ISNpu9
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAAY02000118 | ND | Nostoc punctiforme | Nostoc punctiforme PCC 73102 |
DNA section
IS Length : 778 bp
Ends
IR Length : 24/28
IRL : GGCGTTGCATAATAGAGGGATGAATTGAGGCTATCTACTGTGCAATGTCC
IRR : GGCGTTGCTGAATGAAGGGATGAATTGATTTATAAAAGTATGGTTTGATA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCAAAATAACCTAAGAA | CTAAAATT | TTGTACTTAGTCGTAA | 8 |
DNA sequence
GGCGTTGCATAATAGAGGGATGAATTGAGGCTATCTACTGTGCAATGTCCATACTGCGGAGCTACGGAAATTCGTAAAAACGGAAAGCGAAGAGGTAAAC
AGAATCACATTTGTACTAAATGCGAGCGCCAATTTATTGACGTATATGATCCACCAAAAGGGTATTCCGAAGAGCTAAAGCAAGAATGCTTGGAAATGTA
CCTAAATGGCATGGGTTTTCGCCCAATTGAACGAGTCAAAGGTGTACATCATACAACTATTATTTTCTGGGTTAAACAAATGGGCGAGAAACTGCCAGAT
GTCCCAGAAGAAAATGTGATTCCAGAAGTGGGAGAACTAGATGAATTAGAGACATTTGTTGGGTCAAAAAAAACAAAATTTGGCTGTGGACAGCAGTAAA
CCACTTTACTCAAGGTATTTTAGCTTGGGTTTTAGGAGACCATAGTCTTGTACTGAGCGAAGTCGAAGTAGCTGAAACTTTTAAGCCACTTTGGGAAAAT
ATTGAAAAGTGGAAATGCTATTTCTATGTAACTGATGGCTGGAAGGTTTACCCCAGTTTTATTCCCGATGGAGACCAAATTGTGAGTAAAACATATATGA
CGCGGGTAGAAAATGAAAATACAAGGCTTAGACATTATCTTGCACGCCTTCATCGCAAAACTTTATGTTATTCCAAATCAGAACAAATCCTGAGATACTC
AATTAAATTATTACTCCACTACTTGAAGTATCAAACCATACTTTTATAAATCAATTCATCCCTTCATTCAGCAACGCC
AGAATCACATTTGTACTAAATGCGAGCGCCAATTTATTGACGTATATGATCCACCAAAAGGGTATTCCGAAGAGCTAAAGCAAGAATGCTTGGAAATGTA
CCTAAATGGCATGGGTTTTCGCCCAATTGAACGAGTCAAAGGTGTACATCATACAACTATTATTTTCTGGGTTAAACAAATGGGCGAGAAACTGCCAGAT
GTCCCAGAAGAAAATGTGATTCCAGAAGTGGGAGAACTAGATGAATTAGAGACATTTGTTGGGTCAAAAAAAACAAAATTTGGCTGTGGACAGCAGTAAA
CCACTTTACTCAAGGTATTTTAGCTTGGGTTTTAGGAGACCATAGTCTTGTACTGAGCGAAGTCGAAGTAGCTGAAACTTTTAAGCCACTTTGGGAAAAT
ATTGAAAAGTGGAAATGCTATTTCTATGTAACTGATGGCTGGAAGGTTTACCCCAGTTTTATTCCCGATGGAGACCAAATTGTGAGTAAAACATATATGA
CGCGGGTAGAAAATGAAAATACAAGGCTTAGACATTATCTTGCACGCCTTCATCGCAAAACTTTATGTTATTCCAAATCAGAACAAATCCTGAGATACTC
AATTAAATTATTACTCCACTACTTGAAGTATCAAACCATACTTTTATAAATCAATTCATCCCTTCATTCAGCAACGCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 360 bp | 119 aa | 40 | 399 | + | No |
Description : First part of the transposase
ORF sequence :
VQCPYCGATEIRKNGKRRGKQNHICTKCERQFIDVYDPPKGYSEELKQECLEMYLNGMGFRPIERVKGVHHTTIIFWVKQMGEKLPDVPEENVIPEVGEL
DELETFVGSKKTKFGCGQQ
DELETFVGSKKTKFGCGQQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 405 bp | 134 aa | 345 | 749 | + | No |
Description : Second part of the transposase
ORF sequence :
IRDICWVKKNKIWLWTAVNHFTQGILAWVLGDHSLVLSEVEVAETFKPLWENIEKWKCYFYVTDGWKVYPSFIPDGDQIVSKTYMTRVENENTRLRHYLA
RLHRKTLCYSKSEQILRYSIKLLLHYLKYQTILL
RLHRKTLCYSKSEQILRYSIKLLLHYLKYQTILL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 710 bp | 236 aa | 40 | 749 | + | Yes |
Chemistry : DDE
ORF sequence :
VQCPYCGATEIRKNGKRRGKQNHICTKCERQFIDVYDPPKGYSEELKQECLEMYLNGMGFRPIERVKGVHHTTIIFWVKQMGEKLPDVPEENVIPEVGEL
DELETFVGSKKNKIWLWTAVNHFTQGILAWVLGDHSLVLSEVEVAETFKPLWENIEKWKCYFYVTDGWKVYPSFIPDGDQIVSKTYMTRVENENTRLRHY
LARLHRKTLCYSKSEQILRYSIKLLLHYLKYQTILL
DELETFVGSKKNKIWLWTAVNHFTQGILAWVLGDHSLVLSEVEVAETFKPLWENIEKWKCYFYVTDGWKVYPSFIPDGDQIVSKTYMTRVENENTRLRHY
LARLHRKTLCYSKSEQILRYSIKLLLHYLKYQTILL
Blast result :
Comments
ISNpu9 is 84% aa similar to ISMae3.
The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] NCBI Microbial Genomes Annotation Project. (2004)