ISTth6
- Family IS630
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_005835 | ND | Thermus thermophilus | Thermus thermophilus HB8 Thermus thermophilus HB27 plasmid pTT27 Thermus thermophilus HB27 Thermus thermophilus HB8 plasmid pTT27 |
DNA section
IS Length : 1056 bp
Ends
IR Length : 18/26
IRL : AGTGCCAACCCCTTATCATCTCAACATGGCGGCCCCACTTCGGATCCAGC
IRR : AGTGCCCTCCCCCAAGATTTTCAACTCCACACCCCCCAGGGCTTTCAGGG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GGCGATGGACTC | TA | GCTCCCTCTCCC | 2 |
DNA sequence
AGTGCCAACCCCTTATCATCTCAACATGGCGGCCCCACTTCGGATCCAGCTGACCCCTGAGGAGGACCGGCTCCTCCTGGAGCTCTCCCTGGACCCAAGG
ACCCACAAGAAGACCCGCCTGTGGGCCATGATGGTCCGCCTGGCGGCCGAGGGCTGGACCGCCCCAAAGATTGCCCAGCACTTCCACAAGGACCGCACCA
CGGTCTACCTCGTCCTCAGGCGCTTCGTGAGGGAGGGTCTCCAGGGCCTCGCCTACCGCAAACCCCCGGGAGCCCCCAGGAAGTTCACCCCCGAGATGGC
CGCCTTCGTGGAGGAAAGGCTGGCCGAGGATAGGGTCTGGACCGCCCCGCAGCTTGCGGCAGCCCTAGCCGAGCGCTTCGGGGTCCGCCTGGCCCCCAAG
GTGGTGGCCCGGCACCTGAGGGCCATGGGGTACGTGTGGCGACGGACGCGGTACGTGCCTCTGGGAAAGCCCACGGAGGAAGAGGTGAAGGCTTTCGCGG
AGGAGGAAGGGGAAGCAAAAAGGGGGCGGAGGAGGGGGTGATGGAGGTAGGGTACCTGGATGAGAGCGGGTTTTCCCTGACCCTTCCCCCTACCTACGCC
TGGGGTCGGAGGGGGGAGGCCAAGGGGGTGCCCCGGGCCTGGGGTTCCCGGGGGCGGGTGAACGTGGTGGGGCACTTGGTGCGGGGACGGGAGGGGGAGC
GGCTTTTCTTTGCCCTTTTGGAGGGGCCGGTGCGGTGGGAGGTGGTGCGGGGGTATCTGGACCGGGTGGCCGAAGGTTTGGCCAAGCCCCTGAAGGTGTT
CATGGACAACGCGCCCTTCCACCGGTCCCGGGAGGTGGAGGGGAGGAAGGAGGCGTGGCGGGAACGGGGGCTTATGGTGGCTTACCTGCCGCGGTACAGC
CCGCATCTGAACCCCATGGAGAGCGTCTGGCGGCGGGTGAAGGGGTTTTTGATGCCGAGGCGGCACTACGGGAGCGTGGAGGAGCTTAAGGAGGCGGTGG
CGCAGGCCCTGAAAGCCCTGGGGGGTGTGGAGTTGAAAATCTTGGGGGAGGGCACT
ACCCACAAGAAGACCCGCCTGTGGGCCATGATGGTCCGCCTGGCGGCCGAGGGCTGGACCGCCCCAAAGATTGCCCAGCACTTCCACAAGGACCGCACCA
CGGTCTACCTCGTCCTCAGGCGCTTCGTGAGGGAGGGTCTCCAGGGCCTCGCCTACCGCAAACCCCCGGGAGCCCCCAGGAAGTTCACCCCCGAGATGGC
CGCCTTCGTGGAGGAAAGGCTGGCCGAGGATAGGGTCTGGACCGCCCCGCAGCTTGCGGCAGCCCTAGCCGAGCGCTTCGGGGTCCGCCTGGCCCCCAAG
GTGGTGGCCCGGCACCTGAGGGCCATGGGGTACGTGTGGCGACGGACGCGGTACGTGCCTCTGGGAAAGCCCACGGAGGAAGAGGTGAAGGCTTTCGCGG
AGGAGGAAGGGGAAGCAAAAAGGGGGCGGAGGAGGGGGTGATGGAGGTAGGGTACCTGGATGAGAGCGGGTTTTCCCTGACCCTTCCCCCTACCTACGCC
TGGGGTCGGAGGGGGGAGGCCAAGGGGGTGCCCCGGGCCTGGGGTTCCCGGGGGCGGGTGAACGTGGTGGGGCACTTGGTGCGGGGACGGGAGGGGGAGC
GGCTTTTCTTTGCCCTTTTGGAGGGGCCGGTGCGGTGGGAGGTGGTGCGGGGGTATCTGGACCGGGTGGCCGAAGGTTTGGCCAAGCCCCTGAAGGTGTT
CATGGACAACGCGCCCTTCCACCGGTCCCGGGAGGTGGAGGGGAGGAAGGAGGCGTGGCGGGAACGGGGGCTTATGGTGGCTTACCTGCCGCGGTACAGC
