ISShfr9
- Family Tn3
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008345 | ND | Shewanella frigidimarina | Shewanella frigidimarina NCBM4000 |
DNA section
IS Length : 3213 bp
Ends
IR Length : 76/81
IRL : GGGGTTCTGGGGATTTCCCCCTCTAAAAAGACATAATCCTCTGTAGACCA
IRR : GGGGTTCTGGGGATTTCCCCCTCTAAAAAGACATAATCCTCTGTAGACTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCGACCAATAT | TACATGCGTTA | 0 | |
| ATGCCTGAAT | AAAGTGCTTC | 0 |
DNA sequence
GGGGTTCTGGGGATTTCCCCCTCTAAAAAGACATAATCCTCTGTAGACCACACTAATAAATGGCTGTGGAGGTAGGTTACTACTTATAAGTGTGGCAAAT
TAGGTAGATATCGTACATTTTAATACGGAACAGCCCTGATATGGCCATCAAAAACGAAATTTCTATTCTCACGAGAGCAGAACAGGCAGATCTTTATTCC
CCACCCATTTTTTCAATCGAAGAACAACGTCTGTATTTTTCTCTGAACGATGGGGAATTGGCAGTTTTTCGGTCAATTCGTCTCAGAGCTCATAGATGTT
ACTTTGTCGCGATTTTGGGATACTTTAAATCAAAGCCCGTCATCCTAGATATCGCTTACTCGCAGGTTTCTAAGGATTTAATGTTCATCAGTAAAGAGCT
GCTTGGCGGCAAGGGGCTCAGACCATTCACTCCCTCACAAAAACAAAAAGATCGACTCTACGCAAAAGTATTAGACCTTGCTGGTTATCACAAATGGGAC
GAAAGTCAGCACTTCAATTCTCTTTTCGACCACCTTGTTCAGGTGGGCAATGCCTGGCTGGAGCCGCGTTACCTCTTTGATACTGCTATTGAATTCCTAG
CCAGTCACAGCATTGCTATCCCCAGGTATACCGTACTCCAGAGACTGATAAGCAGAACAATGCAGCAGGTCAGAAAAGACCTGGCGCACCAACTTAATCA
ACTCACCAGTCCTGAACTTCACGTCTTTCTGGACAGCATAACAGCCATTGATGACGGACTAAGCCTGAACCAGCTCAGAGGCGGTGCAAAAAGTCTGACC
GTACCTGAGCTTAAAAAAGAGCTTGCCCTTTATCATCAGTTAGCGCCATGGCGCACGCAAATCAATGGCGTTATCGATGGGCTTAATCTGTCTCTTAAAA
ATCGACAACACTTCGGTGAGCTCATCAACTATTACGGCAGTAAACTCAAACGATTCAAACGCGCACAGCAGCATCTATGGTTGCTATGTCACCTGACAGA
GCGGATACAACTGGCACTGGAACGGTTAACTGATGGGTTCATTTACCATATCCGCAAGCAACAAGAAGCTGCCAACACCTTTGCACAACAAGCAGTGTTC
CTGTCCTGGCAGTCAGCCGCGGACAATGTCACAAAAGCGGCAGAGTTACTGCATCTGTTTGTGGATGAGAACATTGATGATAATCAACCCTTCTCAGTAG
TCAGACAACAGGCATTGAAGGTCATGAATGACAGGGATATCCAGACCCTCTGCCTTTACCTGAAAAAACAGAAACGGACCGTGGAAGAGTACCAGTGGCA
ACATTACGATGAACAATGCAATCTCCTGAAGCAACTGTTAAGGCAGGTGTTCTTGTGCCTTGATTGTGAGGCCGGTAAAGGCTCAGAAGCCGTCGTCGCC
