ISShes10
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008573 | ND | Shewanella sp. | Shewanella sp. ANA-3 |
DNA section
IS Length : 1113 bp
Ends
IR Length : 20/27
IRL : GGCGGCCGCAAAAACTGAGTGCAACACCTGACCTTTCCGGTACGGTGCTG
IRR : GCTGGTTGCAGGATCTGAGTGCAACACGGTTATTGAATTGAAGCGGGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATCACGCAC | TATTATGATG | 0 |
DNA sequence
GGCGGCCGCAAAAACTGAGTGCAACACCTGACCTTTCCGGTACGGTGCTGCCACTATGTCCTATCACGAACTCAGTATCCCCGAACGTGCGACCCTTCAA
CTCGGCCTTGCCCAAGGTTTCAGCCAGCGGCGAATTGCCCGGATGCTCGGCCGCTCCCCCTCGACCATCAGCCGGGAGCTGCGCCGCAACCGGGCCCCTG
GCACCCGCTACCAGGCCTGTGCTGCCCAGCAGCAGATGCAGGTCCGCCGCCAGGTCTGCAGGCCTCAGTGCAAGCTGCTGCCGGGCAGTGAGCGCTTCGA
GCTGGTCGTCCATATGCTGCGTGAGGGTTTGTCTCCCGAGCAGATTGCCGGCAAGCTGCGCAGCATGAACATACCCAGCCTCAGAGAGGCCTACGTCTGC
CGCGAGACGATCTATAACGCGATCTACGCCCTGCCGGTCGGTGGCCTGCGCAAGGAGCTGATCATCTGCCTGCGCCAGGGCAAGACGAGTCGCAGGCCGC
GCTCTGGCGGCGTCGATCGGCGCGGCCAGATCCCTGAAATGCTCAGCATCCACGTGCGCCCGCCGGAGGTCGAAGACCGGCTGATGCCAGGCCATTGGGA
GGGCGACCTGATCAAGGGCAAGGCCAATGCCTCGGCGGTGGGCACCTTGGTGGAGCGTACCAGCGGCTACCTGATGCTGATCAAGATGGCCGACGCGACG
GCGACCTCGGCGCTGGAGGGCTTCAGTGCGGCGCTCAATGGCATGCCGCTGGCGGTGCGCAAGAGCATGACCTACGACCAGGGCCGGGAGATGGCGCGGC
ACGCCGAGATCACCCAGCGGACCGGGGTGGCGATCTACTTCTGCGACCCGCACAGCCCCTGGCAACGCGGCAGCAATGAGAACATCAATGGACTGATCCG
CCAGTACCTGCCCAAGGGCACGGACTTGTCGGTGCATAGTCAGGAAGCCCTGGACGCCATCGCTTTGCAACTGAATATGCGACCGCGCAAACGCTTCGAC
TTCAAATGCCCGATCGAGGTGATGGGCGAAGTGATGCAAGAAGCCGTGGTGATGCGGCATGATGCTCCCGCTTCAATTCAATAACCGTGTTGCACTCAGA
TCCTGCAACCAGC
CTCGGCCTTGCCCAAGGTTTCAGCCAGCGGCGAATTGCCCGGATGCTCGGCCGCTCCCCCTCGACCATCAGCCGGGAGCTGCGCCGCAACCGGGCCCCTG
GCACCCGCTACCAGGCCTGTGCTGCCCAGCAGCAGATGCAGGTCCGCCGCCAGGTCTGCAGGCCTCAGTGCAAGCTGCTGCCGGGCAGTGAGCGCTTCGA
GCTGGTCGTCCATATGCTGCGTGAGGGTTTGTCTCCCGAGCAGATTGCCGGCAAGCTGCGCAGCATGAACATACCCAGCCTCAGAGAGGCCTACGTCTGC
CGCGAGACGATCTATAACGCGATCTACGCCCTGCCGGTCGGTGGCCTGCGCAAGGAGCTGATCATCTGCCTGCGCCAGGGCAAGACGAGTCGCAGGCCGC
GCTCTGGCGGCGTCGATCGGCGCGGCCAGATCCCTGAAATGCTCAGCATCCACGTGCGCCCGCCGGAGGTCGAAGACCGGCTGATGCCAGGCCATTGGGA
GGGCGACCTGATCAAGGGCAAGGCCAATGCCTCGGCGGTGGGCACCTTGGTGGAGCGTACCAGCGGCTACCTGATGCTGATCAAGATGGCCGACGCGACG
GCGACCTCGGCGCTGGAGGGCTTCAGTGCGGCGCTCAATGGCATGCCGCTGGCGGTGCGCAAGAGCATGACCTACGACCAGGGCCGGGAGATGGCGCGGC
ACGCCGAGATCACCCAGCGGACCGGGGTGGCGATCTACTTCTGCGACCCGCACAGCCCCTGGCAACGCGGCAGCAATGAGAACATCAATGGACTGATCCG
CCAGTACCTGCCCAAGGGCACGGACTTGTCGGTGCATAGTCAGGAAGCCCTGGACGCCATCGCTTTGCAACTGAATATGCGACCGCGCAAACGCTTCGAC
TTCAAATGCCCGATCGAGGTGATGGGCGAAGTGATGCAAGAAGCCGTGGTGATGCGGCATGATGCTCCCGCTTCAATTCAATAACCGTGTTGCACTCAGA
TCCTGCAACCAGC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1029 bp | 342 aa | 56 | 1084 | + | No |
Chemistry : DDE
ORF sequence :
MSYHELSIPERATLQLGLAQGFSQRRIARMLGRSPSTISRELRRNRAPGTRYQACAAQQQMQVRRQVCRPQCKLLPGSERFELVVHMLREGLSPEQIAGK
LRSMNIPSLREAYVCRETIYNAIYALPVGGLRKELIICLRQGKTSRRPRSGGVDRRGQIPEMLSIHVRPPEVEDRLMPGHWEGDLIKGKANASAVGTLVE
RTSGYLMLIKMADATATSALEGFSAALNGMPLAVRKSMTYDQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEALD
AIALQLNMRPRKRFDFKCPIEVMGEVMQEAVVMRHDAPASIQ
LRSMNIPSLREAYVCRETIYNAIYALPVGGLRKELIICLRQGKTSRRPRSGGVDRRGQIPEMLSIHVRPPEVEDRLMPGHWEGDLIKGKANASAVGTLVE
RTSGYLMLIKMADATATSALEGFSAALNGMPLAVRKSMTYDQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEALD
AIALQLNMRPRKRFDFKCPIEVMGEVMQEAVVMRHDAPASIQ
Blast result :
Comments
There is one copy of ISShes10 on the Shewanella sp. ANA-3 plasmid. The ISShes10 transposase has 87% identity with ISUnCu4. The ends of the IS element were approximated by comparison to the ISUnCu4 ends.
References
1] Romine, M. F. (2008). Direct submission.