ISDds9
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Deinococcus deserti | Deinococcus deserti VCD115 plasmid I |
DNA section
IS Length : 1254 bp
Ends
IR Length : 16/19
IRL : GAGCCGATCCGGAAATTCAGTGACAGTGTGCCGCCCCAAGGGGCGGCACA
IRR : GAGTCCATCCGGATATTCAAGGTTCACACCCGGTAGCTCATTTCGCTAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTCCAAAATAGATG | TA | GATGACACCCTATTC | 2 |
DNA sequence
GAGCCGATCCGGAAATTCAGTGACAGTGTGCCGCCCCAAGGGGCGGCACACTTCAATGATGCGGCTGACTGATCAGCAATGGACCGTTCTGGCGCCACTT
CTTCCAAGGCCCGTCAAGCGCACCAGGCGAGGTCGTACTCGTCAACCTGACCGTGAGGTTCTGGAGGGGATTCTCTGGATCCTTCGTACGGGAGCGCAGT
GGGACGCACTTCCCAAGGGCGAGTATCCGTCCAAAACCACCTGCCATGATCGTTTCCAGGAGTGGAACGAGCAGAACGTCTTCCCGACCGTCCTGTCCGC
GTTGTACGAGTTAGGCGAGGAGGAGCACCTCCTGGACCTCCGGGAAGCCTTCATCGATGGGACCTTTAGCCCTGCCAAAAAAGGGGCGCGGACGTCGGGC
CAACAAAAAAGGGCAAAGGCACCAAGGTGATGATCATGGTGGATGCGAGCGGCGTGCCGCTCGCCGTTCACACCAGCAGCGCCAGTCCAGCTGAGGTGAC
GCTCGTCCACGACACCCTGGAGGCTTCGTTCGGGATGGACTTTCCGAAGCGATTGATTGGGGATAAGGCGTACGACAGTGATGGCCTCGACGTAGAACTG
GAGACACTTGGCATAGAGATGATCGCGCCCAATCGCAGGAATCGAAAGAAGACTCAGGATGGACGTCCTCTGCGCCGCTACCGACGACGGTGGAAGGTGG
AGCGGACGATTGCGTGGTTACAGAGCTTTCGTCGGGTGCGGACGCGGGATGAGGTCAAGGCGCAGAACTTCCTTGGGATGGTTCAGCTGGCCTGCATCGT
CATTCTGCTCCGCCTGGTCACAAGTCCACCTGTACCCTGAGATCTATGGAGTCCGTGGCTATTGGCTTGATTCTGCTCACCCTCCCCCCGCTGCTCTACA
TCGTCAGTCAGAAGACCGGAGTGAGGTACACCTGGACGCCGGATGCCCTCCAGGTGGATACCGGCTTGCGAAGATACATCTTTCCATATGCGACGACGAC
GGCCCGTCTCACCACGCAACCCCTGGGCTCCCGGATCTGGGGCACAGCAGTATCCGGGAGTGTGACGGGCCGTTATACCCTGGACAGCAGCACTGTTCAG
GCCTTAGCTACGACCGCTCGCCCAGCACAGGCCCTGCTTCTAGAGGCTGGTCAGCAAACGTTCTATGTCACGCCAGAGAGGGCTGAGGAGTTCGCGGCGC
GATTTTTAGCGAAATGAGCTACCGGGTGTGAACCTTGAATATCCGGATGGACTC
CTTCCAAGGCCCGTCAAGCGCACCAGGCGAGGTCGTACTCGTCAACCTGACCGTGAGGTTCTGGAGGGGATTCTCTGGATCCTTCGTACGGGAGCGCAGT
GGGACGCACTTCCCAAGGGCGAGTATCCGTCCAAAACCACCTGCCATGATCGTTTCCAGGAGTGGAACGAGCAGAACGTCTTCCCGACCGTCCTGTCCGC
GTTGTACGAGTTAGGCGAGGAGGAGCACCTCCTGGACCTCCGGGAAGCCTTCATCGATGGGACCTTTAGCCCTGCCAAAAAAGGGGCGCGGACGTCGGGC
CAACAAAAAAGGGCAAAGGCACCAAGGTGATGATCATGGTGGATGCGAGCGGCGTGCCGCTCGCCGTTCACACCAGCAGCGCCAGTCCAGCTGAGGTGAC
