ISSpu9
- Family IS4
- Group IS50
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008750 | ND | Shewanella putrefaciens | Shewanella putrefaciens W3-18-1 |
DNA section
IS Length : 1490 bp
Ends
IR Length : 16/21
IRL : CTGTCTTTTATACAGAAGTCCCTCATATGATATGAGGGATTTTTTTTGAA
IRR : CTGTCTCTTGATCACAAGTCCTGTTCAAGAGACAGAGCAAGTTCGTAACC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACTAAAGCCA | GCCGCAAGGC | TGGTTTTTTA | 10 |
| GCAAAAACCG | GCGGATGAGC | TGGTTTTCTG | 10 |
| TAAAAAACCA | CCTAATGAAGG | TGGTTTTTTA | 11 |
| GCTAAAACCA | CCGACTTAGCTGC | AACCATATAG | 13 |
DNA sequence
CTGTCTTTTATACAGAAGTCCCTCATATGATATGAGGGATTTTTTTTGAACTAAATTCTTGGCTCACGATCAGATCTTTAACGGCTTTAATTGAGGCTCC
CTGAGATGATAAAAGGCAATAATGATTGGGCTGAAGAACAATTTGGTCATGCTAAACTTGGTGACCCAAGAAGAACGGCTAGGCTCGTAAAAATGGCGTC
AGACTTAGCTCAGCATCCCGGTAAGTCGGTAGTAAAATCGTCACCTTCACCAGCAAGTATGGAGGGCGCTTACCGGTTTATTCGAAACGATAATGTATCA
GCAGACGATATTGCTGAAGCCGGTTTTCGTGCGACAGTCAATCAAGCCCACCGTTACCCCCTCCTTCTCGCTATTGAAGATACAACCACCTTAAGCTACA
AGCATCGCTCTATTCGAGCGGACTTAGGACATGTCAACCAAGGTAATCGTTATCGAGGTCTGCTTGCCCATAGTATTTTGTTGTTTGCTCCTGAAACTCT
TGACGTTGTTGGATTGATTGAACAACACAGATGGACCAGAGACATCAAGACTCGAGGTATCCGCCGTGAGAATTTAAAACGACCTTACGAAGAAAAAGAA
GGTTATAAATGGGAAAGTGCATCACGTAATATGGCTGCACGTCTAGGAACCGCGATGGCTAACGTGATATCGGTTTGTGATCGTGAAGCCGACATCTACG
ATTATCTCCTTTACAAGATAGCGAACCAACAACGTTTTGTTGTGCGGTCAATGATGAGTCGTCATATAGAAGAAGGTTCAGATAAGCTTTATCATTTTGC
ATCAGAGCTTAACAGTGTGAAACAACGCCAAATCCAGATAGCTCAAAGGGGCGGTCGTAAAGCTCGAGAAGTCACGCTGGATGTTAAATATGCGGCAGTA
ACGTTAAAAACCCCGGCTAACAAAAAAGGCTCCCCCATTTCTCTGAACTATGTGGGTTGCTCCGAAGTCGGTGATGAAGAGAAGACGCTCAATTGGCATA
TTCTGACCAATGAGCCAGTAAACAGTGCCGAAGATGCTTTAAAAATTATCGGTTATTACGAGAAGCGCTGGCTGATTGAGGAGTATCATAAGGTCTGGAA
AAGTGAAGGGACCGGAGTTGAAGACCTTCGAGTACAGAGCAAAGATAACTTAGATAGATTAGCGACTATTTATGCCTTTTTAGCGGTAAGGATCTTCCAA
TTGAAATTTGCCAATGAGCAAATCAAAGACATTAGCAGTGAGAAAATCTTGTCACCAAGAGCGTGGAAGTTACTTTGGCTTAAAAGAATAAAGACGCCAC
CTCCAGAGGAAGTACCCACTGCAAAATGGGCATATGAGCACCTGGCAAGGCTTGGTGGTTGGAAAGACAGTAAAAGAAATGGAAGAGCATCAGTAAAGAC
GCTCTGGGAAGGATGGCTCAAATTGCAAGCAATCCTTGAGGGTTACGAACTTGCTCTGTCTCTTGAACAGGACTTGTGATCAAGAGACAG
CTGAGATGATAAAAGGCAATAATGATTGGGCTGAAGAACAATTTGGTCATGCTAAACTTGGTGACCCAAGAAGAACGGCTAGGCTCGTAAAAATGGCGTC
AGACTTAGCTCAGCATCCCGGTAAGTCGGTAGTAAAATCGTCACCTTCACCAGCAAGTATGGAGGGCGCTTACCGGTTTATTCGAAACGATAATGTATCA
GCAGACGATATTGCTGAAGCCGGTTTTCGTGCGACAGTCAATCAAGCCCACCGTTACCCCCTCCTTCTCGCTATTGAAGATACAACCACCTTAAGCTACA