CAACTTCAACAGATTCAGACGGAAATCGCATCCGGTGGACCACTGAAGACGATGGATACGTCGCTCATCCCGAAAAAGCACCTCCCATGGTTGGTTAAAC
AGGATAACGTTAACCCGCAACGTTACGAATGGCTGCTCTACCGCCAGTTGACTTCGCGACTGAACGGACGCATTTATTTGCCAAATGTTACCAAATACCG
CGCACTGGAAGACGACCTGATCCCCCAGACATCGCAGGATACCTTGCTGGCCTCATCAACACTGGACAGACTAAAACAGCCCGCAGAGTTATTGTTACAG
GAGCAACAACACCGGCTGGAAAGTGCGCTCAAAGACGTTGCTCTCCATATTGATGAGGGAGACAATCGAAATGTGATCATGAAAAATCGTACCGGTACCC
GCTGGCGTCTGCCGACCAAAAGCGCTACATCTCTGGTCAACAATCCCTTTTTTAAGCGAATGCAACCGGTCGATATCGCGGATGTACTGCGGTATGTAGA
GCGCGAAACCGGGTTCATGAAATGTCTGACTCATGTACTTCCGATACAAAAACAAGGGTTCACTCATCAGGATGATTTACTGGCCATTCTGATTGCCAAC
GCCACTCACCGTGGTGTGTATGGCATGGCGCAGATCTCCGATCGAAGCTATGAACACCTGAGTACGGTGCAGGCCAACTATATCCGGCCTGAAACGCTGC
ATGATGCCAGCGATGTGATCAATAATGCGGTTGCAGCGCTACCCATCTTCCGCCACTACCATATTCAGGAGGACCAGTTGCATGCCAGTGCGGATGGTCA
GAAATTCGAAACCCATCTGGAAACCTTTAAAACCCGGTACTCCTCTAAGTATTTCGGCACCAATAAAGGGATCACGGCCATGACACTGGTAGCCAACCAC
AGCGCCCTCAATGCTCGGATCATCGGTTCCAACGAGCACGAATCACACTATATTTATGACCTGTTACAATCCAACAGCAGTGATATCAAACCTGACGTAC
TCTCGACAGATACACACGGTGTCAATCATGTTAACTTCGCCTTACTGGATCTATGCGGTTACAGTTTTGCACCGCGATACGCGCAGTTCAGTAGTGTCAT
CAACGATCTGTTTGACGTAACTGAAAATGAACATGGCGGCACCAACCTTGCCCTGAAAAAGCCCATCAGGACGAATGTCATCGAAACGGGATGGCAGGAT
ATTAGGCGCATTGTTCTGTCACTTCAGACAAAGCGAACGACACAGGCAATGCTGGTAAGAAAGTTGTCTGGTTATCCTTCGGGGCACCCGACATTACAGG
CGCTGACGGAGTATAATCGACTGGTCAAAGCGCAATATTTACTGGATTACATAGACAATGCCAGTTTGCGGCAGTACGTTCAGCGTGCTCTTAACCGGGG
AGAAGCGTGGCACTTCCTGAGACGGGCTATTGCGTCGGTAAATGGCGATCAGTTCCGAGGCAAAAACGAGTCTGAAATCGCTATCTGGAATGAATGTGCA
AGATTGCTTGCCAACGCGATCATCTACTTCAACTCCGCGATACTGAGTCATCTGCTGGAACACTTTGAAGCGAGAGGAGATGAAGAGAAAGCGGGTATCA
CTCGTTCTGTTTCGCCCGTTGCGTGGCAAAATATCAACTTAAGCGGAACGTATAACTTCACTAATACTGGGAAATTGCCCGATATTGGCGAAATAACGAG
GCCGATAGTGGATGATTAGGCTCCAAACTGAAAGTAAACCACCTCCGCAGCCATTTATTGGTGTAGTCTACAGAGGATTATGTCTTTTTAGAGGGGGAAA