GCTCGTCCACGACACCCTGGAGGCTTCGTTCGGGATGGACTTTCCGAAGCGATTGATTGGGGATAAGGCGTACGACAGTGATGGCCTCGACGTAGAACTG
GAGACACTTGGCATAGAGATGATCGCGCCCAATCGCAGGAATCGAAAGAAGACTCAGGATGGACGTCCTCTGCGCCGCTACCGACGACGGTGGAAGGTGG
AGCGGACGATTGCGTGGTTACAGAGCTTTCGTCGGGTGCGGACGCGGGATGAGGTCAAGGCGCAGAACTTCCTTGGGATGGTTCAGCTGGCCTGCATCGT
CATTCTGCTCCGCCTGGTCACAAGTCCACCTGTACCCTGAGATCTATGGAGTCCGTGGCTATTGGCTTGATTCTGCTCACCCTCCCCCCGCTGCTCTACA
TCGTCAGTCAGAAGACCGGAGTGAGGTACACCTGGACGCCGGATGCCCTCCAGGTGGATACCGGCTTGCGAAGATACATCTTTCCATATGCGACGACGAC
GGCCCGTCTCACCACGCAACCCCTGGGCTCCCGGATCTGGGGCACAGCAGTATCCGGGAGTGTGACGGGCCGTTATACCCTGGACAGCAGCACTGTTCAG
GCCTTAGCTACGACCGCTCGCCCAGCACAGGCCCTGCTTCTAGAGGCTGGTCAGCAAACGTTCTATGTCACGCCAGAGAGGGCTGAGGAGTTCGCGGCGC
GATTTTTAGCGAAATGAGCTACCGGGTGTGAACCTTGAATATCCGGATGGACTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 375 bp | 124 aa | 56 | 430 | + | No |
Description : First part of the transposase
ORF sequence :
MMRLTDQQWTVLAPLLPRPVKRTRRGRTRQPDREVLEGILWILRTGAQWDALPKGEYPSKTTCHDRFQEWNEQNVFPTVLSALYELGEEEHLLDLREAFI
DGTFSPAKKGARTSGQQKRAKAPR
DGTFSPAKKGARTSGQQKRAKAPR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 471 bp | 156 aa | 370 | 840 | + | No |
Description : Second part of the transposase
ORF sequence :
PCQKRGADVGPTKKGKGTKVMIMVDASGVPLAVHTSSASPAEVTLVHDTLEASFGMDFPKRLIGDKAYDSDGLDVELETLGIEMIAPNRRNRKKTQDGRP
LRRYRRRWKVERTIAWLQSFRRVRTRDEVKAQNFLGMVQLACIVILLRLVTSPPVP
LRRYRRRWKVERTIAWLQSFRRVRTRDEVKAQNFLGMVQLACIVILLRLVTSPPVP
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 785 bp | 261 aa | 56 | 840 | + | Yes |
Chemistry : DDE
ORF sequence :
MMRLTDQQWTVLAPLLPRPVKRTRRGRTRQPDREVLEGILWILRTGAQWDALPKGEYPSKTTCHDRFQEWNEQNVFPTVLSALYELGEEEHLLDLREAFI
DGTFSPAKKGARTSGQQKKGKGTKVMIMVDASGVPLAVHTSSASPAEVTLVHDTLEASFGMDFPKRLIGDKAYDSDGLDVELETLGIEMIAPNRRNRKKT
QDGRPLRRYRRRWKVERTIAWLQSFRRVRTRDEVKAQNFLGMVQLACIVILLRLVTSPPVP
DGTFSPAKKGARTSGQQKKGKGTKVMIMVDASGVPLAVHTSSASPAEVTLVHDTLEASFGMDFPKRLIGDKAYDSDGLDVELETLGIEMIAPNRRNRKKT
QDGRPLRRYRRRWKVERTIAWLQSFRRVRTRDEVKAQNFLGMVQLACIVILLRLVTSPPVP
Blast result :
Comments
ISDds9 is 52% aa similar to ISMma12. The third ORF is putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Siguier, P. (2008) ISfinder direct annotation.