ISfinder Mirror Site

ISBf10

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006347	ND	Bacteroides fragilis	Bacteroides fragilis YCH46

DNA section

IS Length : 2939 bp

Ends

IR Length : 20/21

IRL : GTAAGCATTCGATAAACTCCCCCTTTCATTCTAAAAATGTGATATTACTT
IRR : GTAAGTATTCGATAAACTCCCTCGTATATTGGGTAACGTATGTTCATAAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGGAGATACC	TTTGCTTT	CGTCTTTAGT	8
GAAAGACTGT	TTTATTTC	AATCTTGTTT	8
TTGATTGTAA	AATACTTC	ATTGCTTCTT	8
TGATGTCTTA	CCTTGAAC	GCAATTCACC	8

DNA sequence

GTAAGCATTCGATAAACTCCCCCTTTCATTCTAAAAATGTGATATTACTTTTGTAATCAAAGTAAAAAAGCTCAATTGTTATGTCACATCAATGGACCAT
GGAAGATTTTGAATCTATTTATTCCCGTTTCAAGTCGAGCGGACTGTCAGTTATGGATTTTTGTTCGAATGAATGTATTCGTCCCAAACGTTTCTACGAG
TGGCGTTCCAAGCTGTTGCGCAAAGGCGGCTTTATCCCGGTAAAGGTAAACAGTAAGGGCCAGGTCAGTCTTCCCCATAAGGAGAAATCCCTGCTGTCTG
CCCCTCCGGTCAGCCCGTCGCCAATTCCCCAGCCGCTATGTGAGATCTCCTATCCCAATGGCGTCACAGTCCGCTTGAACAGCCCTTTGTCACCGGAGGT
ATTGCAAACCCTGATATTTTTGAATTCAAACCGTTAGCCTATGTTTTCTTTGAATGAATCCAACAGATATTATCTTTACCCGTATCCGACAGACATGCGT
AAGAGTTTTTATACGCTTAGCGGCATCGTGACCAACCAGATGGGAAAGAATGTACGGGACGGTGACGCTTTCATTTTTATCAATGCGAATTGTACCTGTA
TGAAAATCCTCCATATGGAATATGGTGGTCTGGTGATATACCATATGAGGCTGGAACATGGGCACTTCCATCTGCCGGTCATAAATACGGAGGAAGGTCG
GATTAAAGCGATTGAAACCTTCTGGAATGACCTTGTGATGATGGTTCAAGGAATGGACGGCAGCAAGGTCAGGCGTTATAAAAGGAGTGGTTTCCATGGG
TTGTAGTTGTAACAAATTATAACATATACAATGCCGTATTCCGGCTGTTTTTTAGTATCTTAGCATCATATTTAAACGATTATGCCAACCGATAAGGAAT
TACTGATAAAGGATCTCATGCACAAATGCGACTGTCTGTATAGAGAAAACAGCCGGTTGAAGGAGATGGTGTCCACGCAGCCCCTTAAAGCTGCGGACAA
AGAGGTGTATGAGGCTTTATTGTCTGACAAGGATGCCATAATCGCCCAAAAGGAAGCAAAAATCAACAGTCTGGAGCAACGTGTATCCTATCTTGAACGG
CAGTTGTACGGTAAGAAGGCGGAAAAGTTCATAAAGCCTGACGCCCAGGACCGCTGGCTGGATTTTGAAGGCTTTGACATGCTTCCCCAGGAGGCTGAAG
CCGCAGAAGAAGCAGAAAAGGAGTTGAAGGCTACCAGAGAAGCGATCATCGCCCGTAAAAAAGCGGGGAAGCAGCATCCTGCGAGAAAATCCCTTCCGGA
GAATCTTGAGCGTGAGGTGGTCCATATATATCCCGAAGGGTATAATCCGGAAGAGTGGACGCTCCTTCCCGGAGAGGAAGTGACCGAGATCCTTATGCAC
GAGCCCGAGAAGTTCTATATCCGCAGGATAGTGCGCCATACAGCCAAACGGAAGGGTACAAACGAGTTCAAGACCGGCCCGCTTCCCGTCATGCCAATAG
CAAAGAGCTATGCCTCTGCCTCATTGCTGGCAGACATGATGATAGGGAAATATGTGGATCACATACCGTTCCACAGGCAACTGGAACAGTTCAAACGTGT
GGGGGTACATCTCCCCGCGTCAACGGTCAACGACTGGTTCAAGGATGTGGCGGATTTGCTAAGGCCCCTTTACTTCCGATTATGGGAGCTGGTGATGCAG
ACTGACTACATACAGTCGGATGAAACGACAATCCCCGTGATGAATGACGAGAGACACAAGACGGTCAAAGGTTATATCTGGCTTGTGCGCAGTGTCATGA
CCGGACGTCAGTTCTTTTACTATGACAAGGGTTCCCGAAGTGGAAAGGTGGTGCTGAAACTCTTCGGCAAGTTCCGGGGAGCCATACAGACGGACGGATA
CGAAAGGTACGAGATGCTGGACGCCAAGAAAGGTATTATCCTTCTTGGCTGTTGGGCCCATGCACGCAGACATTTTTGGGAGGCAAGAAAGAATGACATG
CAGCGTGCCGACTATGCGCTCGCACAGATACAGTTGCTTTATGACGTGGAGCGTAAGGCTGACGATGAACGCCTGACTTACGAACAGAGGGCTGAACTTA
GGGCACGTCTTGCATATCCCATACTTGTGCGCTTCGAGAAATGGCTGGTCAATGAATATCCCAAAGTAATGAAAGACAGTCCGATCGGAAAAGCCATAAA
ATATACATACGGAAGGTTCGACAAACTCTCCAGGTACCATCTGGACGGTCGTTACAGACCGGATAATAACGAGATAGAAAATAAAGTGCGGCCTGTCGCG
TGCGGCAGACGTAATTACCTGTTCTGTGGCAATAATGATGCCGCTGAAGATGCGGCGGTGCTCTACTCGTTCTTCGGCTGCTGCAAGGCTGCCGGGGCTG
ACTTCCGCACGTGGCTGATCTACTTCCTTGAACATATACATGATTATGACGATGACTACTCGATGGATCTGGCCGAATTGTTACCTGACAATCTGTTATC
CAAGGGCAAGATATTATCCGTTACGTCACCGGAATCTCCCAAGAAAGACTCCTGATACCTTCGTAAATCTCGCCTAAACACGGGAAAACTACCGTTTTAT
GCCAATCAAGACGGGAAAATAGGGAATAAAGTGCCGTTTTTATCTTCATTATCCCCGATAAATAGGGAAAATAAGGGAAAGAATACCCTTTTATACCAAT
TATGGCGGGAAAGTAGGGATAAAAATATCGCTTTATCACATTTGTCACAGGAAAATAAGGGGCAAAGGGGAAAAACTTCCTTTTATCCGCTCCATTAGTA
GGGATAATATCAATTACTATAGAACCCGCACATCTTTGGGTTGAATTGATGTAACAATAAATCATAGAACGATAAAATGAATAAACGACATTATGAACAT
ACGTTACCCAATATACGAGGGAGTTTATCGAATACTTAC

