ISSba15
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009052 | ND | Shewanella baltica | Shewanella baltica OS155 |
DNA section
IS Length : 1643 bp
Ends
IR Length : 23/32
IRL : CCCCCTGTGTCAGGATAGTTGTCGCCTCAATTCATTTACAAATAAACAGG
IRR : CGGCGTTTTTCAAGACAGTTGTCGCTTAAATTGTTAAGCAGCTTCTTTAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTCTGAGTTT | GGAAGGCTAA | 0 | |
| CAGAAAGATG | CATC | AACAGTCGAG | 4 |
| TGAAAATGTG | GTTC | ATTTAATTTC | 4 |
| TGTCTTTGAG | GATA | TAGGAGGACA | 4 |
| TATTTTTGTA | TAAC | TACCAGAACT | 4 |
| AATCGTTCAG | TAAA | TAATGTTTCG | 4 |
| AGCTTGCTGG | ATTT | CATCGACAAA | 4 |
DNA sequence
CCCCCTGTGTCAGGATAGTTGTCGCCTCAATTCATTTACAAATAAACAGGGAGCGGTAAGTTACAGCAAGTGAATTATCAAAGGCATTCAGGTCAATTTA
AAGAAGCTATATTAAACAAGCTTAGTCAAAGTGGTTTGTCGGTTCGTCAGTTTGCAGAGCAAGAAGGCATTAATCTCTCAACCCTGTATAGTTGGCAAAA
GCAATTTAATACCTCAGGGTTAAACGTGTCAAAAGTCAGTTCATCGGATAAGTGGTCAAGTGAAGAAAAGTTTTCAGTGGTATTGGAAACAGCCTCTCTG
TCAGCAGTTGAGTTAAGTGAATATTGCCGAGCTAAGGGGTTATATCCTGAGCAAGTCAACGGATGGAAGCAAGCTTGCATTGCCGGTAATACAAGCACAA
CACTGACAAGTAAAGCCAAGACAACACCTGGACAGAAGACTGACAAAAAACGCATTAACGAGCTAGAACGTGAGTTACGTAGAAAGGATAAAGCGTTAGC
GGAAGCGGCTGCTTTACTCGTACTCGGAAAAAAGTTCGATGCCTATTGGAAGGAAAAAGAGGAAGACTGATTTCGCTGACCGATAGGCAGAAGCATGCGC
AGTGGATCTGCACAGCAGTAGCATCAGGCGCAAGACAAGATAAGGCCTGTGACGTGATTGGATTATCAAGTAGAACCTTGCAGCGTTGGCGAATAGACGG
TGACATTAGCGCAGATAAAAGGCCAACGGCAACGAGGCCGGAGCCTGGAAATAAACTGAGTCAAGCAGAAAGACAAGCCGTGATTGATGTGTGTAATAGT
GAAGAGTTTGCCTCATTACCACCAAGCCAAATTGTCCCGATACTCGCCGATAGAGGTGAGTATATTGCTTCGGAGTCGAGTTTTTACCGTGTATTGCATA
CTGAAAACATGCTGCACCACCGAGGCAAAGCTAAGCCGAGAAATGTTCATAAAAAGCCAACCAGTTATACGGCTAAAAAAGCGAACGAAGTGTGGAGTTG
GGATATCAGTTACCTGCCTACAACGGTGATAGGAAAACACTATTATCTGTACATGATTGAAGATATTTACAGTCGAAAAATCGTTGGTTGGGAAGTTCAC
GACAGTGAAACCGGCGAGCAAGCAGCAGCACTACTAGAGCGCACCGTCTGGTCAGAGAAATGCTTAAAGAAAGACGTTGTGTTGCACTCAGATAATGGCG
CGCCCATGAAGAGCCTGACGATGCGAGCTAAGATGTACGATTTAGGCGTTGTGACCTCACGAAGCCGTCCAAGGGTAAGTAATGATAATCCGTACTCAGA
ATCACTATTTAGAACGGTAAAGTATCATCCACGTTGGCCTAGTGAAGGTTTTAAGTCATTAGAAGCAGCACGTAAATGGGTCAATGAATTTGTTTATTGG
TACAACAACGAGCATCGCCATAGCAGGATTAAGTTTGTGACACCAAACCAAAGGCATGATGGTCTTGATGTTGGAATACTGGCGAAGCGAAAAGCGTTGT
