ISSbe3
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_ABIC00000000 | ND | Shewanella benthica | Shewanella benthica KT99 |
DNA section
IS Length : 1714 bp
Ends
IR Length : 131/144
IRL : GTAACCGTTCACCAGCCCCTTATTGACACGATCAATTGATGATCAATAAG
IRR : GTAACCGTTCACCAGCCCCTTATTGACACGATCAATTGATGATCGATAAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTAAATAAAG | ATTCACAACA | 0 |
DNA sequence
GTAACCGTTCACCAGCCCCTTATTGACACGATCAATTGATGATCAATAAGGTTTGGTCATATCGCTATTGAACGATCTCTTTGCCGTGTCAAAATAGCTT
CATGAAAAAGAAAAAACAATTCGCAACAGAGCCGCCTACGGTTGCTGAACTCGGTGAAAACAAAGCAAACTTACTCATTCAAGAACTGTGGGAAAAGTTA
CGTGAATATGAAGACCGCCTAGCATTGAGCTCTAGAAACTCATCCAAATCACCATCCTCTGACTCCCCTGCGGATAAGGCTGAGCGAAAAAAGCTCAAAC
GCCTCGTAGTGGAAATAAGGTTGGCGCTCAACCTGGACATACGGGGCATAAAAGACCATTAGCGGAACTCAGCGCAACCGATGAGCAAGTCGCTTGCTTA
CCCGATTCATGTTGTTCTGATTGTGGTGGCGAGGTCATTGCCAACCCGACTCCAAGCTATCGTCATCAGGTATTTGAATTACCTAAACCTGCACTTGATA
TAACTGAATACCAGTTATTTCACGGGCGTTGTCAGCAGTGCAACACAGTGAGTAAAGGAGCCTTGCCTGAAGAGGCTCCCGATGGTCAGATGGGGCCACG
ATTGCTCAGTTACGTGGCTGTGCTCAGTGGCTTGTATCACCTGAGCGTACGTAAAATTCAACGTTTACTGCAAGACCAATACGGGACTCATTTCTCTACC
GGTCTGATTTCTGAAGCGCAAGGGCGAGTCAGTAGTATGCTGACGCCGACACACCAAGCTCTGCATCAACACATAAAAAAAGCGTCATTAGTGCATGTTG
ACGAGACGACGCACAATCGTAATGGTGAAGAGCATACCCGCTGGGTCTGGTTGTTATCGAGTGAGGATGCCGTATTCCAACAGGTGAAATATTTTCGTAA
TAAGGATGCAGCCAAATCGATATTAGGCGAGCATACCCAAGCGATTGTCGTGAGTGACCAATGTCCCAGCTACAACTGGATTGAGCCCGAACGGCATCAA
TTTTGCCTTGACCATGTGCAGCGTAATTTACAACAAATGGCCGATTATTCAGGTAAGGGGCTTACCGCGAGTATCGGCAATCGACTGGTATTGCTATTCA
AGTCCGTATTTCGCACTCAACATAGATATGAAGCCGGTGACGTTGACGAAATAATGTGGCGTCGGCGAATGCAACGACTAAGGCGCAATATTAAGCGCTG
GCTTGAGTGCGGGGAAAATGTGCCCGCTTCACGCTACTCAGGCCGATGCCGGCATATTCTCAAATATGAGCAAGGTTTATGGGTTTTTCTCAATCACCCA
GGGACACCTTTGACCAATAACGAAGCCGAGCGTTGTTTGCGTGGCAGCGTGATCATGCGCAAGATCTGCTATGGCACCAGTTCAGATAGGGGTGAGAAGT
TTCGATCTCGGATCCTCTCAGTCGTTGAAACGTGCAAGAAGCGCAAGTTATCCCCGATAACAGTGATCAGCACTATCATTGCGGGCGTGGTGGGTAAATG
CGACTACCCAGACGTATTTGGACTAACCTCAGCTTAGTCAATCCCCCTCTGGTGAATGCTTACATATTATCAATCGAAGGCGGCTCGTGCTCGAATTGTT
TTTTCTTTTTCATGAAGCCATTTTCACATGGAAAAGAGATCGTTCAATAGCTATATGACCAAACCTTATCGATCATCAATTGATCGTGTCAATAAGGGGC
