ISShwo1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010506 | ND | Shewanella woodyi | Shewanella woodyi MS-32 |
DNA section
IS Length : 1308 bp
Ends
IR Length : 16/21
IRL : TGTCTTGTCCTGAAATGTGTTTACACATTTAGGACTTTATTATGACAACT
IRR : TGTCCCATCCTGAAATAGGTTGACGGTTTTAATTAGACCAGTTGCTCTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTTATTGGA | TAG | AGTTAAGAGT | 3 |
| TTTTATTCAA | TC | TGATTCATAA | 2 |
| TTTTGTATCT | GTG | TTATACCAAT | 3 |
| TTTTGTGTCT | GG | CCATAAATGT | 2 |
| TTTTATTGGA | TAG | AGTTAAGAGT | 3 |
DNA sequence
TGTCTTGTCCTGAAATGTGTTTACACATTTAGGACTTTATTATGACAACTCAAATCCCCACCCACGTTAAGCGAACACAACGAGATTATTCGTTAGGCTT
TAAATTACAAGTTGTAGCAGCCGTTGAAAAAGGCGACATGACTTATAAGCAAGCTCAAAGCATTTATGGTATCCAAGGTCGTTCAACCGTACTTACTTGG
CTCAGAAAGCACGGTAAGATGGATTGGACTCAACCAGTGAGAATAACCATGCCTAAGACCACCAAAGCTAAAGAAACGCCAGCTCAAAAAATAAAGCGCC
TTGAAAAAGAGCTTGAGGATGAACATTTACGTAATCTATTACTCAATGAAGCCGTTGATATCATTGATGCTGAGTATGGCGCAGGCCTAAGAAAAAAGTA
CTTAGCCAGGGAGCGAGAAGTCTTCAAAAACAGAAAGTAACAAGCTTAAATCGTGCTTGCGAGCTTCTTGGCATAACAAGACAAGCTATCTACCAAAGAG
AAACGAGAGAACTCTGCCGAGCTATAGAACTGGCCCCAGTAAAAGCAATGGTGCTTGATATCCGTCGCTTTATGCCACGTGTGGGTGGCAGGAAACTCTA
CTTTTTACTGAAACCCAAGTTCATCGAGAAAGGAATTAAACTTGGGCGAGATAACTTCTTTAGTTACTTGAAACGTGAAGGGTTATTGGTAAAACCTAAG
CATAACTATACCAAGACAACCCACAGTAAACACTGGATGAAGAAACACCCGAATTTACTCAAAGAGTTTACGCCTTTATCGCCTGAAGAGGTCTTTGTGA
GTGACATAACTTATGTTCAATCAGAGCAAGGTGTTCACTACTTGTCACTGGTGACGGATGCGTTTAGTCGTAAGATTATGGGTTATGAACTTAGTGATGA
GATGAAAGCAACTGATGTAGTTAAAGCCTTAAAAATGACAGTGAATAATCGCCAATATCAAGGCCATGCAGTTCATCATTCAGACAGAGGGTTACAGTAT
TGTTCAGCAGTCTATCAGTCAGCATTACAGAAAAATGGTATCCGAGCATCAATGACGGATGGGTATGATTGCTATCAAAACGCATTAGCGGAGCGAGTAA
ATGGTATTTTGAAGCAAGAGTTTTTGTTATATCCGTGCAGCAATCTTGATGAGTTAAAGCGACTCGTTGAAGAGTCAGTTTTTATATACAATGAAATGAG
GCCGCACTTGAGTTTAGGTATGAGAACACCAAATCAAGTGCATAAAAAAGACCAGCAGCAAGAGCAACTGGTCTAATTAAAACCGTCAACCTATTTCAGG
ATGGGACA
TAAATTACAAGTTGTAGCAGCCGTTGAAAAAGGCGACATGACTTATAAGCAAGCTCAAAGCATTTATGGTATCCAAGGTCGTTCAACCGTACTTACTTGG
CTCAGAAAGCACGGTAAGATGGATTGGACTCAACCAGTGAGAATAACCATGCCTAAGACCACCAAAGCTAAAGAAACGCCAGCTCAAAAAATAAAGCGCC
TTGAAAAAGAGCTTGAGGATGAACATTTACGTAATCTATTACTCAATGAAGCCGTTGATATCATTGATGCTGAGTATGGCGCAGGCCTAAGAAAAAAGTA
CTTAGCCAGGGAGCGAGAAGTCTTCAAAAACAGAAAGTAACAAGCTTAAATCGTGCTTGCGAGCTTCTTGGCATAACAAGACAAGCTATCTACCAAAGAG
AAACGAGAGAACTCTGCCGAGCTATAGAACTGGCCCCAGTAAAAGCAATGGTGCTTGATATCCGTCGCTTTATGCCACGTGTGGGTGGCAGGAAACTCTA
CTTTTTACTGAAACCCAAGTTCATCGAGAAAGGAATTAAACTTGGGCGAGATAACTTCTTTAGTTACTTGAAACGTGAAGGGTTATTGGTAAAACCTAAG
CATAACTATACCAAGACAACCCACAGTAAACACTGGATGAAGAAACACCCGAATTTACTCAAAGAGTTTACGCCTTTATCGCCTGAAGAGGTCTTTGTGA
GTGACATAACTTATGTTCAATCAGAGCAAGGTGTTCACTACTTGTCACTGGTGACGGATGCGTTTAGTCGTAAGATTATGGGTTATGAACTTAGTGATGA
