ISfinder Mirror Site

ISBthe5

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_004663	ND	Bacteroides thetaiotaomicron	Bacteroides thetaiotaomicron VPI-5482

DNA section

IS Length : 2537 bp

Ends

IR Length : 21/24

IRL : GTAAGCCCCCGATAAAGTACCCGTTGTCTAGTATCTTTTTTCGCTCGATC
IRR : GTAAGCTCCCGATAAAGTCCTCGTATAAATTATAGAATCGCTATATACAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TTATAAAATG		AGTTGAATAG	0

DNA sequence

GTAAGCCCCCGATAAAGTACCCGTTGTCTAGTATCTTTTTTCGCTCGATCTTTGCGTAAAAAAGATTATGCGAAAAATGAGCAAAGAAGAATTCTTAGAA
ATTCTATCCCGTCAGCAACGAAGCGGTTTGACGATAAAAGACTTCTGTGTCAATGAAGCCTATACCGAATCTAGTTTTTATTATTGGAAAGGAAAGTTTG
GTCTCTCACGACCTTATCATGGGGAAAGATCCTCTTCCGGAGAGTTCGCTCCTATCAATTTGACCTCCCCGTCCACATCCAACCCGTCTTATGATAGAGT
AGCTATGGGATCCGGGGAGATTCAAATAGAGTTTCCTGGTGGCATAATAGCCCGCTTCAGTGGTATGGCTGAATCCCATGCCGCCATGCAATTACTCACT
CAAATTTACGGTCATCATGTTTTGCCTGAATGATACGATGCGATATTTCCTTTGCCCGGGTAAGACAGATATGCGCAAAGGCATGAATTCATTATGTGGA
GTCATTCATAACAAGATGGGCTACGACGTCCGTTTAGGTGACGTATTCATTTTCATAAACCGGCAGCGGACCACGATGAAACTTCTGCATGCGGAAGATG
GCGATCTTGTCTTGTATATAAAAAGGCTGGAGGAAGGAACCTTCCGCTTGCCCGAATATGATCAGCAAAGCAGATCATACCCCATGGAGTGGCGTGATCT
GGTCATGATGGTGAAAGGAATCAATAACGGATCCGCTAAGAGGCTCAAGCGATTGAAGGCGTTACGAAAAAGTGATATATAACAGTGAAACTCAATGGAC
CAGACCTTGTTTAAGTAAGAGTTTTTTCTTATTTTTGTATTGTTCAAAATCCTCATAAATAATGACTCGGACAGAAACATTAGAACTTTTGGTAGCCACA
CTCCAACAGACTAACGCCAGTCAGTCTGAGTCCATCAGACAGCTGACCCGGCAGAATGAACAGTTACAAAACAAACTGGATGAACTGCTGGCTCAGGTAG
CATGGCTGAACCGACAACTTTTTGGTCGTAAGAGTGAAAAGTTATCCCGTCTAGACCCAAATCAGCTATCCCTGTTTGAACAGCCGGTCCAATCCTTAGA
ACCGGAACCTTTGGAAGAAACTGTAGTTGAACAAACTACAACACCTATGGTTACTAAAAAGAAAGAACGGCAGAACCGTAAACTGTTGGAAGGACTTCCG
GTTGTGGAAGTTGTCATAGAACCGCAAGACCTGGATCTTACAAAGTATAAACGGATTGGCGAGGAACATACACGTACACTTGAGTTTGAACCGGGTAAAT
TATATGTAAAGGAAATCATACGTCCCAAATATGGACTGAAAGACAATACCGCTTTACCCCAGGAATATCAGGATGGTATCGTAATAGCCGATCTTCCACT
GTTGCCTATTTATAAGGGACTTCCGGGTGCCAGTCTGCTGGCAGAAATTCTCCTGCAAAAGTATGAGTATCACGTTCCATTCTACCGTCAGATACGGGAG
TTCCATCACTTAGGTTTGAAAATCCCGGAAAACACGCTTCATGGCTGGTTCAAACCCGCCTGTGAGCTGCTAAAGCCTTTGTATGATGAACTCAAGAAGC
AGGTATTGGCAGTCGATTACATCCAGGTGGATGAAACCACATTGCCTATCATCAACAAGCAAAGCCACAAAGCTGTCAGGGAATATCTGTGGATGGTCAG
GGCGGTAACCAGCGGATTAGTATTCTTTCATTATGATGACGGTTCCCGTTCACAGGAAACGGCACGGAACTTATTGGAACCATTTAAAGGATATCTTCAA
AGTGACGGTTATGCGGCCTACAACGTCTTTGAAGGTAAGGAAAGGGTGTGCCTTGTGGGTTGCCTTGTCCATATCAGGCGTCACTATGAGACCGCCAAAG
AGGAAAATAAGTCTCAGGCTAAGTACGTTCTGGCCAAAATACAGGAACTCTACCGGATAGAGCAGGCGGCCGACATACAGGGAATATCCCCTGAAATGCG
AATGTCAAAAAGGCAGAAACAGGCTCTTCCTATTCTTGACGAGCTGGAACAATGGATGGAAACAACCTATCCGAAAGTGCTTCCTAAAAGCCAGATGGGA
CAAGCCATTGCCTATGCCTATACCCTTTGGCCACGCATGAGAAATTACCTCAAAGACGGCAAGCTCAAAATTGATAATAATCTGGCTGAAAATGCAATCC
GCCCAATAGCTTTATCGAGAAAAAACTTCTTGTTTTGTGGAAATCATGAAGCGGCGGAAAACACAGATGTCATCTGTTCATTATTGGCATCATGTAAAGC
ATCTCAAGTTAACCCTAGAGAATGGCTTACCGAAATAATTGCCTGGTTGCCATATTACACAAGGGATAAGGGAAAAGACCTAAAAGAGTTGCTACCCAAT
TACTGGAAACTGAGAAGATCCAAAGAAATCTAACAATACTCTAATGAAAAGCATATTATAAACAGAGAATCGCTATAAACTTATTAGATGTATATAGCGA
TTCTATAATTTATACGAGGACTTTATCGGGAGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
366 bp	121 aa	68	433	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MRKMSKEEFLEILSRQQRSGLTIKDFCVNEAYTESSFYYWKGKFGLSRPYHGERSSSGEFAPINLTSPSTSNPSYDRVAMGSGEIQIEFPGGIIARFSGM
AESHAAMQLLTQIYGHHVLPE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
345 bp	114 aa	438	782	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MRYFLCPGKTDMRKGMNSLCGVIHNKMGYDVRLGDVFIFINRQRTTMKLLHAEDGDLVLYIKRLEEGTFRLPEYDQQSRSYPMEWRDLVMMVKGINNGSA
KRLKRLKALRKSDI

