ISAli12B
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Azospirillum lipoferum | Azospirillum lipoferum 4B plasmid p4AZO 551 Azospirillum lipoferum 4B plasmid p5AZO 552 Azospirillum lipoferum 4B plasmid p6AZO 553 Azospirillum lipoferum 4B plasmid p1AZO 548 Azospirillum lipoferum 4B plasmid p3AZO 550 |
DNA section
IS Length : 844 bp
Ends
IR Length : 16
IRL : GGGCGTGTCGTCAGTTATGGGATTCCCAGCGGTAGGAATGCGTGATTCAT
IRR : GGGCGTGTCGTCAGTTGAGCCAGATGACAGCCGACACGAGGTAAACAGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCGCCGGCCCGGTTTTCC | TA | AGGCCGGCGGCAACTGTC | 2 |
DNA sequence
GGGCGTGTCGTCAGTTATGGGATTCCCAGCGGTAGGAATGCGTGATTCATAGAGGGTCGGCTGACGGGAGGGCCGACGAATGCGACGCTATGCTTTGCGC
GACGATCAGTGGGAGCGGGTCCAGGATTTTCTGCCGGGGCGGGTAGGCCATGTCGGGGGGACGGCGAAGGACAACCGCCTGTTCGTCGAGGCGGTGCTGT
ACCGTTACCGGGCTGGCATTCCCTGGCGCGACCTGCCCACCCGGTTCGGGGATTGGAAGAACACCCACCGCCGCTACCGCCGCTGGTGCGAGAGCGGGGT
GTTCGAGCGCCTTTTCAAAGCCCTGGCCGAGGACAGCGACAACGAGTACATGATGCTCGACGCGACCATCGTCCGCGCCCACCAGCACAGCGCCGGGGCG
CGAAAAAAGGGGGCGCCTGCCAAGCCATCGGCCGATCACGAGGGGGGCTGACGACGAAGATCCATGCCATCGTGGACGCCTTGGGCAACCCGGTGGCGAT
CTCCCTGACCCCCGGTCAGGCCTCTGACTTCAGCCAAGCCGCCCCTCTGCTCGACGCGGTCGAGCCGCAGGCCCTCATCGCCGACAAGGCCTATGATGGC
GACGCCCTGATCGACGCATTGAAGGAGCGCGACATCGTCCCCGTCATCCCGTCCAAGGCTAATCGGCTGATCGCCCGCGATACCGATTTCGCCTTGTACC
GCGAACGTAATCTCGTCGAGCGATTCTTCAACAAGCTGAAGGGCTTCCGAGCCATTGCAACTCGATACGACAAGCTTGCCAGCACTTTCTTGGCGGCTGT
TTACCTCGTGTCGGCTGTCATCTGGCTCAACTGACGACACGCCC
GACGATCAGTGGGAGCGGGTCCAGGATTTTCTGCCGGGGCGGGTAGGCCATGTCGGGGGGACGGCGAAGGACAACCGCCTGTTCGTCGAGGCGGTGCTGT
ACCGTTACCGGGCTGGCATTCCCTGGCGCGACCTGCCCACCCGGTTCGGGGATTGGAAGAACACCCACCGCCGCTACCGCCGCTGGTGCGAGAGCGGGGT
GTTCGAGCGCCTTTTCAAAGCCCTGGCCGAGGACAGCGACAACGAGTACATGATGCTCGACGCGACCATCGTCCGCGCCCACCAGCACAGCGCCGGGGCG
CGAAAAAAGGGGGCGCCTGCCAAGCCATCGGCCGATCACGAGGGGGGCTGACGACGAAGATCCATGCCATCGTGGACGCCTTGGGCAACCCGGTGGCGAT
CTCCCTGACCCCCGGTCAGGCCTCTGACTTCAGCCAAGCCGCCCCTCTGCTCGACGCGGTCGAGCCGCAGGCCCTCATCGCCGACAAGGCCTATGATGGC
GACGCCCTGATCGACGCATTGAAGGAGCGCGACATCGTCCCCGTCATCCCGTCCAAGGCTAATCGGCTGATCGCCCGCGATACCGATTTCGCCTTGTACC
GCGAACGTAATCTCGTCGAGCGATTCTTCAACAAGCTGAAGGGCTTCCGAGCCATTGCAACTCGATACGACAAGCTTGCCAGCACTTTCTTGGCGGCTGT
TTACCTCGTGTCGGCTGTCATCTGGCTCAACTGACGACACGCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 372 bp | 123 aa | 80 | 451 | + | No |
Description : First part of the transposase
ORF sequence :
MRRYALRDDQWERVQDFLPGRVGHVGGTAKDNRLFVEAVLYRYRAGIPWRDLPTRFGDWKNTHRRYRRWCESGVFERLFKALAEDSDNEYMMLDATIVRA
HQHSAGARKKGAPAKPSADHEGG
HQHSAGARKKGAPAKPSADHEGG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 534 bp | 177 aa | 301 | 834 | + | No |
Description : Second part of the transposase
ORF sequence :
VRAPFQSPGRGQRQRVHDARRDHRPRPPAQRRGAKKGGACQAIGRSRGGLTTKIHAIVDALGNPVAISLTPGQASDFSQAAPLLDAVEPQALIADKAYDG
DALIDALKERDIVPVIPSKANRLIARDTDFALYRERNLVERFFNKLKGFRAIATRYDKLASTFLAAVYLVSAVIWLN
DALIDALKERDIVPVIPSKANRLIARDTDFALYRERNLVERFFNKLKGFRAIATRYDKLASTFLAAVYLVSAVIWLN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 755 bp | 251 aa | 80 | 834 | + | Yes |
Chemistry : DDE
ORF sequence :
MRRYALRDDQWERVQDFLPGRVGHVGGTAKDNRLFVEAVLYRYRAGIPWRDLPTRFGDWKNTHRRYRRWCESGVFERLFKALAEDSDNEYMMLDATIVRA
HQHSAGARKKGGACQAIGRSRGGLTTKIHAIVDALGNPVAISLTPGQASDFSQAAPLLDAVEPQALIADKAYDGDALIDALKERDIVPVIPSKANRLIAR
DTDFALYRERNLVERFFNKLKGFRAIATRYDKLASTFLAAVYLVSAVIWLN
HQHSAGARKKGGACQAIGRSRGGLTTKIHAIVDALGNPVAISLTPGQASDFSQAAPLLDAVEPQALIADKAYDGDALIDALKERDIVPVIPSKANRLIAR
DTDFALYRERNLVERFFNKLKGFRAIATRYDKLASTFLAAVYLVSAVIWLN
Blast result :
Comments
ISAli12B is 67% aa similar to ISAli9.
All of these elements contain an identical potential frameshift window. However ISAli12A contains only a single ORF not requiring frameshifting whereas the equivalent transposase of ISAli12B requires a frameshifting event.
The third ORF is putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
All of these elements contain an identical potential frameshift window. However ISAli12A contains only a single ORF not requiring frameshifting whereas the equivalent transposase of ISAli12B requires a frameshifting event.
The third ORF is putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2008)