ATCAGACAAAAAGAAATGAACATCCAAAGCGATGGTCAAAAGAAGAGCGAAACTGGCAGCCAATAGGCGCTGTGGAGTTAAATCCAGAGCAACATAAAGA
AGCTGCTTAACAATTTAAGCGACAACTGTCTTGAAAAACGCCG
AAGAAGCTATATTAAACAAGCTTAGTCAAAGTGGTTTGTCGGTTCGTCAGTTTGCAGAGCAAGAAGGCATTAATCTCTCAACCCTGTATAGTTGGCAAAA
GCAATTTAATACCTCAGGGTTAAACGTGTCAAAAGTCAGTTCATCGGATAAGTGGTCAAGTGAAGAAAAGTTTTCAGTGGTATTGGAAACAGCCTCTCTG
TCAGCAGTTGAGTTAAGTGAATATTGCCGAGCTAAGGGGTTATATCCTGAGCAAGTCAACGGATGGAAGCAAGCTTGCATTGCCGGTAATACAAGCACAA
CACTGACAAGTAAAGCCAAGACAACACCTGGACAGAAGACTGACAAAAAACGCATTAACGAGCTAGAACGTGAGTTACGTAGAAAGGATAAAGCGTTAGC
GGAAGCGGCTGCTTTACTCGTACTCGGAAAAAAGTTCGATGCCTATTGGAAGGAAAAAGAGGAAGACTGATTTCGCTGACCGATAGGCAGAAGCATGCGC
AGTGGATCTGCACAGCAGTAGCATCAGGCGCAAGACAAGATAAGGCCTGTGACGTGATTGGATTATCAAGTAGAACCTTGCAGCGTTGGCGAATAGACGG
TGACATTAGCGCAGATAAAAGGCCAACGGCAACGAGGCCGGAGCCTGGAAATAAACTGAGTCAAGCAGAAAGACAAGCCGTGATTGATGTGTGTAATAGT
GAAGAGTTTGCCTCATTACCACCAAGCCAAATTGTCCCGATACTCGCCGATAGAGGTGAGTATATTGCTTCGGAGTCGAGTTTTTACCGTGTATTGCATA
CTGAAAACATGCTGCACCACCGAGGCAAAGCTAAGCCGAGAAATGTTCATAAAAAGCCAACCAGTTATACGGCTAAAAAAGCGAACGAAGTGTGGAGTTG
GGATATCAGTTACCTGCCTACAACGGTGATAGGAAAACACTATTATCTGTACATGATTGAAGATATTTACAGTCGAAAAATCGTTGGTTGGGAAGTTCAC
GACAGTGAAACCGGCGAGCAAGCAGCAGCACTACTAGAGCGCACCGTCTGGTCAGAGAAATGCTTAAAGAAAGACGTTGTGTTGCACTCAGATAATGGCG
CGCCCATGAAGAGCCTGACGATGCGAGCTAAGATGTACGATTTAGGCGTTGTGACCTCACGAAGCCGTCCAAGGGTAAGTAATGATAATCCGTACTCAGA
ATCACTATTTAGAACGGTAAAGTATCATCCACGTTGGCCTAGTGAAGGTTTTAAGTCATTAGAAGCAGCACGTAAATGGGTCAATGAATTTGTTTATTGG
TACAACAACGAGCATCGCCATAGCAGGATTAAGTTTGTGACACCAAACCAAAGGCATGATGGTCTTGATGTTGGAATACTGGCGAAGCGAAAAGCGTTGT
ATCAGACAAAAAGAAATGAACATCCAAAGCGATGGTCAAAAGAAGAGCGAAACTGGCAGCCAATAGGCGCTGTGGAGTTAAATCCAGAGCAACATAAAGA
AGCTGCTTAACAATTTAAGCGACAACTGTCTTGAAAAACGCCG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 501 bp | 166 aa | 70 | 570 | + | No |
Description : First part of the transposase
ORF sequence :
MNYQRHSGQFKEAILNKLSQSGLSVRQFAEQEGINLSTLYSWQKQFNTSGLNVSKVSSSDKWSSEEKFSVVLETASLSAVELSEYCRAKGLYPEQVNGWK
QACIAGNTSTTLTSKAKTTPGQKTDKKRINELERELRRKDKALAEAAALLVLGKKFDAYWKEKEED
QACIAGNTSTTLTSKAKTTPGQKTDKKRINELERELRRKDKALAEAAALLVLGKKFDAYWKEKEED