TCCCCAGAACCCC
TAGGTAGATATCGTACATTTTAATACGGAACAGCCCTGATATGGCCATCAAAAACGAAATTTCTATTCTCACGAGAGCAGAACAGGCAGATCTTTATTCC
CCACCCATTTTTTCAATCGAAGAACAACGTCTGTATTTTTCTCTGAACGATGGGGAATTGGCAGTTTTTCGGTCAATTCGTCTCAGAGCTCATAGATGTT
ACTTTGTCGCGATTTTGGGATACTTTAAATCAAAGCCCGTCATCCTAGATATCGCTTACTCGCAGGTTTCTAAGGATTTAATGTTCATCAGTAAAGAGCT
GCTTGGCGGCAAGGGGCTCAGACCATTCACTCCCTCACAAAAACAAAAAGATCGACTCTACGCAAAAGTATTAGACCTTGCTGGTTATCACAAATGGGAC
GAAAGTCAGCACTTCAATTCTCTTTTCGACCACCTTGTTCAGGTGGGCAATGCCTGGCTGGAGCCGCGTTACCTCTTTGATACTGCTATTGAATTCCTAG
CCAGTCACAGCATTGCTATCCCCAGGTATACCGTACTCCAGAGACTGATAAGCAGAACAATGCAGCAGGTCAGAAAAGACCTGGCGCACCAACTTAATCA
ACTCACCAGTCCTGAACTTCACGTCTTTCTGGACAGCATAACAGCCATTGATGACGGACTAAGCCTGAACCAGCTCAGAGGCGGTGCAAAAAGTCTGACC
GTACCTGAGCTTAAAAAAGAGCTTGCCCTTTATCATCAGTTAGCGCCATGGCGCACGCAAATCAATGGCGTTATCGATGGGCTTAATCTGTCTCTTAAAA
ATCGACAACACTTCGGTGAGCTCATCAACTATTACGGCAGTAAACTCAAACGATTCAAACGCGCACAGCAGCATCTATGGTTGCTATGTCACCTGACAGA
GCGGATACAACTGGCACTGGAACGGTTAACTGATGGGTTCATTTACCATATCCGCAAGCAACAAGAAGCTGCCAACACCTTTGCACAACAAGCAGTGTTC
CTGTCCTGGCAGTCAGCCGCGGACAATGTCACAAAAGCGGCAGAGTTACTGCATCTGTTTGTGGATGAGAACATTGATGATAATCAACCCTTCTCAGTAG
TCAGACAACAGGCATTGAAGGTCATGAATGACAGGGATATCCAGACCCTCTGCCTTTACCTGAAAAAACAGAAACGGACCGTGGAAGAGTACCAGTGGCA
ACATTACGATGAACAATGCAATCTCCTGAAGCAACTGTTAAGGCAGGTGTTCTTGTGCCTTGATTGTGAGGCCGGTAAAGGCTCAGAAGCCGTCGTCGCC
CAACTTCAACAGATTCAGACGGAAATCGCATCCGGTGGACCACTGAAGACGATGGATACGTCGCTCATCCCGAAAAAGCACCTCCCATGGTTGGTTAAAC
AGGATAACGTTAACCCGCAACGTTACGAATGGCTGCTCTACCGCCAGTTGACTTCGCGACTGAACGGACGCATTTATTTGCCAAATGTTACCAAATACCG
CGCACTGGAAGACGACCTGATCCCCCAGACATCGCAGGATACCTTGCTGGCCTCATCAACACTGGACAGACTAAAACAGCCCGCAGAGTTATTGTTACAG
GAGCAACAACACCGGCTGGAAAGTGCGCTCAAAGACGTTGCTCTCCATATTGATGAGGGAGACAATCGAAATGTGATCATGAAAAATCGTACCGGTACCC
GCTGGCGTCTGCCGACCAAAAGCGCTACATCTCTGGTCAACAATCCCTTTTTTAAGCGAATGCAACCGGTCGATATCGCGGATGTACTGCGGTATGTAGA