AGCATCGCTCTATTCGAGCGGACTTAGGACATGTCAACCAAGGTAATCGTTATCGAGGTCTGCTTGCCCATAGTATTTTGTTGTTTGCTCCTGAAACTCT
TGACGTTGTTGGATTGATTGAACAACACAGATGGACCAGAGACATCAAGACTCGAGGTATCCGCCGTGAGAATTTAAAACGACCTTACGAAGAAAAAGAA
GGTTATAAATGGGAAAGTGCATCACGTAATATGGCTGCACGTCTAGGAACCGCGATGGCTAACGTGATATCGGTTTGTGATCGTGAAGCCGACATCTACG
ATTATCTCCTTTACAAGATAGCGAACCAACAACGTTTTGTTGTGCGGTCAATGATGAGTCGTCATATAGAAGAAGGTTCAGATAAGCTTTATCATTTTGC
ATCAGAGCTTAACAGTGTGAAACAACGCCAAATCCAGATAGCTCAAAGGGGCGGTCGTAAAGCTCGAGAAGTCACGCTGGATGTTAAATATGCGGCAGTA
ACGTTAAAAACCCCGGCTAACAAAAAAGGCTCCCCCATTTCTCTGAACTATGTGGGTTGCTCCGAAGTCGGTGATGAAGAGAAGACGCTCAATTGGCATA
TTCTGACCAATGAGCCAGTAAACAGTGCCGAAGATGCTTTAAAAATTATCGGTTATTACGAGAAGCGCTGGCTGATTGAGGAGTATCATAAGGTCTGGAA
AAGTGAAGGGACCGGAGTTGAAGACCTTCGAGTACAGAGCAAAGATAACTTAGATAGATTAGCGACTATTTATGCCTTTTTAGCGGTAAGGATCTTCCAA
TTGAAATTTGCCAATGAGCAAATCAAAGACATTAGCAGTGAGAAAATCTTGTCACCAAGAGCGTGGAAGTTACTTTGGCTTAAAAGAATAAAGACGCCAC
CTCCAGAGGAAGTACCCACTGCAAAATGGGCATATGAGCACCTGGCAAGGCTTGGTGGTTGGAAAGACAGTAAAAGAAATGGAAGAGCATCAGTAAAGAC
GCTCTGGGAAGGATGGCTCAAATTGCAAGCAATCCTTGAGGGTTACGAACTTGCTCTGTCTCTTGAACAGGACTTGTGATCAAGAGACAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1374 bp | 457 aa | 106 | 1479 | + | No |
Chemistry : DDE
ORF sequence :
MIKGNNDWAEEQFGHAKLGDPRRTARLVKMASDLAQHPGKSVVKSSPSPASMEGAYRFIRNDNVSADDIAEAGFRATVNQAHRYPLLLAIEDTTTLSYKH
RSIRADLGHVNQGNRYRGLLAHSILLFAPETLDVVGLIEQHRWTRDIKTRGIRRENLKRPYEEKEGYKWESASRNMAARLGTAMANVISVCDREADIYDY
LLYKIANQQRFVVRSMMSRHIEEGSDKLYHFASELNSVKQRQIQIAQRGGRKAREVTLDVKYAAVTLKTPANKKGSPISLNYVGCSEVGDEEKTLNWHIL
TNEPVNSAEDALKIIGYYEKRWLIEEYHKVWKSEGTGVEDLRVQSKDNLDRLATIYAFLAVRIFQLKFANEQIKDISSEKILSPRAWKLLWLKRIKTPPP
EEVPTAKWAYEHLARLGGWKDSKRNGRASVKTLWEGWLKLQAILEGYELALSLEQDL
RSIRADLGHVNQGNRYRGLLAHSILLFAPETLDVVGLIEQHRWTRDIKTRGIRRENLKRPYEEKEGYKWESASRNMAARLGTAMANVISVCDREADIYDY
LLYKIANQQRFVVRSMMSRHIEEGSDKLYHFASELNSVKQRQIQIAQRGGRKAREVTLDVKYAAVTLKTPANKKGSPISLNYVGCSEVGDEEKTLNWHIL
TNEPVNSAEDALKIIGYYEKRWLIEEYHKVWKSEGTGVEDLRVQSKDNLDRLATIYAFLAVRIFQLKFANEQIKDISSEKILSPRAWKLLWLKRIKTPPP
EEVPTAKWAYEHLARLGGWKDSKRNGRASVKTLWEGWLKLQAILEGYELALSLEQDL
Blast result :
Comments
ISSpu9 has 62% identity with ISUnCu9. There are 4 complete copies of this element in the Shewanella putrefaciens W3-18-1 genome. The insertion site is conserved and is a palindrome.
References
1] Romine, M. (2008) Direct submission.