ISBlma9
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AANZ01000004 | ND | Blastopirellula marina | Blastopirellula marina DSM 3645 |
DNA section
IS Length : 2606 bp
Ends
IR Length : 18/21
IRL : GTAACCGTCCGCCCAACCCCATCTTCCCACCCATGCTACCCTGGCGTCAT
IRR : GTAACCGTCCGGCCAACTACACCCCCTCGCGTTCCGCGCCGGATTCCATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATAATTCCA | GATGGAG | TGATCGACGG | 7 |
DNA sequence
GTAACCGTCCGCCCAACCCCATCTTCCCACCCATGCTACCCTGGCGTCATCACAGGCGTTTCTGGGGGCGAGGGAGAATTGATATGGCGAGCAGCAAGGG
GCCGCGGCGGGATGTTGCGAAGGAGCGGCGGTGGCGGGAGTTGTTTTCTGGCCACGCGAAGAGCGGGCTGTCGGTGCGGGCGTTTTGTTTGCAGCGGGGC
GTTTCCGAGGCTTCTTTCTATGCTTGGAAGCGGGAGTTGGCTCGGCGGGATGATGAAGCGGGCGGTGCGTCGCCGCGGTTTGTGGAAGTGATGGTTGGGG
ATGGCGCGGCGTCGGCGGAGAAGCTCTCAATGAGCGCGCCGCTGCAAATTCATCTCGCCGGCGTGCGCATCGAAGTGCCGGCCGGCTTTGATGCGGAGAC
GCTCAAGGCGGCGATCGCAGTGTTGCGAGCCGTCGCATGTTGAGCGTCCCCGGCTCGCTGAAAATTTTTATCGCCGTCGAGGCGGTCGACTTTCGTAAGA
GTCATGATGGCCTGGCCGCGATCGTCGAGCAGGCGATCGGCGAAGATCTGTTCGGCGGCGGCCTGTTCGTCTTCACCAACAAGCGGCGCGATCGGCTGAA
AATTTTGTATTGGGATAGCGACGGCTTGGCGCTCTGGTATAAACGTCTGGAGGCAGGGACGTTTCGCCTGCCGCGGGCGAACAATGACGCGACGCGACTT
GAAATCTCGGCGGCCGATTTGAATTTGATTCTCTCGGGCATTGAGCTTTCGAGCGTCAAACGCCGCAAGCGTTATCGTCGCCCGGAACCGGCCAAAAATC
CTTCTGAACAAACGCCCGTTGCGTGAGTCTTTTTTCTCTCGTGCGGCGTTTCTAAAAACATGAGCACGGACGCATTATCTCAGCTTTCGCGCGAAGAGTT
GATCGCGCTGGCGGAACGCGAACGCGAAACGTTCGCCTTGCAAGCGGAATCGCAAGCCGAAACGATTCGGCGACTTGAGCAGCAGCAAGAAACGCTCGCT
ACAAAGCTTGAATTACAAACGCAGACGATTCAAGAACTGCAGCGAAAGCGCGAGCAGTTGGAGCATCGCCTGGCGTTAGCTTTAAAGCAACTCTACGGCC
AACGCCGCGAGCGATTCGTCAGTCCCGATCAGTTGATGCTGTTCAGCGTCGAAGATATTGAACAACTCGCGCAAGAGGCGGAAGCGGAGCTGCGCGCGCA
GCAGCAAGAAAAACTTCTTGCGCGCCGCCGCGGCAAAAAGCAAGTCGGGCATGGGCGTCGCCCGTTGCCGGAGCATCTGCCGCGGGAGGTGATTCGCCAC
GAGCTTTCGGCGGAGGAAAGAAGTTGTCCGTGCTGCGGCGAAGCGCGCGCGGAGATCGGCTGCGAGTCGAGCGAGCAGCTGGAGTTTATTCCGGCGTCGT
TCAAAGTTCTGGTTCATGAGCGGGTGAAATACGCCTGTCGGGCGTGCCAGGAAAACGTTGCGATCGCGCCGCCAGCAAGCAAGCCGATCGAAAAAGGACT