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1572 bp	523 aa	862	2433	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTRTETLELLVATLQQTNASQSESIRQLTRQNEQLQNKLDELLAQVAWLNRQLFGRKSEKLSRLDPNQLSLFEQPVQSLEPEPLEETVVEQTTTPMVTKK
KERQNRKLLEGLPVVEVVIEPQDLDLTKYKRIGEEHTRTLEFEPGKLYVKEIIRPKYGLKDNTALPQEYQDGIVIADLPLLPIYKGLPGASLLAEILLQK
YEYHVPFYRQIREFHHLGLKIPENTLHGWFKPACELLKPLYDELKKQVLAVDYIQVDETTLPIINKQSHKAVREYLWMVRAVTSGLVFFHYDDGSRSQET
ARNLLEPFKGYLQSDGYAAYNVFEGKERVCLVGCLVHIRRHYETAKEENKSQAKYVLAKIQELYRIEQAADIQGISPEMRMSKRQKQALPILDELEQWME
TTYPKVLPKSQMGQAIAYAYTLWPRMRNYLKDGKLKIDNNLAENAIRPIALSRKNFLFCGNHEAAENTDVICSLLASCKASQVNPREWLTEIIAWLPYYT
RDKGKDLKELLPNYWKLRRSKEI

Blast result :

Comments

ISBthe5 (orf1) is 84% aa similar to ISBthe6.
ISBthe5 (orf2) is 51% aa similar to ISCro1 (orf2).
ISBthe5 (orf3) is 50% aa similar to ISPpu19 (orf3).

References

1] Xu,J., Bjursell,M.K., Himrod,J., Deng,S., Carmichael,L.K., Chiang,H.C., Hooper,L.V. and Gordon,J.I. (2003) Science 299 (5615), 2074-2076.

2] Xu,J., Bjursell,M.K., Himrod,J., Deng,S., Carmichael,L.K., Chiang,H.C., Hooper,L.V. and Gordon,J.I. (2002) Direct Submission GenBank.

3] ISfinder annotation (2008).