GCGCGAAACCGGGTTCATGAAATGTCTGACTCATGTACTTCCGATACAAAAACAAGGGTTCACTCATCAGGATGATTTACTGGCCATTCTGATTGCCAAC
GCCACTCACCGTGGTGTGTATGGCATGGCGCAGATCTCCGATCGAAGCTATGAACACCTGAGTACGGTGCAGGCCAACTATATCCGGCCTGAAACGCTGC
ATGATGCCAGCGATGTGATCAATAATGCGGTTGCAGCGCTACCCATCTTCCGCCACTACCATATTCAGGAGGACCAGTTGCATGCCAGTGCGGATGGTCA
GAAATTCGAAACCCATCTGGAAACCTTTAAAACCCGGTACTCCTCTAAGTATTTCGGCACCAATAAAGGGATCACGGCCATGACACTGGTAGCCAACCAC
AGCGCCCTCAATGCTCGGATCATCGGTTCCAACGAGCACGAATCACACTATATTTATGACCTGTTACAATCCAACAGCAGTGATATCAAACCTGACGTAC
TCTCGACAGATACACACGGTGTCAATCATGTTAACTTCGCCTTACTGGATCTATGCGGTTACAGTTTTGCACCGCGATACGCGCAGTTCAGTAGTGTCAT
CAACGATCTGTTTGACGTAACTGAAAATGAACATGGCGGCACCAACCTTGCCCTGAAAAAGCCCATCAGGACGAATGTCATCGAAACGGGATGGCAGGAT
ATTAGGCGCATTGTTCTGTCACTTCAGACAAAGCGAACGACACAGGCAATGCTGGTAAGAAAGTTGTCTGGTTATCCTTCGGGGCACCCGACATTACAGG
CGCTGACGGAGTATAATCGACTGGTCAAAGCGCAATATTTACTGGATTACATAGACAATGCCAGTTTGCGGCAGTACGTTCAGCGTGCTCTTAACCGGGG
AGAAGCGTGGCACTTCCTGAGACGGGCTATTGCGTCGGTAAATGGCGATCAGTTCCGAGGCAAAAACGAGTCTGAAATCGCTATCTGGAATGAATGTGCA
AGATTGCTTGCCAACGCGATCATCTACTTCAACTCCGCGATACTGAGTCATCTGCTGGAACACTTTGAAGCGAGAGGAGATGAAGAGAAAGCGGGTATCA
CTCGTTCTGTTTCGCCCGTTGCGTGGCAAAATATCAACTTAAGCGGAACGTATAACTTCACTAATACTGGGAAATTGCCCGATATTGGCGAAATAACGAG
GCCGATAGTGGATGATTAGGCTCCAAACTGAAAGTAAACCACCTCCGCAGCCATTTATTGGTGTAGTCTACAGAGGATTATGTCTTTTTAGAGGGGGAAA
TCCCCAGAACCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 2979 bp | 992 aa | 141 | 3119 | + | No |
Chemistry : DDE
ORF sequence :
MAIKNEISILTRAEQADLYSPPIFSIEEQRLYFSLNDGELAVFRSIRLRAHRCYFVAILGYFKSKPVILDIAYSQVSKDLMFISKELLGGKGLRPFTPSQ
KQKDRLYAKVLDLAGYHKWDESQHFNSLFDHLVQVGNAWLEPRYLFDTAIEFLASHSIAIPRYTVLQRLISRTMQQVRKDLAHQLNQLTSPELHVFLDSI
TAIDDGLSLNQLRGGAKSLTVPELKKELALYHQLAPWRTQINGVIDGLNLSLKNRQHFGELINYYGSKLKRFKRAQQHLWLLCHLTERIQLALERLTDGF
