ISfinder Mirror Site

ISRosp3

Family IS1595
Group ISNha5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAYB01000002	ND	Roseobacter sp.	Roseobacter sp. CCS2

DNA section

IS Length : 3472 bp

Ends

IR Length : 25

IRL : CGGCCTTATGTATCAAACGCACCAAGCAGGGTGTGAGGGATGATTAGGAC
IRR : CGGCCTTATGTATCAAACGCACCAACAACATACCTTATAAAAACAAGGCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CAGTGAATGTAC	AGTTTGTC	CCGCTTAGCTGTCGT	8

DNA sequence

CGGCCTTATGTATCAAACGCACCAAGCAGGGTGTGAGGGATGATTAGGACTCCACAACCGCATAGTCCCGTGCAACTTTGGAAGCCATGTCATATCTCCG
CATCATTGGTGTATTCCTATTTCTTGCCCCGTTCACCGCAATGTTCGGTGAGCCGTTCTATGAAGCCTTATTTGATCGCTTGGGGTGGGATACGGAAACA
TTGGCGGGGCCAGTTATGAACTGGGTGTCGGAAAATACTGCCGTGTTAACCTTGGCTATGGGCCTTGGTGCCGGAATATGGATTCACTATTGGGGGCAAA
AAGTTTCGGCTCGAATGGGCGATTCAGAAAAAGAAATTGATTGGGCATTCTTGGAGAGCGAGATACACCGCATCAAGATTTTGATAGCTGGCGATATTGC
CCGAAAGGATGAAGTTGAAGATGGCTTCTCTACAATCGGGCAAGAGTCCTTCTCAGGATTTTACGCGCTTTTAATGTATCTTTCGGAAAGAAATATGGCA
CCAGAAGATGGCATACCTAGCCCGCAGATAATGGTAGACGAAGACCTATTCTTCGAAATCTCGACCCGGTACATAGCTGCTGTTGAACCGTACATTCGTG
CCAAGAACCCGCGTGGGGTAAGGGACCTCGGCGGGTCAATCATTAACTTGGTATATCAATGGGAGCGTGAAAAGCGGATTGAGGAATCTAAAGTCTCACC
TCAGTAGCTTCAATCGCAGACATGCACTGCAACATGTATGCCGCACTGAATTTTCCACGACTCAATTTGTTGCGAACGTTGGGTTCTTTGTCACCGATTA
ACTCTGCAAGTTGTGCATAGCTAACGCCTTTCAACTTTAGTTCGGCCTTTAGGATGTTGGCCGCTTTGGTTTCCCATTCGTTCTGCTCAGGCATTTGTCA
CTCCAGAAATGATAAATGCACTATATTCGTGATATTTTATATTGTAAACCGTACCTGTATGTACTATATATGTGATATAAGTAATCACGGTAGATACAAT
GGCACAGCACTTTCTTCTCTCAGCGGCATCACGCACACTTAGCCTTCGCAGCATCTACAAAGCTGGCGAAGATGCAGCGCATGCGACGTTCTGCGAAATG
CGGTGGCCTGAGACAGATGGCGAAGCTGTTTGCCCACAGTGCGGCCATGACGAGACATACAACATCACTACGCGCCGCAAGTTTAAGTGCAAGGCATGTG
CGCACCAGTTTAGCGTCACGTCCGGCACAATCTTTGCGTCGCGCAAGATGGACTTTGTAGACCTGCTGGCCGCGATTTTCCTGATCGTGAACGCATCCAA
GGGCATGAGCATGGTGCAGTTGTCGCGTGATCTGGATTGCCAATACAAAACGGCTTTCGTTCTGGCGCACAAACTACGCGAAGCTATGGCAACTGAGGTT
CACACTGGCGAAGTGCTGGACGGTCATGTTGAGATTGATGGCGCATACTTCGGCGGTCACATCCGCCCCGCCAACGCCAAGGCTGACCGCGTAGACCGTC
GCCTTAAGCGCAATCAGACAGGCAAGCGCCGCGTTGTGGTTGCAATGCGTGAGCGTCAGGGCCGCACACTACCATTCGTCGCTATGGGCGAGGGTGAAGG
CGTTGCACTGGCTGTAGAGAACGTCAGCCGCACAGCCACTATGTCAGCCGATGAAGCGTCCCATTGGGATACGCTGCATGATGGCTGGATGGTTAATCGT
GTGAACCACAGCGAGATTTACAGCGACCACGGCAAGCACACCAACATGGTGGAAAGCTTCTTTAGCCGCCTGCGCAACATGATCGAAGGCCAGCACCACG
GCGTTAGCCCCAAGTACCTGCACCAGTATGCAAACCACTCCGCATGGCTGGAAGACAACCGCCGCACTGACAACGGCACATTGGCACATATCGTTGCGTC
TGACGCGATGGGCGCGCCTGTAAGCCGCAACTGGAAAGGTTATTGGCAGCGGGCGGCTTAATTGCAGAAAGTCAAGAATGCGTCAAAATTAAGGCACGCA
TGCGGAAGAACAAAGAAGGACTCGCGTTATGGGCAGAATTCCGCTAGTAGTTGATACGTGAGTGGCCGGATGGGTTCTAGTAAAACCCACCCCGGCTTTT
TCTTAAATCGCGCTGCTACGCGACCACCACTCACGGTACTTGTTGATAGCCCTTAGAATCAAGGGCCGTCAAGGCTGTATTGCAGCGCACAGGGTCCGGA
AAGGACGTATCGATGCAGTACGCAATGAAGCTTATTGAACATGAGGTGCAGGGTTCGCCTGTTCAGCAACGGGCTACAGATGGCTATATCAATGCCACTG
CAATGTGCCGCATTGCTGGTAAAATGTGGGGTCACTATCGCGAAAATGCGGGAAACAAGGCGTTCCTGTTGGCTTTAGAGAGCGATATCGGAATTCCGAT
ATCGGAATTGGTGAAGTCTGTAAAAGGTGGCGACCCGCGCGTTCAGGGGACTTGGGTACATCCGCAAGTGGCCATACACTTGGCTCAATGGCTATCACCT
GAATTTGCCGTAAAGGTGAGCAAGTGGGTGTACGATTGGCTGAGTGGGCAAGGTACGCAAAGCGGGTACAGCTATCACCTAAAGCGCTACACAACCAACA
TGCAAAACGTACCATATGGCCACTGGTCAATGCTTCAGGAGATGATGATTGGGCTAATCGGGCCAATGGAGTCTCGCGGCTATGTCTTGCCGGAAAATCT
GTTACCTGACATTTCGGAAGGTAAGATGTTCTGCAAGTTTTTGCGTGATGACCTACACGTAGACACGGACTCATTGCCAACGTACCGCCATGACTTTGAA
GATGGGCGGGTAGTATACCCGAAAGCTTATCCTAATGATCTACTTCCGGCGTTTAGGGCGCATTTCTTTGAGGTTTGGCTCCCCAGCAAGGCGCTCAACT
ACTTTCAAGGGAAAGACCCAAAGGCGCTTGAGTATCTACCTTACTTGCTGCCAAAAAAAGACGCTGCCGAATAGGATGGTGCTGGCCCATCATTGGGTCA
GCCTCCACAGACACCCATCGGGCCCAAAATCCTGCACCACGTGCCCGTCTTTCTGCTGGCGTTGCAGCGCATCACGTACCCGCTTTAGAACGTCAGCCCG
CAATGCATTTTCAGCATCCCAGCCCTTACAATCGATTATAGCGGCAGCGATGGCTTCATCATCCGCCGCACCTTGCCGAAGCCTGTCGCGTGTCATTAGC
TTCAACTCATTCCGCCCGAATAGCTGTTTGTACTTCCCACGCGGCGCTATCCCCTTAGGGTCGTTCTGATAGCCACACAGCACCAGAGCGCGGTCTATGG
CGTCCAGATCGGCTTTGAGAGCGTCAGCGGCCTTTAGCGCAACCTTGTGCTGGCCCGCTATCTCGCCGCGTTTTTTGATTAGTCCGTGTATGGTGTTCTG
CATATCCGCATTATGGATTCACAGCCTTGTTTTTATAAGGTATGTTGTTGGTGCGTTTGATACATAAGGCCG

