ISfinder Mirror Site

ISDpr4

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAQF00000000	ND	delta proteobacterium	delta proteobacterium MLMS-1

DNA section

IS Length : 2431 bp

Ends

IR Length : 19/20

IRL : GTAAGCGCTCAGCGAACCCCTTCTTTTTTTGTCTCCCCCACCATGGCAAT
IRR : GTAAGCGCTCAGCAAACCCCAGTCACCGGGCAACCACGAGGCTGCGGTCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCACGGTGTC	CCAGGCATTACAGC	TGCTGACCCC	14
CCTTATTTGC		CAAAATTCAG	0
GAGGAGCAAG		CCACCAAAGG	0
TTCGACAAAC		TTTGGTGGGG	0

DNA sequence

GTAAGCGCTCAGCGAACCCCTTCTTTTTTTGTCTCCCCCACCATGGCAATATCTCCCCCGGTTGGCTGGCCGGGATATCCTCGGCCGCATTTTATCCGAG
GAGGAGATCATGGACAAGAACGGCAGGAGGAGCAAGCGTCGTTTCTGGGCCGCCCATGTGGCGGCCTGGGAAAAGAGCGGCCTGACCCAGACCGCGTATT
GCCGGGAGCACGGCCTGAGCCGGCACGCCTTTGGCTGGTGGCGGCGCAAATTTAGAGACCAGCCGGCAAGCGAGCAACCGCTGCTGGTACAGGTACCAAC
CGTAACCCGCCCGACAGCGGCCACCGCGGCTGCTGATTTTTCCGGCTTGCGGCTGTTGCTGCCCCGGGGGCTGCAGCTGGAAATCAACCAGGGGTTCGAT
GCCGCCACCCTGGGGCGGGTGATCGCCACCTTGGAGGCCGGCCAATGATCCCCGCCGAATTCGCTATCCAGGTGTATCTGGCCTTGGGCAGCACCGATAT
GCGTAAGAGTATCGACGGCTTGGCGCTGCTGGTCTCGGAGCAGCTGGGTTTGAACCCACTCAGTGGCCAGCTTTTCGCTTTCAGTAACCGCCGCCGGACC
ATGGTCAAGATCCTTTACTGGGATCGCAACGGTTTTTGTCTGTGGCAGAAGCGCCTGGAAAAGGAACGATTCCGCTGGCCCTGTAGCCGGCAGGAGGTTT
TGACCATCGGTCGCCGGGAATTGAGCTGGTTGCTCGACGGCCTTGAATTGGAGCAGCAACGGGCCCATAAAAAACTGGAATACAGCCGGATTTTTTGATG
CTAGAGATAGATATTCTCTTGACATTTCAGGCAGATGGATTACTATGACGGCATGCCTTCGATCCCCGCCGACATACCCGAAAATATCCGGGCCTATATC
CAGCAGCTGATCGCTGCCCATGAACAGGAGCGTGAACTCTTGTTGGAGCAGATCAAGCTGCTGCGGGCCCAGCTGTTCGGGCGTAAAAGCGAGCAGGTGG
CGGATATCTCGTCGCCGCAGCTGCCGCTTTTTGATGAGCAGGCGGCTGCCGGCGACCCAGCTGAAGAGGCGTACGAGCCTGAAGTTCAGGTGGACTCCCA
CAGCCGCCGCCGGAAGGGGCGCAAGCCACTGCCGGAAGATCTGCCCCGGGTGGAGGTGGTCCATGATGTTGACGCCGAGGCCAAAACCTGCGCTTGCGGT
TGCGAAAAGAGCCGGATCGGCGAGGATGTCTCCGAGCAGTTGGATATGATCCCGGCCAAAATGCGGGTAATCCGCCATGTCCGCCCCAAGTACGCCTGTC
GGCAGTGCGAAGGGGTGGAAGATGAGGGGCCGACCGTGGTCATTGCCCCGCCCCCGGAGCAGATGATCGCCAAGAGTATCGCCAGCCCCGGCTTGCTGGC
CCAGGTGTTGACCGCCAAGTTTGCCGATGCCCTGCCTTTTTATCGCCAGGAAGGGCAGTTTACCCGACTGGGAGTGGAAATCGGCCGGGGTACCATGTGC
GGTTGGGCCATGCAGGTGGCCGAGCGCTGCGGGCCGCTGCTTAGCTTGATGCAGGAGGAGATTCGTTCCGGGCCGCTGATCAATGTCGACGAGACCACCG
TCCAGGTGCTGGCCGAAATCGGCCGGGCCGCCAAAACCAAGTCCTACATGTGGGTCTTTCGCGGTGGTGCGCCCGAACGTCCGGTTTTGGTGTTCCAGTA
TCATCCGACCAGGAGTGGCGATCCGGCCGCAGTGTTCCTCCGTGGTTACCGGGGCTGTGTGCAGACCGACGGCTATACCGGCTATGACTTCCTTGATCAT
CAGCCGGGGGTGATTCATGCCGGCTGCTGGGCCCATGCCCGGCGCAAGTTTAACGATGTCCTCAAGGCGGCCGGTAAGTCTGCCAAGCGGGACGGTATTG
CCGGCGAGGCCCTGGACTGGATCGGCAAATTGTACAAAATCGAGGCGACCATGCGGAAGGCCGAGCTTGCTCCGGAGGCGATCCACCAGCGGCGCCAGGA
GCAGGTGGTGCCCTTGCTGGCTGATTTCCGGCAATGGCTGCTGGCCCGCCAGCGGGAGGTCCCGCCCAAGAGCTTGCTGGGCAAGGCCATTGCCTATACC
CTGAGCCAATGGAAAAGATTGCGACGTTACGTTGAGCATGGCCTCCTGCGACTGGATAACAACTTGGCGGAAAACGCCATCCGCCCCTTTGTCGTAGGTC
GGAAAAACTGGCTCTTCTCCGGCACCGCCCAAGGAGCCAAGGCCAGTGCCGCAATTTACAGCATTATCGAAACGGCCAAAGCCAACGGCCTGGAGCCTTA
TTGGTACCTGCGCGCTTTGTTCGAACGACTGCCGGCGGCCAAAACCCCGGAACAGCTCAAGGCGCTACTGCCCCAGTACATCGACCGCAGCCTCGTGGTT
GCCCGGTGACTGGGGTTTGCTGAGCGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
339 bp	112 aa	110	448	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MDKNGRRSKRRFWAAHVAAWEKSGLTQTAYCREHGLSRHAFGWWRRKFRDQPASEQPLLVQVPTVTRPTAATAAADFSGLRLLLPRGLQLEINQGFDAAT
LGRVIATLEAGQ

