ISSpu12
- Family IS4
- Group IS50
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008750 | ND | Shewanella putrefaciens | Shewanella putrefaciens 200 Shewanella putrefaciens W3-18-1 |
DNA section
IS Length : 1500 bp
Ends
IR Length : 14/18
IRL : GCCTGTCTCTTATACACAAATCCCCCGATAAATTCGGGGATTTTTTTGAA
IRR : GCCTATCTCTTGATCACATCTCGTGTTCAAGAGATAGAGCCAATTCGTAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAAAGGCGCCATGT | CTAGGCGCCTTTTT | 0 |
DNA sequence
GCCTGTCTCTTATACACAAATCCCCCGATAAATTCGGGGATTTTTTTGAACTCGCGCTGATGTTGTTGATCTGATCTACAAAACAACTTTAGGCGCACGC
TATGAATTTTATTTCCAACCCAGCACAATGGGCCCAACATCTCTTCCAACATGCCAGTTTAGGTGATGCTCGGCGCGTCAAACGCTTGATAGCATTATCG
GCAACGCTGGCTGCTCATATGGGAAAATCTGTGCCGCAAGCCAGTCAAACTACCGCTGATATTGAAGCCGCTTACCGTTTTATCCGCAATGAGGCCATTT
CGGCCGATGCCATCGCCGAAGCAGGCTTTATGGCAACGAAGCAAGAAGCCTTAGCATTTGACACCTTACTGGCACTGGAAGACACCAGCTCGCTAAATTT
TTCTCACAAAGGGGTCCGTGATGAACTGGGACATATTACATCTCATCTATCGAGCCGCGGATTTCAAGCACATTCAGTATTGCTGTACGCACCATCACAT
AACCAAGTGATTGGTTTAATTGAACAACATTTATGGACCCGCGACATAGCTACGATGGGAAAGCACAAAAACGCAACCAAAAGACCTTACTGGGAAAAAG
AGAGTTTTAAATGGCAGCAGGCCTCACAAAATGTCGCTAACCGTTTGGGTCAACACATGGCACATGCGGTCAGCGTCTGTGACCGCGAAGCGGATTTAAT
TGAGTACCTGCAATATAAACTCGAACATCAGCAACGGTTTGTGGTGCGTTCGATGATAAGTCGGCATATAGAAGAAGCCGAAGGTAAGTTACATCTGTAC
GGCCATTCGTTGCAAAGTGCTGGGGAGCGAATGGTAGAGGTGGTACAAAAAGGTGGCCGCAAAGCCAGAGTCGCGACCTGTGAAGTCCGTTTTGCACCGG
TAACCCTGAAAATGCCCAGCAATAAAGTTGGCGAAAGTACGCCGTTGTTTTATGTCAGTTGTATTGAAAAAGGCAATGACGATGGATTGTGCTGGCACCT
GCTGACGTCGGAGCCCGTCACCAGGACGGAACAAGCACTGACGATCTTGTGCTGGTATGAAAAGCGCTGGCTGATAGAGGACTTTCATAAATCCTGGAAA
AGCGGTGGGACTCAGGTTGAAGATTTGCGCTTACAAAGCAAAGGTAATTTGGAGCGTATGATTACGATCCTAGCGTTTATAGCGGTGCGAGTTCAGCAGT
TGCGCCATCTTGGGTTACAAGAAGAACAGGCAAAGCAACAAAGTTGTGAGACATTGTTGGGTTGCAAAGCGTGGAAATTACTGTGGCTGAAAGTTGAACA
ATGCAAACCACCCAAGCAAGCACCCAGTGTGCATTGGGCTTACCTAAGTTTAGGAAAATTAGCGGGTTGGCACGATAGTAAAGGAACGGGTGTAGTAGGT
TGGGAGCGGTTATGGGAAGGATGGTTCAAACTGCAAACCATCCTTGAAGGCTACGAATTGGCTCTATCTCTTGAACACGAGATGTGATCAAGAGATAGGC
TATGAATTTTATTTCCAACCCAGCACAATGGGCCCAACATCTCTTCCAACATGCCAGTTTAGGTGATGCTCGGCGCGTCAAACGCTTGATAGCATTATCG
GCAACGCTGGCTGCTCATATGGGAAAATCTGTGCCGCAAGCCAGTCAAACTACCGCTGATATTGAAGCCGCTTACCGTTTTATCCGCAATGAGGCCATTT
CGGCCGATGCCATCGCCGAAGCAGGCTTTATGGCAACGAAGCAAGAAGCCTTAGCATTTGACACCTTACTGGCACTGGAAGACACCAGCTCGCTAAATTT
