ISfinder Mirror Site

ISBrsp6

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009485	ND	Bradyrhizobium sp.	Bradyrhizobium sp. BTAi1

DNA section

IS Length : 3112 bp

Ends

IR Length : 24/29

IRL : GTAAGCGTGGCGCCGAGGCCCTTTGGATTTTTTGTGCGGAGTCTTGAGAG
IRR : GTAAGCGTAGCTCTGCGGCCCTTTTGATTGGCGGTTGACGCCTATTTTGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGTCCGCTGC	CATGGATC	TCAAGTGCC	8

DNA sequence

GTAAGCGTGGCGCCGAGGCCCTTTGGATTTTTTGTGCGGAGTCTTGAGAGCACCGTTAGAAGAGTCATCTTCTGCAAGGGTGCTCATAATGGACGACCAT
TCGGACGACATAAGACGACCGTTGTCACCGCGTATTGAAGTGTTCGCAGGCGCAGGTCGCAAGCGCTGGCCGGATGATTTGAAGGCGCAGATTGCTGCGG
AAAGCCTCGAGCCGGGGGCGGTTGTCACAGACGTCGCCCGTCGCCATGGCTGCCGGCCCCAGCAGGTGCATGACTGGCGGCGCCGGGCGCGTTTGGGCCA
ATTGGTATTGCCGGCGTCGGCGGACACGCTGTCGTTCGTGCCGGTGATGTCGGAATCGGCGTTGCCTGCGGCCGCAGACCCCTCCGGGTCGCCGGAAGCC
GCCGTTGTGACGGTTGAGTTCCAGGGCGCGCGCGTGGAGGTGCGCGGGGCGCCTGGCCTTGCTGTATTGAGCGATGTGTTCATTGCGCTCCGCCGGACAC
GTTCATGCTGACGCCACCCGCGAGCCTCATGATCTACGTGGCGACGCAACCTGTCGACTTCAGGAAGGGAGCGGAAGGCTTGGCGCTGCTGGCGAAGGAG
ACGCTGGGCCATGACCCGATGAAGGGCGTGGCGGTGGTGTTCCGTGCGAAGCGCGCCGATCGGGTGAAGATCGTCGTCTGGGATGGCAGCGGCCTTGTGA
TGTATTGGAAGCGGCTCGATGGCAGCGGCTTCAAATGGCCTCCCATCGTGGCCGGCGTGATGCGGATGAATGCCGCCCAGCTGTCCGCGCTTCTGGCCGG
CATGGATTGGACGCGCATGCACGCGCCTCGAATCCCGCAGCCGAAAGCGCTTGCGTAAAAATGCAAATCATCGCTGCATCGTGACGCGAAGCATGGCAAG
CTCGGGCCAATGAGTGATTTGCCCGATGAGCTGCCGAGCGATCCAGCCGAGTTGCGTGTCTTTGCCACGGCTCTGCTGGATCGCTGTGCCCGGCTCGAGC
GGTTGCTCAAGCTTGCAAAAGATGCGCAGTTCGGTCGGTCCTCGGAAAAGCTGGACGCTGATCAGCTACAGCTTGTTCTGGAGGACGTCGAAGGGGCCGT
CGCGGCTCTCGAAACAGCCGAGGATCGCGCTAATCCCAGAACCTCCGAGAAGAGAGCGGCCGAGCGTAGAACCAACCGTGGTCAGCTTCCGGAGCATTTG
CCGCGCATCGTCGAGACCCTGATGCCGGCGGAGACTTGTTGCCCGTCTTGTAAGGGCGACCTCTTTGAGATTGGTCGGGATGAGAGCCAGAGGCTGGACG
TCGTTCCGGCGCAGTATCGCGTCATCGTCACCCGCCGGCCCAAGCTGGCCTGCCGTGCCTGTCATGGCGTCGTCCTCCAGCACGCTGCTCCCGAGCGACT
GATCAGGGGTGGATTGCCCACCGAGCGGCTGGTCGCGCATGTGATCGACGCGAAGTATCATTGGCATTTGCCGCTTTACCGCCAGGCGCAGATGCTGGCG
ACGCACGGTATCGCCATCGACCGTTCCACGCTCGCCTTCTGGGTCGGCTATGCCGCCCAGGAATTGAAACCTTTGTGGCATCGTCTGCGCCAGCTTCTGC
TCGCCTCCTCGAAGCTCTGTGTCGATGAGACGCCGGCGCCGGTGCTGGATCCGGGGCGCGGCAGGACCAAAACCGGCTACTTCTGGGCGCTGTCACGCGA
TGACAGGCCTTGGGCCGGACCTGATCCGCCTGGTGTGGTCTATGCCTATGCACCGGGCCGCGGCGCCGTTCATGGCTTGCGGCTGCTTGAGGGCTATCGC
GGCATCATCCACTGCGACGGCTATCAGGCCTACAAGACCATGACCCGAGAGACGCGTGCGGACGCCTTGTCCGGGACGCTCGCCTTCTGCTGGTCGCATC
TGCGGCGCCAGTTTGTGAAGATCGAGCGGGAGGCTGCGCCGGCGCCAGCTCCGGTTGCCCGCGAGGCTCTTGAGCGCATCGCTCAGCTTTACGCGGTCGA
GAAAGCGCTCCGCGGCCGATCAGATGCCGAGCGCCGTGCTGGCAGGCAGGCGCACGCCCGACCTCTGGCTGCAGCCTTGAAGCAGTGGTTTGAGGCCAAG
CTTGATCACCTTGCGCAAAAGAGCGACACGGCGAAGGCTTTACGTTATGCGCTGCGTCATTGGGACGGACTGACGCTCTACCTTGATGACGGGCGCATCG
AGATGGACACCAATGCGGTCGAGCGAGCGATGCGGCCGATCAAGCTCAACGCCAAGAACTCTCTTTTTGCCGGTTGCGACGAGGGCGCCGAGAGTTGGGC
CGTCCTGGCTTCGTTAATCGAGACCTGTAAGCTCAATAGCGTCAGTGCCGAGCACTGGCTTGCTGACGTTCTCGCGAAGCTCGTCAATGGTTGGCCTGCG
ACGCGACTGGACGAACTGCTTCCCTGGGCATCGATCTACACGATGCATGCGCACGATCGGCTGGCGGCATGAGCCTGAACACGACCGCGGTCCGAATCAA
GGTGACCCTCAAGGACGTGAAACCGGAGGTGATGCGTCGCCTTGTTGTACCCGTCACCTTACGCCTTGATCGGCTGCATCTGACGCTTCAGGAGGCATTC
GGCTGGACGAACAGCCACCTCTTCGAGTTCTTCGCTGGTGAGGCACATTGGGGGATTCCCGACCCCGACAATGATTACGGCCACCAGCCCATGGATGCCA
GCAAAGCGCGGCTTTGCGATATCGTTCGCGAAACCGGCGCCAAGACGATCCACTATCTCTATGACTTCGGCGACAGCTGGGACCATGTGATCAAGCTCGA
AAAATGGTTCGAGAACACCACGACGGAGGGGCTTCCCTTCCTGCTCGAGGCACCCGGTCGTTGTCCTCCGGAAGACGTCGGCGGTGCGCCAGGCTATGCC
GAATACCTCGCCGCCATCAGCGACCCCGGCCATCCCGAGCACGAACACATGCGCCTCTGGGGCCCGGAGCGGTTCGATCCCAACGTCGTCGACCGGAAGG
CGCTCGAAGCCGCCGTCAACGCGTTGTCCGACACATGGATACCGCGGCGACGCGCCACGCGAACAAAATAGGCGTCAACCGCCAATCAAAAGGGCCGCAG
AGCTACGCTTAC

