ISGst1
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010420 | ND | Geobacillus stearothermophilus | Geobacillus stearothermophilus plasmid pGS18 |
DNA section
IS Length : 1613 bp
Ends
IR Length : 18/26
IRL : GTAACTATTCAGCCACATCATAAAGTGAGCTGAATATTTTCTTCTTTCCT
IRR : GTAACTATTCACCCCAGTGCTAGTGTATAAAAAAGCCCAGCACAAATAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTATATACAA | AAAAGAAC | CTTAGTCTGA | 8 |
DNA sequence
GTAACTATTCAGCCACATCATAAAGTGAGCTGAATATTTTCTTCTTTCCTTGTCTATGATGTGAATACTGATAGACAAGGAGGTGAATCGCATGTTGACG
GTACAACAAGCTGTATTTACAGTTGAAAGCTTAATCAGCAAAGTCCAACAACAAAAGCAGCTCATTACTCACCAACAACAAGTCATTGAGCAACTGTTGA
AAGAAAACAAACAGCTACGCAAAGAAAATGAACAACTGAAGTACCGTGTTCAAGAGCTGGAAGCACGCACGAAAAAAAACAGCTCTAATAGCCATTTGCC
CCCATCTTCTGACCGTTTTGAGAAAAAGCGTTCCTCCCGCGAGCCGTCTGGCAAAAAGCCTGGTGGGCAAGAGGGACATGAGGGGAAGACGCTCCGTCAA
GTGGAACATCCACATCATCGTGTCGTCCACCGTGTGCATACGTGTCAAGGATGTGGAGCTTCTTTGCGTGAAGTCAAACCGTTCAAAGTCGATATCCGTC
AAGTGTTTGATGTCCCTCCTGCGGCGATCGAGGTGACACAACATGAACGTGAAGTGAAATCGTGTCCACATTGTCGATGCGTGCAACAAGCCGAATTCCC
ATCCCATGTCACGAATCATGTGCAATACGGTCCACGGCTCACGGCGCTCGTTGTTTATTTACATCATATCCAATTGATCCCGTACAAGCGTTTAAGTGAT
ACAATCGAAGCGTTATATCAACACTCGATTAGTACGGGAACTCTTGCCAATATGGTGAAACGAGGACGTGAATCGTTGGAATCAAATATGGACATCATCG
AAGACGCCTTACTGGAATCCAACATCCTGCATGTCGATGAAACGAGTTTGCGCATCAATGGGAAACTCGCATGGGTGCATGTCGCGTGTACATCGAGATA
TACATACTTGGCTCCTCACGCTTCTCGTGGAAAAAAAGCGACCGATGATATCGGGATTCTTCCCCGATATGAAGGGACGATGATGCACGATGCGTTCGGT
ACGTATCCGAAATACACACATGCCACCCATGCCCTTTGTCATGCCCACCATTTGCGTGAGTTAAAAGGATTCATCGAACAAGGGCATACGTGGGCGATGC
GCATGACCACGTTTCTGTTAGCCGCCAAGCAAGCCGTCGAAGCCCATCACGGTGCACTTTCCGAAGAAGAAGCGAGACGGTGGGAACGAGTGTATGATCG
CATCCTAGAAAGAGCACAACACCGATTAGAAACGATGACGCCTCTTCCGAAAAAAGCACTCGCTTTTGTTCGACGCCTTCAAAAACGAAAGGAAGAAGCG
CTGCGTTTCTTACGTGAAGTACATGTTCCCTTTGATAACAACCAAGCCGAACGCGATCTTCGCATGGTCAAAGTCAAAGAGAACATTTCGGGTACGTTTC
GCGAAGAAACATTCGCGCAGTCGTTTTGCATCGCAAGAAGCATCGTTTCCACACTGACGAAACACGAAAAAAACGTGTGGGATTCGTTATGTCTTCTGTT
GGCAGGCGAAACGATCGATCGAGTTCTTTCCGCTACCTAGGGCATTTTCTATGACCGAAATGCCCTATTTGTGCTGGGCTTTTTTATACACTAGCACTGG
GGTGAATAGTTAC
GTACAACAAGCTGTATTTACAGTTGAAAGCTTAATCAGCAAAGTCCAACAACAAAAGCAGCTCATTACTCACCAACAACAAGTCATTGAGCAACTGTTGA
AAGAAAACAAACAGCTACGCAAAGAAAATGAACAACTGAAGTACCGTGTTCAAGAGCTGGAAGCACGCACGAAAAAAAACAGCTCTAATAGCCATTTGCC
CCCATCTTCTGACCGTTTTGAGAAAAAGCGTTCCTCCCGCGAGCCGTCTGGCAAAAAGCCTGGTGGGCAAGAGGGACATGAGGGGAAGACGCTCCGTCAA
GTGGAACATCCACATCATCGTGTCGTCCACCGTGTGCATACGTGTCAAGGATGTGGAGCTTCTTTGCGTGAAGTCAAACCGTTCAAAGTCGATATCCGTC
AAGTGTTTGATGTCCCTCCTGCGGCGATCGAGGTGACACAACATGAACGTGAAGTGAAATCGTGTCCACATTGTCGATGCGTGCAACAAGCCGAATTCCC
