ISKpn10

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AJ971342 ND Klebsiella pneumoniae
Klebsiella pneumoniae plasmid pRDDHA
DNA section
IS Length : 1198 bp

Ends


IR Length : 12

IRL : GGTAATCCCCCCGAAAAACCAGGGTGCTCATAAGTAGAATTTTTCCGTAC
IRR : GGTAATCCCCCCATTTTTAGGGGCATTCAAAAGTAGAGGTCATGCGGCCT

Insertion site


Left flankDirect repeatRight flankDR Length
TACCCAAGTGGGGCAGCCCAAGTG4
TGTGCCACTGAAATCACCCGATTA4
GCGCACACCGATCTGTCGCAAGGC4
ACCGTCAGCACCCCCATCAAGATG4
TGGCTGTTCTAACCCATGAC0

DNA sequence

GGTAATCCCCCCGAAAAACCAGGGTGCTCATAAGTAGAATTTTTCCGTACCTTGAACGCAGGAGGTTCTACATGAAAACATCACGCTTTAGCGATACCCA
GATCATCGCCATCCTCAAACAAGCTGAGGCTGGAGCTCCCGTCCCGGAGCTCTGCCGCGAGCATGGCATCAGCACGACCACCTTCTACAAGTGGCGTTCC
AAATTCGGGGGGATGGATGCCTCCCTCATGGCTCGACTCAAGGAGCTGGAGGACGAAAACCGCCGCCTCAAGAAGATGTACGCCGAGGAGCGCCTCAAGG
CAGAGATCATCACCGAGGCCATGGCAAAAAAGTGGTAACGCCATCTGCCCGCCGGGAGATGGCGACCAAAGCCGTGACTCACTACGCCATATCCATCCGT
CTGGCTTGCCGGATCTTCAAGGTCAGTGAAAGCTGCTATCGCTATCAGCCCGTGCTGGTCGATGAAAACCAGGAAATTGCCGATTGGCTGCTACGTATCA
CGGCCAGCCAGCGCAACTGGGGCTTCGGGTTATGCTTTTTGTACCTGCGCAACGTCCAAGGCTTTGGCTGGAATCACAAACGGGTATACCGGATCTATCG
GGCGCTGGAGCTCAATCTGCGGATAAAACCCAGGAAACGGTTGGTTCGGGCTAAGCCAGAGCCGTTGGCGGTTCCCGACCAGCCTAATCAGTGCTGGTCG
ATGGACTTTATGCATGACCAGCTCAGCGATGGCCGCTCGGTGCGGCTGTTTAATGTGATCGATGACTTCAATCGTGAAGCGCTGTGCATCGAGGTGGATT
TCTCCTTACCCGCCTCACGAGTCAAACGGGCGCTGGAGCAGGTGATCACGTGGCGAGGTAAGCCCGCCATTATCCGATGTGACAATGGCCCGGAATACAT
CAGCGAAGAGCTCAAGCGATGGGCGAAACAGGAGGGGATAACCCTGGGCTATATCCAGCCAGGAAATCCGCAGCAGAATGCCTACATCGAACGGTTTAAC
CGCACGGTGCGCTACGACTGGCTGGGCCATTACCTGTTTGAGTCACTGAATGAACTACAGGAGTTTGCCACGAACTGGCTGTGGGTTTATAACCATGAGC
GGCCCAATATGGCGCTGGGTGGCTACACGCCGAAACAGCGTCTGGCACAGGCCGCATGACCTCTACTTTTGAATGCCCCTAAAAATGGGGGGATTACC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
264 bp88 aa72335+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKTSRFSDTQIIAILKQAEAGAPVPELCREHGISTTTFYKWRSKFGGMDASLMARLKELEDENRRLKKMYAEERLKAEIITEAMAKKW

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1032 bp343 aa1281159+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GWSSRPGALPRAWHQHDHLLQVAFQIRGDGCLPHGSTQGAGGRKPPPQEDVRRGAPQGRDHHRGHGKKVVTPSARREMATKAVTHYAISIRLACRIFKVS
ESCYRYQPVLVDENQEIADWLLRITASQRNWGFGLCFLYLRNVQGFGWNHKRVYRIYRALELNLRIKPRKRLVRAKPEPLAVPDQPNQCWSMDFMHDQLS
DGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRALEQVITWRGKPAIIRCDNGPEYISEELKRWAKQEGITLGYIQPGNPQQNAYIERFNRTVRYDWLG
HYLFESLNELQEFATNWLWVYNHERPNMALGGYTPKQRLAQAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1088 bp362 aa721159+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKTSRFSDTQIIAILKQAEAGAPVPELCREHGISTTTFYKWRSKFGGMDASLMARLKELEDENRRLKKMYAEERLKAEIITEAMAKKVVTPSARREMATK
AVTHYAISIRLACRIFKVSESCYRYQPVLVDENQEIADWLLRITASQRNWGFGLCFLYLRNVQGFGWNHKRVYRIYRALELNLRIKPRKRLVRAKPEPLA
VPDQPNQCWSMDFMHDQLSDGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRALEQVITWRGKPAIIRCDNGPEYISEELKRWAKQEGITLGYIQPGNP
QQNAYIERFNRTVRYDWLGHYLFESLNELQEFATNWLWVYNHERPNMALGGYTPKQRLAQAA

 

Blast result :
Comments
ISKpn10 is 94% (ORFA) and 80% (ORFB) aa similar to ISXcd1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Verdet,C., Benzerara,Y., Gautier,V., Adam,O., Ould-Hocine,Z. and Arlet,G. (2006) Antimicrob. Agents Chemother. 50 (2), 607-617