ISSba14
- Family Tn3
- Group
Isoform Synonym(s) TnSba14
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009052 | ND | Shewanella baltica | Shewanella baltica OS155 |
DNA section
IS Length : 3815 bp
Ends
IR Length : 46/50
IRL : GGGGTCCGCTTGGAAAACGGAAAATATCCCACGCTAAGACCATTTTTTAA
IRR : GGGGTCCGCTTGGAAAACGGAAAATATCCCACGCTAAGACTGTTTTTTGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAAACTGTAC | TTAAA | CAGAAATATT | 5 |
| TTGACTTAAT | TATTAGGGGC | 0 | |
| AGACCACGTT | TGCTACAACA | 0 |
DNA sequence
GGGGTCCGCTTGGAAAACGGAAAATATCCCACGCTAAGACCATTTTTTAAACAGCAGGTATTTGCTGTTTTACTTTAACTCTCAGAATTGTTTGCCGTGT
GGTTCTGAATTCCCGGGCAATAGCACTTATACTTACACCAGACTGAATTCGTTCGAGTACCCTCTGTTTTTGTTCTTCATTTAACACCGGCGGACGCCCG
AAGCGTTTTCCTGCTGCCTTTGCCCTTGCAATACCAGAATGAGTGCGTTCAAGAAGCAGGTCCCGTTCAAACTCCGCAACCGCAGAAATCACCTGCATCG
TCATTTTTCCTGCAGGACTAGTCAAATCAACACCACCCAATGCCAAGCAATGCACTCGAATACCTGATCCGGCCAGTTGCTCCACTGTTTTTCTGATATC
CATCGCATTACGACCAAGACGATCCAATTTTGTGACAATCAGTACATCGCCGTTTTCCATGCGGTCAAGTAGCTTAGTGAACCCCGGACGCTCACCGGCA
GTTACTGAACCGCTGATCTGTTCTTCGAATAGTCGCTGGGGTTTGACAGCAAAACCTGCCTCTTCAATTTCACGGCGCTGATTTTCGGTGGTCTGCTCTA
GAGTGGATACACGGCAATAAGCAAAAACTCGTGACATAGCTAAATGTTTCGTACGAAAAGGGTGTACTAATTATAAGCCATGTACGAAATTATTTTAACT
AATATTCGTACATATTTAGAATAGGGTGTCCAAAACCGGTCAGTTTCGGTCAAAGTTAACCACTAACAAATTGAAACTATACGCGAGGGAAGCATTTATG
CCACGGCGACAGATACTAAGCAGTGAAGAACAGAAGCGCTTACTGGTTATACCAGATGATGAAATTATTCTGACCCGAATGTGTTTTTTGAATGAGCGTG
ATATTGCGCTCATAAATAAGCACAGACGGCCAGCGAATCGCCTGGGCTTTGCCGTTTTACTCTGCTATCTGCGTGGCTCCGGATTTATTCCGGACAAAAA
TACCTCGCCTCACTGTGGCGTCGTATCCAGAGTCGCTTCCCGATTGAAACTTCAGCCTGATTTATGGCATGAATATGCATCCAGAGAGCAAACTCGCTGG
GAACATCTGACCGAACTTTATCGTTACCTGAAATTATCCCCATTCAGCCGAACACTGCAAAAAGACTGCATCCGGCATCTGCACCCCTATGCCATGCGAA
CTGACAAAGGGTTTATGTTGGCGGAAGAAATGCTCAGCTGGCTACATAACAATAATGTTATTTTCCCTTCTGTTGATGTGATCGAGCGGACGATTGCCGA
AATCGTCACGCTGTCTGACAGAGCCGTATTTTCGGCACTTACCGCGCAACTGGAAAATCATCATAAATCAGCACTCGACAGCCTGCTCATATCAGAGAGT
GACCAACCTTCCCGTCTGGCGTGGTTACTTCAGCCATCGGGTAAAATCAACGGTAAAAATGTGCTGCAACATATCGACCGGCTTAATTCCATTACTGCGC
TGGGATTGCCTGATGGTATTGCGCTTTCCGTTCACCAGAACAGGTTGCTTAAGCTAGCGCGTGAGGGACGAAAAATGAGCAGCAGAGATCTGGCAAAATT
CACCGATGTCAGACGTTACGCTACGATGATTTGTATAATAACAGAGGCCCGGGCCACCCTAACAGACGAAGTGATTGATCTACACGAGCGTATTCTGGGC