TTCTCACAAAGGGGTCCGTGATGAACTGGGACATATTACATCTCATCTATCGAGCCGCGGATTTCAAGCACATTCAGTATTGCTGTACGCACCATCACAT
AACCAAGTGATTGGTTTAATTGAACAACATTTATGGACCCGCGACATAGCTACGATGGGAAAGCACAAAAACGCAACCAAAAGACCTTACTGGGAAAAAG
AGAGTTTTAAATGGCAGCAGGCCTCACAAAATGTCGCTAACCGTTTGGGTCAACACATGGCACATGCGGTCAGCGTCTGTGACCGCGAAGCGGATTTAAT
TGAGTACCTGCAATATAAACTCGAACATCAGCAACGGTTTGTGGTGCGTTCGATGATAAGTCGGCATATAGAAGAAGCCGAAGGTAAGTTACATCTGTAC
GGCCATTCGTTGCAAAGTGCTGGGGAGCGAATGGTAGAGGTGGTACAAAAAGGTGGCCGCAAAGCCAGAGTCGCGACCTGTGAAGTCCGTTTTGCACCGG
TAACCCTGAAAATGCCCAGCAATAAAGTTGGCGAAAGTACGCCGTTGTTTTATGTCAGTTGTATTGAAAAAGGCAATGACGATGGATTGTGCTGGCACCT
GCTGACGTCGGAGCCCGTCACCAGGACGGAACAAGCACTGACGATCTTGTGCTGGTATGAAAAGCGCTGGCTGATAGAGGACTTTCATAAATCCTGGAAA
AGCGGTGGGACTCAGGTTGAAGATTTGCGCTTACAAAGCAAAGGTAATTTGGAGCGTATGATTACGATCCTAGCGTTTATAGCGGTGCGAGTTCAGCAGT
TGCGCCATCTTGGGTTACAAGAAGAACAGGCAAAGCAACAAAGTTGTGAGACATTGTTGGGTTGCAAAGCGTGGAAATTACTGTGGCTGAAAGTTGAACA
ATGCAAACCACCCAAGCAAGCACCCAGTGTGCATTGGGCTTACCTAAGTTTAGGAAAATTAGCGGGTTGGCACGATAGTAAAGGAACGGGTGTAGTAGGT
TGGGAGCGGTTATGGGAAGGATGGTTCAAACTGCAAACCATCCTTGAAGGCTACGAATTGGCTCTATCTCTTGAACACGAGATGTGATCAAGAGATAGGC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1386 bp | 461 aa | 102 | 1487 | + | No |
Chemistry : DDE
ORF sequence :
MNFISNPAQWAQHLFQHASLGDARRVKRLIALSATLAAHMGKSVPQASQTTADIEAAYRFIRNEAISADAIAEAGFMATKQEALAFDTLLALEDTSSLNF
SHKGVRDELGHITSHLSSRGFQAHSVLLYAPSHNQVIGLIEQHLWTRDIATMGKHKNATKRPYWEKESFKWQQASQNVANRLGQHMAHAVSVCDREADLI
EYLQYKLEHQQRFVVRSMISRHIEEAEGKLHLYGHSLQSAGERMVEVVQKGGRKARVATCEVRFAPVTLKMPSNKVGESTPLFYVSCIEKGNDDGLCWHL
LTSEPVTRTEQALTILCWYEKRWLIEDFHKSWKSGGTQVEDLRLQSKGNLERMITILAFIAVRVQQLRHLGLQEEQAKQQSCETLLGCKAWKLLWLKVEQ
CKPPKQAPSVHWAYLSLGKLAGWHDSKGTGVVGWERLWEGWFKLQTILEGYELALSLEHEM
SHKGVRDELGHITSHLSSRGFQAHSVLLYAPSHNQVIGLIEQHLWTRDIATMGKHKNATKRPYWEKESFKWQQASQNVANRLGQHMAHAVSVCDREADLI
EYLQYKLEHQQRFVVRSMISRHIEEAEGKLHLYGHSLQSAGERMVEVVQKGGRKARVATCEVRFAPVTLKMPSNKVGESTPLFYVSCIEKGNDDGLCWHL
LTSEPVTRTEQALTILCWYEKRWLIEDFHKSWKSGGTQVEDLRLQSKGNLERMITILAFIAVRVQQLRHLGLQEEQAKQQSCETLLGCKAWKLLWLKVEQ
CKPPKQAPSVHWAYLSLGKLAGWHDSKGTGVVGWERLWEGWFKLQTILEGYELALSLEHEM
Blast result :
Comments
A single copy of ISSPu12 is encoded in the S. putrefaciens W3-18-1 genome. The ISSPu12 transposase has 59% identity to ISPlu9. The site of insertion has palindromic symmetry.
References
1] Romine, M. (2008) Direct submission.