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
372 bp	123 aa	140	511	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MFAGAGRKRWPDDLKAQIAAESLEPGAVVTDVARRHGCRPQQVHDWRRRARLGQLVLPASADTLSFVPVMSESALPAAADPSGSPEAAVVTVEFQGARVE
VRGAPGLAVLSDVFIALRRTRSC

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
320 bp	106 aa	539	858	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MATQPVDFRKGAEGLALLAKETLGHDPMKGVAVVFRAKRADRVKIVVWDGSGLVMYWKRLDGSGFKWPPIVAGVMRMNAAQLSALLAGMDWTRMHAPRIP
QPKALA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1563 bp	520 aa	910	2472	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSDLPDELPSDPAELRVFATALLDRCARLERLLKLAKDAQFGRSSEKLDADQLQLVLEDVEGAVAALETAEDRANPRTSEKRAAERRTNRGQLPEHLPRI
VETLMPAETCCPSCKGDLFEIGRDESQRLDVVPAQYRVIVTRRPKLACRACHGVVLQHAAPERLIRGGLPTERLVAHVIDAKYHWHLPLYRQAQMLATHG
IAIDRSTLAFWVGYAAQELKPLWHRLRQLLLASSKLCVDETPAPVLDPGRGRTKTGYFWALSRDDRPWAGPDPPGVVYAYAPGRGAVHGLRLLEGYRGII
HCDGYQAYKTMTRETRADALSGTLAFCWSHLRRQFVKIEREAAPAPAPVAREALERIAQLYAVEKALRGRSDAERRAGRQAHARPLAAALKQWFEAKLDH
LAQKSDTAKALRYALRHWDGLTLYLDDGRIEMDTNAVERAMRPIKLNAKNSLFAGCDEGAESWAVLASLIETCKLNSVSAEHWLADVLAKLVNGWPATRL
DELLPWASIYTMHAHDRLAA

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
603 bp	200 aa	2469	3071	+	No

ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

MSLNTTAVRIKVTLKDVKPEVMRRLVVPVTLRLDRLHLTLQEAFGWTNSHLFEFFAGEAHWGIPDPDNDYGHQPMDASKARLCDIVRETGAKTIHYLYDF
GDSWDHVIKLEKWFENTTTEGLPFLLEAPGRCPPEDVGGAPGYAEYLAAISDPGHPEHEHMRLWGPERFDPNVVDRKALEAAVNALSDTWIPRRRATRTK

Blast result :

Comments

ISBrsp6 is 61%(orfA), 67%(orfB) and 58%(orfC) aa similar to ISRm14 and 59% aa similar to ISPre3 (orfD).

References

1] Giraud,E., Moulin,L., Vallenet,D., Barbe,V., Cytryn,E., Avarre,J.C., Jaubert,M., Simon,D., Cartieaux,F., Prin,Y., Bena,G., Hannibal,L., Fardoux,J., Kojadinovic,M., Vuillet,L., Lajus,A., Cruveiller,S., Rouy,Z., Mangenot,S., Segurens,B., Dossat,C., Franck,W.L., Chang,W.S., Saunders,E., Bruce,D., Richardson,P., Normand,P., Dreyfus,B., Pignol,D., Stacey,G., Emerich,D., Vermeglio,A., Medigue,C. and Sadowsky,M. (2007) Science 316 (5829), 1307-1312.

2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Saunders,E., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N. and Richardson,P. (2006) Direct Submission GenBank.

3] ISfinder annotation (2008).