ISGdi6

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Gluconacetobacter diazotrophicus
Gluconacetobacter diazotrophicus PAl 5
DNA section
IS Length : 1051 bp

Ends


IR Length : 14

IRL : GAGCGTGATGATTTCGGTCTGAATCGGAATATGGGTACCCAGATTGCCGA
IRR : GAGCGTGATGATTTTAGGTTGGTCCATATCCTGAGTTTATGAAATAATTG

Insertion site


Left flankDirect repeatRight flankDR Length
GCCGAGGCTGACCAACGTTAGCAGGCGGTCGAGGCTT2
ACGATGGAGACATTCTTTTAATAAGAACGGATGTGCA2
TTTGAAAGCC TGGAACCCTAATCCCCATGGACATGTT2
GCTGAGTTGC CGTGCGATTAGAGACACGATTTTTCTT2
CCCAATCCGA CAGCCTGCTAGTTCTTGCGGCGGGTGG2
ACGATGGAGA CATTCTTTTAATAAGAACGGATGTGCAT2

DNA sequence

GAGCGTGATGATTTCGGTCTGAATCGGAATATGGGTACCCAGATTGCCGAAAATCTGATTCAACATGCTGTCTGGATTGGAGGCCAGCATGGATGGCGCG
TCCTTATTCGATGGATCTTCGCGACCGGGTTGTGGCAGCCGTGGAGACAGACGGCCTCTCCTGCCACGAGGCCGCGAAGCGCTACGGTGTAGCACCCAGC
ACCGCGATCCGGTGGATGCAGAGCTTTCGGCGGACCGGCAGCGTTTCCCCTGGCCAGATGGGCGGCCACAAGCCGAAACGGCTGACGGGTGCGCATCGGG
AATGGCTGATCGAGCGTTGCAAGGCGGGGGGCTTTACCCTGCGGGGCTTGGTCGCAGAACTTGCCGGGCGCGGGCTCAGGGTCGATTATCGCTCTGTGTG
GGTCTTCGTCCACGACGAGGGGCTCAGCTTCAAAAAAAACACTCGTCGCAGCCGAACAGGACCGTCCTGATGTCGCCCGACACCGGGCGCGATGGCAAAA
ATACCAACACCTCATAGATCCCGCCCGCCTGGTGTTCATCGATGAAACCTGGACCAAAACCAATATGGCGCCGCTACGCGGCTGGGCGCCGCGTGGCGAA
CGGCTCCGGGCCAAAGTGCCTTGGGGTCATTGGAACACCATGACCTTTATTGCCGCCCTGCGGGTCGATCGGATCGATGCGCCGTGGCTGCTCGATGGTC
CGATCAACGGCGAAAGCTTCCGCATCTACGTCGAGAATGAACTCGTGCCGACGCTCAGGCCTGGTGACATCGTGATCGCTGACAACCTCGGCAGCCACAA
AAACAAGGCAGCACGCGCAGCCATCCGACAGGTCGGCGCGCGCCTGATCCTCCTGCCAAAATACTCCCCAGACCTGAATCCCATTGAACAGGTCTTTGCC
AAGCTCAAGCACCTGCTCCGCAAGGCCGCGGCTCGAAGCCGCGACGCAGTCAGTTCAACCATCGGCGAACTCCTGCGCAGCTACGCTCCCAGCGAATGCG
CCAATTATTTCATAAACTCAGGATATGGACCAACCTAAAATCATCACGCTC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
378 bp125 aa93470+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MARPYSMDLRDRVVAAVETDGLSCHEAAKRYGVAPSTAIRWMQSFRRTGSVSPGQMGGHKPKRLTGAHREWLIERCKAGGFTLRGLVAELAGRGLRVDYR
SVWVFVHDEGLSFKKNTRRSRTGPS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
729 bp242 aa3101038+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SSVARRGALPCGAWSQNLPGAGSGSIIALCGSSSTTRGSASKKTLVAAEQDRPDVARHRARWQKYQHLIDPARLVFIDETWTKTNMAPLRGWAPRGERLR
AKVPWGHWNTMTFIAALRVDRIDAPWLLDGPINGESFRIYVENELVPTLRPGDIVIADNLGSHKNKAARAAIRQVGARLILLPKYSPDLNPIEQVFAKLK
HLLRKAAARSRDAVSSTIGELLRSYAPSECANYFINSGYGPT

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
946 bp314 aa931038+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARPYSMDLRDRVVAAVETDGLSCHEAAKRYGVAPSTAIRWMQSFRRTGSVSPGQMGGHKPKRLTGAHREWLIERCKAGGFTLRGLVAELAGRGLRVDYR
SVWVFVHDEGLSFKKTLVAAEQDRPDVARHRARWQKYQHLIDPARLVFIDETWTKTNMAPLRGWAPRGERLRAKVPWGHWNTMTFIAALRVDRIDAPWLL
DGPINGESFRIYVENELVPTLRPGDIVIADNLGSHKNKAARAAIRQVGARLILLPKYSPDLNPIEQVFAKLKHLLRKAAARSRDAVSSTIGELLRSYAPS
ECANYFINSGYGPT

 

Blast result :
Comments
ISGdi6 is 81% aa similar to ISRm2011-2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Miriam Land (2008) Direct submission.