ISfinder Mirror Site

ISPto8

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAPR00000000	ND	Psychroflexus torquis	Psychroflexus torquis ATCC 700755

DNA section

IS Length : 2353 bp

Ends

IR Length : 22/24

IRL : GTATCCACCCCACCAACCACATATTGACCACCTGACTTTCTCTCGATAGC
IRR : GTATCCATCCCACCAACTACATATAGCAAGTCAAACTGATTATGTTTATC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAAAACTGTG	CTTTAAAA	CGTGTAAACT	8
TGGTTTAATG	ATTTTAGA	AGTGTATAAT	8
TGAATTATAT	AGCAAACG	CTGGACTTAT	8
TTTTTTCACT	TACTAAa/tC	GTCATAAAAT	10

DNA sequence

GTATCCACCCCACCAACCACATATTGACCACCTGACTTTCTCTCGATAGCTTTGCTCTAAAATAATATTTATTATGAGCAAACACGAAGAAATGTACAAA
TTGGTAACGGAATATCAAAGTAGTGGCCTTTCGTCAAAAGCCTTTAGCAAAGAAAGAGGGATCAGCCCTTCTACTTTTAGTTACTGGATACGCAAAAAGA
AAGAGGAAGATCACCCGGGCGGTTTTGTGGAAGTAGACACTGGATTGAAATCTTCAGCCAGTGCCATGGAATTTATTTACCCCAATGGGGTCAAGCTTCA
AATGAGTTCACCGGATCTGGCATTAATTGCAAGGTTAGTAAAACTTTACTAATGTTCTCGCTAGGTTCTTCCCATTGTTATCACTTTTACCTCAAGCCCT
GTGATATGCGTAAATCCTTCCATGGCCTAAGCGGACTGGTAAGAAATGAACTGAATAGGGAGCCCACAAGTGGCGATGTGTTCGTTTTTCTTAACCGGAA
TCGCACCCATCTGAAGCTCCTTCATTGGGAACGGGGCGGCTTTGTTTTATACTATAAACGCCTGGAGCGGGGAAGCTTCACTCCACCTGCAATAAGGGAA
GGCCAGACTAGTTTCAGCTGGCCGCAACTGGTGCTGATGATCGAGGGTATTACGGTTAAAAAAAGTGTTCAGAAACTGCGCTATTCACACTAGGAAATAA
TCGTATTTTATCAGTAAAAACAGGGGTTAACGCTAGGTTCAAAGTATCGGTTTTTGTATATTTATCCTATGCAAAATGCCCTGGAAAACCTTACTAAAGA
CCAACTTTTGAGCCTACTTCAGAAGAAGGGAAAGGATGTTGAAAAGGCACACTATAAGATCTTGGATTTGGAGTTTCAACTGGCTCAGTACAAACGGATT
GTTCACGGACAGAAAAGAGAACGCTTCGAGGGTGATAAGGATCAAATGAGCCTTCCCTTTGAGATGGAGCCAGAAATAGCACAAAGGCAGGAAGAAGAAG
TAAAGGAAAAGCTTACCTATGAGCGCCGAAAAAGGAAATCTGCTCATAAAGGTCGAGTACCGCTTCCGCAGCATCTTCCCGTGGAAGAAATCAAGATCTA
CCCTGAGGGCGATTTAAGCGATATGGTCTGTATTGGAGAAGAAATCACCGAGGAATTAGAATATGAGCCTTCCAAATATTACATCAAACGCTACATCCGC
TATAAATACGCTCCTCGGAATAAAGAAGGAGTTGTAATAGGAGCGCTTCCAGAGCGAGTTATTGAAAAAGGAATACCGGGAGCCGGGCTTTTAGCTTCCA
TTCTGGTCGATAAGTATGAAGACCATCTCCCTCTTTACCGCCAGCTTCAGCGTTTTAAAAGAGCTGATATTCCTATTGCCTCTTCCACTTTGGAAGGCTG
GACCAGGCAAAGCCTTAAAATATTGGATATTCTTTATCAGCATTTGCTGGAAGATATTCGGTCAAAGGGGTATCTCCAGGCCGATGAGTCCCCCATCAAA
GTCATCGATAAGAAAAAGAAAGGCACCACCCATCAGGGATACTACTGGGTGTACCATTGTCCTATAGATAAAACGGTGCTTTTTGATTATCAGTCGGGGC
GCAGCGCCCAGGCTGCAGCGCATGTATTATCAGGATTTAAGGGCTACCTGCAAAGTGATGGGTATTCTGTTTATGATAAAATTGGAAAACGCGAAGGGGT
TACTCACCTTAATTGCTGGGCTCATGCCCGAAGGGAATTTGATAAAGCCCGGGACAATGATAAACTAAAGGCAGAGATAGCGCTTACTTTTATCCAGAAG
CTCTACAAAGTCGAGGCTAAGGCACGGGAGAATAATTTAAATCCAAAAGAGCGAAAAAAGCTTCGGCTGGACGAGTCCTTGGGGATCATTGATGAATTTG
GAAAATGGATGGTAAACCAGCTAAAGGATCACCATATCCTGCCAAAAAGCACAATAGGTAAAGCTATGAGTTACAGCCTGAACCGGTGGGACCAGCTCAG
TGCCTATTTATATGATGGGGTTCTCGAGATAGATAATAATCTCACTGAAAATGCTATAAGAAAATTAGCTTTGGGACGTAAGAACTATCTCTTTGCAGGC
TCTCACGATGCGGCACAACGAGGGGCAATTATGTATTCCTTCTTTGCAATCTGTAAAAAACACGAGGTGAACCCTTATCAGTGGTTAAAGTACACTTTGG
AAAATATTATGTCCATCAACCACAAGAATATTAAAGATTTGTATCCCCAGAATTACAAAAAACTACAGAATACATAAGAGAAGTTTTTTACCCGGAATTA
CCTGATAAACATAATCAGTTTGACTTGCTATATGTAGTTGGTGGGATGGATAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
279 bp	92 aa	74	352	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MSKHEEMYKLVTEYQSSGLSSKAFSKERGISPSTFSYWIRKKKEEDHPGGFVEVDTGLKSSASAMEFIYPNGVKLQMSSPDLALIARLVKLY

