ISSba13
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009052 | ND | Shewanella baltica | Shewanella baltica OS155 |
DNA section
IS Length : 1670 bp
Ends
IR Length : 19/29
IRL : CCCCCTGTGTCAGGATTGTTGTCACCCCTAAATAATCATTATTTTTAAAG
IRR : CGGTGTTTTTCAAGATAGTTGTCACTCTTTGTTTAAAAATCAAGCTGCAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATTTTCATG | GGCA | ATGCCGGTGT | 4 |
| GCTTCTACGG | CATG | TGAGGCCACG | 4 |
| TTCGTTCAAC | CCTA | CAAGCTCCAA | 4 |
| ATCGATCAGA | GCTA | CTGCGACGCT | 4 |
| TGCGTTGTCC | ACTT | TGAAACGCCA | 4 |
| TTGTGTTACG | CAGC | CACTGCGCAA | 4 |
| TGACTCCGCC | CTGA | CTGAATCCTG | 4 |
| CAGAGGCTTC | AACT | CAAGCGCCCA | 4 |
| TAACTACACA | GGTG | TCAGCGCGCT | 4 |
| TCACAGCACA | CGAG | TTTGAGCAGG | 4 |
| AGCCTTGAAA | CACT | GAAGCTTTAT | 4 |
| CCAGCTAATA | GTAG | TGTGGGGCAT | 4 |
| CTGATCCCCT | GTGCTGCTTT | 0 | |
| CTATGCCTAC | TATCACGGCC | 0 | |
| CTGAAACATG | CGTTTATCAC | 0 | |
| GTCTAAGGGT | AGTAACTCAA | 0 | |
| AGTGCAATTT | ATCCCCGAGA | 0 | |
| AACATGAATG | AATACTTCTC | 0 |
DNA sequence
CCCCCTGTGTCAGGATTGTTGTCACCCCTAAATAATCATTATTTTTAAAGACAGGGTGCGGCAAAAGAGAATGATATGGCTCGGTATTCACCAGAACGTA
AAGAGGCGATCCTTAAAAAATTATTACCTCCTCACAACTTAACGGTCGCAGAGGTAGCTCGGGAAGAAGGCATAGCGGTGCAAACCTTGTATCATTGGCG
AGATATAGCCAGAAAAGAAGGTCGCCCCGTGCCAGGAAAAACATTAACAGCCGATGATTGGTCTGCAGAAGCTAAGTTTGCGGTACTCATTGAAACTGCT
CCGCTATCAGAAGCAGAAATAAGCCAGTATTGCCGTGAAAAAGGTTTGTACCGCGAACAAGTACAACAGTGGAAGTTGGAGTGCTTGTCTGGTTTTCAAA
CCTCAGAAGTACAAACTAAAACCATTAAACAGCAGGCCAAGGCTGACAAAGCCGAAATCAAATCGCTGCAACGGGAATTACGCTACAAGGAGAAAGCGTT
GGCAGAAGCTGCCGCATTACTGGTGCTCAGAAAAAAGCTCAATGTGCTCTGGGGGGACGACAGCGAGGAGAGCTAACCCCTTTGCCTGAGCGCATAGCAT
TGATTGCCTTAATTCAAGAGGCGTATACCCAGGGTGCTCGCCTTTATAAGGCTTGTGCTGAAACGGGCCTCAGTAAGCGTACCTATCGACGTTGGTATCG
AGAAGGTCAAGTGCAAGCTGATTTAAGGCCAACAGCAGCTCGTCCAGAGCCGGCCAATAAACTCGAAGAACATGAGCGACAACAGATATTAAGTGTGTGT
AATCAAGCGGAGTACGCCAGCTTACCCCCGTCGCAAATTGTGCCAACATTATTAGATAACGGGATTTATATCGCCTCGGAATCCAGCTTCTACAGAGTGT
TAAATGCTCATGGTCAACTTAACCATCGTGGCCGCACTCAGGCTACGGTCAATCGAAGCAAACCGTTGAGTTATCGGGCAGATGGGCCGAATCAAGTGTG
GTCATGGGACATCACTTATTTGGCCTCAACCGTTAAAGGCCAGTTCTACTATCTGTACCTGTTTGAAGATATTTACAGTAGAAAAATAGTGGGTTACGAA
GTTCATGAGCAAGAAAATGGGGAATATGCAGCCGCACTAATCCAACGCAGTCTGTTACGCGAGCAATGCTTTAACACGCCATTAGTGCTGCATTCTGATA
ATGGGGCGCCGATGAAGTCACTGACCATGAAAGCCAAGCTGGAAGAGCTCGGTATTACGGCATCATTAAGTCGGCCACGAGTGAGTAACGATAATCCGTT
