ISShes13
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008577 | ND | Shewanella sp. | Shewanella sp. ANA-3 |
DNA section
IS Length : 1305 bp
Ends
IR Length : 23/31
IRL : TGACCCGTCCTAAATAGCGTTGACACTTTTCAAACTCATTTTGGAGAGTG
IRR : TGTCTCGTCCTGAAATAAGTTGACACTTATTTCCACCTAAAACGCTCGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAATTTGATA | TAG | CAAAGCGTAG | 3 |
| TTTATCTGGG | AGT | AGGAAGGGCG | 3 |
| TTTTTATTGA | TGA | AACTTAAGAA | 3 |
| TATTTCATTG | ATC | TGTTCAGGTG | 3 |
DNA sequence
TGACCCGTCCTAAATAGCGTTGACACTTTTCAAACTCATTTTGGAGAGTGTAATGACTTCAACAATTAAACGAACTCAACGTGATTATTCCCTCGCTTTT
AAATTGGCGGTTGTCGACCAAGTGGAAAAAGGCGAGCTGACCTATAAGCAGGCACAAGACAAGTACGGTATTCAAGGCTGCTCCACCGTGTTAGTGTGGT
TACGCAAGCATGGCAAACTGGATTGGTCCCTAGGGACGCCGCCTACATCAATGCGAGGACAGTTGATGACTGAACCCACCACGCTCACCCCCGAGCAAAA
GATAAAAGCCCTCGAAGCCGAACTTGAAGATACTCGCATGAAAGCCGCGTTCTTCGAAGCCATTGTCGAAAAACTCAGAACTGACTACGGGATATCCGTC
GTAAAAAAGCCACCCGCGAAACGCTCCAAGAAAAAACGGCCATAGGTTATGGCGTCACCCAATGCTGTCGTTACCTTGGCATCAGTCGCCAGGCGTATTA
TCAACAATGCCAGCGGCAAATCGTCTCAGAGCTAAAGGAGCAAGAGGTGGTGCAAACGGTACAATATGAACGGTTATTTCAACCGCGAGTCGGTACGCGT
AAACTGCAGTATTTACTCAACAAAATGCATCAAATAGTGATAGGACGAGACCGGTTATTTCGCTGTCTTAGGGCGCATCGTTTGTTGGTCACGCCGAAAC
GGGCGTACCATAAAACGACCCATAGCCATCATCGATTTAGGTGTCATCCCAACTTGCTTAAGCCCAGTGAGCAGCAAGTGGACATCAGCCGCTCAGAACA
AGTCTGGGTGGCTGATATTACCTATCTGTCACTGCAAGATAATGAAGCCTATTTAAGCTTGGTGACCGATGCCTGGTCACGAAAAATTGTGGGTTATCAT
GTTGATGATAATCTTAAAACAGACGGGGTGCTAACGGCTTACAAACGGGCACTGAGACAGCGACAAGATAAAAGTAAACCGCTGATACACCACTCAGACC
GAGGGATACAGTACTGCTCAAACGCGTATCAAGCGGTTCACGCACAACATGGCGTGACATGCTCAATGACAGACGGATATGACTGCTATCAAAATGCCTT
AGCAGAGCGAGTGAATGGCATCTTGAAGCAGGAGTTTTTAACACATAAGCCCGCAACGCTGGAGGAGGCGCGACAAATGGTGGCAGAAGCGGTTGCCATC
TATAATCAACGACGGCCACATTTGGCCTTAAAATACAAAACGCCCGATGAGGTTCATCGAGCGTTTTAGGTGGAAATAAGTGTCAACTTATTTCAGGACG
AGACA
AAATTGGCGGTTGTCGACCAAGTGGAAAAAGGCGAGCTGACCTATAAGCAGGCACAAGACAAGTACGGTATTCAAGGCTGCTCCACCGTGTTAGTGTGGT
TACGCAAGCATGGCAAACTGGATTGGTCCCTAGGGACGCCGCCTACATCAATGCGAGGACAGTTGATGACTGAACCCACCACGCTCACCCCCGAGCAAAA
GATAAAAGCCCTCGAAGCCGAACTTGAAGATACTCGCATGAAAGCCGCGTTCTTCGAAGCCATTGTCGAAAAACTCAGAACTGACTACGGGATATCCGTC
GTAAAAAAGCCACCCGCGAAACGCTCCAAGAAAAAACGGCCATAGGTTATGGCGTCACCCAATGCTGTCGTTACCTTGGCATCAGTCGCCAGGCGTATTA
TCAACAATGCCAGCGGCAAATCGTCTCAGAGCTAAAGGAGCAAGAGGTGGTGCAAACGGTACAATATGAACGGTTATTTCAACCGCGAGTCGGTACGCGT
AAACTGCAGTATTTACTCAACAAAATGCATCAAATAGTGATAGGACGAGACCGGTTATTTCGCTGTCTTAGGGCGCATCGTTTGTTGGTCACGCCGAAAC
GGGCGTACCATAAAACGACCCATAGCCATCATCGATTTAGGTGTCATCCCAACTTGCTTAAGCCCAGTGAGCAGCAAGTGGACATCAGCCGCTCAGAACA