GCCGGGCCCGGGCCTCTTGGCGCAAACGGTTCTCTCCAAATATGGCGATCACTTGCCGCTGTATCGGCAAGAAGATATCGTCGCGCGAAGCGGAATTGTG
CTGCGGCGCAGCACGCTCTGCGATTGGATCGCGGCGGCGGCCGTCCTCTTGACGCCGCTCTATCGGCGGATGTGCGAGCTCGTTTTAGCTTCGCGCGTGC
TGTATACCGACGACACGACGGTGAGCCTGCTCGATCCGCTGGCCTGCAAAGCGAAGCAGGCGCGCTTCTGGGCGTACCTTGGCGACGACCAGCACCCGCA
CGTTGTTTATGATTTTACCGAGAGTCGAAAACGGGATGGCCCGGCGAAATTTTTGACAGGCTTCGCAGGTTACTTGCACGCCGACGCGTACGGCGGCTAC
GACGGAATTTACGCGGGCGGCGGCGCGATCGAAGTCGCCTGTTGGGCGCACGCGCGCAGAAAATGGTTCGAGGCGCGAAAGACCGATCCCGCGCGAGCGC
ACCATGCTCTGGCGCTGATCCAAAAGTTGTATCACATCGAGCGCGACGCCAGACGCGCAAGCGACGAAGAGCGTCATGCCGCGCGACAAGAACATTCGCT
GCCGATCCTGGCGGAATTCAAAACCTGGCTCGACGCCGAGCGCGAACAGCTGCTGCCGAAAAGCCCAATCGGCCAGGCCGCGACCTACACGTTCAACCAA
TGGACGGCGCTGACGCGCTACTGCGAATCGGGCGAACTAAAAATCGACAACAACGCCGCCGAACGAACGATGCGCCCGGTGGCGATCGGCCGAAAAAACT
GGCTCTTCGTCGCCAGCCGAACGGGAGGCGAGCGCGCTGCAGTCTTAATGAGCGTCGTGCAAACTTGCAAACGGAATCAGGTCGAACCGTGGGCCTACCT
CCGCGACGTCTTCGAGCAACTGCCAAGCCTGGGCGAAAATCCAACCCGCGAAAACCTCGACCAACTCCTGCCCAACCAATGGCTGAAAGCCAACCCGAAC
CATGTCTGGCGGATCCAACAACTGCGCGAGCAAGACGATGCGTAGTTGAAGCGTTGAATGGAATCCGGCGCGGAACGCGAGGGGGTGTAGTTGGCCGGAC
GGTTAC
GCCGCGGCGGGATGTTGCGAAGGAGCGGCGGTGGCGGGAGTTGTTTTCTGGCCACGCGAAGAGCGGGCTGTCGGTGCGGGCGTTTTGTTTGCAGCGGGGC
GTTTCCGAGGCTTCTTTCTATGCTTGGAAGCGGGAGTTGGCTCGGCGGGATGATGAAGCGGGCGGTGCGTCGCCGCGGTTTGTGGAAGTGATGGTTGGGG
ATGGCGCGGCGTCGGCGGAGAAGCTCTCAATGAGCGCGCCGCTGCAAATTCATCTCGCCGGCGTGCGCATCGAAGTGCCGGCCGGCTTTGATGCGGAGAC
GCTCAAGGCGGCGATCGCAGTGTTGCGAGCCGTCGCATGTTGAGCGTCCCCGGCTCGCTGAAAATTTTTATCGCCGTCGAGGCGGTCGACTTTCGTAAGA
GTCATGATGGCCTGGCCGCGATCGTCGAGCAGGCGATCGGCGAAGATCTGTTCGGCGGCGGCCTGTTCGTCTTCACCAACAAGCGGCGCGATCGGCTGAA
AATTTTGTATTGGGATAGCGACGGCTTGGCGCTCTGGTATAAACGTCTGGAGGCAGGGACGTTTCGCCTGCCGCGGGCGAACAATGACGCGACGCGACTT
GAAATCTCGGCGGCCGATTTGAATTTGATTCTCTCGGGCATTGAGCTTTCGAGCGTCAAACGCCGCAAGCGTTATCGTCGCCCGGAACCGGCCAAAAATC