AGTCTGTTCAGCAGGGCAAAACGAACGCAGTCCGAACGACTCCAGCAAACGGGAAAGCTTATTCAGAGCAAGCTAAAGCAGTACGTTACCGTCGGTCAGG
CGTTACTTAACGCCAGAGAATCCGGCGAAGATCCCTGGACCGCAATAGAAGACGTCCTACCATGGCAGGAATTCATCAACAGTGTGGAGGAAACGCGGGT
TCTGTCCCGTAAGGGCAATTTCGACCCACTTCACCTGATCACCGAAAAATACAGCACGCTGCGTAAATACGCCCCGCGTATGCTGTCAGCATTGCAGTTC
ACGGCGGCGCCTGCAGCTCAGATGCTCAGCGATGCACTGGACACCATAACTGAAATGTACCGTAAGCAACTTCGTCAAGTTCCGCCGGCAGCGCCAACAG
GATTTATCCCTGAAAGCTGGCGAAAACTGGTGCTCACACCATCCGGGATCGACCGCAAATACTACGAGTTTTGCGTACTGAATGAACTCAAAGGGGCGTT
ACGTTCCGGTGATATCTGGGTAAAAGGATCACGTCGCTACAGAAATTTTGATGATTATCTCATCACGGCTGCTGAGTTTGAGAAATTCCGCCATAATGAT
CAGTTACAGTTGGCCGTTCAGACCGATTGCCGGGCATACCTTCAGGCTCGTATGACCCTTCTTGCATCGCGTCTGGAAGAAGTTAACGCGATGGCGCTTG
CTGGTGATTTGCCCGATGTTGATATCTCAGATAAAGGCGTAAAAATAACCCCGTTGGAGAACAGCGTTCCTTCGGGTGTCTCGCCCTTTGCCGATTTGGT
TTATGGCATGCTTCCCCATCCGAAAATTACGGAGATACTGGAAGAAGTGGATAACTGGACTGGGTTTACGCGTCACTTCACGCACCTCAAAAATAATAAC
GTCAGACCAAAAGACGGAAGACCGTTGCTGACCACCATTTTGGCTGACGGCATCAATCTGGGGCTGACAAAAATGGCGGAGTCCTGCCCGGGGGCAACAA
AATCGACACTCGAAGGTATTCAGGCATGGTACATCAGAGATGAAACTTATTCAGCAGCACTTGTTGAGTTGGTCAATGCCCAGAAAGCGCGCCCGCTAGC
CGCATTCTGGGGTGATGGTACAACATCGTCGTCAGACGGACAGAACTTTCGGGTAGGCAGTCACGGACGTTATGCCGGTCAGGTCAATCTTAAATATGGT
CAAGAACCGGGCGTGCAGATTTATACGCATATCTCAGATCAATACAGTCCGTACTACGCCAAAGTGATCACTCGGGTGCGCGACTCAACTCACGTGCTTG
ATGGCCTTCTGTACCATGAAAGCGACTTGGAAATTACCGAGCATTACACCGATACAGCAGGCTTTACAGAACATGTTTTTGCCCTGATGCATCTGCTGGG
ATTCACTTTTGCGCCAAGGATCCGGGATCTTCATGACAAGCGGTTGTTTATTCAAGGAAATAACTGGGATTATCCGGGGCTTCAGTCCGTTATATCAACA
ACCCGCCTGAATCTCAAAGACATTGAGGCGCACTGGGATGAAGTATTACGGCTAGCAACTTCGATAAAGCAGGGGACAGTCACCGCATCGCTGATGATGA
AAAAGTTAGCCAGTTATCCAAAACAGAACGGACTTGCCAAAGCGCTGAGAGAGATAGGTCGAATAGAGCGGACACTGTTTATGCTGGACTGGTTTCGAGA
TCCGGGTCTGCGGCAACGGGTACAGGCTGGGCTGAATAAGGGTGAGGCCCGTAATGCCCTTGCGCGTGCGGTTTTTATGCATCGTCTGGGTGAAATCAGA
GATCGGGGGCTGGAAAATCAGAGTTACCGTGCCAGCGGACTGACGTTACTGACAGCGGCGATCACGTTATGGAACACGGTATATATAGAAAGAGCCATTG
AGTCACTGAAACGAAAAGGTGTCCCTATTAATGAACAACTAGTTGCTCATCTTTCTCCCCTGGGCTGGGAACATATTAATCTGAGTGGCGATTACGTTTG
GCGCCATAATGTAAAGCTGGGGTCCGGAAAATATCGGCCATTACGTACAGTCAATACTGAATTGTACAAAAAACAGTCTTAGCGTGGGATATTTTCCGTT
TTCCAAGCGGACCCC
