ISKpn14
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000649 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae subsp pneumoniae MGH78578 plasmid pKPN4 |
DNA section
IS Length : 768 bp
Ends
IR Length : 18/23
IRL : GGTGATGCTACCAACTTGCTGATTTAGTGTATAATGGTGTTTTTGAGGTG
IRR : GGTAATGACTCCAATTTACTGATAGTGTTTTATGTTCAGATAGTGCCCGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGTATCAGTTTGGCGTGCG | GGCTTCGAGAAGCGCGAG | 0 |
DNA sequence
GGTGATGCTACCAACTTGCTGATTTAGTGTATAATGGTGTTTTTGAGGTGCTCCCGTGGCTTCAGTCTCCGTCTGCTGTCCCTCCTGTTCCGCTACTGAA
GGCGTGATGCGTAATGGCAAAAGTACTGCCGGGCATCAACGTTATCTCTGCTCTCACTGCCGTAAAACATGGCAGCTCACCTTCACTTATGCCGCTTCTC
AGCCCGGTACACACCAGAAAATCATTGATATGGCTATGAACGGCGTCGGTTGCCGTGCCACCGCACGACTAATGGGCGTGGGCCTCAACACCATTTTGCG
CCATTTAAAAAACTCAGGCCGCAGTCAGTAACCTCCCGGATACAGCCGGGCAGTGATGTCATTGTCTGTGCGGAGATGGACGAGCAATGGGGCTACGTCG
GTGCAAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGCAGGGCGGTGGTAGCCCACGTTTTCGGTGAACGCACTATGGCCACACTGGA
GCGTCTTCTGAGCCTGCTGTCAGTTTTTGACGTGGTGGTATGGATGACGGACGGCTGGCCGATGTATGAGTCACGTCTGAAGGGAAAACTGCACGTCATC
AGTAAGCGTTACACGCAGCGAATTGAGCGACACAACCTGAATCTGAGACAGCATCTGGCAAGGCTGGGCAGGAAGTCCCTGTCGTTCTCAAAATCGGTGG
AGCTGCATGACAAAGTCATCGGGCACTATCTGAACATAAAACACTATCAGTAAATTGGAGTCATTACC
GGCGTGATGCGTAATGGCAAAAGTACTGCCGGGCATCAACGTTATCTCTGCTCTCACTGCCGTAAAACATGGCAGCTCACCTTCACTTATGCCGCTTCTC
AGCCCGGTACACACCAGAAAATCATTGATATGGCTATGAACGGCGTCGGTTGCCGTGCCACCGCACGACTAATGGGCGTGGGCCTCAACACCATTTTGCG
CCATTTAAAAAACTCAGGCCGCAGTCAGTAACCTCCCGGATACAGCCGGGCAGTGATGTCATTGTCTGTGCGGAGATGGACGAGCAATGGGGCTACGTCG
GTGCAAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGCAGGGCGGTGGTAGCCCACGTTTTCGGTGAACGCACTATGGCCACACTGGA
GCGTCTTCTGAGCCTGCTGTCAGTTTTTGACGTGGTGGTATGGATGACGGACGGCTGGCCGATGTATGAGTCACGTCTGAAGGGAAAACTGCACGTCATC
AGTAAGCGTTACACGCAGCGAATTGAGCGACACAACCTGAATCTGAGACAGCATCTGGCAAGGCTGGGCAGGAAGTCCCTGTCGTTCTCAAAATCGGTGG
AGCTGCATGACAAAGTCATCGGGCACTATCTGAACATAAAACACTATCAGTAAATTGGAGTCATTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 276 bp | 91 aa | 56 | 331 | + | No |
Description : First part of the transposase
ORF sequence :
VASVSVCCPSCSATEGVMRNGKSTAGHQRYLCSHCRKTWQLTFTYAASQPGTHQKIIDMAMNGVGCRATARLMGVGLNTILRHLKNSGRSQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 525 bp | 174 aa | 229 | 753 | + | No |
Description : Second part of the transposase
ORF sequence :
YGYERRRLPCHRTTNGRGPQHHFAPFKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVF
DVVVWMTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
DVVVWMTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 698 bp | 274 aa | 56 | 753 | + | Yes |
Chemistry : DDE
ORF sequence :
VASVSVCCPSCSATEGVMRNGKSTAGHQRYLCSHCRKTWQLTFTYAASQPGTHQKIIDMAMNGVGCRATARLMGVGLNTILRHLKNSGRSQPGTHQKIID
MAMNGVGCRATARLMGVGLNTILRHLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVF
DVVVWMTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
MAMNGVGCRATARLMGVGLNTILRHLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVF
DVVVWMTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
Blast result :
Comments
ISKPn14 is 89% identical to IS1X2. Orf1= InsA: 91 aa; Orf2 = InsB ; Orf3= InsAB':274 aa,received by a -1 translational frameshift
References