ISSag4
- Family IS3
- Group IS150
Isoform ISSag2Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007432 | ND | Streptococcus agalactiae | Streptococcus agalactiae A909 Streptococcus agalactiae 2603V/R Streptococcus agalactiae NEM316 |
DNA section
IS Length : 1221 bp
Ends
IR Length : 12/15
IRL : TGAATAGGTTACACTAAACTAGACAGAAATTATAAAGTGTTCTACACTTA
IRR : TGAAAAGGCAACACTTTTCTGTACAACATTTATAAAGTGTTTTTCTAGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTATCAATA | CTTAATAAAA | 0 |
DNA sequence
TGAATAGGTTACACTAAACTAGACAGAAATTATAAAGTGTTCTACACTTAAAAAAAAACTGGAGAAAAACCATGTCTAGAAAAGTACGTCGTCACTTCAC
CGATGATTTTAAGCAACAAATTGTTGACCTTTACAATGTTGGTAGAAAGCGTAGCAGTCTCATCAAGGTATATGAGCTAACCCCTTCCACCTTCGATAAG
TGGGTTAGACAAGCCAAAACAACTGGTTCATTCAAGTCTGTTGATAATCTGACAGATGAACAGCGGGAGCTAATTGAACTCAGAAAACACAATAAAGAAC
TCGAAATGCAATTAGATATCCTAAAGCAAGCAGCGGTGATTATGGCACAAAAAGGGAAATAATCACTGCTAATAAGGATAAATACAGCATTTCAAAGATG
TGTCGCTGGCTGAATATGCCACACTCAAGTTATTACTATCAAGCCGTGGAGTCAGTATCTGAAACGGAGTTTGAAGAAACTATTAAAAGAATTTTCCTCG
ATAGCGAGTCCAGATACGGATCCAGAAAAATCAAAATATGCTTGAATAACGAAGGTATCACACTTTCACGTCGTCGGATTCGACGCATTATGAAGCGACT
CAATTTGGTTTCTGTTTATCAGAAAGCCACCTTCAAACCACATTCTAGAGGCAAGAATGAAGCCCCTATTCCCAACCACTTAGACAGGCAATTTAAGCAA
GAAAGACCACTACAGGCCTTAGTCACTGACTTAACCTATGTTCGTGCAGGCAATCGTTGGGCTTATGTTTGCCTCATCATTGACCTATACAACCGTGAAA
TCATCGGCCTGTCTCTTGGTTGGCACAAGACCGCTGAACTCGTTAAGCAAGCCATACAAAGCATCCCTTACGCCCTGACCAAAGTCAAGATGTTCCATTC
AGATCGTGGCAAAGAGTTTGATAATCAGTTAATTGATGAAATATTGGAAGCCTTTGGAATCACACGTTCGCTTAGTCAGGCTGGTTGTCCTTATGACAAT
GCCGTAGCTGAAAGTACGTATCGTGCTTTCAAAATTGAATTTGTTTATCAAGAAACCTTTCAATTGCTGGAAGAACTAGCTCTTAAGACTAAAGACTATG
TTCATTGGTGGAATTACCACCGCATTCATGGTAGTCTCAACTACCAAACACCAATGACTAAAAGATTAATTGCCTAGAAAAACACTTTATAAATGTTGTA
CAGAAAAGTGTTGCCTTTTCA
CGATGATTTTAAGCAACAAATTGTTGACCTTTACAATGTTGGTAGAAAGCGTAGCAGTCTCATCAAGGTATATGAGCTAACCCCTTCCACCTTCGATAAG
TGGGTTAGACAAGCCAAAACAACTGGTTCATTCAAGTCTGTTGATAATCTGACAGATGAACAGCGGGAGCTAATTGAACTCAGAAAACACAATAAAGAAC
TCGAAATGCAATTAGATATCCTAAAGCAAGCAGCGGTGATTATGGCACAAAAAGGGAAATAATCACTGCTAATAAGGATAAATACAGCATTTCAAAGATG
TGTCGCTGGCTGAATATGCCACACTCAAGTTATTACTATCAAGCCGTGGAGTCAGTATCTGAAACGGAGTTTGAAGAAACTATTAAAAGAATTTTCCTCG
ATAGCGAGTCCAGATACGGATCCAGAAAAATCAAAATATGCTTGAATAACGAAGGTATCACACTTTCACGTCGTCGGATTCGACGCATTATGAAGCGACT
CAATTTGGTTTCTGTTTATCAGAAAGCCACCTTCAAACCACATTCTAGAGGCAAGAATGAAGCCCCTATTCCCAACCACTTAGACAGGCAATTTAAGCAA
GAAAGACCACTACAGGCCTTAGTCACTGACTTAACCTATGTTCGTGCAGGCAATCGTTGGGCTTATGTTTGCCTCATCATTGACCTATACAACCGTGAAA
TCATCGGCCTGTCTCTTGGTTGGCACAAGACCGCTGAACTCGTTAAGCAAGCCATACAAAGCATCCCTTACGCCCTGACCAAAGTCAAGATGTTCCATTC
AGATCGTGGCAAAGAGTTTGATAATCAGTTAATTGATGAAATATTGGAAGCCTTTGGAATCACACGTTCGCTTAGTCAGGCTGGTTGTCCTTATGACAAT
GCCGTAGCTGAAAGTACGTATCGTGCTTTCAAAATTGAATTTGTTTATCAAGAAACCTTTCAATTGCTGGAAGAACTAGCTCTTAAGACTAAAGACTATG
