ISNwi4

  • Family IS1595
  • Group ISNha5
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007406 ND Nitrobacter winogradskyi
Nitrobacter winogradskyi Nb-255
DNA section
IS Length : 3671 bp

Ends


IR Length : 23

IRL : CGGTATTAGGTAGCAAACTCACCTTGGTATTTATATCAGCAACACTGACA
IRR : CGGTATTAGGTAGCAAACTCACCAAGCAACGGATGGCGGTTTTCCTGTGT

Insertion site


Left flankDirect repeatRight flankDR Length
GGATCGTCGAACCACTTCCGCACAGCGGTCC8

DNA sequence

CGGTATTAGGTAGCAAACTCACCTTGGTATTTATATCAGCAACACTGACATGTCGAGTCCACAAGGCTTATTAACTAAATCTTAACCAAAGATTCTAGGA
ACAAATGTGGGCCACGGCTTGGTTGGGGTTGATTTCAGTCCAGTTCCAGACTACAAGACTCAGCTATGGAGACCTTGCGAGAATTTATCACCCGGCGTCA
TAAAGAATTGGACGAGCTAGAAGCTCCGCTCCGGCAGCAACTAGCCGCCATAGCCGACGAGCGCGAAGAGCTTCGTCGTGCTGCACTTGCAGCCGGTCCG
CCGCCCATGCGTCCGGCTAATACCGTAACTCAGCAAGCGGACGAAGGCCGCCGATCACGCCGCACGATTCCTGAAAAGACAATCAAAGACGCCGTTGTCG
AGGTTCTAAGAGCCAAGGGCTCCGGGATGACTGCGCTAGAAATCTTGGCTGCCATTAATCTCAAGTTCGGGACGGAATATCCGAGAACCAGCCTAAGCCC
GCAGCTTTCACGCCTGAAAGCAGACGGGAAAATCAATCGGGATGGCATCGTATGGACGCTTGAAAAGAAGCTTATCGACTACGTCGATCAGCCGTTCCAA
CGGGATATCGAGGAGGCTTTTAAATGAAAACCGGAGCCTTTCGGCCCCGGTTCAAAGAGCTTGGGGGTGCATAGTGCAAGGGCGAGTTGTCCAGCTCGGG
CCCCGGCGGGTGCAAGTCCCGCTATCTCCACCAAGTAGCCCCTTCCACAAGGGCTGCGAGTCAATCCCTTCCAAAAGGATTGGCCTATCGTCTACTGGCT
CCCCTCCAAAAGGAGCCACCCTTAACTTTCTTATAGAATCACACCTGACGGGCTACTACAAGGCCTTGCTCTTTTTCAGTCTCTACAAATGAAATACCAC
CGGTTTCCAAAGCAGCTTTCATTGCCGTTAGATTGTTTCGGGTTGGCTCCCGCCGCCCCGCCTCAAAGTCTCTTACGGTTGAGACGCCGACGTTCGCGGC
TTTTGCAAGCGCGTCTTGAGGCCAATTTAGCCATGCTCGGGCGGCTCGACACTGTTCCGGGGTCATTGCCGGAATCTAGTGACCCTAACTATTTCCGTCA
AGGTCATAGATTTTCGTTGACGCCATCACTTTCATTACTTATCAATGAGGTCAACGGAAAACGTTGGTCTCATGTCGCAACACTTCCTCCTCTCAGCAAA
GGCGCGGACGCTCAGTCTGGCGAAGGTCGCGCGCCTTTCTGACGACGAGGCTTATGATGCGTTCCGGCTGATCCGTTGGGCCGCGACCGATGGTGCGCCG
GTTTGTCCGCGTTGCGAATGTGCTGCCGTCTACACCTACACGACCCGCCGCCTATTCAAGTGCAAGGCTTGCTCGCATCAGTTCTCAGTTACGTCGGACA
CGATCTTCGCAAGTCGTAAGCTCCCGGTGCGCGACTATCTCTTGGCTATTGCGATCTTCGTCAACGGCGCAAAAGGCCATAGCGCGCTTCAACTGAGCCG
CGATCTCGATTGCCAGTATAAGACTGCTTTCGTGATGGCTCACAAAATCCGCGAGGCGTTGGCTTCGGAAGCTCATGGCGCAATCGCCTCTGGCGAGGTC