IYHIRKQQEAANTFAQQAVFLSWQSAADNVTKAAELLHLFVDENIDDNQPFSVVRQQALKVMNDRDIQTLCLYLKKQKRTVEEYQWQHYDEQCNLLKQLL
RQVFLCLDCEAGKGSEAVVAQLQQIQTEIASGGPLKTMDTSLIPKKHLPWLVKQDNVNPQRYEWLLYRQLTSRLNGRIYLPNVTKYRALEDDLIPQTSQD
TLLASSTLDRLKQPAELLLQEQQHRLESALKDVALHIDEGDNRNVIMKNRTGTRWRLPTKSATSLVNNPFFKRMQPVDIADVLRYVERETGFMKCLTHVL
PIQKQGFTHQDDLLAILIANATHRGVYGMAQISDRSYEHLSTVQANYIRPETLHDASDVINNAVAALPIFRHYHIQEDQLHASADGQKFETHLETFKTRY
SSKYFGTNKGITAMTLVANHSALNARIIGSNEHESHYIYDLLQSNSSDIKPDVLSTDTHGVNHVNFALLDLCGYSFAPRYAQFSSVINDLFDVTENEHGG
TNLALKKPIRTNVIETGWQDIRRIVLSLQTKRTTQAMLVRKLSGYPSGHPTLQALTEYNRLVKAQYLLDYIDNASLRQYVQRALNRGEAWHFLRRAIASV
NGDQFRGKNESEIAIWNECARLLANAIIYFNSAILSHLLEHFEARGDEEKAGITRSVSPVAWQNINLSGTYNFTNTGKLPDIGEITRPIVDD
KQKDRLYAKVLDLAGYHKWDESQHFNSLFDHLVQVGNAWLEPRYLFDTAIEFLASHSIAIPRYTVLQRLISRTMQQVRKDLAHQLNQLTSPELHVFLDSI
TAIDDGLSLNQLRGGAKSLTVPELKKELALYHQLAPWRTQINGVIDGLNLSLKNRQHFGELINYYGSKLKRFKRAQQHLWLLCHLTERIQLALERLTDGF
IYHIRKQQEAANTFAQQAVFLSWQSAADNVTKAAELLHLFVDENIDDNQPFSVVRQQALKVMNDRDIQTLCLYLKKQKRTVEEYQWQHYDEQCNLLKQLL
RQVFLCLDCEAGKGSEAVVAQLQQIQTEIASGGPLKTMDTSLIPKKHLPWLVKQDNVNPQRYEWLLYRQLTSRLNGRIYLPNVTKYRALEDDLIPQTSQD
TLLASSTLDRLKQPAELLLQEQQHRLESALKDVALHIDEGDNRNVIMKNRTGTRWRLPTKSATSLVNNPFFKRMQPVDIADVLRYVERETGFMKCLTHVL
PIQKQGFTHQDDLLAILIANATHRGVYGMAQISDRSYEHLSTVQANYIRPETLHDASDVINNAVAALPIFRHYHIQEDQLHASADGQKFETHLETFKTRY
SSKYFGTNKGITAMTLVANHSALNARIIGSNEHESHYIYDLLQSNSSDIKPDVLSTDTHGVNHVNFALLDLCGYSFAPRYAQFSSVINDLFDVTENEHGG
TNLALKKPIRTNVIETGWQDIRRIVLSLQTKRTTQAMLVRKLSGYPSGHPTLQALTEYNRLVKAQYLLDYIDNASLRQYVQRALNRGEAWHFLRRAIASV
NGDQFRGKNESEIAIWNECARLLANAIIYFNSAILSHLLEHFEARGDEEKAGITRSVSPVAWQNINLSGTYNFTNTGKLPDIGEITRPIVDD
Blast result :
Comments
2 copies of ISShfr9 are encoded by Shewanella frigidimarina, but one is interrupted by a Tn3 family transposon. The ISShfr9 transposase has 52% identity to IS3000.
References
1] Romine, M. F. (2008) Direct submission