IS153

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s) ISLsa5
Accession numberTranspositionOriginHost
AJ239042 ND Lactobacillus sanfranciscensis
Lactobacillus sanfranciscensis
DNA section
IS Length : 1127 bp

Ends


IR Length : 27/37

IRL : TGGATGTAAGCTGATTCCTGAGACAACTTTTAAGAGAGGGTATAATGAAT
IRR : TGGATTGAAGTCAATATTTGAGACAGATTTTAAGAGACATTCAGAACCGC

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TGGATGTAAGCTGATTCCTGAGACAACTTTTAAGAGAGGGTATAATGAATAAATCGTCTCTCAAAAGGAAGGAATTTTTACATGCCAACTCGTTACGACA
AAGAATTCAAACAAAACATTATCAACCTATATAAGCAAGGCGAATCAGCTGCCCAACTGGCCAGAGAATATGGCATTGGCTATTCAACAGTTCATAAGTG
GATCCAGGGCCAGGCCAAAACTCAATCCGGTAAATCGCCAGACGAAATCAAAGCGATGGAAAAGCGACTGGCTTCGCTGTCTGAGGAGAACGAAATCCTA
AAAAAAGCCCTGGGCTTCCTTGCGCAGAAGTAACCAATATCTTTGATTACATTCACCAAGAAAGCCATCACCACCAGGTAACCAAGATGTGCCGAATCCT
CGGTGTTTCCAGAGCTCAGTATTATCGTTATCGATCCCCCAAACCTTCAAAACGCCGGGCCGAAGATGCGGACTTGAAACAACGGATTCTGCGGATCTTT
GCGGAATTTAAGCAACGATACGGTGTTATGAAGATCCACCATGAATTGAATCTGGAACTTCAACCACTGCAGCTTCGGTGCAGTCCACGACGGATTATCG
GCTATTCGTTCTCAAAAAAGATGAATACTGATTTAGTCTTAAAGACCCTTGAAAACGCGGTTAAAAATCGAACCATTACTGGGGACCTGATTATCCATAC
GGATTTAGGATCACAGTATACCAGCGATGATTACAACCAACGTTTAACTGAACTACATATCCGCCACTCATACAGCCGTAAGGGTTGTCCATATGATAAT
GCGCCAATGGAATCCTTTCACGCTTCCCTCAAAAAGGAATGTGTTTACCCAGTGCCGGTCTTTGAAGATTATGAAACTGCCGCTGCCGTCCTTTTTGAAT
ATGTGCATGCTATTCTACAATAGGAAGAGAATTCATAGTTCACTGGGCTACCAGACCCCCTTACAAGTTGAAATTGCAACACTTACGAGCCAAATGGCCA
CCTGATTTAATGCTTTCCAGGGTTCAAATAAGTTATTAATCGCGATTAATGATTTATTTGAGCTGTGGAAGGTAAACGCGGTTCTGAATGTCTCTTAAAA
TCTGTCTCAAATATTGACTTCAATCCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
252 bp83 aa82333+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPTRYDKEFKQNIINLYKQGESAAQLAREYGIGYSTVHKWIQGQAKTQSGKSPDEIKAMEKRLASLSEENEILKKALGFLAQK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
639 bp212 aa285923+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GERNPKKSPGLPCAEVTNIFDYIHQESHHHQVTKMCRILGVSRAQYYRYRSPKPSKRRAEDADLKQRILRIFAEFKQRYGVMKIHHELNLELQPLQLRCS
PRRIIGYSFSKKMNTDLVLKTLENAVKNRTITGDLIIHTDLGSQYTSDDYNQRLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPVFEDYETAA
AVLFEYVHAILQ

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
842 bp280 aa82923+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPTRYDKEFKQNIINLYKQGESAAQLAREYGIGYSTVHKWIQGQAKTQSGKSPDEIKAMEKRLASLSEENEILKKSPGLPCAEVTNIFDYIHQESHHHQV
TKMCRILGVSRAQYYRYRSPKPSKRRAEDADLKQRILRIFAEFKQRYGVMKIHHELNLELQPLQLRCSPRRIIGYSFSKKMNTDLVLKTLENAVKNRTIT
GDLIIHTDLGSQYTSDDYNQRLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPVFEDYETAAAVLFEYVHAILQ

 

Blast result :
Comments
IS153 is 62% (ORF1) and 75% (ORF2) aa similar to IS981.
Note : there is several differencies between the NCBI sequence and the sequence of the paper ...
There is a potential frameshift window 5'- AAAAAAG-3'.
ISLsa5 is an isoform of IS153. Accession number : EU402605. The IS is mutated and carries an indel.
References
1] Ehrmann,M.A. and Vogel,R.F. (2001) System Appl. Microbiol. 24, 443-450.
2] Chaillou,S., Daty,M., Baraige,F., Dudez,A.M., Anglade,P., Jones,R., Alpert,C.A., Champomier-Verges,M.C. and Zagorec,M. (2009) Appl. Environ. Microbiol. 75 (4), 970-980.