ISGdi5
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Gluconacetobacter diazotrophicus | Gluconacetobacter diazotrophicus PAl 5 |
DNA section
IS Length : 1166 bp
Ends
IR Length : 24/30
IRL : CCAAAGGCCGTAGCCACTGACTCGTGTGTGAAGTGACGAACGGCGCCGGC
IRR : CCAACGGTTATAGGTACTGACCCGTGTGTGCCCATTTCCTGAGTCCGATG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTGAATAAAAGCTATTTA | TA | TTAGAAATTGATGTGGCG | 2 |
DNA sequence
CCAAAGGCCGTAGCCACTGACTCGTGTGTGAAGTGACGAACGGCGCCGGCTCTGATTCTCTTGATTTTGGGAGGATCTGGCAGATGACGAGAGCGGTGAA
GCTACGGGATGACTATTCGGCCTCTGAGCTGAGGCGACTTGCGGCGCGCAGCCGGCAGGCGAACGCTGCGCGCCGGCTTCTGGCCCTGGCGGCGATCCGT
GACGGGGCCAGCCGCACCGATGCGGCCCGCGTAGGCGGCATGGACCGCCAGACGCTGCGGGACTGGGTTCACCGCTTCAATGCTGAAGGGCCGGATGGCC
TGCGCGATCATCTGCACGCAGGGCCGGCCTGCCGTCTGAGTGTCGCACAGCAGACCGAATTGAAGGCCCTGGTCGAGGCAGGCCCGGATCGGCAGCGCGA
TGGCGTGGTGCGCTGGCGCCGGGTCGATCTGCAACGCGTGATCGAAGAGCGCTTCGGCGTCGTCTATCATGAGCGTCATGTGTCCACTCTACTGAAACGG
CTTGGTTTTTCCTATGTCAGCGCCCGGCCACGTCACCCGGGTCAGGACGCTGGCGTCATGGAGGCATTCAAAAAAACTTCCCCCGCATCCTGAATGCCCA
TACCGGCCATCTGCCCAAGGGCAAGCCGATCGAGATCTGGTTCCAGGACGAGGCCCGGATCGGCCAGAAGAACGGCATCGTCCGACAATGGGCCCGGCGT
GGCACGCGGCCACGCCAGCCGGCTGATCAGCGCTACGAGAATGCCTGGCTGTTCGGCGCGATCTGTCCGGCGCGTGGCAAAGCCGCCGGCCTGGCGCTCC
CGTTCATCGGCACCGCCAGCATGCAACTGCACATCGAGGAAATCTCGCGCTGCGTCGTCCGCGGTGCCCACGCCGTCGTGCTGCTCGATCGCGCAGGATG
GCATACCACCGACAAGCTGAAGCTGCCCCGCAATATCAGCCTGATCTTCCTGCCGTCCCGCGCGCCCGAACTGAACCCGGTCGAAAATGTCTGGCAGTTC
CTGCGCGCCAACTGGCTGTCCAACACCGTCTTCGACGGCATCGATCATATCATCGACGCCGCCTGTTCCGCCTGGAACAAGCTTGCCGCCCTGCCTGACA
CCATCCGATCCATCGGACTCAGGAAATGGGCACACACGGGTCAGTACCTATAACCGTTGG
GCTACGGGATGACTATTCGGCCTCTGAGCTGAGGCGACTTGCGGCGCGCAGCCGGCAGGCGAACGCTGCGCGCCGGCTTCTGGCCCTGGCGGCGATCCGT
GACGGGGCCAGCCGCACCGATGCGGCCCGCGTAGGCGGCATGGACCGCCAGACGCTGCGGGACTGGGTTCACCGCTTCAATGCTGAAGGGCCGGATGGCC
TGCGCGATCATCTGCACGCAGGGCCGGCCTGCCGTCTGAGTGTCGCACAGCAGACCGAATTGAAGGCCCTGGTCGAGGCAGGCCCGGATCGGCAGCGCGA
TGGCGTGGTGCGCTGGCGCCGGGTCGATCTGCAACGCGTGATCGAAGAGCGCTTCGGCGTCGTCTATCATGAGCGTCATGTGTCCACTCTACTGAAACGG
CTTGGTTTTTCCTATGTCAGCGCCCGGCCACGTCACCCGGGTCAGGACGCTGGCGTCATGGAGGCATTCAAAAAAACTTCCCCCGCATCCTGAATGCCCA
TACCGGCCATCTGCCCAAGGGCAAGCCGATCGAGATCTGGTTCCAGGACGAGGCCCGGATCGGCCAGAAGAACGGCATCGTCCGACAATGGGCCCGGCGT