GGTTCTGAATTCCCGGGCAATAGCACTTATACTTACACCAGACTGAATTCGTTCGAGTACCCTCTGTTTTTGTTCTTCATTTAACACCGGCGGACGCCCG
AAGCGTTTTCCTGCTGCCTTTGCCCTTGCAATACCAGAATGAGTGCGTTCAAGAAGCAGGTCCCGTTCAAACTCCGCAACCGCAGAAATCACCTGCATCG
TCATTTTTCCTGCAGGACTAGTCAAATCAACACCACCCAATGCCAAGCAATGCACTCGAATACCTGATCCGGCCAGTTGCTCCACTGTTTTTCTGATATC
CATCGCATTACGACCAAGACGATCCAATTTTGTGACAATCAGTACATCGCCGTTTTCCATGCGGTCAAGTAGCTTAGTGAACCCCGGACGCTCACCGGCA
GTTACTGAACCGCTGATCTGTTCTTCGAATAGTCGCTGGGGTTTGACAGCAAAACCTGCCTCTTCAATTTCACGGCGCTGATTTTCGGTGGTCTGCTCTA
GAGTGGATACACGGCAATAAGCAAAAACTCGTGACATAGCTAAATGTTTCGTACGAAAAGGGTGTACTAATTATAAGCCATGTACGAAATTATTTTAACT
AATATTCGTACATATTTAGAATAGGGTGTCCAAAACCGGTCAGTTTCGGTCAAAGTTAACCACTAACAAATTGAAACTATACGCGAGGGAAGCATTTATG
CCACGGCGACAGATACTAAGCAGTGAAGAACAGAAGCGCTTACTGGTTATACCAGATGATGAAATTATTCTGACCCGAATGTGTTTTTTGAATGAGCGTG
ATATTGCGCTCATAAATAAGCACAGACGGCCAGCGAATCGCCTGGGCTTTGCCGTTTTACTCTGCTATCTGCGTGGCTCCGGATTTATTCCGGACAAAAA
TACCTCGCCTCACTGTGGCGTCGTATCCAGAGTCGCTTCCCGATTGAAACTTCAGCCTGATTTATGGCATGAATATGCATCCAGAGAGCAAACTCGCTGG
GAACATCTGACCGAACTTTATCGTTACCTGAAATTATCCCCATTCAGCCGAACACTGCAAAAAGACTGCATCCGGCATCTGCACCCCTATGCCATGCGAA
CTGACAAAGGGTTTATGTTGGCGGAAGAAATGCTCAGCTGGCTACATAACAATAATGTTATTTTCCCTTCTGTTGATGTGATCGAGCGGACGATTGCCGA
AATCGTCACGCTGTCTGACAGAGCCGTATTTTCGGCACTTACCGCGCAACTGGAAAATCATCATAAATCAGCACTCGACAGCCTGCTCATATCAGAGAGT
GACCAACCTTCCCGTCTGGCGTGGTTACTTCAGCCATCGGGTAAAATCAACGGTAAAAATGTGCTGCAACATATCGACCGGCTTAATTCCATTACTGCGC
TGGGATTGCCTGATGGTATTGCGCTTTCCGTTCACCAGAACAGGTTGCTTAAGCTAGCGCGTGAGGGACGAAAAATGAGCAGCAGAGATCTGGCAAAATT
CACCGATGTCAGACGTTACGCTACGATGATTTGTATAATAACAGAGGCCCGGGCCACCCTAACAGACGAAGTGATTGATCTACACGAGCGTATTCTGGGC
AGTCTGTTCAGCAGGGCAAAACGAACGCAGTCCGAACGACTCCAGCAAACGGGAAAGCTTATTCAGAGCAAGCTAAAGCAGTACGTTACCGTCGGTCAGG
CGTTACTTAACGCCAGAGAATCCGGCGAAGATCCCTGGACCGCAATAGAAGACGTCCTACCATGGCAGGAATTCATCAACAGTGTGGAGGAAACGCGGGT
TCTGTCCCGTAAGGGCAATTTCGACCCACTTCACCTGATCACCGAAAAATACAGCACGCTGCGTAAATACGCCCCGCGTATGCTGTCAGCATTGCAGTTC
ACGGCGGCGCCTGCAGCTCAGATGCTCAGCGATGCACTGGACACCATAACTGAAATGTACCGTAAGCAACTTCGTCAAGTTCCGCCGGCAGCGCCAACAG
GATTTATCCCTGAAAGCTGGCGAAAACTGGTGCTCACACCATCCGGGATCGACCGCAAATACTACGAGTTTTGCGTACTGAATGAACTCAAAGGGGCGTT
ACGTTCCGGTGATATCTGGGTAAAAGGATCACGTCGCTACAGAAATTTTGATGATTATCTCATCACGGCTGCTGAGTTTGAGAAATTCCGCCATAATGAT