GATGAAAGCAACTGATGTAGTTAAAGCCTTAAAAATGACAGTGAATAATCGCCAATATCAAGGCCATGCAGTTCATCATTCAGACAGAGGGTTACAGTAT
TGTTCAGCAGTCTATCAGTCAGCATTACAGAAAAATGGTATCCGAGCATCAATGACGGATGGGTATGATTGCTATCAAAACGCATTAGCGGAGCGAGTAA
ATGGTATTTTGAAGCAAGAGTTTTTGTTATATCCGTGCAGCAATCTTGATGAGTTAAAGCGACTCGTTGAAGAGTCAGTTTTTATATACAATGAAATGAG
GCCGCACTTGAGTTTAGGTATGAGAACACCAAATCAAGTGCATAAAAAAGACCAGCAGCAAGAGCAACTGGTCTAATTAAAACCGTCAACCTATTTCAGG
ATGGGACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 399 bp | 132 aa | 42 | 440 | + | No |
Description : First part of the transposase
ORF sequence :
MTTQIPTHVKRTQRDYSLGFKLQVVAAVEKGDMTYKQAQSIYGIQGRSTVLTWLRKHGKMDWTQPVRITMPKTTKAKETPAQKIKRLEKELEDEHLRNLL
LNEAVDIIDAEYGAGLRKKYLAREREVFKNRK
LNEAVDIIDAEYGAGLRKKYLAREREVFKNRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 903 bp | 300 aa | 374 | 1276 | + | No |
Description : Second part of the transposase
ORF sequence :
VWRRPKKKVLSQGARSLQKQKVTSLNRACELLGITRQAIYQRETRELCRAIELAPVKAMVLDIRRFMPRVGGRKLYFLLKPKFIEKGIKLGRDNFFSYLK
REGLLVKPKHNYTKTTHSKHWMKKHPNLLKEFTPLSPEEVFVSDITYVQSEQGVHYLSLVTDAFSRKIMGYELSDEMKATDVVKALKMTVNNRQYQGHAV
HHSDRGLQYCSAVYQSALQKNGIRASMTDGYDCYQNALAERVNGILKQEFLLYPCSNLDELKRLVEESVFIYNEMRPHLSLGMRTPNQVHKKDQQQEQLV
REGLLVKPKHNYTKTTHSKHWMKKHPNLLKEFTPLSPEEVFVSDITYVQSEQGVHYLSLVTDAFSRKIMGYELSDEMKATDVVKALKMTVNNRQYQGHAV
HHSDRGLQYCSAVYQSALQKNGIRASMTDGYDCYQNALAERVNGILKQEFLLYPCSNLDELKRLVEESVFIYNEMRPHLSLGMRTPNQVHKKDQQQEQLV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1235 bp | 411 aa | 42 | 1276 | + | Yes |
Chemistry : DDE
ORF sequence :
MTTQIPTHVKRTQRDYSLGFKLQVVAAVEKGDMTYKQAQSIYGIQGRSTVLTWLRKHGKMDWTQPVRITMPKTTKAKETPAQKIKRLEKELEDEHLRNLL
LNEAVDIIDAEYGAGLRKKVLSQGARSLQKQKVTSLNRACELLGITRQAIYQRETRELCRAIELAPVKAMVLDIRRFMPRVGGRKLYFLLKPKFIEKGIK
LGRDNFFSYLKREGLLVKPKHNYTKTTHSKHWMKKHPNLLKEFTPLSPEEVFVSDITYVQSEQGVHYLSLVTDAFSRKIMGYELSDEMKATDVVKALKMT
VNNRQYQGHAVHHSDRGLQYCSAVYQSALQKNGIRASMTDGYDCYQNALAERVNGILKQEFLLYPCSNLDELKRLVEESVFIYNEMRPHLSLGMRTPNQV
HKKDQQQEQLV
LNEAVDIIDAEYGAGLRKKVLSQGARSLQKQKVTSLNRACELLGITRQAIYQRETRELCRAIELAPVKAMVLDIRRFMPRVGGRKLYFLLKPKFIEKGIK
LGRDNFFSYLKREGLLVKPKHNYTKTTHSKHWMKKHPNLLKEFTPLSPEEVFVSDITYVQSEQGVHYLSLVTDAFSRKIMGYELSDEMKATDVVKALKMT
VNNRQYQGHAVHHSDRGLQYCSAVYQSALQKNGIRASMTDGYDCYQNALAERVNGILKQEFLLYPCSNLDELKRLVEESVFIYNEMRPHLSLGMRTPNQV
HKKDQQQEQLV
Blast result :
Comments
There are 5 full length copies of ISShwo1 encoded in the Shewanella woodyi genome. The two open reading frames in this element are predicted to undergo programmed frameshifting to produce peptide 3. The ISShwo1 transposase has 63% identity to ISSba4_aa3.
References
1] Romine, M. F. (2008) Direct submission.