CCGCATCTGAACCCCATGGAGAGCGTCTGGCGGCGGGTGAAGGGGTTTTTGATGCCGAGGCGGCACTACGGGAGCGTGGAGGAGCTTAAGGAGGCGGTGG
CGCAGGCCCTGAAAGCCCTGGGGGGTGTGGAGTTGAAAATCTTGGGGGAGGGCACT
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
516 bp | 171 aa | 26 | 541 | + | No |
Description : First part of the transposase
ORF sequence :
MAAPLRIQLTPEEDRLLLELSLDPRTHKKTRLWAMMVRLAAEGWTAPKIAQHFHKDRTTVYLVLRRFVREGLQGLAYRKPPGAPRKFTPEMAAFVEERLA
EDRVWTAPQLAAALAERFGVRLAPKVVARHLRAMGYVWRRTRYVPLGKPTEEEVKAFAEEEGEAKRGRRRG
EDRVWTAPQLAAALAERFGVRLAPKVVARHLRAMGYVWRRTRYVPLGKPTEEEVKAFAEEEGEAKRGRRRG
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
666 bp | 241 aa | 394 | 1059 | + | No |
Description : Second part of the transposase
ORF sequence :
GLDRPAACGSPSRALRGPPGPQGGGPAPEGHGVRVATDAVRASGKAHGGRGEGFRGGGRGSKKGAEEGVMEVGYLDESGFSLTLPPTYAWGRRGEAKGVP
RAWGSRGRVNVVGHLVRGREGERLFFALLEGPVRWEVVRGYLDRVAEGLAKPLKVFMDNAPFHRSREVEGRKEAWRERGLMVAYLPRYSPHLNPMESVWR
RVKGFLMPRRHYGSVEELKEAVAQALKALGGVELKILGEGT
RAWGSRGRVNVVGHLVRGREGERLFFALLEGPVRWEVVRGYLDRVAEGLAKPLKVFMDNAPFHRSREVEGRKEAWRERGLMVAYLPRYSPHLNPMESVWR
RVKGFLMPRRHYGSVEELKEAVAQALKALGGVELKILGEGT
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1034 bp | 344 aa | 26 | 1059 | + | Yes |
Chemistry : DDE
ORF sequence :
MAAPLRIQLTPEEDRLLLELSLDPRTHKKTRLWAMMVRLAAEGWTAPKIAQHFHKDRTTVYLVLRRFVREGLQGLAYRKPPGAPRKFTPEMAAFVEERLA
EDRVWTAPQLAAALAERFGVRLAPKVVARHLRAMGYVWRRTRYVPLGKPTEEEVKAFAEEEGEAKKGAEEGVMEVGYLDESGFSLTLPPTYAWGRRGEAK
GVPRAWGSRGRVNVVGHLVRGREGERLFFALLEGPVRWEVVRGYLDRVAEGLAKPLKVFMDNAPFHRSREVEGRKEAWRERGLMVAYLPRYSPHLNPMES
VWRRVKGFLMPRRHYGSVEELKEAVAQALKALGGVELKILGEGT
EDRVWTAPQLAAALAERFGVRLAPKVVARHLRAMGYVWRRTRYVPLGKPTEEEVKAFAEEEGEAKKGAEEGVMEVGYLDESGFSLTLPPTYAWGRRGEAK
GVPRAWGSRGRVNVVGHLVRGREGERLFFALLEGPVRWEVVRGYLDRVAEGLAKPLKVFMDNAPFHRSREVEGRKEAWRERGLMVAYLPRYSPHLNPMES
VWRRVKGFLMPRRHYGSVEELKEAVAQALKALGGVELKILGEGT
Blast result :
Comments
ISTth6 is 44% aa similar to ISDge17.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The STOP codon of the transposase is in the target site.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The STOP codon of the transposase is in the target site.
References
1] Henne,A., Brueggemann,H., Raasch,C., Wiezer,A., Hartsch,T., Liesegang,H., Johann,A., Lienard,T., Gohl,O., Martinez-Arias,R., Jacobi,C., Starkuviene,V., Schlenczeck,S., Dencker,S., Huber,R., Klenk,H.-P., Overbeek,R., Kramer,W., Merkl,R., Gottschalk,G. and Fritz,H.-J. (2004) Nat. Biotechnol. 22 (5), 547-553