AGTCTGGGTGGCTGATATTACCTATCTGTCACTGCAAGATAATGAAGCCTATTTAAGCTTGGTGACCGATGCCTGGTCACGAAAAATTGTGGGTTATCAT
GTTGATGATAATCTTAAAACAGACGGGGTGCTAACGGCTTACAAACGGGCACTGAGACAGCGACAAGATAAAAGTAAACCGCTGATACACCACTCAGACC
GAGGGATACAGTACTGCTCAAACGCGTATCAAGCGGTTCACGCACAACATGGCGTGACATGCTCAATGACAGACGGATATGACTGCTATCAAAATGCCTT
AGCAGAGCGAGTGAATGGCATCTTGAAGCAGGAGTTTTTAACACATAAGCCCGCAACGCTGGAGGAGGCGCGACAAATGGTGGCAGAAGCGGTTGCCATC
TATAATCAACGACGGCCACATTTGGCCTTAAAATACAAAACGCCCGATGAGGTTCATCGAGCGTTTTAGGTGGAAATAAGTGTCAACTTATTTCAGGACG
AGACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 393 bp | 130 aa | 53 | 445 | + | No |
Description : First part of the transposase
ORF sequence :
MTSTIKRTQRDYSLAFKLAVVDQVEKGELTYKQAQDKYGIQGCSTVLVWLRKHGKLDWSLGTPPTSMRGQLMTEPTTLTPEQKIKALEAELEDTRMKAAF
FEAIVEKLRTDYGISVVKKPPAKRSKKKRP
FEAIVEKLRTDYGISVVKKPPAKRSKKKRP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 885 bp | 294 aa | 385 | 1269 | + | No |
Description : Second part of the transposase
ORF sequence :
LRDIRRKKATRETLQEKTAIGYGVTQCCRYLGISRQAYYQQCQRQIVSELKEQEVVQTVQYERLFQPRVGTRKLQYLLNKMHQIVIGRDRLFRCLRAHRL
LVTPKRAYHKTTHSHHRFRCHPNLLKPSEQQVDISRSEQVWVADITYLSLQDNEAYLSLVTDAWSRKIVGYHVDDNLKTDGVLTAYKRALRQRQDKSKPL
IHHSDRGIQYCSNAYQAVHAQHGVTCSMTDGYDCYQNALAERVNGILKQEFLTHKPATLEEARQMVAEAVAIYNQRRPHLALKYKTPDEVHRAF
LVTPKRAYHKTTHSHHRFRCHPNLLKPSEQQVDISRSEQVWVADITYLSLQDNEAYLSLVTDAWSRKIVGYHVDDNLKTDGVLTAYKRALRQRQDKSKPL
IHHSDRGIQYCSNAYQAVHAQHGVTCSMTDGYDCYQNALAERVNGILKQEFLTHKPATLEEARQMVAEAVAIYNQRRPHLALKYKTPDEVHRAF
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1217 bp | 405 aa | 53 | 1269 | + | Yes |
Chemistry : DDE
ORF sequence :
MTSTIKRTQRDYSLAFKLAVVDQVEKGELTYKQAQDKYGIQGCSTVLVWLRKHGKLDWSLGTPPTSMRGQLMTEPTTLTPEQKIKALEAELEDTRMKAAF
FEAIVEKLRTDYGISVVKKATRETLQEKTAIGYGVTQCCRYLGISRQAYYQQCQRQIVSELKEQEVVQTVQYERLFQPRVGTRKLQYLLNKMHQIVIGRD
RLFRCLRAHRLLVTPKRAYHKTTHSHHRFRCHPNLLKPSEQQVDISRSEQVWVADITYLSLQDNEAYLSLVTDAWSRKIVGYHVDDNLKTDGVLTAYKRA
LRQRQDKSKPLIHHSDRGIQYCSNAYQAVHAQHGVTCSMTDGYDCYQNALAERVNGILKQEFLTHKPATLEEARQMVAEAVAIYNQRRPHLALKYKTPDE
VHRAF
FEAIVEKLRTDYGISVVKKATRETLQEKTAIGYGVTQCCRYLGISRQAYYQQCQRQIVSELKEQEVVQTVQYERLFQPRVGTRKLQYLLNKMHQIVIGRD
RLFRCLRAHRLLVTPKRAYHKTTHSHHRFRCHPNLLKPSEQQVDISRSEQVWVADITYLSLQDNEAYLSLVTDAWSRKIVGYHVDDNLKTDGVLTAYKRA
LRQRQDKSKPLIHHSDRGIQYCSNAYQAVHAQHGVTCSMTDGYDCYQNALAERVNGILKQEFLTHKPATLEEARQMVAEAVAIYNQRRPHLALKYKTPDE
VHRAF
Blast result :
Comments
The functional ISShes13 transposase (Orf3) is predicted to be derived from a programmed frameshift of Orf1 and Orf2. There are 4 copies of ISShes13 on the chromosome. The ISShes13 transposase (Orf3) is 75% aa similar to ISVisp4_aa3.
References
1] Romine, M. F. (2008) Direct submission