CTTCTGAACAAACGCCCGTTGCGTGAGTCTTTTTTCTCTCGTGCGGCGTTTCTAAAAACATGAGCACGGACGCATTATCTCAGCTTTCGCGCGAAGAGTT
GATCGCGCTGGCGGAACGCGAACGCGAAACGTTCGCCTTGCAAGCGGAATCGCAAGCCGAAACGATTCGGCGACTTGAGCAGCAGCAAGAAACGCTCGCT
ACAAAGCTTGAATTACAAACGCAGACGATTCAAGAACTGCAGCGAAAGCGCGAGCAGTTGGAGCATCGCCTGGCGTTAGCTTTAAAGCAACTCTACGGCC
AACGCCGCGAGCGATTCGTCAGTCCCGATCAGTTGATGCTGTTCAGCGTCGAAGATATTGAACAACTCGCGCAAGAGGCGGAAGCGGAGCTGCGCGCGCA
GCAGCAAGAAAAACTTCTTGCGCGCCGCCGCGGCAAAAAGCAAGTCGGGCATGGGCGTCGCCCGTTGCCGGAGCATCTGCCGCGGGAGGTGATTCGCCAC
GAGCTTTCGGCGGAGGAAAGAAGTTGTCCGTGCTGCGGCGAAGCGCGCGCGGAGATCGGCTGCGAGTCGAGCGAGCAGCTGGAGTTTATTCCGGCGTCGT
TCAAAGTTCTGGTTCATGAGCGGGTGAAATACGCCTGTCGGGCGTGCCAGGAAAACGTTGCGATCGCGCCGCCAGCAAGCAAGCCGATCGAAAAAGGACT
GCCGGGCCCGGGCCTCTTGGCGCAAACGGTTCTCTCCAAATATGGCGATCACTTGCCGCTGTATCGGCAAGAAGATATCGTCGCGCGAAGCGGAATTGTG
CTGCGGCGCAGCACGCTCTGCGATTGGATCGCGGCGGCGGCCGTCCTCTTGACGCCGCTCTATCGGCGGATGTGCGAGCTCGTTTTAGCTTCGCGCGTGC
TGTATACCGACGACACGACGGTGAGCCTGCTCGATCCGCTGGCCTGCAAAGCGAAGCAGGCGCGCTTCTGGGCGTACCTTGGCGACGACCAGCACCCGCA
CGTTGTTTATGATTTTACCGAGAGTCGAAAACGGGATGGCCCGGCGAAATTTTTGACAGGCTTCGCAGGTTACTTGCACGCCGACGCGTACGGCGGCTAC
GACGGAATTTACGCGGGCGGCGGCGCGATCGAAGTCGCCTGTTGGGCGCACGCGCGCAGAAAATGGTTCGAGGCGCGAAAGACCGATCCCGCGCGAGCGC
ACCATGCTCTGGCGCTGATCCAAAAGTTGTATCACATCGAGCGCGACGCCAGACGCGCAAGCGACGAAGAGCGTCATGCCGCGCGACAAGAACATTCGCT
GCCGATCCTGGCGGAATTCAAAACCTGGCTCGACGCCGAGCGCGAACAGCTGCTGCCGAAAAGCCCAATCGGCCAGGCCGCGACCTACACGTTCAACCAA
TGGACGGCGCTGACGCGCTACTGCGAATCGGGCGAACTAAAAATCGACAACAACGCCGCCGAACGAACGATGCGCCCGGTGGCGATCGGCCGAAAAAACT
GGCTCTTCGTCGCCAGCCGAACGGGAGGCGAGCGCGCTGCAGTCTTAATGAGCGTCGTGCAAACTTGCAAACGGAATCAGGTCGAACCGTGGGCCTACCT
CCGCGACGTCTTCGAGCAACTGCCAAGCCTGGGCGAAAATCCAACCCGCGAAAACCTCGACCAACTCCTGCCCAACCAATGGCTGAAAGCCAACCCGAAC
CATGTCTGGCGGATCCAACAACTGCGCGAGCAAGACGATGCGTAGTTGAAGCGTTGAATGGAATCCGGCGCGGAACGCGAGGGGGTGTAGTTGGCCGGAC
GGTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 330 bp | 109 aa | 114 | 443 | + | No |