CAGTTACAGTTGGCCGTTCAGACCGATTGCCGGGCATACCTTCAGGCTCGTATGACCCTTCTTGCATCGCGTCTGGAAGAAGTTAACGCGATGGCGCTTG
CTGGTGATTTGCCCGATGTTGATATCTCAGATAAAGGCGTAAAAATAACCCCGTTGGAGAACAGCGTTCCTTCGGGTGTCTCGCCCTTTGCCGATTTGGT
TTATGGCATGCTTCCCCATCCGAAAATTACGGAGATACTGGAAGAAGTGGATAACTGGACTGGGTTTACGCGTCACTTCACGCACCTCAAAAATAATAAC
GTCAGACCAAAAGACGGAAGACCGTTGCTGACCACCATTTTGGCTGACGGCATCAATCTGGGGCTGACAAAAATGGCGGAGTCCTGCCCGGGGGCAACAA
AATCGACACTCGAAGGTATTCAGGCATGGTACATCAGAGATGAAACTTATTCAGCAGCACTTGTTGAGTTGGTCAATGCCCAGAAAGCGCGCCCGCTAGC
CGCATTCTGGGGTGATGGTACAACATCGTCGTCAGACGGACAGAACTTTCGGGTAGGCAGTCACGGACGTTATGCCGGTCAGGTCAATCTTAAATATGGT
CAAGAACCGGGCGTGCAGATTTATACGCATATCTCAGATCAATACAGTCCGTACTACGCCAAAGTGATCACTCGGGTGCGCGACTCAACTCACGTGCTTG
ATGGCCTTCTGTACCATGAAAGCGACTTGGAAATTACCGAGCATTACACCGATACAGCAGGCTTTACAGAACATGTTTTTGCCCTGATGCATCTGCTGGG
ATTCACTTTTGCGCCAAGGATCCGGGATCTTCATGACAAGCGGTTGTTTATTCAAGGAAATAACTGGGATTATCCGGGGCTTCAGTCCGTTATATCAACA
ACCCGCCTGAATCTCAAAGACATTGAGGCGCACTGGGATGAAGTATTACGGCTAGCAACTTCGATAAAGCAGGGGACAGTCACCGCATCGCTGATGATGA
AAAAGTTAGCCAGTTATCCAAAACAGAACGGACTTGCCAAAGCGCTGAGAGAGATAGGTCGAATAGAGCGGACACTGTTTATGCTGGACTGGTTTCGAGA
TCCGGGTCTGCGGCAACGGGTACAGGCTGGGCTGAATAAGGGTGAGGCCCGTAATGCCCTTGCGCGTGCGGTTTTTATGCATCGTCTGGGTGAAATCAGA
GATCGGGGGCTGGAAAATCAGAGTTACCGTGCCAGCGGACTGACGTTACTGACAGCGGCGATCACGTTATGGAACACGGTATATATAGAAAGAGCCATTG
AGTCACTGAAACGAAAAGGTGTCCCTATTAATGAACAACTAGTTGCTCATCTTTCTCCCCTGGGCTGGGAACATATTAATCTGAGTGGCGATTACGTTTG
GCGCCATAATGTAAAGCTGGGGTCCGGAAAATATCGGCCATTACGTACAGTCAATACTGAATTGTACAAAAAACAGTCTTAGCGTGGGATATTTTCCGTT
TTCCAAGCGGACCCC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 591 bp | 196 aa | 47 | 637 | - | No |
AG : Tn3 resolvase
ORF sequence :
MSRVFAYCRVSTLEQTTENQRREIEEAGFAVKPQRLFEEQISGSVTAGERPGFTKLLDRMENGDVLIVTKLDRLGRNAMDIRKTVEQLAGSGIRVHCLAL
GGVDLTSPAGKMTMQVISAVAEFERDLLLERTHSGIARAKAAGKRFGRPPVLNEEQKQRVLERIQSGVSISAIAREFRTTRQTILRVKVKQQIPAV
GGVDLTSPAGKMTMQVISAVAEFERDLLLERTHSGIARAKAAGKRFGRPPVLNEEQKQRVLERIQSGVSISAIAREFRTTRQTILRVKVKQQIPAV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 2985 bp | 994 aa | 798 | 3782 | + | No |
Chemistry : DDE
ORF sequence :
MPRRQILSSEEQKRLLVIPDDEIILTRMCFLNERDIALINKHRRPANRLGFAVLLCYLRGSGFIPDKNTSPHCGVVSRVASRLKLQPDLWHEYASREQTR
