ISfinder Mirror Site

ISFpe1

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AATP00000000	ND	Fulvimarina pelagi	Fulvimarina pelagi HTCC2506

DNA section

IS Length : 2489 bp

Ends

IR Length : 21/30

IRL : GTAAGCGTTGCCGTCACCCCACCTTGCTGATTGCGGCGTAGCCAGCGAGT
IRR : GTAAGCGTCATCCTCAGGCCACCGCGGTGAGATCGACTTTCGGCTTGAAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCTATAGAGG	GAACAGAT	TGACCTCCCT	8

DNA sequence

GTAAGCGTTGCCGTCACCCCACCTTGCTGATTGCGGCGTAGCCAGCGAGTTTCCGAGCCTTTCATGCGAGGTAGAGGCTTGGAGCTATGTCGATGTCATG
TGCTGATGCTGGCACTAAGCCAGTTCGGCGGTTCGAGGTCTTCACGGGCGCAGGACGGCGGCGCGACTGGAGCGACGAAGAGAAGGCGGCGATCGTCGCC
GAGAGCTATTCCGGCTTGGAAAGCGTTTGTGCCGTTGCCCGGCGTCACGGACTGAGCCATTCGCAACTCTTCACCTGGCGGCGCAAGTTGCGAGAGACGG
CTCTTGTGAAGTCCGATGCGACCGCGACCGAACCGGCATTCGTGCCGGCAATCGTCGAGCCTTCCATCATCGAGAAGAAGCCTCGCGCGAAGCGGAAACG
GTCACGCTCGTCCGGCAAGGCGATCCCGGCGGTCGAACTGGAGATCGACGGCGTCGCGGTGAAGATCGCCAAGGGTGCCGACGCAAAAACGATCGCGGCA
GTGATCGACGCATTGAAGGGAAGCGCGTGATCGGGCCAAGCGGCGCCGTTCGCGTTATGGTCGCGACGAAGCCCGTCGACTTCCGCAAAGGCGCCGAGGG
TCTCGCCGCCCTTGTGCGCGAGACGATGGCGGCCGATCCCTTTGGCGGTACGATCTACGTCTTCCGAGCCAAACGCGCCGACCGGATCAAGCTGATCTTC
TGGGACGGCACCGGTCTGGTTCTCGTTGCAAAGCGGCTCGAGGACGGCGCGTTCAAATGGCCTGCGATCACGGACGGCGTCATTCGGCTGACAGCCGCAC
AGCTCTCCGCGCTTCTCGAAGGTCTGGACTGGCGCCGCGTTCACGAGGCCCGAACGACACGGGTGCCCGAAGCTGTGAGCTGATCGGCATCGACGCCGAT
AAATCTCTCGCCGAAGGCGCGAGATTATGATTCTCTTCGGTTCATGGCCACTGCTCTCGAAGCACTTCCCGACGATCCCGGCACGCTGAAGGCGATGCTG
ATCGCCGAGCGCGTGCGGTCCGAGCGTCTTGAGCAGATCATCAAGGAATTGCAGCGCCATCGCTTCGGCCGCCGGGCCGAGACGCTGCCCGAGGACCAGC
TTCTTCTCGGTCTCGAAGACGTCGAGCAAGGCGTTGCCGTCGAAGAGGCCGAGGCTGAAAGCGCCGATCCAGAACGAAAGACCGAACGGGCGGCGAAGCG
CCGGGCGAACCGCGGTTCTCTGCCGGCCCATCTGCCGCGGATCGAGACCGTCGTCGACATCGAGGACAAGTCCTGTCCGTGCTGCCGGCATTGGCTCCAC
GTCATCGGCGAGGATGTCGCCGAGCGGCTCGACATCGTGCCCGCCCAGTTCCGTGTTCTTGTGACAAGGCGGCCGCGCTACGGCTGCCGGGCCTGCGAGG
GCGTCGTCGTTCAGGCACCTGCACCGAACAGATTGATCGAGGGCGGCATTCCGACCGAGGCGACGGTAGCGCAGGTCCTGGTCTCCAAATACGCCGATCA
CCTGCCGCTCTATCGTCAGGCGCAGATCTATGCCCGCCAGGGCGTGCGGCTCGACCGATCGACGCTCGCGGATTGGGTTGGACGGGCGGCCTTCCTGCTC
AGGCCTATCCACGAGCGGCTTCTCGGCCACTTGAAGGCGTCCACCAAGCTCTTCGCCGACGAAACGACTGCGCCAGTCCTCGATCCCGGGCGCGGTCGGA
