ISfinder Mirror Site

ISCku7

Family IS66
Group ISBst12

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CT573072	ND	Candidatus Kuenenia	Candidatus Kuenenia stuttgartiensis

DNA section

IS Length : 2368 bp

Ends

IR Length : 28

IRL : GTAACACTTTAGTTCTTTGAAAAATTAATAGCGAAAAAAGGTAAAAAAAG
IRR : GTAACACTTTAGTTCTTTGAAAAATTAACAAGCGATATTATAAGTTAAGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACGGCAATCA	AAATAATT	AAGTGAAAGG	8
GATATACTCT	TTTTCTGG	GAAATCCGTA	8
GATTAATAAA	CAATTTTt/g	GCCCTTTATA	10

DNA sequence

GTAACACTTTAGTTCTTTGAAAAATTAATAGCGAAAAAAGGTAAAAAAAGCTGGCGTCTGTTTTTCCTTTGTGGTATAAAAGGCTTCAATAAAGGGAAAC
AGTTTATGAAGCCTATCATATTCCAATACCGTTCACGAAAACTGCATGCGGACGATATCGCCTTTATAAAGGCGCTCATCGAGCAGCATTTTCTCAGAGG
TCGGACTTATATTTCCTGCGAACTATGCAAGCATTGGAACTGGGTGCAGCCCAATGGCAAACATAAAGAATACGCAGCACGAGACCTCCTTTTGAGATTA
GAAGAACAGGGATTGATAACGCTTCCTCCACGTATACGGGCCAAAAACAATCTGAAACCCAAGGTCTTTGTCCAGACACCCCTTTTCGTCAGGAGACCAC
TAGAAGGTAAAATCACAGAGTATGAAAACCTGACAATCCATGTGGTAAAAGACCCTCAGGAAAGATATCTCTGGGAGTATCTCTTCCAACACTATCACTA
CCTCGGCAATCCCCGGCTTGTTGGCGAACACCTCAGACACATCGTACGTATAGGCAATCAGGTTGTTGCCTGCCTGGGGTGGGCAAGCGCCGCATGGAAA
GTCAAAGACCGTGATCGTTTCATTGAATGGGATGAGACGACTAAACGTACACACTTACATTTGATTGCAAATAACGTACGATTTCTCATTCTACCATGGG
TTAAGATCAAACACCTTGCATCCAAGGTATTGTCACTTGCCCTCAGACGTCTGTCAGATGACTGGAATACCGTTTACGGCCATCCCGTTTATTTAGCCGA
AACCTTTGTTGATACCGCGCGGTTTAAGGGTACCTGCTATCAGGCGGCCAATTGGATTCGTGCTGGCAAAACCAAAGGGAGCGCAAAACGGGGCAATCGC
TATCTGTATCATGGCCAACCTAAGGATCTCTACCTCTACCCTCTTCAAAAAAACTTTCGGAGGTTTCTTGACTGTGACCAGGGATGAAGCCATAGCTATT
TTGGAGATGGACAGAGAGGATGCGATTCAAGCCATCCTGATACTGGCGGAGAAAGCAGAAAAATATGACCGGCTTTGCGATAAACCGGGTCCGACTACCC
CTTCCGGTTCGATACCGCCCTATCTAAAGCCCACAAAGAAAAAGAGAAAACGGCCTTGTGGCAGGAAAAAGGGACATAAGGGCGTTTGCCGGAAACGACC
TGAAACAGTAACTGCCTATCAAACGCATTCCATGGAGAGTTGCCCTGATTGCCACCAGCCATTACAAAAACCGGTAAGAACTTATAAGCGATACATCGAA
GATATTCCACGCCTTGACCCTGTTGTGACGGAACATACAGTTCATGGTTACTGGTGTGCTTGTTGCAAAAAGATAGTTCAACCCAAGGTCACAGATGCGT
TGCATGGCGCCCGACTAGGATTGCGCCTTGTTGTCTTTACCGCATGGCTTCACTACTTAATCGGTATCAGTGTAAACAACATCGTAAAGATGCTTTCCGT
ATTTTTCAACCTTCAAATAAGCGCCGGCGGTCTAACCCAGGCATGGAAATCCCTTGCAACACTCCTGGAGCCACAGTATAACGAGATTGGCCAAAAAGTC
TCCGCAAGCGCTGTCTTACATGCAGATGAAACCGGATGGAGGTTGAATGGAAAAACCCATTGGCTATGGTGTTTTACAACACAAAAACTCTGCTACTATC
TCATAACACCAAGTCGGGGGTCTCCTGTCATAAAAAAACTCTTGGGCATTCTATTTGGTGGTATCCTGATCTGTGACTTTCTGGGCGCTTACAACAAGAT