TGGTGAACGGTTAC
CATGAAAAAGAAAAAACAATTCGCAACAGAGCCGCCTACGGTTGCTGAACTCGGTGAAAACAAAGCAAACTTACTCATTCAAGAACTGTGGGAAAAGTTA
CGTGAATATGAAGACCGCCTAGCATTGAGCTCTAGAAACTCATCCAAATCACCATCCTCTGACTCCCCTGCGGATAAGGCTGAGCGAAAAAAGCTCAAAC
GCCTCGTAGTGGAAATAAGGTTGGCGCTCAACCTGGACATACGGGGCATAAAAGACCATTAGCGGAACTCAGCGCAACCGATGAGCAAGTCGCTTGCTTA
CCCGATTCATGTTGTTCTGATTGTGGTGGCGAGGTCATTGCCAACCCGACTCCAAGCTATCGTCATCAGGTATTTGAATTACCTAAACCTGCACTTGATA
TAACTGAATACCAGTTATTTCACGGGCGTTGTCAGCAGTGCAACACAGTGAGTAAAGGAGCCTTGCCTGAAGAGGCTCCCGATGGTCAGATGGGGCCACG
ATTGCTCAGTTACGTGGCTGTGCTCAGTGGCTTGTATCACCTGAGCGTACGTAAAATTCAACGTTTACTGCAAGACCAATACGGGACTCATTTCTCTACC
GGTCTGATTTCTGAAGCGCAAGGGCGAGTCAGTAGTATGCTGACGCCGACACACCAAGCTCTGCATCAACACATAAAAAAAGCGTCATTAGTGCATGTTG
ACGAGACGACGCACAATCGTAATGGTGAAGAGCATACCCGCTGGGTCTGGTTGTTATCGAGTGAGGATGCCGTATTCCAACAGGTGAAATATTTTCGTAA
TAAGGATGCAGCCAAATCGATATTAGGCGAGCATACCCAAGCGATTGTCGTGAGTGACCAATGTCCCAGCTACAACTGGATTGAGCCCGAACGGCATCAA
TTTTGCCTTGACCATGTGCAGCGTAATTTACAACAAATGGCCGATTATTCAGGTAAGGGGCTTACCGCGAGTATCGGCAATCGACTGGTATTGCTATTCA
AGTCCGTATTTCGCACTCAACATAGATATGAAGCCGGTGACGTTGACGAAATAATGTGGCGTCGGCGAATGCAACGACTAAGGCGCAATATTAAGCGCTG
GCTTGAGTGCGGGGAAAATGTGCCCGCTTCACGCTACTCAGGCCGATGCCGGCATATTCTCAAATATGAGCAAGGTTTATGGGTTTTTCTCAATCACCCA
GGGACACCTTTGACCAATAACGAAGCCGAGCGTTGTTTGCGTGGCAGCGTGATCATGCGCAAGATCTGCTATGGCACCAGTTCAGATAGGGGTGAGAAGT
TTCGATCTCGGATCCTCTCAGTCGTTGAAACGTGCAAGAAGCGCAAGTTATCCCCGATAACAGTGATCAGCACTATCATTGCGGGCGTGGTGGGTAAATG
CGACTACCCAGACGTATTTGGACTAACCTCAGCTTAGTCAATCCCCCTCTGGTGAATGCTTACATATTATCAATCGAAGGCGGCTCGTGCTCGAATTGTT
TTTTCTTTTTCATGAAGCCATTTTCACATGGAAAAGAGATCGTTCAATAGCTATATGACCAAACCTTATCGATCATCAATTGATCGTGTCAATAAGGGGC
TGGTGAACGGTTAC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 261 bp | 86 aa | 102 | 362 | + | No |
Description : First part of the transposase
ORF sequence :
MKKKKQFATEPPTVAELGENKANLLIQELWEKLREYEDRLALSSRNSSKSPSSDSPADKAERKKLKRLVVEIRLALNLDIRGIKDH
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1254 bp | 417 aa | 284 | 1537 | + | No |
Description : Second part of the transposase
ORF sequence :
