ISSpu10
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008750 | ND | Shewanella putrefaciens | Shewanella putrefaciens W3-18-1 |
DNA section
IS Length : 879 bp
Ends
IR Length : 15/16
IRL : GGGCTTGTTGATCTTTCGTGATGGATTATTAGACAGCATGATGGGCTGGT
IRR : GGGCCTGTTGATCTTTGCTGTTCATAATTCAGCCAACCCACATTGGCAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCAACGATT | TA | GTAATAAATT | 2 |
| CTTTTTCCAC | TA | GTTTAGTCAA | 2 |
DNA sequence
GGGCTTGTTGATCTTTCGTGATGGATTATTAGACAGCATGATGGGCTGGTATAATTACTTTCGCCAAAAACTAACCATGACAACCCATCATGCCAAGAAC
AATGTTAACAGACGAGTACTGGTCAAAGCTAATCAGATTGATGCTTAAAACGGGCCGCGTATATGACAAGCCGGAACACAGAAATACTTTCGAAGGCATT
TTGTATCGGATGCGGGTCGGTTGCCCTTGGCGCGATGTGCCAGAAGCTTTTGGAGATTGGAGCGCCATCTACCGGCGTTTTAACCTGTGGTCTAAAAAAG
GCATTCTAATGCAGCTATTCGCAGAGTTAAGGCAATTGGCTGATTTAGAGTGGGAATTTATAGACGGCAGCATCGTCAAAGCGCATCAGCATGCGACAGG
TGCTCGCTCAGAAGATAAAGAAGCGATTGGGAAAAGTCGTGGTGGTAACACGACGAAAATCCATATGGCAGTGGATAGTTGCGGCTTGCCAATAGATTTT
ATTGTGACAGGCGGTGAGGTTCATGACAGTAAAGCAGCCATAGAATTACTAAAACAACTGCCTGATGCCGAACACGTTATCGCTGACCGGGGCTATGATA
GCGAGAAGATACGAGAGCAGATCCGAGAAAAAAAGGGCGTCCCTAACATACCGCGCAAGCGTAATTCGAAGATTGGTAATGGCGATATTGACTGGTGTCT
GTACAAATATCGCCATCTGGTTGAGAACGTGTTTGCCAGGCTAAAACATTACCGCTCAGTAGCAACAAGGTATGATAAATTAGCCCGGAATTATGCAAGT
ACACTGGCCTTGGCTTGCTGCCTTATATGGCTGCCAATGTGGGTTGGCTGAATTATGAACAGCAAAGATCAACAGGCCC
AATGTTAACAGACGAGTACTGGTCAAAGCTAATCAGATTGATGCTTAAAACGGGCCGCGTATATGACAAGCCGGAACACAGAAATACTTTCGAAGGCATT
TTGTATCGGATGCGGGTCGGTTGCCCTTGGCGCGATGTGCCAGAAGCTTTTGGAGATTGGAGCGCCATCTACCGGCGTTTTAACCTGTGGTCTAAAAAAG
GCATTCTAATGCAGCTATTCGCAGAGTTAAGGCAATTGGCTGATTTAGAGTGGGAATTTATAGACGGCAGCATCGTCAAAGCGCATCAGCATGCGACAGG
TGCTCGCTCAGAAGATAAAGAAGCGATTGGGAAAAGTCGTGGTGGTAACACGACGAAAATCCATATGGCAGTGGATAGTTGCGGCTTGCCAATAGATTTT
ATTGTGACAGGCGGTGAGGTTCATGACAGTAAAGCAGCCATAGAATTACTAAAACAACTGCCTGATGCCGAACACGTTATCGCTGACCGGGGCTATGATA
GCGAGAAGATACGAGAGCAGATCCGAGAAAAAAAGGGCGTCCCTAACATACCGCGCAAGCGTAATTCGAAGATTGGTAATGGCGATATTGACTGGTGTCT
GTACAAATATCGCCATCTGGTTGAGAACGTGTTTGCCAGGCTAAAACATTACCGCTCAGTAGCAACAAGGTATGATAAATTAGCCCGGAATTATGCAAGT
ACACTGGCCTTGGCTTGCTGCCTTATATGGCTGCCAATGTGGGTTGGCTGAATTATGAACAGCAAAGATCAACAGGCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 762 bp | 253 aa | 90 | 851 | + | No |
Chemistry : DDE
ORF sequence :
MPRTMLTDEYWSKLIRLMLKTGRVYDKPEHRNTFEGILYRMRVGCPWRDVPEAFGDWSAIYRRFNLWSKKGILMQLFAELRQLADLEWEFIDGSIVKAHQ
HATGARSEDKEAIGKSRGGNTTKIHMAVDSCGLPIDFIVTGGEVHDSKAAIELLKQLPDAEHVIADRGYDSEKIREQIREKKGVPNIPRKRNSKIGNGDI
DWCLYKYRHLVENVFARLKHYRSVATRYDKLARNYASTLALACCLIWLPMWVG
HATGARSEDKEAIGKSRGGNTTKIHMAVDSCGLPIDFIVTGGEVHDSKAAIELLKQLPDAEHVIADRGYDSEKIREQIREKKGVPNIPRKRNSKIGNGDI
DWCLYKYRHLVENVFARLKHYRSVATRYDKLARNYASTLALACCLIWLPMWVG
Blast result :
Comments
Two copies of ISSPu10 are encoded in the Shewanella putrefaciens W3-18-1 genome. A duplication of the central TA of the target sequence TTAG or CTAG occured at each site. The ISSpu10 transposase has 66% identity with ISAac2.
References
1] Romine, M. (2008) Direct submission.