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
357 bp	118 aa	81	437	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MSHQWTMEDFESIYSRFKSSGLSVMDFCSNECIRPKRFYEWRSKLLRKGGFIPVKVNSKGQVSLPHKEKSLLSAPPVSPSPIPQPLCEISYPNGVTVRLN
SPLSPEVLQTLIFLNSNR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
366 bp	121 aa	441	806	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MFSLNESNRYYLYPYPTDMRKSFYTLSGIVTNQMGKNVRDGDAFIFINANCTCMKILHMEYGGLVIYHMRLEHGHFHLPVINTEEGRIKAIETFWNDLVM
MVQGMDGSKVRRYKRSGFHGL

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1674 bp	557 aa	882	2555	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPTDKELLIKDLMHKCDCLYRENSRLKEMVSTQPLKAADKEVYEALLSDKDAIIAQKEAKINSLEQRVSYLERQLYGKKAEKFIKPDAQDRWLDFEGFDM
LPQEAEAAEEAEKELKATREAIIARKKAGKQHPARKSLPENLEREVVHIYPEGYNPEEWTLLPGEEVTEILMHEPEKFYIRRIVRHTAKRKGTNEFKTGP
LPVMPIAKSYASASLLADMMIGKYVDHIPFHRQLEQFKRVGVHLPASTVNDWFKDVADLLRPLYFRLWELVMQTDYIQSDETTIPVMNDERHKTVKGYIW
LVRSVMTGRQFFYYDKGSRSGKVVLKLFGKFRGAIQTDGYERYEMLDAKKGIILLGCWAHARRHFWEARKNDMQRADYALAQIQLLYDVERKADDERLTY
EQRAELRARLAYPILVRFEKWLVNEYPKVMKDSPIGKAIKYTYGRFDKLSRYHLDGRYRPDNNEIENKVRPVACGRRNYLFCGNNDAAEDAAVLYSFFGC
CKAAGADFRTWLIYFLEHIHDYDDDYSMDLAELLPDNLLSKGKILSVTSPESPKKDS

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
168 bp	55 aa	2631	2798	+	No

ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

MPFLSSLSPINRENKGKNTLLYQLWRESRDKNIALSHLSQENKGQRGKTSFYPLH

Blast result :

Comments

ISBf10 (orf1) is 43% aa similar to ISCro1 (orf1).
ISBf10 (orf2) is 48% aa similar to ISCro1 (orf2).
ISBf10 (orf3) is 48% aa similar to ISPre3 (orf3).

References

1] Kuwahara,T., Yamashita,A., Hirakawa,H., Nakayama,H., Toh,H., Okada,N., Kuhara,S., Hattori,M., Hayashi,T. and Ohnishi,Y. (2004)Proc. Natl. Acad. Sci. U.S.A. 101 (41), 14919-14924.

2] Hattori,M., Yamashita,A., Toh,H., Oshima,K. and Shiba,T. (2004) Direct Submission GenBank.

3] ISfinder annotation (2008).