GAAGTGGATGGCGCATACTTCGGCGGCTACGTGAAGCCTGCAAACCACAAAGGTAACCGCCGCGACCGCCGCCGCATCATCAATCAGAACGGCAAGCGAC
GCGTGGTTGTCATCATGCGTGAGCGAGGCGGGCGCACGCTGCCTTTCGTTTTTAAGAGCGAAGATGCTTCGCTTGTGAAGATCGCCCAAACCGTTGCACC
CGGCAGCGTCATTCACGCGGATGAAGCGACGCATTGGGACGCGCTACATGCGCGCTTCCTGACCAAGCGGATCAATCACAGCGAAAGTTATTCGGACGGC
GATGCTTGCACCAATCAAGCGGAGTCGTTTTTCTCTCGGATGCGCCGCGCCGAAATCGGCGTTCATCATCGTATCGCTGGCGACTATCTATCAGCCTATG
CTGGTGAAATGGCTTGGCGCGAAAACAATCGCCGTATCAGCAACGGCGAACAATATCTGATGGTGGCGGACGCCGCTTTGCAACATCCGGTATCGCGTCA
GTGGAAAGGTTATTGGCAACGAAACTCTTGACTCGGTCGTGAGTTAACGCTTGAACCACTTCGAATCCGCTTGGGACAAGCGCCCCTAAGCAGATTCTTG
GTAGCTGTCAAGCCTTTTTGATTGAGTGTTGCCAAAATCCCTCATATAATGCGATCTCGAATCGCCGATGAGGGCATCTGCTCCACCGGAGCGGTCGCGT
GCGATTCAGGAGAATACCTATGCCGGTCAAACAAGGCCTTGGCCTTCCAATCAGACACATCCCCATTTCAGGCCCGGCGGTCGATTTGCTCGATTCCCGC
AAAGAGCATAACGACAATAGTCCTGTGACCGCGCAAGAGTTGCGCGTTAGCTTCGAAAAGGTCAAAACGAGCCTGTTTTCGGGGTTAGACACGCTGTTGC
AGTCCAAAATCTAAGGACTCAACATACCTAACCCTCAAGGAACGGGGGCCTTACAATTGAGCGAGCCTGACTTCTCAGAAACCAAAATTTCTGAGCGCGT
AGAATTTTTGCTCAATGTTGCGCAAGAACTGATTGAACGCGGTATCCTGCCTGACGAAAAACTAATTCTGCACGAGGGCAGAGTTCGCCTTGCTGTTGAA
TCATATTTTCTCCTCAACAAGGCCTATAAAAAGTGGCGTATTAATTCTGGGCATCACACCGAGAAACCTAAAATTGCTGCTCTACAAGCAACTACGATCG
CCCGCATTCAGCCCTTTATCCCAATTAACCACGCCAACGCAGAAACGTTAGCGGAAGCGCGCTGCAATGAGATTTTCGCTCTAACTTATGCGTTCGCCAT
TCTTGAAAAAGACTTCAATCCCAGTACGCCTCCCAAGAAGGATCTGACGCTGAGGCTGTTAGATATCATCAGCGCGTCTGAGGTTGAGGCGATTGAGCCC
TACATCCAAGACACCAACCTAGAAATCAAAAGAAAGTTGGACGAATACACATTGGCGATTCCGCCATCTGACAAGCATAAGATCGATAGTCTAATCACAA
TCTTTGAGCTAATTTCAGCAAAGGGCGACGCCCTTAAGTAAGCTATCGTCTAATCTCCCAAAGCCGCTGCCGCCCCTCACCAGTTTTGTGAGTGAGGGCC
CCTTGTTCTGTCAGACCTCTGAGGGCCTTTGAGACTCTTTTGGTTACGTCAGTTAGCGCACGTCTGTCTCGCGCATCCTGACCGTTCAAAGCCACGATAG
CCTGAGCTATCTCGCGGCTTCCAAGCGGTCGCGGCGCGTCTCTCAATTCATCCAAAATCGCCCGCGTTAGCTCGCCCCGTCCGAACAGGACCTGCCGTTT
CTGGCGCGGCATTTCCGCATTCAGATCGCCGGTATAGCCAAGCGTTCCTAGCACCCGATCCAGCGCGCCAATGTCGTTCTTAATCTCGGCCATCCGGTCG
CGGATACGCTCGGCTTCGTTGAATAGATCGGCGCGCTTTTTCAGCAAGCCGGAAATGGTGTGTTCGTAGGTGTCGGTTCGGGCAAGGCGGATGGTGTCTG
ACATCCCGCATTGTGTGACTCACACAGGAAAACCGCCATCCGTTGCTTGGTGAGTTTGCTACCTAATACCG
Protein section
ORF number : 4

