ISAlg
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF133213 | ND | Vibrio cholerae | Vibrio cholerae serogroup O103 |
DNA section
IS Length : 1258 bp
Ends
IR Length : 25/36
IRL : TGTAGTGGTACGGTGAATTTGGTCACTTAATTAGAGGTGATATCATCATC
IRR : TGTAGTGGTCAACTAAAATTGGCCACGGTTTTAGAGTTTTCCCAATAGAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGTAGTGGTACGGTGAATTTGGTCACTTAATTAGAGGTGATATCATCATCTCATAAGTTAATAGGTGACATTTTGACAAAACGAACAAGACGGCTATTTA
GTGCCGAATTCAAACTGGAAGCGGCACAATTACTGCTCGATCAAAACTACTCAGTGACTGAAGCGGCTCAAGCCATGAATGTAGGTAAATCTACGATGGA
TAAGTGGGTTCGCCAATTAAAACTAGAGCGCCAAGGCAAAGCACCTAAAGCGTCACCAATGACGCCTGAGCAAATTGAAATTCGTGAATTAAAAAAGAAG
CTCGCTCGTCTTGAAGAGCATAATGAAATATTAAAAAAAGCCACGGCTCTCTTGATGTCGGACTCACTGAACAATTCTTGATGATCAAGAAACTCAAGCA
GAGCCACAGCATAAAAACATTATGCGAAGTCTTCAATGTTCATCGAAGCAGTTATAACTATTGGCTGAAACGGCCAACAGAAATAAAGGCGGAAACCGTA
AAACTTCGCAGTTTGATTAGCGAAGCTTATGCGGCAAGCAATGGTTCTGCGGGAGCAAGAACCATTGCCGATATCGTAACAAACCAAGGCGTGAGTCTGA
GTCGTTACCGAGCAGCAAAGCTGATGAAAACGCTGGGATTAGTAAGTTGCCAAGTCCCAAAACATCGTTACCGTAAAGCCTCACAAGAGCATATTGAAGT
CCCGAATCACTTAGACCGTCAGTTTGCTGTCACGGCACCAAATCAAGTCTGGGTGGGTGACGTGACCTATATTTGGACGGGAAATCGTTGGATGTATTTA
GCGGTAGTCATCAACCTTTTTGCTCGAAAAGTGATTGGTTGGGCAATGTCAGTATCACCTGATACACGATTAACAAGCAAAGCGCTTTCGATGGCTTATG
AGTCTCGTGGAAAGCCTAAAGGCGTCATGTTCCACAGTGATCAAGGCAGTCACTATACGAGTCGTCAATACCGTCAGTTACTGTGGCGTTTTCAAATAAA
ACAAAGTTTATCTCGCCGAGGAAATTGCTGGGATAATGCGCCTATGGAGCGCTTCTTTAGAAGCTTGAAAACGGAATGGGTTCCAACGGTAGGTTATCGT
AGCTTTGCAGAGGCTCAACAAGAGATCATCCGCTACATTATTGGATATTATAGCCAGCTCAGGCCCCATCAGTATAACGGTGGCTTGACACCCAATGAGT
CAGAACGATTCTATTGGGAAAACTCTAAAACCGTGGCCAATTTTAGTTGACCACTACA
GTGCCGAATTCAAACTGGAAGCGGCACAATTACTGCTCGATCAAAACTACTCAGTGACTGAAGCGGCTCAAGCCATGAATGTAGGTAAATCTACGATGGA
TAAGTGGGTTCGCCAATTAAAACTAGAGCGCCAAGGCAAAGCACCTAAAGCGTCACCAATGACGCCTGAGCAAATTGAAATTCGTGAATTAAAAAAGAAG
CTCGCTCGTCTTGAAGAGCATAATGAAATATTAAAAAAAGCCACGGCTCTCTTGATGTCGGACTCACTGAACAATTCTTGATGATCAAGAAACTCAAGCA
GAGCCACAGCATAAAAACATTATGCGAAGTCTTCAATGTTCATCGAAGCAGTTATAACTATTGGCTGAAACGGCCAACAGAAATAAAGGCGGAAACCGTA
AAACTTCGCAGTTTGATTAGCGAAGCTTATGCGGCAAGCAATGGTTCTGCGGGAGCAAGAACCATTGCCGATATCGTAACAAACCAAGGCGTGAGTCTGA
GTCGTTACCGAGCAGCAAAGCTGATGAAAACGCTGGGATTAGTAAGTTGCCAAGTCCCAAAACATCGTTACCGTAAAGCCTCACAAGAGCATATTGAAGT
CCCGAATCACTTAGACCGTCAGTTTGCTGTCACGGCACCAAATCAAGTCTGGGTGGGTGACGTGACCTATATTTGGACGGGAAATCGTTGGATGTATTTA
GCGGTAGTCATCAACCTTTTTGCTCGAAAAGTGATTGGTTGGGCAATGTCAGTATCACCTGATACACGATTAACAAGCAAAGCGCTTTCGATGGCTTATG
AGTCTCGTGGAAAGCCTAAAGGCGTCATGTTCCACAGTGATCAAGGCAGTCACTATACGAGTCGTCAATACCGTCAGTTACTGTGGCGTTTTCAAATAAA
