ISMno2
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011894 | ND | Methylobacterium nodulans | Methylobacterium nodulans ORS 2060 Methylobacterium nodulans ORS 2060 plasmid pMNOD02 Methylobacterium nodulans ORS 2060 plasmid pMNOD01 |
DNA section
IS Length : 1419 bp
Ends
IR Length : 21/27
IRL : GTAATTACCCACGGGGTGCGCGCGAGCGCGGCGTGACCCACAAGCGAGGC
IRR : GTAATTACCCACGCCCGTGGCGGTAGGATCAGGCGCGGATGATGTTGAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTCAGGGTGC | ACATATCG | CTGGACGACG | 8 |
| CGCGAACATT | GGCGTGGG | ACAAAGATGG | 8 |
| ATCGACGGCA | CCGGAGCG | GGTGCCCATC | 8 |
| TGTCGATTGC | CCGGTAGA | GATACCGCCA | 8 |
| GTTGATCATC | CTCGGAGG | AGCATACTCC | 8 |
| TGCCACTTGT | CTACGAGC | TGCCTTCGCC | 8 |
| GGAACCGACC | CCAAAGTC | GGCGTGTCGA | 8 |
| GCGGCAGAGC | GCGCGAAG | CGCAAGGCCA | 8 |
DNA sequence
GTAATTACCCACGGGGTGCGCGCGAGCGCGGCGTGACCCACAAGCGAGGCCGCTAGAGGGAGTTCGCCGCGCCTCTTGCCGGGAGGCGATTTGGCTGATT
CCCTCGTGGGATGGGCCGCAGCGAGCTGGAACGCCTGAGCAAGCAGGAGCTGATCGAGCTGGTGCTGCGGCTGCAGCGCCCCGAGAAGACCTCGCGCACC
TCGTCCAAGCCGCCCTCGACCGACCGCAAGGAGCGGCGCGAGCAGGCCAAGCCCGGCGGCGCCAAGCCGGGTCACGAGGGACATAGCCGAACGCTCAGCC
CCGATCCCGACGAGATCGTCGCTCACCGCCCGGGTCACTGCCCCTGCTGCGGAGGTGTTCTGGCTCCGGATCTGCCGGCCGAGATCGTCAGCGTGTGCGA
GCAGATCGACTTGCCCGCGGTGGCACCGATGGTCACCCAGCATCAACGGCTCGCCGTCCGCTGCCCGGCTTGTGGCACGCGCGTCGTCGCTCCAGTGCCG
CAGGCCGCACGCGGCACGCCGTTCGGCCCGCGCCTGCACGCGGTGGCGGTGTATCTGAAGACCTTCCAGGCTCTGTCCTACGAGCGGCTGCAGGCCGCGT
TGTCGGACCTGTTCGGGCTCACCTTGAGCCAGGGCGGGCTGATGAACCTGCTGCGTCGGGCCCAGAGGCAGTTCCGTGCCGATCGCGAAGCTGCGGTCTC
GGCGCTGCGCCGGGCCGCGGTAGTCGCCTCGGATGAGACCGGCGTGCGCATCGAGGGCAGCAACTCGTATCATTGGGTGTTCCGCTCGGACGCAGCGGTC
GTTCATCACGCGGCCTCGACGCGGGCCGCCGCGGTGGTGCACGCGATGATGGACGGACACCGGCCGTCCGTGTGGCTGTCGGACCGCTACACCGCCCAGC
AGGGCCACGGTGAGCATCACCAGACCTGCCTGGCGCATTTGGCCCGTGACGTCGCCTACGCGGTCGAGGTCAGCGACGACCCCGTGCCGCTGCGCCTACA
ACTCTGGCTGGGAAGCGTGTTCAGCCTGGCCGAGCGCGTCACCGACCTGGCCGCCTCGACGCTCTCGGCCAAGCGCCGGGCCCTGGATCGGCAGCTGTCC
GCCATCCTCGCTGCACCAAGCCGCTGCGATCTGACCCGCGCTCTGCAAGCCAAGATCGGCCGAGCCCGCGACCAGCTCCTGGTCTTCCTCGACCATCCCG
GCCGCGTGGCGGTCACCAACAACGCTTGCGAGCGCGCGCTGCGCCCGGCAGTGGTGCAGCGGAAGGTGACGAACGGCTATCGGGCCATGTGGGCGGCCGC
GGGTGAGGCGGACGTGCGCACCGTCGTCGACACGGCCCGCCTCTCGGGGGCCGGCACCTTCGCCACTATCCTCAACATCATCCGCGCCTGATCCTACCGC
CACGGGCGTGGGTAATTAC
CCCTCGTGGGATGGGCCGCAGCGAGCTGGAACGCCTGAGCAAGCAGGAGCTGATCGAGCTGGTGCTGCGGCTGCAGCGCCCCGAGAAGACCTCGCGCACC
TCGTCCAAGCCGCCCTCGACCGACCGCAAGGAGCGGCGCGAGCAGGCCAAGCCCGGCGGCGCCAAGCCGGGTCACGAGGGACATAGCCGAACGCTCAGCC