ATCCCATGTCACGAATCATGTGCAATACGGTCCACGGCTCACGGCGCTCGTTGTTTATTTACATCATATCCAATTGATCCCGTACAAGCGTTTAAGTGAT
ACAATCGAAGCGTTATATCAACACTCGATTAGTACGGGAACTCTTGCCAATATGGTGAAACGAGGACGTGAATCGTTGGAATCAAATATGGACATCATCG
AAGACGCCTTACTGGAATCCAACATCCTGCATGTCGATGAAACGAGTTTGCGCATCAATGGGAAACTCGCATGGGTGCATGTCGCGTGTACATCGAGATA
TACATACTTGGCTCCTCACGCTTCTCGTGGAAAAAAAGCGACCGATGATATCGGGATTCTTCCCCGATATGAAGGGACGATGATGCACGATGCGTTCGGT
ACGTATCCGAAATACACACATGCCACCCATGCCCTTTGTCATGCCCACCATTTGCGTGAGTTAAAAGGATTCATCGAACAAGGGCATACGTGGGCGATGC
GCATGACCACGTTTCTGTTAGCCGCCAAGCAAGCCGTCGAAGCCCATCACGGTGCACTTTCCGAAGAAGAAGCGAGACGGTGGGAACGAGTGTATGATCG
CATCCTAGAAAGAGCACAACACCGATTAGAAACGATGACGCCTCTTCCGAAAAAAGCACTCGCTTTTGTTCGACGCCTTCAAAAACGAAAGGAAGAAGCG
CTGCGTTTCTTACGTGAAGTACATGTTCCCTTTGATAACAACCAAGCCGAACGCGATCTTCGCATGGTCAAAGTCAAAGAGAACATTTCGGGTACGTTTC
GCGAAGAAACATTCGCGCAGTCGTTTTGCATCGCAAGAAGCATCGTTTCCACACTGACGAAACACGAAAAAAACGTGTGGGATTCGTTATGTCTTCTGTT
GGCAGGCGAAACGATCGATCGAGTTCTTTCCGCTACCTAGGGCATTTTCTATGACCGAAATGCCCTATTTGTGCTGGGCTTTTTTATACACTAGCACTGG
GGTGAATAGTTAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1449 bp | 482 aa | 92 | 1540 | + | No |
Chemistry : DDE
ORF sequence :
MLTVQQAVFTVESLISKVQQQKQLITHQQQVIEQLLKENKQLRKENEQLKYRVQELEARTKKNSSNSHLPPSSDRFEKKRSSREPSGKKPGGQEGHEGKT
LRQVEHPHHRVVHRVHTCQGCGASLREVKPFKVDIRQVFDVPPAAIEVTQHEREVKSCPHCRCVQQAEFPSHVTNHVQYGPRLTALVVYLHHIQLIPYKR
LSDTIEALYQHSISTGTLANMVKRGRESLESNMDIIEDALLESNILHVDETSLRINGKLAWVHVACTSRYTYLAPHASRGKKATDDIGILPRYEGTMMHD
AFGTYPKYTHATHALCHAHHLRELKGFIEQGHTWAMRMTTFLLAAKQAVEAHHGALSEEEARRWERVYDRILERAQHRLETMTPLPKKALAFVRRLQKRK
EEALRFLREVHVPFDNNQAERDLRMVKVKENISGTFREETFAQSFCIARSIVSTLTKHEKNVWDSLCLLLAGETIDRVLSAT
LRQVEHPHHRVVHRVHTCQGCGASLREVKPFKVDIRQVFDVPPAAIEVTQHEREVKSCPHCRCVQQAEFPSHVTNHVQYGPRLTALVVYLHHIQLIPYKR
LSDTIEALYQHSISTGTLANMVKRGRESLESNMDIIEDALLESNILHVDETSLRINGKLAWVHVACTSRYTYLAPHASRGKKATDDIGILPRYEGTMMHD
AFGTYPKYTHATHALCHAHHLRELKGFIEQGHTWAMRMTTFLLAAKQAVEAHHGALSEEEARRWERVYDRILERAQHRLETMTPLPKKALAFVRRLQKRK
EEALRFLREVHVPFDNNQAERDLRMVKVKENISGTFREETFAQSFCIARSIVSTLTKHEKNVWDSLCLLLAGETIDRVLSAT
Blast result :
Comments
ISGst1 is 97% aa similar to ISBst12.
References
1] Stuknyte,M., Guglielmetti,S., Mora,D., Kuisiene,N., Parini,C. and Citavicius,D. (2008) Extremophiles In press.
2] Stuknyte,M., Guglielmetti,S., Ricci,G., Mora,D., Kuisiene,N., Parini,C. and Citavicius,D. (2007)Ann. Microbiol. 57 (3), 407-414.
3] Stuknyte,M. (2007) Direct Submission GenBank.
4] ISfinder annotation (2008).
2] Stuknyte,M., Guglielmetti,S., Ricci,G., Mora,D., Kuisiene,N., Parini,C. and Citavicius,D. (2007)Ann. Microbiol. 57 (3), 407-414.
3] Stuknyte,M. (2007) Direct Submission GenBank.
4] ISfinder annotation (2008).