TTCAGAGTCGTTATTTAAAACATTAAAATACCGTCCACAGTGGCCGGCATCAGGTTTTAGCTGCTTAGCAACAGCAAGAGAATGGGTGGAAAAGTTTGTA
AGCTGGTACAACGATGAGCATAAACACAGCCAGCTAAATTTTGTCTCACCAGGGCAACGTCATGCACTGCAAGATGGCGCTATTTTAGCCAAGCGCAAGA
AAGTGCTTGAGGCAGCGAAGGAAAGAAAACCGATGCGTTGGTCTACTGAGATTAGAAATTGTGAAGCGGTTGGCGCTGTAACACTCAACCCAGATAAGGC
ACCAGTAGAAGGCGTAATAAATGCAGCTTGATTTTTAAACAAAGAGTGACAACTATCTTGAAAAACACCG
AAGAGGCGATCCTTAAAAAATTATTACCTCCTCACAACTTAACGGTCGCAGAGGTAGCTCGGGAAGAAGGCATAGCGGTGCAAACCTTGTATCATTGGCG
AGATATAGCCAGAAAAGAAGGTCGCCCCGTGCCAGGAAAAACATTAACAGCCGATGATTGGTCTGCAGAAGCTAAGTTTGCGGTACTCATTGAAACTGCT
CCGCTATCAGAAGCAGAAATAAGCCAGTATTGCCGTGAAAAAGGTTTGTACCGCGAACAAGTACAACAGTGGAAGTTGGAGTGCTTGTCTGGTTTTCAAA
CCTCAGAAGTACAAACTAAAACCATTAAACAGCAGGCCAAGGCTGACAAAGCCGAAATCAAATCGCTGCAACGGGAATTACGCTACAAGGAGAAAGCGTT
GGCAGAAGCTGCCGCATTACTGGTGCTCAGAAAAAAGCTCAATGTGCTCTGGGGGGACGACAGCGAGGAGAGCTAACCCCTTTGCCTGAGCGCATAGCAT
TGATTGCCTTAATTCAAGAGGCGTATACCCAGGGTGCTCGCCTTTATAAGGCTTGTGCTGAAACGGGCCTCAGTAAGCGTACCTATCGACGTTGGTATCG
AGAAGGTCAAGTGCAAGCTGATTTAAGGCCAACAGCAGCTCGTCCAGAGCCGGCCAATAAACTCGAAGAACATGAGCGACAACAGATATTAAGTGTGTGT
AATCAAGCGGAGTACGCCAGCTTACCCCCGTCGCAAATTGTGCCAACATTATTAGATAACGGGATTTATATCGCCTCGGAATCCAGCTTCTACAGAGTGT
TAAATGCTCATGGTCAACTTAACCATCGTGGCCGCACTCAGGCTACGGTCAATCGAAGCAAACCGTTGAGTTATCGGGCAGATGGGCCGAATCAAGTGTG
GTCATGGGACATCACTTATTTGGCCTCAACCGTTAAAGGCCAGTTCTACTATCTGTACCTGTTTGAAGATATTTACAGTAGAAAAATAGTGGGTTACGAA
GTTCATGAGCAAGAAAATGGGGAATATGCAGCCGCACTAATCCAACGCAGTCTGTTACGCGAGCAATGCTTTAACACGCCATTAGTGCTGCATTCTGATA
ATGGGGCGCCGATGAAGTCACTGACCATGAAAGCCAAGCTGGAAGAGCTCGGTATTACGGCATCATTAAGTCGGCCACGAGTGAGTAACGATAATCCGTT
TTCAGAGTCGTTATTTAAAACATTAAAATACCGTCCACAGTGGCCGGCATCAGGTTTTAGCTGCTTAGCAACAGCAAGAGAATGGGTGGAAAAGTTTGTA
AGCTGGTACAACGATGAGCATAAACACAGCCAGCTAAATTTTGTCTCACCAGGGCAACGTCATGCACTGCAAGATGGCGCTATTTTAGCCAAGCGCAAGA
AAGTGCTTGAGGCAGCGAAGGAAAGAAAACCGATGCGTTGGTCTACTGAGATTAGAAATTGTGAAGCGGTTGGCGCTGTAACACTCAACCCAGATAAGGC
ACCAGTAGAAGGCGTAATAAATGCAGCTTGATTTTTAAACAAAGAGTGACAACTATCTTGAAAAACACCG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 501 bp | 166 aa | 76 | 576 | + | No |
Description : First part of the transposase
ORF sequence :
MARYSPERKEAILKKLLPPHNLTVAEVAREEGIAVQTLYHWRDIARKEGRPVPGKTLTADDWSAEAKFAVLIETAPLSEAEISQYCREKGLYREQVQQWK
LECLSGFQTSEVQTKTIKQQAKADKAEIKSLQRELRYKEKALAEAAALLVLRKKLNVLWGDDSEES
LECLSGFQTSEVQTKTIKQQAKADKAEIKSLQRELRYKEKALAEAAALLVLRKKLNVLWGDDSEES