AG : IS66 TnpA
ORF sequence :
VAKERRWRELFSGHAKSGLSVRAFCLQRGVSEASFYAWKRELARRDDEAGGASPRFVEVMVGDGAASAEKLSMSAPLQIHLAGVRIEVPAGFDAETLKAA
IAVLRAVAC
IAVLRAVAC
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 390 bp | 129 aa | 437 | 826 | + | No |
AG : IS66 TnpB
ORF sequence :
MLSVPGSLKIFIAVEAVDFRKSHDGLAAIVEQAIGEDLFGGGLFVFTNKRRDRLKILYWDSDGLALWYKRLEAGTFRLPRANNDATRLEISAADLNLILS
GIELSSVKRRKRYRRPEPAKNPSEQTPVA
GIELSSVKRRKRYRRPEPAKNPSEQTPVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1686 bp | 561 aa | 860 | 2545 | + | No |
Chemistry : DDE
ORF sequence :
MSTDALSQLSREELIALAERERETFALQAESQAETIRRLEQQQETLATKLELQTQTIQELQRKREQLEHRLALALKQLYGQRRERFVSPDQLMLFSVEDI
EQLAQEAEAELRAQQQEKLLARRRGKKQVGHGRRPLPEHLPREVIRHELSAEERSCPCCGEARAEIGCESSEQLEFIPASFKVLVHERVKYACRACQENV
AIAPPASKPIEKGLPGPGLLAQTVLSKYGDHLPLYRQEDIVARSGIVLRRSTLCDWIAAAAVLLTPLYRRMCELVLASRVLYTDDTTVSLLDPLACKAKQ
ARFWAYLGDDQHPHVVYDFTESRKRDGPAKFLTGFAGYLHADAYGGYDGIYAGGGAIEVACWAHARRKWFEARKTDPARAHHALALIQKLYHIERDARRA
SDEERHAARQEHSLPILAEFKTWLDAEREQLLPKSPIGQAATYTFNQWTALTRYCESGELKIDNNAAERTMRPVAIGRKNWLFVASRTGGERAAVLMSVV
QTCKRNQVEPWAYLRDVFEQLPSLGENPTRENLDQLLPNQWLKANPNHVWRIQQLREQDDA
EQLAQEAEAELRAQQQEKLLARRRGKKQVGHGRRPLPEHLPREVIRHELSAEERSCPCCGEARAEIGCESSEQLEFIPASFKVLVHERVKYACRACQENV
AIAPPASKPIEKGLPGPGLLAQTVLSKYGDHLPLYRQEDIVARSGIVLRRSTLCDWIAAAAVLLTPLYRRMCELVLASRVLYTDDTTVSLLDPLACKAKQ
ARFWAYLGDDQHPHVVYDFTESRKRDGPAKFLTGFAGYLHADAYGGYDGIYAGGGAIEVACWAHARRKWFEARKTDPARAHHALALIQKLYHIERDARRA
SDEERHAARQEHSLPILAEFKTWLDAEREQLLPKSPIGQAATYTFNQWTALTRYCESGELKIDNNAAERTMRPVAIGRKNWLFVASRTGGERAAVLMSVV
QTCKRNQVEPWAYLRDVFEQLPSLGENPTRENLDQLLPNQWLKANPNHVWRIQQLREQDDA
Blast result :
Comments
ISBlma9 is 45%(orf1) aa similar to ISRhsp5, 66%(orf2) to ISShes9 (orf2) and 56%(orf3) to ISPre3 (orf3).
References
1] Amann,R., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct Submission GenBank.
2] ISfinder annotation (2008).
2] ISfinder annotation (2008).