GGCACGCGGCCACGCCAGCCGGCTGATCAGCGCTACGAGAATGCCTGGCTGTTCGGCGCGATCTGTCCGGCGCGTGGCAAAGCCGCCGGCCTGGCGCTCC
CGTTCATCGGCACCGCCAGCATGCAACTGCACATCGAGGAAATCTCGCGCTGCGTCGTCCGCGGTGCCCACGCCGTCGTGCTGCTCGATCGCGCAGGATG
GCATACCACCGACAAGCTGAAGCTGCCCCGCAATATCAGCCTGATCTTCCTGCCGTCCCGCGCGCCCGAACTGAACCCGGTCGAAAATGTCTGGCAGTTC
CTGCGCGCCAACTGGCTGTCCAACACCGTCTTCGACGGCATCGATCATATCATCGACGCCGCCTGTTCCGCCTGGAACAAGCTTGCCGCCCTGCCTGACA
CCATCCGATCCATCGGACTCAGGAAATGGGCACACACGGGTCAGTACCTATAACCGTTGG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 510 bp | 169 aa | 84 | 593 | + | No |
Description : First part of the transposase
ORF sequence :
MTRAVKLRDDYSASELRRLAARSRQANAARRLLALAAIRDGASRTDAARVGGMDRQTLRDWVHRFNAEGPDGLRDHLHAGPACRLSVAQQTELKALVEAG
PDRQRDGVVRWRRVDLQRVIEERFGVVYHERHVSTLLKRLGFSYVSARPRHPGQDAGVMEAFKKTSPAS
PDRQRDGVVRWRRVDLQRVIEERFGVVYHERHVSTLLKRLGFSYVSARPRHPGQDAGVMEAFKKTSPAS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 681 bp | 226 aa | 473 | 1153 | + | No |
Description : Second part of the transposase
ORF sequence :
ASCVHSTETAWFFLCQRPATSPGSGRWRHGGIQKNFPRILNAHTGHLPKGKPIEIWFQDEARIGQKNGIVRQWARRGTRPRQPADQRYENAWLFGAICPA
RGKAAGLALPFIGTASMQLHIEEISRCVVRGAHAVVLLDRAGWHTTDKLKLPRNISLIFLPSRAPELNPVENVWQFLRANWLSNTVFDGIDHIIDAACSA
WNKLAALPDTIRSIGLRKWAHTGQYL
RGKAAGLALPFIGTASMQLHIEEISRCVVRGAHAVVLLDRAGWHTTDKLKLPRNISLIFLPSRAPELNPVENVWQFLRANWLSNTVFDGIDHIIDAACSA
WNKLAALPDTIRSIGLRKWAHTGQYL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1070 bp | 356 aa | 84 | 1153 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRAVKLRDDYSASELRRLAARSRQANAARRLLALAAIRDGASRTDAARVGGMDRQTLRDWVHRFNAEGPDGLRDHLHAGPACRLSVAQQTELKALVEAG
PDRQRDGVVRWRRVDLQRVIEERFGVVYHERHVSTLLKRLGFSYVSARPRHPGQDAGVMEAFKKNFPRILNAHTGHLPKGKPIEIWFQDEARIGQKNGIV
RQWARRGTRPRQPADQRYENAWLFGAICPARGKAAGLALPFIGTASMQLHIEEISRCVVRGAHAVVLLDRAGWHTTDKLKLPRNISLIFLPSRAPELNPV
ENVWQFLRANWLSNTVFDGIDHIIDAACSAWNKLAALPDTIRSIGLRKWAHTGQYL
PDRQRDGVVRWRRVDLQRVIEERFGVVYHERHVSTLLKRLGFSYVSARPRHPGQDAGVMEAFKKNFPRILNAHTGHLPKGKPIEIWFQDEARIGQKNGIV
RQWARRGTRPRQPADQRYENAWLFGAICPARGKAAGLALPFIGTASMQLHIEEISRCVVRGAHAVVLLDRAGWHTTDKLKLPRNISLIFLPSRAPELNPV
ENVWQFLRANWLSNTVFDGIDHIIDAACSAWNKLAALPDTIRSIGLRKWAHTGQYL
Blast result :
Comments
ISGdi5 is 68% aa similar to ISAli3 . The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Miriam Land (2008) Direct submission.