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1185 bp | 394 aa | 447 | 1631 | + | No |
Description : Second part of the transposase
ORF sequence :
QSRNQIAATGITLQGESVGRSCRITGAQKKAQCALGGRQRGELTPLPERIALIALIQEAYTQGARLYKACAETGLSKRTYRRWYREGQVQADLRPTAARP
EPANKLEEHERQQILSVCNQAEYASLPPSQIVPTLLDNGIYIASESSFYRVLNAHGQLNHRGRTQATVNRSKPLSYRADGPNQVWSWDITYLASTVKGQF
YYLYLFEDIYSRKIVGYEVHEQENGEYAAALIQRSLLREQCFNTPLVLHSDNGAPMKSLTMKAKLEELGITASLSRPRVSNDNPFSESLFKTLKYRPQWP
ASGFSCLATAREWVEKFVSWYNDEHKHSQLNFVSPGQRHALQDGAILAKRKKVLEAAKERKPMRWSTEIRNCEAVGAVTLNPDKAPVEGVINAA
EPANKLEEHERQQILSVCNQAEYASLPPSQIVPTLLDNGIYIASESSFYRVLNAHGQLNHRGRTQATVNRSKPLSYRADGPNQVWSWDITYLASTVKGQF
YYLYLFEDIYSRKIVGYEVHEQENGEYAAALIQRSLLREQCFNTPLVLHSDNGAPMKSLTMKAKLEELGITASLSRPRVSNDNPFSESLFKTLKYRPQWP
ASGFSCLATAREWVEKFVSWYNDEHKHSQLNFVSPGQRHALQDGAILAKRKKVLEAAKERKPMRWSTEIRNCEAVGAVTLNPDKAPVEGVINAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1556 bp | 518 aa | 76 | 1631 | + | Yes |
Chemistry : DDE
ORF sequence :
MARYSPERKEAILKKLLPPHNLTVAEVAREEGIAVQTLYHWRDIARKEGRPVPGKTLTADDWSAEAKFAVLIETAPLSEAEISQYCREKGLYREQVQQWK
LECLSGFQTSEVQTKTIKQQAKADKAEIKSLQRELRYKEKALAEAAALLVLRKKAQCALGGRQRGELTPLPERIALIALIQEAYTQGARLYKACAETGLS
KRTYRRWYREGQVQADLRPTAARPEPANKLEEHERQQILSVCNQAEYASLPPSQIVPTLLDNGIYIASESSFYRVLNAHGQLNHRGRTQATVNRSKPLSY
RADGPNQVWSWDITYLASTVKGQFYYLYLFEDIYSRKIVGYEVHEQENGEYAAALIQRSLLREQCFNTPLVLHSDNGAPMKSLTMKAKLEELGITASLSR
PRVSNDNPFSESLFKTLKYRPQWPASGFSCLATAREWVEKFVSWYNDEHKHSQLNFVSPGQRHALQDGAILAKRKKVLEAAKERKPMRWSTEIRNCEAVG
AVTLNPDKAPVEGVINAA
LECLSGFQTSEVQTKTIKQQAKADKAEIKSLQRELRYKEKALAEAAALLVLRKKAQCALGGRQRGELTPLPERIALIALIQEAYTQGARLYKACAETGLS
KRTYRRWYREGQVQADLRPTAARPEPANKLEEHERQQILSVCNQAEYASLPPSQIVPTLLDNGIYIASESSFYRVLNAHGQLNHRGRTQATVNRSKPLSY
RADGPNQVWSWDITYLASTVKGQFYYLYLFEDIYSRKIVGYEVHEQENGEYAAALIQRSLLREQCFNTPLVLHSDNGAPMKSLTMKAKLEELGITASLSR
PRVSNDNPFSESLFKTLKYRPQWPASGFSCLATAREWVEKFVSWYNDEHKHSQLNFVSPGQRHALQDGAILAKRKKVLEAAKERKPMRWSTEIRNCEAVG
AVTLNPDKAPVEGVINAA
Blast result :
Comments
There are 17 copies of ISSba13 encoded in the chromosome (2 are interrupted) and 1 on plasmid 2 of Shewanella baltica OS155. The 2 ORFs encoded by ISSba13 are predicted to undergo programmed frameshifting during translation to produce peptide 3. The ISSba13 transposase has 86% identity with that of ISSde10_aa3. ISSba13 elements lacking direct repeats truncate, rather than interrupt, genes.
References
1] Romine, M. F. (2008). Direct submission.