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
288 bp	95 aa	406	693	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MRKSFHGLSGLVRNELNREPTSGDVFVFLNRNRTHLKLLHWERGGFVLYYKRLERGSFTPPAIREGQTSFSWPQLVLMIEGITVKKSVQKLRYSH

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1509 bp	502 aa	769	2277	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MQNALENLTKDQLLSLLQKKGKDVEKAHYKILDLEFQLAQYKRIVHGQKRERFEGDKDQMSLPFEMEPEIAQRQEEEVKEKLTYERRKRKSAHKGRVPLP
QHLPVEEIKIYPEGDLSDMVCIGEEITEELEYEPSKYYIKRYIRYKYAPRNKEGVVIGALPERVIEKGIPGAGLLASILVDKYEDHLPLYRQLQRFKRAD
IPIASSTLEGWTRQSLKILDILYQHLLEDIRSKGYLQADESPIKVIDKKKKGTTHQGYYWVYHCPIDKTVLFDYQSGRSAQAAAHVLSGFKGYLQSDGYS
VYDKIGKREGVTHLNCWAHARREFDKARDNDKLKAEIALTFIQKLYKVEAKARENNLNPKERKKLRLDESLGIIDEFGKWMVNQLKDHHILPKSTIGKAM
SYSLNRWDQLSAYLYDGVLEIDNNLTENAIRKLALGRKNYLFAGSHDAAQRGAIMYSFFAICKKHEVNPYQWLKYTLENIMSINHKNIKDLYPQNYKKLQ
NT

Blast result :

Comments

ISPto8 (orf2) is 65% aa simlar to ISBvu3 (orf2).
ISPto8 (orf3) is 56% aa simlar to ISBvu3 (orf3).

References

1] ISfinder annotation (2008).
2] Bowman,J., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct Submission GenBank.