Protein section

ORF number : 5

ORF 1

Length		Begin	End	Strand	Fusion ORF
621 bp	206 aa	87	707	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MSYLRIIGVFLFLAPFTAMFGEPFYEALFDRLGWDTETLAGPVMNWVSENTAVLTLAMGLGAGIWIHYWGQKVSARMGDSEKEIDWAFLESEIHRIKILI
AGDIARKDEVEDGFSTIGQESFSGFYALLMYLSERNMAPEDGIPSPQIMVDEDLFFEISTRYIAAVEPYIRAKNPRGVRDLGGSIINLVYQWEREKRIEE
SKVSPQ

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
207 bp	68 aa	894	688	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MPEQNEWETKAANILKAELKLKGVSYAQLAELIGDKEPNVRNKLSRGKFSAAYMLQCMSAIEATEVRL

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
963 bp	320 aa	999	1961	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAQHFLLSAASRTLSLRSIYKAGEDAAHATFCEMRWPETDGEAVCPQCGHDETYNITTRRKFKCKACAHQFSVTSGTIFASRKMDFVDLLAAIFLIVNAS
KGMSMVQLSRDLDCQYKTAFVLAHKLREAMATEVHTGEVLDGHVEIDGAYFGGHIRPANAKADRVDRRLKRNQTGKRRVVVAMRERQGRTLPFVAMGEGE
GVALAVENVSRTATMSADEASHWDTLHDGWMVNRVNHSEIYSDHGKHTNMVESFFSRLRNMIEGQHHGVSPKYLHQYANHSAWLEDNRRTDNGTLAHIVA
SDAMGAPVSRNWKGYWQRAA

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
762 bp	253 aa	2213	2974	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MQYAMKLIEHEVQGSPVQQRATDGYINATAMCRIAGKMWGHYRENAGNKAFLLALESDIGIPISELVKSVKGGDPRVQGTWVHPQVAIHLAQWLSPEFAV
KVSKWVYDWLSGQGTQSGYSYHLKRYTTNMQNVPYGHWSMLQEMMIGLIGPMESRGYVLPENLLPDISEGKMFCKFLRDDLHVDTDSLPTYRHDFEDGRV
VYPKAYPNDLLPAFRAHFFEVWLPSKALNYFQGKDPKALEYLPYLLPKKDAAE

Blast result :

ORF 5

Length		Begin	End	Strand	Fusion ORF
414 bp	137 aa	3403	2990	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MQNTIHGLIKKRGEIAGQHKVALKAADALKADLDAIDRALVLCGYQNDPKGIAPRGKYKQLFGRNELKLMTRDRLRQGAADDEAIAAAIIDCKGWDAENA
LRADVLKRVRDALQRQQKDGHVVQDFGPDGCLWRLTQ

Blast result :

Comments

ISRosp3 is 89% aa similar to ISRssp2.
The third ORF is the transposase, others are passengers genes.

References

1] Mary Ann,M.A., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.