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
354 bp	117 aa	445	798	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIPAEFAIQVYLALGSTDMRKSIDGLALLVSEQLGLNPLSGQLFAFSNRRRTMVKILYWDRNGFCLWQKRLEKERFRWPCSRQEVLTIGRRELSWLLDGL
ELEQQRAHKKLEYSRIF

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1575 bp	524 aa	835	2409	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDYYDGMPSIPADIPENIRAYIQQLIAAHEQERELLLEQIKLLRAQLFGRKSEQVADISSPQLPLFDEQAAAGDPAEEAYEPEVQVDSHSRRRKGRKPLP
EDLPRVEVVHDVDAEAKTCACGCEKSRIGEDVSEQLDMIPAKMRVIRHVRPKYACRQCEGVEDEGPTVVIAPPPEQMIAKSIASPGLLAQVLTAKFADAL
PFYRQEGQFTRLGVEIGRGTMCGWAMQVAERCGPLLSLMQEEIRSGPLINVDETTVQVLAEIGRAAKTKSYMWVFRGGAPERPVLVFQYHPTRSGDPAAV
FLRGYRGCVQTDGYTGYDFLDHQPGVIHAGCWAHARRKFNDVLKAAGKSAKRDGIAGEALDWIGKLYKIEATMRKAELAPEAIHQRRQEQVVPLLADFRQ
WLLARQREVPPKSLLGKAIAYTLSQWKRLRRYVEHGLLRLDNNLAENAIRPFVVGRKNWLFSGTAQGAKASAAIYSIIETAKANGLEPYWYLRALFERLP
AAKTPEQLKALLPQYIDRSLVVAR

Blast result :

Comments

ISDpr4 (orf1) is 44% aa similar to ISAeme5 (orf1),
ISDpr4 (orf2) is 65% aa similar to ISAeh1 (orf1),
ISDpr4 (orf3) is 63% aa similar to ISPpu13 (orf2).

References

1] ISfinder annotation (2008).
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct Submission GenBank.