TTCATTGGTGGAATTACCACCGCATTCATGGTAGTCTCAACTACCAAACACCAATGACTAAAAGATTAATTGCCTAGAAAAACACTTTATAAATGTTGTA
CAGAAAAGTGTTGCCTTTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 72 | 362 | + | No |
Description : First part of the transposase
ORF sequence :
MSRKVRRHFTDDFKQQIVDLYNVGRKRSSLIKVYELTPSTFDKWVRQAKTTGSFKSVDNLTDEQRELIELRKHNKELEMQLDILKQAAVIMAQKGK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 882 bp | 293 aa | 296 | 1177 | + | No |
Description : Second part of the transposase
ORF sequence :
RTRNAIRYPKASSGDYGTKREIITANKDKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNNEGITLSRRRIRRIMK
RLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALVTDLTYVRAGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMF
HSDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLEELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA
RLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALVTDLTYVRAGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMF
HSDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLEELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1106 bp | 368 aa | 72 | 1177 | + | Yes |
Chemistry : DDE
ORF sequence :
MSRKVRRHFTDDFKQQIVDLYNVGRKRSSLIKVYELTPSTFDKWVRQAKTTGSFKSVDNLTDEQRELIELRKHNKELEMQLDILKQAAVIMAQKREIITA
NKDKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNNEGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPI
PNHLDRQFKQERPLQALVTDLTYVRAGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFHSDRGKEFDNQLIDEILEAFGITRS
LSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLEELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA
NKDKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNNEGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPI
PNHLDRQFKQERPLQALVTDLTYVRAGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFHSDRGKEFDNQLIDEILEAFGITRS
LSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLEELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA
Blast result :
Comments
ISSag4 is 97% aa similar to ISSdy2.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISSag2 and ISSag4 are closely related.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISSag2 and ISSag4 are closely related.
References
1] Tettelin,H., Masignani,V., Cieslewicz,M.J., Donati,C., Medini,D., Ward,N.L., Angiuoli,S.V., Crabtree,J., Jones,A.L., Durkin,A.S., Deboy,R.T., Davidsen,T.M., Mora,M., Scarselli,M., Margarit Y Ros,I., Peterson,J.D., Hauser,C.R., Sundaram,J.P., Nelson,W.C., Madupu,R., Brinkac,L.M., Dodson,R.J., Rosovitz,M.J., Sullivan,S.A., Daugherty,S.C., Haft,D.H., Selengut,J., Gwinn,M.L., Zhou,L., Zafar,N., Khouri,H., Radune,D., Dimitrov,G., Watkins,K., O'connor,K.J., Smith,S., Utterback,T.R., White,O., Rubens,C.E., Grandi,G., Madoff,L.C., Kasper,D.L., Telford,J.L., Wessels,M.R., Rappuoli,R. and Fraser,C.M. (2005)(er) Proc. Natl. Acad. Sci. U.S.A. 102 (39), 13950-13955.