ISDds1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Deinococcus deserti | Deinococcus deserti VCD115 Deinococcus deserti VCD115 Plasmid II Deinococcus deserti VCD115 plasmid I |
DNA section
IS Length : 1248 bp
Ends
IR Length : 28/36
IRL : TGAGGATGACCCGATATGGCGTAGATGGAGATAAGCCCTCACGATAGGAG
IRR : TGGGCCTGGCCCGATATTGCGTAGCTGAAGTTAAGCAGCTGTAGCTTGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGACAGCAGG | CTCATTTCAC | 0 | |
| CGCCCAGGTC | ATG | TTCTGGTGCA | 3 |
| CCCGGCATCG | GTG | AGACCATCTG | 3 |
| CATGAGCGTT | ATG | GACCAGTAGG | 3 |
| ACCTGTCGAA | CAGGCCATCA | 0 |
DNA sequence
TGAGGATGACCCGATATGGCGTAGATGGAGATAAGCCCTCACGATAGGAGGCACCATCATGACTGATCGCAGAATCCACACCGCCGAGTTCAAGCGAGAC
GCAGTGCAGCTTGCTCGAACGAGCGGCAACCTGTCGGGCACCGCGCGTGACCTGGGCATCAACAGCTCCCTGCTGCGCAAATGGATGAATGCTGAGCAGG
AGAAGGGCGAGTTGGCATTCCCTGGTCAGGGCAAACAGCTCCTCACTCCAGAGCAACAGGAGATACAACGGCTTCGCAAGGAGAACGAAATCCTGCGACA
GGAGCGCGAGATCCTAAAAAAGGCGGCAGCCTTCTTCGCCAAAGAAACCACACGCTGAGGTATGAATTCATCCAAGATCAACGCCCCGAGTACCGCCTGG
ACCTGCTGTGCCGGGTGCTGGAGGTCTCGGTGAGCGGGTACCACAGCTGGCGAAGAAGGCCGATCTGCGACCGCAAGGAAGAGGATGCGCTGCTCGAGCA
GCGCATCCAGGAAGTGCATCAACGTAGTAAACGCCGCTATGGGGCGCCACGCATTCACGCGGAGTTGCACGCTGGAGGAGTGCGCGTGTCCCGCAAGCGG
GTGGCGCGTCTGATGCGTGCCAGTGGTCTGCGGGCCAAGGGAAAGCGCCGCTGGGTGCGGACCACGGGGAGCAGTCACACCATGGCCGTCTGCCCGAACC
TGCTTGAGCGGCGGTTCGAGGTCTCGCAGCCGAACCAGGTCTGGGCGTTGGACCTGACGTATCTGCCCACGAAAGAGGGCTGGCTGTATCTCGCAGTCAC
CTTAGACCTGCATTCGCGAGCCGTGGTGGGTTACGCGATGGACATGCAGATGCCAGCCACCTTGCCACTGGCGGCCCTTCAGATGGCTGCTGGCCGACGT
CTTCCGCCACCAGGCCTCCTTCATCACAGCGACAGGGGCAGTCAATACGCGAGTGGCATCTTTCAGGCAGAACTGGCCCGCATGCGGGCCAGGGGCAGTA
TGAGTCGTAAGGGGGATTGTTGGGACAACGCCGTGGTGGAAAGCTTCTTCAGCTCCCTGAAAAGGGAGTTGCTGGAGGACACCATCTTTGAGACCCGGGA
CGTGGCCCGACAAGCCGTATTTGAATTCATCGAGGTCTTCTACAACCGTCAGCGTCGTCACTCGTCTCTTGGGTACTTGACGCCCCTGGAGTTCGAACGC
CAAGCTACAGCTGCTTAACTTCAGCTACGCAATATCGGGCCAGGCCCA
GCAGTGCAGCTTGCTCGAACGAGCGGCAACCTGTCGGGCACCGCGCGTGACCTGGGCATCAACAGCTCCCTGCTGCGCAAATGGATGAATGCTGAGCAGG
AGAAGGGCGAGTTGGCATTCCCTGGTCAGGGCAAACAGCTCCTCACTCCAGAGCAACAGGAGATACAACGGCTTCGCAAGGAGAACGAAATCCTGCGACA
GGAGCGCGAGATCCTAAAAAAGGCGGCAGCCTTCTTCGCCAAAGAAACCACACGCTGAGGTATGAATTCATCCAAGATCAACGCCCCGAGTACCGCCTGG
ACCTGCTGTGCCGGGTGCTGGAGGTCTCGGTGAGCGGGTACCACAGCTGGCGAAGAAGGCCGATCTGCGACCGCAAGGAAGAGGATGCGCTGCTCGAGCA
GCGCATCCAGGAAGTGCATCAACGTAGTAAACGCCGCTATGGGGCGCCACGCATTCACGCGGAGTTGCACGCTGGAGGAGTGCGCGTGTCCCGCAAGCGG
GTGGCGCGTCTGATGCGTGCCAGTGGTCTGCGGGCCAAGGGAAAGCGCCGCTGGGTGCGGACCACGGGGAGCAGTCACACCATGGCCGTCTGCCCGAACC