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 3883 bp | 372 aa | 5492 | 1610 | + | No |
Description : Second part of the transposase
ORF sequence :
SVSGSGCFTRTRKKVRCLLEGKRGRLISLTDRQKHAQWICTAVASGARQDKACDVIGLSSRTLQRWRIDGDISADKRPTATRPEPGNKLSQAERQAVIDV
CNSEEFASLPPSQIVPILADRGEYIASESSFYRVLHTENMLHHRGKAKPRNVHKKPTSYTAKKANEVWSWDISYLPTTVIGKHYYLYMIEDIYSRKIVGW
EVHDSETGEQAAALLERTVWSEKCLKKDVVLHSDNGAPMKSLTMRAKMYDLGVVTSRSRPRVSNDNPYSESLFRTVKYHPRWPSEGFKSLEAARKWVNEF
VYWYNNEHRHSRIKFVTPNQRHDGLDVGILAKRKALYQTKRNEHPKRWSKEERNWQPIGAVELNPEQHKEAA
CNSEEFASLPPSQIVPILADRGEYIASESSFYRVLHTENMLHHRGKAKPRNVHKKPTSYTAKKANEVWSWDISYLPTTVIGKHYYLYMIEDIYSRKIVGW
EVHDSETGEQAAALLERTVWSEKCLKKDVVLHSDNGAPMKSLTMRAKMYDLGVVTSRSRPRVSNDNPYSESLFRTVKYHPRWPSEGFKSLEAARKWVNEF
VYWYNNEHRHSRIKFVTPNQRHDGLDVGILAKRKALYQTKRNEHPKRWSKEERNWQPIGAVELNPEQHKEAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1541 bp | 513 aa | 70 | 1610 | + | Yes |
Chemistry : DDE
ORF sequence :
MNYQRHSGQFKEAILNKLSQSGLSVRQFAEQEGINLSTLYSWQKQFNTSGLNVSKVSSSDKWSSEEKFSVVLETASLSAVELSEYCRAKGLYPEQVNGWK
QACIAGNTSTTLTSKAKTTPGQKTDKKRINELERELRRKDKALAEAAALLVLGKKVRCLLEGKRGRLISLTDRQKHAQWICTAVASGARQDKACDVIGLS
SRTLQRWRIDGDISADKRPTATRPEPGNKLSQAERQAVIDVCNSEEFASLPPSQIVPILADRGEYIASESSFYRVLHTENMLHHRGKAKPRNVHKKPTSY
TAKKANEVWSWDISYLPTTVIGKHYYLYMIEDIYSRKIVGWEVHDSETGEQAAALLERTVWSEKCLKKDVVLHSDNGAPMKSLTMRAKMYDLGVVTSRSR
PRVSNDNPYSESLFRTVKYHPRWPSEGFKSLEAARKWVNEFVYWYNNEHRHSRIKFVTPNQRHDGLDVGILAKRKALYQTKRNEHPKRWSKEERNWQPIG
AVELNPEQHKEAA
QACIAGNTSTTLTSKAKTTPGQKTDKKRINELERELRRKDKALAEAAALLVLGKKVRCLLEGKRGRLISLTDRQKHAQWICTAVASGARQDKACDVIGLS
SRTLQRWRIDGDISADKRPTATRPEPGNKLSQAERQAVIDVCNSEEFASLPPSQIVPILADRGEYIASESSFYRVLHTENMLHHRGKAKPRNVHKKPTSY
TAKKANEVWSWDISYLPTTVIGKHYYLYMIEDIYSRKIVGWEVHDSETGEQAAALLERTVWSEKCLKKDVVLHSDNGAPMKSLTMRAKMYDLGVVTSRSR
PRVSNDNPYSESLFRTVKYHPRWPSEGFKSLEAARKWVNEFVYWYNNEHRHSRIKFVTPNQRHDGLDVGILAKRKALYQTKRNEHPKRWSKEERNWQPIG
AVELNPEQHKEAA
Blast result :
Comments
There are 7 copies of ISSba15 encoded in the Shewanella baltica OS155 chromosome. Peptide 3 is derived from the predicted programmed frameshift of Orf1 and Orf2. ISSba15 is 72% aa similar to ISPa31 (orf3).
References
1] Romine, M. F. (2008) Direct submission.