CCAAGACCGGCCAGTTCTTCGCCTATGCCCGCGACGATCGTCCCTTCGGCGGCGATGATCCGCCGGCGGTCGCCTACATCTACGCGCCGGACCGAAAGGC
GGTGCGCCCCACCGAGCATTTACAGGACTTCAACGGCATCCTGCAGGTCGATGGCTATAGCGGCTACAAGGCGCTCGCCGAGAAGGGACAGGTGCGTCTA
GCCTTCTGTTGGGCGCATGTCCGTCGGCGGTTCTATGAACTCGCCGCCAATGGACCTGCGTCGATCGCGACGGAGGCGTTGAAGCGCATCGCCGCGCTCT
ACCAGATCGAGACCACCATCCGCGGCCGGTCTCCTGACGAGCGTCGGAAAGTTCGGCAGGAGAAAAGCCTGCCACTCCTCGACGCGCTGGAAGCCTTCCT
GCGCGAGAAGCTCGCCCTGATCAGCCAGAAGACGAAGCTCGCCGAGGCGATCCGATATGCGCTGTCACGATGGGAGGGACTTTGCCGGTTCGCCGGTGAC
GGCACGATCGAGATCGACAACAACACCGTCGAGCGCTCGATCCGCCCATTGGCGCTGACGCGCAAGAATGCGCTCTTCGCCGGTTCCGACGGCGGTGCCC
GGCACTGGGCCGTCGTCGCCTCGCTCATCGAGACCTGCAAGCTAAACGACGTCGATCCGTACGCCTATCTCAGCGCCGTCATCACCTGCATCGTCGACGG
CCATCCCAACAGCGCGATCGACGACCTCATGCCGTGGACATTCAAGCCGAAAGTCGATCTCACCGCGGTGGCCTGAGGATGACGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
444 bp	147 aa	87	530	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MSMSCADAGTKPVRRFEVFTGAGRRRDWSDEEKAAIVAESYSGLESVCAVARRHGLSHSQLFTWRRKLRETALVKSDATATEPAFVPAIVEPSIIEKKPR
AKRKRSRSSGKAIPAVELEIDGVAVKIAKGADAKTIAAVIDALKGSA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
327 bp	108 aa	557	883	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MVATKPVDFRKGAEGLAALVRETMAADPFGGTIYVFRAKRADRIKLIFWDGTGLVLVAKRLEDGAFKWPAITDGVIRLTAAQLSALLEGLDWRRVHEART
TRVPEAVS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1533 bp	510 aa	944	2476	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MATALEALPDDPGTLKAMLIAERVRSERLEQIIKELQRHRFGRRAETLPEDQLLLGLEDVEQGVAVEEAEAESADPERKTERAAKRRANRGSLPAHLPRI
ETVVDIEDKSCPCCRHWLHVIGEDVAERLDIVPAQFRVLVTRRPRYGCRACEGVVVQAPAPNRLIEGGIPTEATVAQVLVSKYADHLPLYRQAQIYARQG
VRLDRSTLADWVGRAAFLLRPIHERLLGHLKASTKLFADETTAPVLDPGRGRTKTGQFFAYARDDRPFGGDDPPAVAYIYAPDRKAVRPTEHLQDFNGIL
QVDGYSGYKALAEKGQVRLAFCWAHVRRRFYELAANGPASIATEALKRIAALYQIETTIRGRSPDERRKVRQEKSLPLLDALEAFLREKLALISQKTKLA
EAIRYALSRWEGLCRFAGDGTIEIDNNTVERSIRPLALTRKNALFAGSDGGARHWAVVASLIETCKLNDVDPYAYLSAVITCIVDGHPNSAIDDLMPWTF
KPKVDLTAVA

Blast result :

Comments

ISFpe1 orf1, orf2 and orf3 are respectively 55%, 80% and 73% aa similar to ISApr6 orf1, orf2 and orf3.

References

1] ISfinder annotation (2008).
2] Giovannoni,S.J., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct Submission GenBank.