ISShes12
- Family IS1634
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008573 | ND | Shewanella sp. | Shewanella sp. ANA-3 |
DNA section
IS Length : 1614 bp
Ends
IR Length : 20/25
IRL : CCGTCATTCCGCACCTAATTTTCGATTTAAAAAACTTGACGGCGATCGGT
IRR : CCGACTTTCCGCACCTGATTTAGGAATAAATTTGCCGATACCGTTCGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGACTTACCT | ATTTAA | ATTATTCCAT | 6 |
| ACAGGCAGTT | ACAGAT | GGCTATACAA | 6 |
| AATGGGAGTA | CTGAAA | GGAATCAGTA | 6 |
| CATATTTGCT | TTAAAT | ATGAAGTGAA | 6 |
| GCAATAACGC | A/TTCCAT | TCCTTCATAG | 8 |
| CTGCTTCCAG | TTGTAA | GGAAGTGAGC | 6 |
| AAGTAATACA | ATAAAT | AGGAAGAAAA | 6 |
| TGCCATTAAA | TTAAAT | GCAAATTAAC | 6 |
| ACAATAACTG | TTGAAA | CCGGTAAAAC | 6 |
DNA sequence
CCGTCATTCCGCACCTAATTTTCGATTTAAAAAACTTGACGGCGATCGGTCATTCTGATCTATTACTCTCTTTTGCAGGTAATTCATCATGGCCGCTCCC
GCTATCTCTATCCGTAACCTCGACCACCTTGGTTTGGTCGCTGCACTTTGCCAAGAACTCGGCATTGCTCGCATGATTGATGCGGTGCTACCCAAAACGC
CGCCCTACAAAGTCTCACATGGTGAAGCGTTGGTGGCGATGATACTGAACGGTCTTGGCTTTCATAGTTCGACCTTGCACATGTTCCCGCAATTCTTTGC
CAACAAGCCTGTAGAACGCCTGATTGGCCCGGGGATCTGTGCCGATGACCTCAATGATGATGTGCTCGGCCGCTGCCTTGATGCCCTCTTTGAGGCCGAT
GTCAGCACGCTTTATCAGGTGATGGCGGAGCAGGTCGTTGAACGATTGGGGCTCAAAAGTACTGCAGTACACCTCGATATCACCAGCTTCCACGTCGATG
GCGCCTATGACCAAGCCGATGGCGAGCAGCTCGGTAAGTTGCAATTGGTACGGGGCTATAGCCGCGACCATCGGCCAGAGCTAAACCAGGTTATCCTGGA
GCTTATCTGTGAAAATCAGGCCGGCCTGCCCATCTACATGCAAGCCTTGAGTGGTAATATTAATGATACCAAGGCCTTTGCCCAGACGGTACGGCGCCAC
TTGAGCAGTCTCAAGGCCGCGCAGGAGAGTCGTTATCTGGTCGGCGATGCGGCGCTGTATTGCGCCGACACCTTGCAACTACTGGCACAACAGCAACAGT
TGTTCGTGACTCGTGTGCCTGTCACCCTCAATGAAGCCAAGCGAGCCGTGGCAACCATTGGCACCCAGTCGCTGGTTGCGCTGGGCAATGGCTATCATGG
CTGTTGGCAAAGCGCCAATTATGCCGACGTCGCGCAGCGTTGGTTGCTGGTGCGCAGCGAACAGGCCAGCCACCGTGAGCAGCAAACATTAGCCAAAAAC
CTACTGAAAGACAGCACGCGTGAACTGAAAGCCTTCAACAAATTATGTGCCCAGCGTTTTGCCTGCGAGACCGATGCGCAGGCTGAATTAGCCCGTTTTG
CTGTCAGCTTAAATCTGCTGCAACTAGAAGCAGAAGTCGTGAGTGAACCTATCTATGCAGGGCGCGGAAGGCCCCAAAAGGGTGAAATGCCGGTGAACTA
CCAATTTCACATCAAGGGGTTAGCCGCAAGTAGCTTGGCGCGGGTGGCTGAGGCGCGCAACCAGACAGGGGTGTTCATCCTCGCGACCAATGATCACAGT
GAAACGCTCACCATGGCTGAGCTGTTGGCTACCTATAAAGCCCAGCAGAATGTCGAGCGTGGTTTTAGGTTTCTGAAAAGTCCCGAATTTTTAACCTCCT
CGTTATATCTTAAAAAACCGGAACGTATCGAAGCGTTGCTGATGGTGATGACCTGTAGCCTGATGATTTATGCGGCGCTGGAGCACCGGATCCGGCAGGG
CTTAGTAGAGCAGAACCGGAGTGTGGCGGACATGAAAAAGAAACCCACGCAAAAACCGACTGCGCGGTGGATTTTTTTACGCTTTGGCGGGGTGCACGAG
TACCAGTTGGGAGAGGCGCCACCTCAAGTCACAGGACTCACAGTGGACCAGCAGATCATTCTCGACGTGTTAGGCGAACGGTATCGGCAAATTTATTCCT
AAATCAGGTGCGGAAAGTCGG
GCTATCTCTATCCGTAACCTCGACCACCTTGGTTTGGTCGCTGCACTTTGCCAAGAACTCGGCATTGCTCGCATGATTGATGCGGTGCTACCCAAAACGC
CGCCCTACAAAGTCTCACATGGTGAAGCGTTGGTGGCGATGATACTGAACGGTCTTGGCTTTCATAGTTCGACCTTGCACATGTTCCCGCAATTCTTTGC
CAACAAGCCTGTAGAACGCCTGATTGGCCCGGGGATCTGTGCCGATGACCTCAATGATGATGTGCTCGGCCGCTGCCTTGATGCCCTCTTTGAGGCCGAT