AKKAQTPRSGNKVGAQPGHTGHKRPLAELSATDEQVACLPDSCCSDCGGEVIANPTPSYRHQVFELPKPALDITEYQLFHGRCQQCNTVSKGALPEEAPD
GQMGPRLLSYVAVLSGLYHLSVRKIQRLLQDQYGTHFSTGLISEAQGRVSSMLTPTHQALHQHIKKASLVHVDETTHNRNGEEHTRWVWLLSSEDAVFQQ
VKYFRNKDAAKSILGEHTQAIVVSDQCPSYNWIEPERHQFCLDHVQRNLQQMADYSGKGLTASIGNRLVLLFKSVFRTQHRYEAGDVDEIMWRRRMQRLR
RNIKRWLECGENVPASRYSGRCRHILKYEQGLWVFLNHPGTPLTNNEAERCLRGSVIMRKICYGTSSDRGEKFRSRILSVVETCKKRKLSPITVISTIIA
GVVGKCDYPDVFGLTSA
GQMGPRLLSYVAVLSGLYHLSVRKIQRLLQDQYGTHFSTGLISEAQGRVSSMLTPTHQALHQHIKKASLVHVDETTHNRNGEEHTRWVWLLSSEDAVFQQ
VKYFRNKDAAKSILGEHTQAIVVSDQCPSYNWIEPERHQFCLDHVQRNLQQMADYSGKGLTASIGNRLVLLFKSVFRTQHRYEAGDVDEIMWRRRMQRLR
RNIKRWLECGENVPASRYSGRCRHILKYEQGLWVFLNHPGTPLTNNEAERCLRGSVIMRKICYGTSSDRGEKFRSRILSVVETCKKRKLSPITVISTIIA
GVVGKCDYPDVFGLTSA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1436 bp | 478 aa | 102 | 1537 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKKKQFATEPPTVAELGENKANLLIQELWEKLREYEDRLALSSRNSSKSPSSDSPADKAERKKAQTPRSGNKVGAQPGHTGHKRPLAELSATDEQVACL
PDSCCSDCGGEVIANPTPSYRHQVFELPKPALDITEYQLFHGRCQQCNTVSKGALPEEAPDGQMGPRLLSYVAVLSGLYHLSVRKIQRLLQDQYGTHFST
GLISEAQGRVSSMLTPTHQALHQHIKKASLVHVDETTHNRNGEEHTRWVWLLSSEDAVFQQVKYFRNKDAAKSILGEHTQAIVVSDQCPSYNWIEPERHQ
FCLDHVQRNLQQMADYSGKGLTASIGNRLVLLFKSVFRTQHRYEAGDVDEIMWRRRMQRLRRNIKRWLECGENVPASRYSGRCRHILKYEQGLWVFLNHP
GTPLTNNEAERCLRGSVIMRKICYGTSSDRGEKFRSRILSVVETCKKRKLSPITVISTIIAGVVGKCDYPDVFGLTSA
PDSCCSDCGGEVIANPTPSYRHQVFELPKPALDITEYQLFHGRCQQCNTVSKGALPEEAPDGQMGPRLLSYVAVLSGLYHLSVRKIQRLLQDQYGTHFST
GLISEAQGRVSSMLTPTHQALHQHIKKASLVHVDETTHNRNGEEHTRWVWLLSSEDAVFQQVKYFRNKDAAKSILGEHTQAIVVSDQCPSYNWIEPERHQ
FCLDHVQRNLQQMADYSGKGLTASIGNRLVLLFKSVFRTQHRYEAGDVDEIMWRRRMQRLRRNIKRWLECGENVPASRYSGRCRHILKYEQGLWVFLNHP
GTPLTNNEAERCLRGSVIMRKICYGTSSDRGEKFRSRILSVVETCKKRKLSPITVISTIIAGVVGKCDYPDVFGLTSA
Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISSbe3 (orfAB) is 59% aa similar to ISAma4.
ISSbe3 (orfAB) is 59% aa similar to ISAma4.
References
1] ISfinder annotation (2008).
2] Yayanos,A., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2007) Direct Submission GenBank.
2] Yayanos,A., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2007) Direct Submission GenBank.