CCGATCCCGACGAGATCGTCGCTCACCGCCCGGGTCACTGCCCCTGCTGCGGAGGTGTTCTGGCTCCGGATCTGCCGGCCGAGATCGTCAGCGTGTGCGA
GCAGATCGACTTGCCCGCGGTGGCACCGATGGTCACCCAGCATCAACGGCTCGCCGTCCGCTGCCCGGCTTGTGGCACGCGCGTCGTCGCTCCAGTGCCG
CAGGCCGCACGCGGCACGCCGTTCGGCCCGCGCCTGCACGCGGTGGCGGTGTATCTGAAGACCTTCCAGGCTCTGTCCTACGAGCGGCTGCAGGCCGCGT
TGTCGGACCTGTTCGGGCTCACCTTGAGCCAGGGCGGGCTGATGAACCTGCTGCGTCGGGCCCAGAGGCAGTTCCGTGCCGATCGCGAAGCTGCGGTCTC
GGCGCTGCGCCGGGCCGCGGTAGTCGCCTCGGATGAGACCGGCGTGCGCATCGAGGGCAGCAACTCGTATCATTGGGTGTTCCGCTCGGACGCAGCGGTC
GTTCATCACGCGGCCTCGACGCGGGCCGCCGCGGTGGTGCACGCGATGATGGACGGACACCGGCCGTCCGTGTGGCTGTCGGACCGCTACACCGCCCAGC
AGGGCCACGGTGAGCATCACCAGACCTGCCTGGCGCATTTGGCCCGTGACGTCGCCTACGCGGTCGAGGTCAGCGACGACCCCGTGCCGCTGCGCCTACA
ACTCTGGCTGGGAAGCGTGTTCAGCCTGGCCGAGCGCGTCACCGACCTGGCCGCCTCGACGCTCTCGGCCAAGCGCCGGGCCCTGGATCGGCAGCTGTCC
GCCATCCTCGCTGCACCAAGCCGCTGCGATCTGACCCGCGCTCTGCAAGCCAAGATCGGCCGAGCCCGCGACCAGCTCCTGGTCTTCCTCGACCATCCCG
GCCGCGTGGCGGTCACCAACAACGCTTGCGAGCGCGCGCTGCGCCCGGCAGTGGTGCAGCGGAAGGTGACGAACGGCTATCGGGCCATGTGGGCGGCCGC
GGGTGAGGCGGACGTGCGCACCGTCGTCGACACGGCCCGCCTCTCGGGGGCCGGCACCTTCGCCACTATCCTCAACATCATCCGCGCCTGATCCTACCGC
CACGGGCGTGGGTAATTAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1281 bp | 426 aa | 111 | 1391 | + | No |
Chemistry : DDE
ORF sequence :
MGRSELERLSKQELIELVLRLQRPEKTSRTSSKPPSTDRKERREQAKPGGAKPGHEGHSRTLSPDPDEIVAHRPGHCPCCGGVLAPDLPAEIVSVCEQID
LPAVAPMVTQHQRLAVRCPACGTRVVAPVPQAARGTPFGPRLHAVAVYLKTFQALSYERLQAALSDLFGLTLSQGGLMNLLRRAQRQFRADREAAVSALR
RAAVVASDETGVRIEGSNSYHWVFRSDAAVVHHAASTRAAAVVHAMMDGHRPSVWLSDRYTAQQGHGEHHQTCLAHLARDVAYAVEVSDDPVPLRLQLWL
GSVFSLAERVTDLAASTLSAKRRALDRQLSAILAAPSRCDLTRALQAKIGRARDQLLVFLDHPGRVAVTNNACERALRPAVVQRKVTNGYRAMWAAAGEA
DVRTVVDTARLSGAGTFATILNIIRA
LPAVAPMVTQHQRLAVRCPACGTRVVAPVPQAARGTPFGPRLHAVAVYLKTFQALSYERLQAALSDLFGLTLSQGGLMNLLRRAQRQFRADREAAVSALR
RAAVVASDETGVRIEGSNSYHWVFRSDAAVVHHAASTRAAAVVHAMMDGHRPSVWLSDRYTAQQGHGEHHQTCLAHLARDVAYAVEVSDDPVPLRLQLWL
GSVFSLAERVTDLAASTLSAKRRALDRQLSAILAAPSRCDLTRALQAKIGRARDQLLVFLDHPGRVAVTNNACERALRPAVVQRKVTNGYRAMWAAAGEA
DVRTVVDTARLSGAGTFATILNIIRA
Blast result :
Comments
ISMno2 is 54% aa similar to ISRhru2.
References
1] ISfinder annotation (2008).
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S., Larimer,F., Land,M.L., Hauser,L., Marx,C.J. and Richardson,P. (2007) Direct Submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S., Larimer,F., Land,M.L., Hauser,L., Marx,C.J. and Richardson,P. (2007) Direct Submission GenBank.