AAGCGCACTGGCAAAGCAGCGGTGTTTCTATCATCTGTTCACGGAACTCGTGAAAGTAGATGCCCACAACCATTCTGCCGCATGGAAAGCTTTCCGGAAA
AAACTCTCCCGGTTGCTCAAGGATGCCATCCGGTTATCAGAGAAAAAAAACCAGATAAGCCTGGTATGCTTTCTTCGGCTAAAAAAAAGATTGTATCGCA
GGCTTGAACAGTTTTTGGCAACGCCTTGTCAGGATAAAGACGTACAAAGATTGACCAAACGCCTGAAGCGCCATAAACAGGAACTCTTTACGTTCCTGGA
ACATGAAGGTGTGAGTCCCTATAATAATCATGCCGAGCAACAGATGCGAAAGCCAGTATTGACCCGAAAGGTATCACAACAGAATCGTTCTGTTCAGGGC
GCCAATACCCAGGCTATCCTTATGACGTTGCTTCGCTCTGCAGAATTGCAAGGAGACAACCCCGTGGAAAACCTTCTGGCATACGCCAAAAAAGCTCTTT
TAACAAAAACCACTTCCGGCTTAACTTATAATATCGCTTGTTAATTTTTCAAAGAACTAAAGTGTTAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
882 bp	293 aa	106	987	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MKPIIFQYRSRKLHADDIAFIKALIEQHFLRGRTYISCELCKHWNWVQPNGKHKEYAARDLLLRLEEQGLITLPPRIRAKNNLKPKVFVQTPLFVRRPLE
GKITEYENLTIHVVKDPQERYLWEYLFQHYHYLGNPRLVGEHLRHIVRIGNQVVACLGWASAAWKVKDRDRFIEWDETTKRTHLHLIANNVRFLILPWVK
IKHLASKVLSLALRRLSDDWNTVYGHPVYLAETFVDTARFKGTCYQAANWIRAGKTKGSAKRGNRYLYHGQPKDLYLYPLQKNFRRFLDCDQG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1371 bp	456 aa	974	2344	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VTRDEAIAILEMDREDAIQAILILAEKAEKYDRLCDKPGPTTPSGSIPPYLKPTKKKRKRPCGRKKGHKGVCRKRPETVTAYQTHSMESCPDCHQPLQKP
VRTYKRYIEDIPRLDPVVTEHTVHGYWCACCKKIVQPKVTDALHGARLGLRLVVFTAWLHYLIGISVNNIVKMLSVFFNLQISAGGLTQAWKSLATLLEP
QYNEIGQKVSASAVLHADETGWRLNGKTHWLWCFTTQKLCYYLITPSRGSPVIKKLLGILFGGILICDFLGAYNKISALAKQRCFYHLFTELVKVDAHNH
SAAWKAFRKKLSRLLKDAIRLSEKKNQISLVCFLRLKKRLYRRLEQFLATPCQDKDVQRLTKRLKRHKQELFTFLEHEGVSPYNNHAEQQMRKPVLTRKV
SQQNRSVQGANTQAILMTLLRSAELQGDNPVENLLAYAKKALLTKTTSGLTYNIAC

Blast result :

Comments

ISCku7 is 46% aa similar to ISArch7.
The first ORF is a passenger gene annotated as unknown protein.

References

1] Strous,M., Pelletier,E., Mangenot,S., Rattei,T., Lehner,A., Taylor,M.W., Horn,M., Daims,H., Bartol-Mavel,D., Wincker,P., Barbe,V., Fonknechten,N., Vallenet,D., Segurens,B., Schenowitz-Truong,C., Medigue,C., Collingro,A., Snel,B., Dutilh,B.E., Op den Camp,H.J., van der Drift,C., Cirpus,I., van de Pas-Schoonen,K.T., Harhangi,H.R., van Niftrik,L., Schmid,M., Keltjens,J., van de Vossenberg,J., Kartal,B., Meier,H., Frishman,D., Huynen,M.A., Mewes,H.W., Weissenbach,J., Jetten,M.S., Wagner,M. and Le Paslier,D. (2006) Nature 440 (7085), 790-794.

2] ISfinder annotation (2008).