WEHLTELYRYLKLSPFSRTLQKDCIRHLHPYAMRTDKGFMLAEEMLSWLHNNNVIFPSVDVIERTIAEIVTLSDRAVFSALTAQLENHHKSALDSLLISE
SDQPSRLAWLLQPSGKINGKNVLQHIDRLNSITALGLPDGIALSVHQNRLLKLAREGRKMSSRDLAKFTDVRRYATMICIITEARATLTDEVIDLHERIL
GSLFSRAKRTQSERLQQTGKLIQSKLKQYVTVGQALLNARESGEDPWTAIEDVLPWQEFINSVEETRVLSRKGNFDPLHLITEKYSTLRKYAPRMLSALQ
FTAAPAAQMLSDALDTITEMYRKQLRQVPPAAPTGFIPESWRKLVLTPSGIDRKYYEFCVLNELKGALRSGDIWVKGSRRYRNFDDYLITAAEFEKFRHN
DQLQLAVQTDCRAYLQARMTLLASRLEEVNAMALAGDLPDVDISDKGVKITPLENSVPSGVSPFADLVYGMLPHPKITEILEEVDNWTGFTRHFTHLKNN
NVRPKDGRPLLTTILADGINLGLTKMAESCPGATKSTLEGIQAWYIRDETYSAALVELVNAQKARPLAAFWGDGTTSSSDGQNFRVGSHGRYAGQVNLKY
GQEPGVQIYTHISDQYSPYYAKVITRVRDSTHVLDGLLYHESDLEITEHYTDTAGFTEHVFALMHLLGFTFAPRIRDLHDKRLFIQGNNWDYPGLQSVIS
TTRLNLKDIEAHWDEVLRLATSIKQGTVTASLMMKKLASYPKQNGLAKALREIGRIERTLFMLDWFRDPGLRQRVQAGLNKGEARNALARAVFMHRLGEI
RDRGLENQSYRASGLTLLTAAITLWNTVYIERAIESLKRKGVPINEQLVAHLSPLGWEHINLSGDYVWRHNVKLGSGKYRPLRTVNTELYKKQS
WEHLTELYRYLKLSPFSRTLQKDCIRHLHPYAMRTDKGFMLAEEMLSWLHNNNVIFPSVDVIERTIAEIVTLSDRAVFSALTAQLENHHKSALDSLLISE
SDQPSRLAWLLQPSGKINGKNVLQHIDRLNSITALGLPDGIALSVHQNRLLKLAREGRKMSSRDLAKFTDVRRYATMICIITEARATLTDEVIDLHERIL
GSLFSRAKRTQSERLQQTGKLIQSKLKQYVTVGQALLNARESGEDPWTAIEDVLPWQEFINSVEETRVLSRKGNFDPLHLITEKYSTLRKYAPRMLSALQ
FTAAPAAQMLSDALDTITEMYRKQLRQVPPAAPTGFIPESWRKLVLTPSGIDRKYYEFCVLNELKGALRSGDIWVKGSRRYRNFDDYLITAAEFEKFRHN
DQLQLAVQTDCRAYLQARMTLLASRLEEVNAMALAGDLPDVDISDKGVKITPLENSVPSGVSPFADLVYGMLPHPKITEILEEVDNWTGFTRHFTHLKNN
NVRPKDGRPLLTTILADGINLGLTKMAESCPGATKSTLEGIQAWYIRDETYSAALVELVNAQKARPLAAFWGDGTTSSSDGQNFRVGSHGRYAGQVNLKY
GQEPGVQIYTHISDQYSPYYAKVITRVRDSTHVLDGLLYHESDLEITEHYTDTAGFTEHVFALMHLLGFTFAPRIRDLHDKRLFIQGNNWDYPGLQSVIS
TTRLNLKDIEAHWDEVLRLATSIKQGTVTASLMMKKLASYPKQNGLAKALREIGRIERTLFMLDWFRDPGLRQRVQAGLNKGEARNALARAVFMHRLGEI
RDRGLENQSYRASGLTLLTAAITLWNTVYIERAIESLKRKGVPINEQLVAHLSPLGWEHINLSGDYVWRHNVKLGSGKYRPLRTVNTELYKKQS
Blast result :
Comments
There are 3 copies of ISSba14 encoded in the chromosome (1 truncated, one having in-frame stop codon in transposase) and 2 copies in plasmid 2 (1 truncated) of Shewanella baltica OS155. ISSba14 encodes both a resolvase and transposase. The transposase has 54% identity to ISSod9_aa4.
References
1] Romine, M. F. (2008) Direct submission