GTCAGCACGCTTTATCAGGTGATGGCGGAGCAGGTCGTTGAACGATTGGGGCTCAAAAGTACTGCAGTACACCTCGATATCACCAGCTTCCACGTCGATG
GCGCCTATGACCAAGCCGATGGCGAGCAGCTCGGTAAGTTGCAATTGGTACGGGGCTATAGCCGCGACCATCGGCCAGAGCTAAACCAGGTTATCCTGGA
GCTTATCTGTGAAAATCAGGCCGGCCTGCCCATCTACATGCAAGCCTTGAGTGGTAATATTAATGATACCAAGGCCTTTGCCCAGACGGTACGGCGCCAC
TTGAGCAGTCTCAAGGCCGCGCAGGAGAGTCGTTATCTGGTCGGCGATGCGGCGCTGTATTGCGCCGACACCTTGCAACTACTGGCACAACAGCAACAGT
TGTTCGTGACTCGTGTGCCTGTCACCCTCAATGAAGCCAAGCGAGCCGTGGCAACCATTGGCACCCAGTCGCTGGTTGCGCTGGGCAATGGCTATCATGG
CTGTTGGCAAAGCGCCAATTATGCCGACGTCGCGCAGCGTTGGTTGCTGGTGCGCAGCGAACAGGCCAGCCACCGTGAGCAGCAAACATTAGCCAAAAAC
CTACTGAAAGACAGCACGCGTGAACTGAAAGCCTTCAACAAATTATGTGCCCAGCGTTTTGCCTGCGAGACCGATGCGCAGGCTGAATTAGCCCGTTTTG
CTGTCAGCTTAAATCTGCTGCAACTAGAAGCAGAAGTCGTGAGTGAACCTATCTATGCAGGGCGCGGAAGGCCCCAAAAGGGTGAAATGCCGGTGAACTA
CCAATTTCACATCAAGGGGTTAGCCGCAAGTAGCTTGGCGCGGGTGGCTGAGGCGCGCAACCAGACAGGGGTGTTCATCCTCGCGACCAATGATCACAGT
GAAACGCTCACCATGGCTGAGCTGTTGGCTACCTATAAAGCCCAGCAGAATGTCGAGCGTGGTTTTAGGTTTCTGAAAAGTCCCGAATTTTTAACCTCCT
CGTTATATCTTAAAAAACCGGAACGTATCGAAGCGTTGCTGATGGTGATGACCTGTAGCCTGATGATTTATGCGGCGCTGGAGCACCGGATCCGGCAGGG
CTTAGTAGAGCAGAACCGGAGTGTGGCGGACATGAAAAAGAAACCCACGCAAAAACCGACTGCGCGGTGGATTTTTTTACGCTTTGGCGGGGTGCACGAG
TACCAGTTGGGAGAGGCGCCACCTCAAGTCACAGGACTCACAGTGGACCAGCAGATCATTCTCGACGTGTTAGGCGAACGGTATCGGCAAATTTATTCCT
AAATCAGGTGCGGAAAGTCGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1614 bp | 537 aa | 89 | 1702 | + | No |
Chemistry : DDE
ORF sequence :
MAAPAISIRNLDHLGLVAALCQELGIARMIDAVLPKTPPYKVSHGEALVAMILNGLGFHSSTLHMFPQFFANKPVERLIGPGICADDLNDDVLGRCLDAL
FEADVSTLYQVMAEQVVERLGLKSTAVHLDITSFHVDGAYDQADGEQLGKLQLVRGYSRDHRPELNQVILELICENQAGLPIYMQALSGNINDTKAFAQT
VRRHLSSLKAAQESRYLVGDAALYCADTLQLLAQQQQLFVTRVPVTLNEAKRAVATIGTQSLVALGNGYHGCWQSANYADVAQRWLLVRSEQASHREQQT
LAKNLLKDSTRELKAFNKLCAQRFACETDAQAELARFAVSLNLLQLEAEVVSEPIYAGRGRPQKGEMPVNYQFHIKGLAASSLARVAEARNQTGVFILAT
NDHSETLTMAELLATYKAQQNVERGFRFLKSPEFLTSSLYLKKPERIEALLMVMTCSLMIYAALEHRIRQGLVEQNRSVADMKKKPTQKPTARWIFLRFG
GVHEYQLGEAPPQVTGLTVDQQIILDVLGERYRQIYS
FEADVSTLYQVMAEQVVERLGLKSTAVHLDITSFHVDGAYDQADGEQLGKLQLVRGYSRDHRPELNQVILELICENQAGLPIYMQALSGNINDTKAFAQT
VRRHLSSLKAAQESRYLVGDAALYCADTLQLLAQQQQLFVTRVPVTLNEAKRAVATIGTQSLVALGNGYHGCWQSANYADVAQRWLLVRSEQASHREQQT
LAKNLLKDSTRELKAFNKLCAQRFACETDAQAELARFAVSLNLLQLEAEVVSEPIYAGRGRPQKGEMPVNYQFHIKGLAASSLARVAEARNQTGVFILAT
NDHSETLTMAELLATYKAQQNVERGFRFLKSPEFLTSSLYLKKPERIEALLMVMTCSLMIYAALEHRIRQGLVEQNRSVADMKKKPTQKPTARWIFLRFG
GVHEYQLGEAPPQVTGLTVDQQIILDVLGERYRQIYS
Blast result :
Comments
There are 9 copies of ISShes12 encoded in the Shewanella sp. ANA-3 chromosome. All copies are full length, but one has a single bp mutation in the flanking direct repeat. The ISShes12 transposase has 55% identity to that of ISPlu4.
References
1] Romine, M.F. (2008). Direct submission.