TGCTTGAGCGGCGGTTCGAGGTCTCGCAGCCGAACCAGGTCTGGGCGTTGGACCTGACGTATCTGCCCACGAAAGAGGGCTGGCTGTATCTCGCAGTCAC
CTTAGACCTGCATTCGCGAGCCGTGGTGGGTTACGCGATGGACATGCAGATGCCAGCCACCTTGCCACTGGCGGCCCTTCAGATGGCTGCTGGCCGACGT
CTTCCGCCACCAGGCCTCCTTCATCACAGCGACAGGGGCAGTCAATACGCGAGTGGCATCTTTCAGGCAGAACTGGCCCGCATGCGGGCCAGGGGCAGTA
TGAGTCGTAAGGGGGATTGTTGGGACAACGCCGTGGTGGAAAGCTTCTTCAGCTCCCTGAAAAGGGAGTTGCTGGAGGACACCATCTTTGAGACCCGGGA
CGTGGCCCGACAAGCCGTATTTGAATTCATCGAGGTCTTCTACAACCGTCAGCGTCGTCACTCGTCTCTTGGGTACTTGACGCCCCTGGAGTTCGAACGC
CAAGCTACAGCTGCTTAACTTCAGCTACGCAATATCGGGCCAGGCCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 300 bp | 99 aa | 59 | 358 | + | No |
Description : First part of the transposase
ORF sequence :
MTDRRIHTAEFKRDAVQLARTSGNLSGTARDLGINSSLLRKWMNAEQEKGELAFPGQGKQLLTPEQQEIQRLRKENEILRQEREILKKAAAFFAKETTR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1023 bp | 340 aa | 196 | 1218 | + | No |
Description : Second part of the transposase
ORF sequence :
AGEGRVGIPWSGQTAPHSRATGDTTASQGERNPATGARDPKKGGSLLRQRNHTLRYEFIQDQRPEYRLDLLCRVLEVSVSGYHSWRRRPICDRKEEDALL
EQRIQEVHQRSKRRYGAPRIHAELHAGGVRVSRKRVARLMRASGLRAKGKRRWVRTTGSSHTMAVCPNLLERRFEVSQPNQVWALDLTYLPTKEGWLYLA
VTLDLHSRAVVGYAMDMQMPATLPLAALQMAAGRRLPPPGLLHHSDRGSQYASGIFQAELARMRARGSMSRKGDCWDNAVVESFFSSLKRELLEDTIFET
RDVARQAVFEFIEVFYNRQRRHSSLGYLTPLEFERQATAA
EQRIQEVHQRSKRRYGAPRIHAELHAGGVRVSRKRVARLMRASGLRAKGKRRWVRTTGSSHTMAVCPNLLERRFEVSQPNQVWALDLTYLPTKEGWLYLA
VTLDLHSRAVVGYAMDMQMPATLPLAALQMAAGRRLPPPGLLHHSDRGSQYASGIFQAELARMRARGSMSRKGDCWDNAVVESFFSSLKRELLEDTIFET
RDVARQAVFEFIEVFYNRQRRHSSLGYLTPLEFERQATAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1160 bp | 386 aa | 59 | 1218 | + | Yes |
Chemistry : DDE
ORF sequence :
MTDRRIHTAEFKRDAVQLARTSGNLSGTARDLGINSSLLRKWMNAEQEKGELAFPGQGKQLLTPEQQEIQRLRKENEILRQEREILKKGGSLLRQRNHTL
RYEFIQDQRPEYRLDLLCRVLEVSVSGYHSWRRRPICDRKEEDALLEQRIQEVHQRSKRRYGAPRIHAELHAGGVRVSRKRVARLMRASGLRAKGKRRWV
RTTGSSHTMAVCPNLLERRFEVSQPNQVWALDLTYLPTKEGWLYLAVTLDLHSRAVVGYAMDMQMPATLPLAALQMAAGRRLPPPGLLHHSDRGSQYASG
IFQAELARMRARGSMSRKGDCWDNAVVESFFSSLKRELLEDTIFETRDVARQAVFEFIEVFYNRQRRHSSLGYLTPLEFERQATAA
RYEFIQDQRPEYRLDLLCRVLEVSVSGYHSWRRRPICDRKEEDALLEQRIQEVHQRSKRRYGAPRIHAELHAGGVRVSRKRVARLMRASGLRAKGKRRWV
RTTGSSHTMAVCPNLLERRFEVSQPNQVWALDLTYLPTKEGWLYLAVTLDLHSRAVVGYAMDMQMPATLPLAALQMAAGRRLPPPGLLHHSDRGSQYASG
IFQAELARMRARGSMSRKGDCWDNAVVESFFSSLKRELLEDTIFETRDVARQAVFEFIEVFYNRQRRHSSLGYLTPLEFERQATAA
Blast result :
Comments
ISDds1 is 67% aa similar to ISStau1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Siguier, P. (2008) ISfinder annotation.