 

ORF 2
LengthBeginEndStrandFusion ORF
228 bp75 aa8391066-No
ORF function : Passenger Gene
Annotation : Transcriptional regulator, XRE familyDescription : Transcriptional Regulator factor

ORF sequence :

MTPEQCRAARAWLNWPQDALAKAANVGVSTPTRNNLTAMKAALETGGISFVRDFEAGRREVETEKEQGLVVARQV

 

Blast result :
ORF 1
LengthBeginEndStrandFusion ORF
462 bp153 aa166627+No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

METLREFITRRHKELDELEAPLRQQLAAIADEREELRRAALAAGPPPMRPANTVTQQADEGRRSRRTIPEKTIKDAVVEVLRAKGSGMTALEILAAINLK
FGTEYPRTSLSPQLSRLKADGKINRDGIVWTLEKKLIDYVDQPFQRDIEEAFK

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
987 bp328 aa11452131+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRSTENVGLMSQHFLLSAKARTLSLAKVARLSDDEAYDAFRLIRWAATDGAPVCPRCECAAVYTYTTRRLFKCKACSHQFSVTSDTIFASRKLPVRDYLL
AIAIFVNGAKGHSALQLSRDLDCQYKTAFVMAHKIREALASEAHGAIASGEVEVDGAYFGGYVKPANHKGNRRDRRRIINQNGKRRVVVIMRERGGRTLP
FVFKSEDASLVKIAQTVAPGSVIHADEATHWDALHARFLTKRINHSESYSDGDACTNQAESFFSRMRRAEIGVHHRIAGDYLSAYAGEMAWRENNRRISN
GEQYLMVADAALQHPVSRQWKGYWQRNS

 

Blast result :
ORF 4
LengthBeginEndStrandFusion ORF
462 bp153 aa31433604-No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MSDTIRLARTDTYEHTISGLLKKRADLFNEAERIRDRMAEIKNDIGALDRVLGTLGYTGDLNAEMPRQKRQVLFGRGELTRAILDELRDAPRPLGSREIA
QAIVALNGQDARDRRALTDVTKRVSKALRGLTEQGALTHKTGEGRQRLWEIRR

 

Blast result :
Comments
The third ORF is the transposase, others are passengers genes.
ISNwi4 is 86% aa similar to ISAzca1.
References
1] Starkenburg,S.R., Chain,P.S., Sayavedra-Soto,L.A., Hauser,L., Land,M.L., Larimer,F.W., Malfatti,S.A., Klotz,M.G., Bottomley,P.J., Arp,D.J. and Hickey,W.J.(2006) Appl. Environ. Microbiol. 72 (3), 2050-2063