ACAAAGTTTATCTCGCCGAGGAAATTGCTGGGATAATGCGCCTATGGAGCGCTTCTTTAGAAGCTTGAAAACGGAATGGGTTCCAACGGTAGGTTATCGT
AGCTTTGCAGAGGCTCAACAAGAGATCATCCGCTACATTATTGGATATTATAGCCAGCTCAGGCCCCATCAGTATAACGGTGGCTTGACACCCAATGAGT
CAGAACGATTCTATTGGGAAAACTCTAAAACCGTGGCCAATTTTAGTTGACCACTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 345 bp | 114 aa | 37 | 381 | + | No |
Description : First part of the transposase
ORF sequence :
VISSSHKLIGDILTKRTRRLFSAEFKLEAAQLLLDQNYSVTEAAQAMNVGKSTMDKWVRQLKLERQGKAPKASPMTPEQIEIRELKKKLARLEEHNEILK
KATALLMSDSLNNS
KATALLMSDSLNNS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 327 | 1250 | + | No |
Description : Second part of the transposase
ORF sequence :
NIKKSHGSLDVGLTEQFLMIKKLKQSHSIKTLCEVFNVHRSSYNYWLKRPTEIKAETVKLRSLISEAYAASNGSAGARTIADIVTNQGVSLSRYRAAKLM
KTLGLVSCQVPKHRYRKASQEHIEVPNHLDRQFAVTAPNQVWVGDVTYIWTGNRWMYLAVVINLFARKVIGWAMSVSPDTRLTSKALSMAYESRGKPKGV
MFHSDQGSHYTSRQYRQLLWRFQIKQSLSRRGNCWDNAPMERFFRSLKTEWVPTVGYRSFAEAQQEIIRYIIGYYSQLRPHQYNGGLTPNESERFYWENS
KTVANFS
KTLGLVSCQVPKHRYRKASQEHIEVPNHLDRQFAVTAPNQVWVGDVTYIWTGNRWMYLAVVINLFARKVIGWAMSVSPDTRLTSKALSMAYESRGKPKGV
MFHSDQGSHYTSRQYRQLLWRFQIKQSLSRRGNCWDNAPMERFFRSLKTEWVPTVGYRSFAEAQQEIIRYIIGYYSQLRPHQYNGGLTPNESERFYWENS
KTVANFS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1214 bp | 404 aa | 37 | 1250 | + | Yes |
Chemistry : DDE
ORF sequence :
VISSSHKLIGDILTKRTRRLFSAEFKLEAAQLLLDQNYSVTEAAQAMNVGKSTMDKWVRQLKLERQGKAPKASPMTPEQIEIRELKKKLARLEEHNEILK
KSHGSLDVGLTEQFLMIKKLKQSHSIKTLCEVFNVHRSSYNYWLKRPTEIKAETVKLRSLISEAYAASNGSAGARTIADIVTNQGVSLSRYRAAKLMKTL
GLVSCQVPKHRYRKASQEHIEVPNHLDRQFAVTAPNQVWVGDVTYIWTGNRWMYLAVVINLFARKVIGWAMSVSPDTRLTSKALSMAYESRGKPKGVMFH
SDQGSHYTSRQYRQLLWRFQIKQSLSRRGNCWDNAPMERFFRSLKTEWVPTVGYRSFAEAQQEIIRYIIGYYSQLRPHQYNGGLTPNESERFYWENSKTV
ANFS
KSHGSLDVGLTEQFLMIKKLKQSHSIKTLCEVFNVHRSSYNYWLKRPTEIKAETVKLRSLISEAYAASNGSAGARTIADIVTNQGVSLSRYRAAKLMKTL
GLVSCQVPKHRYRKASQEHIEVPNHLDRQFAVTAPNQVWVGDVTYIWTGNRWMYLAVVINLFARKVIGWAMSVSPDTRLTSKALSMAYESRGKPKGVMFH
SDQGSHYTSRQYRQLLWRFQIKQSLSRRGNCWDNAPMERFFRSLKTEWVPTVGYRSFAEAQQEIIRYIIGYYSQLRPHQYNGGLTPNESERFYWENSKTV
ANFS
Blast result :
Comments
ISAlg is 95% aa similar to ISVch4.The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Sozhamannan,S., Deng,Y.K., Li,M., Sulakvelidze,A., Kaper,J.B., Johnson,J.A., Nair,G.B. and Morris,J.G. Jr. (1999)Infect. Immun. 67 (10), 5033-5040.
2] Sozhamannan,S. and Morris,J.G. Jr. (1999) Direct Submission GenBank.
2] Sozhamannan,S. and Morris,J.G. Jr. (1999) Direct Submission GenBank.