCGCTAGCCATCGCTGAAGCTGCGCAGCGACCCA
ACATGCGGTTTGTAAAACCCAAAAGCGTTGAGCAGCAAGATGTGCAACTGCTCCACCGCATGCGCGAACGATTAACTAAACAATCAACCGCCTTAATCAA
TCAAGTCAGAGGGATGTTAGCGGAATACGGCATTGTGATAACCAAAAGTAAATCGGCTTTTAAAGTACAGTTCCCAGATATTTTAGCGGATGAAACCAAT
GCGCTGACTACCAAAGGTCGGGCCATTTTTTATCAGTTATATGAAGAGTTTAGTGATATAGAAAAACGGCTTAAGGGTTGTGACGTTCAAGTGCTCACTG
AAACTAAGAATAATGTGATTTGCCAGCGTTTAGAAACTATCCCTGGTATTGGCCCAGTCACCGCTACCGCCTTTTATGCGGCCGTCGGTGATGGTAAAGA
TTTCAGTAATGGTAGACACTTTTCAGCTTGGTGTGGCTTGGTTCCCAAGCAACACAGCAGTGGAGGTAAAGATAACCTCCTTGGAATAAGCAAGCGGGGA
AATCCTTACTTACGTACCCTGTTCATCCACGGCGCCAGAGCTGTGTTACAGCACAGTTGTCATAAACAGGATAAATTTAGTTGCTGGGCAATCCAGTTAG
CAGAACGCCGAGGTTTTAACCGAGCCTGTGTAGCCGTCGCCAATAAACTCGCGAGAATGGCATGGGTTATCGCGGCGCATGACGAAGAATATCGACTTCC
AGTATAGTGCTGAATAGATTCAATAAACGTTAATAATTAACCAACCAGCAAGTTGCTAAGACACTTGAATTGATGATGAGACAGTCAGACTGATCTACTG
AAAACCTTAGCTCGGCATAGGCTCATAATGAAGCCGCAAGGATGATAAGGACAGTAGAAGCAGATATCCATCAGGGCCAGAGGCATACCTCAATAACAGG
CCGAATATATGGGTGCAATGAACTCTTCTCAAAATCGCTATCGAATGTCTTGCAAACCGGATGGGTCCATATA
CTACAATTGGTTTAGATATCGCAAAGTCTGTTTTTCATGCTGTTGGTGTCGATAAAGCAGGCAAGTTAATCAAAAAGAAAATGCTTAGACGCAAGGACTT
ACTTCCCTTTCTTGCTCAAATTGAGCCGTGTCTGATTGTGATGGAAGCCTGTAGTGGGGCAAATTATTGGGCCAGAGAATTTGAGCTGTTGGGCCATAAC
GTCAAGCTAATCGCACCTCAATTTGTGGTGCCTTTTCGGCAAGGTAATAAAAATGACTACAACGATGCGCTAGCCATCGCTGAAGCTGCGCAGCGACCCA
ACATGCGGTTTGTAAAACCCAAAAGCGTTGAGCAGCAAGATGTGCAACTGCTCCACCGCATGCGCGAACGATTAACTAAACAATCAACCGCCTTAATCAA
TCAAGTCAGAGGGATGTTAGCGGAATACGGCATTGTGATAACCAAAAGTAAATCGGCTTTTAAAGTACAGTTCCCAGATATTTTAGCGGATGAAACCAAT
GCGCTGACTACCAAAGGTCGGGCCATTTTTTATCAGTTATATGAAGAGTTTAGTGATATAGAAAAACGGCTTAAGGGTTGTGACGTTCAAGTGCTCACTG
AAACTAAGAATAATGTGATTTGCCAGCGTTTAGAAACTATCCCTGGTATTGGCCCAGTCACCGCTACCGCCTTTTATGCGGCCGTCGGTGATGGTAAAGA
TTTCAGTAATGGTAGACACTTTTCAGCTTGGTGTGGCTTGGTTCCCAAGCAACACAGCAGTGGAGGTAAAGATAACCTCCTTGGAATAAGCAAGCGGGGA
AATCCTTACTTACGTACCCTGTTCATCCACGGCGCCAGAGCTGTGTTACAGCACAGTTGTCATAAACAGGATAAATTTAGTTGCTGGGCAATCCAGTTAG
CAGAACGCCGAGGTTTTAACCGAGCCTGTGTAGCCGTCGCCAATAAACTCGCGAGAATGGCATGGGTTATCGCGGCGCATGACGAAGAATATCGACTTCC
AGTATAGTGCTGAATAGATTCAATAAACGTTAATAATTAACCAACCAGCAAGTTGCTAAGACACTTGAATTGATGATGAGACAGTCAGACTGATCTACTG
AAAACCTTAGCTCGGCATAGGCTCATAATGAAGCCGCAAGGATGATAAGGACAGTAGAAGCAGATATCCATCAGGGCCAGAGGCATACCTCAATAACAGG
CCGAATATATGGGTGCAATGAACTCTTCTCAAAATCGCTATCGAATGTCTTGCAAACCGGATGGGTCCATATA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1017 bp | 338 aa | 91 | 1107 | + | No |
Chemistry : DEDD
ORF sequence :
MKITTIGLDIAKSVFHAVGVDKAGKLIKKKMLRRKDLLPFLAQIEPCLIVMEACSGANYWAREFELLGHNVKLIAPQFVVPFRQGNKNDYNDALAIAEAA
QRPNMRFVKPKSVEQQDVQLLHRMRERLTKQSTALINQVRGMLAEYGIVITKSKSAFKVQFPDILADETNALTTKGRAIFYQLYEEFSDIEKRLKGCDVQ
VLTETKNNVICQRLETIPGIGPVTATAFYAAVGDGKDFSNGRHFSAWCGLVPKQHSSGGKDNLLGISKRGNPYLRTLFIHGARAVLQHSCHKQDKFSCWA
IQLAERRGFNRACVAVANKLARMAWVIAAHDEEYRLPV
QRPNMRFVKPKSVEQQDVQLLHRMRERLTKQSTALINQVRGMLAEYGIVITKSKSAFKVQFPDILADETNALTTKGRAIFYQLYEEFSDIEKRLKGCDVQ
VLTETKNNVICQRLETIPGIGPVTATAFYAAVGDGKDFSNGRHFSAWCGLVPKQHSSGGKDNLLGISKRGNPYLRTLFIHGARAVLQHSCHKQDKFSCWA
IQLAERRGFNRACVAVANKLARMAWVIAAHDEEYRLPV
Blast result :
Comments
There are 6 copies of ISSba19 inserted in the S. baltica OS233 chromosome.
One copy interrupts a gene that is conserved in Shewanella. Comparative analysis of the interrupted gene with intact genes was used to identify the precise ends of ISSba19. There is extensive conservation among the sequences flanking the insertions suggesting target specificity. The transposase encoded by ISSba19 has 92% identity to ISSod19. The terminal inverted repeats start at position 8 from the 5' end and 4 from the 3' end of ISSba19.
One copy interrupts a gene that is conserved in Shewanella. Comparative analysis of the interrupted gene with intact genes was used to identify the precise ends of ISSba19. There is extensive conservation among the sequences flanking the insertions suggesting target specificity. The transposase encoded by ISSba19 has 92% identity to ISSod19. The terminal inverted repeats start at position 8 from the 5' end and 4 from the 3' end of ISSba19.
References
1] Romine, M. (2008) Direct submission.