ISTth6

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_005835 ND Thermus thermophilus
Thermus thermophilus HB8
Thermus thermophilus HB27 plasmid pTT27
Thermus thermophilus HB27
Thermus thermophilus HB8 plasmid pTT27
DNA section
IS Length : 1056 bp

Ends


IR Length : 18/26

IRL : AGTGCCAACCCCTTATCATCTCAACATGGCGGCCCCACTTCGGATCCAGC
IRR : AGTGCCCTCCCCCAAGATTTTCAACTCCACACCCCCCAGGGCTTTCAGGG

Insertion site


Left flankDirect repeatRight flankDR Length
GGCGATGGACTCTAGCTCCCTCTCCC2

DNA sequence

AGTGCCAACCCCTTATCATCTCAACATGGCGGCCCCACTTCGGATCCAGCTGACCCCTGAGGAGGACCGGCTCCTCCTGGAGCTCTCCCTGGACCCAAGG
ACCCACAAGAAGACCCGCCTGTGGGCCATGATGGTCCGCCTGGCGGCCGAGGGCTGGACCGCCCCAAAGATTGCCCAGCACTTCCACAAGGACCGCACCA
CGGTCTACCTCGTCCTCAGGCGCTTCGTGAGGGAGGGTCTCCAGGGCCTCGCCTACCGCAAACCCCCGGGAGCCCCCAGGAAGTTCACCCCCGAGATGGC
CGCCTTCGTGGAGGAAAGGCTGGCCGAGGATAGGGTCTGGACCGCCCCGCAGCTTGCGGCAGCCCTAGCCGAGCGCTTCGGGGTCCGCCTGGCCCCCAAG
GTGGTGGCCCGGCACCTGAGGGCCATGGGGTACGTGTGGCGACGGACGCGGTACGTGCCTCTGGGAAAGCCCACGGAGGAAGAGGTGAAGGCTTTCGCGG
AGGAGGAAGGGGAAGCAAAAAGGGGGCGGAGGAGGGGGTGATGGAGGTAGGGTACCTGGATGAGAGCGGGTTTTCCCTGACCCTTCCCCCTACCTACGCC
TGGGGTCGGAGGGGGGAGGCCAAGGGGGTGCCCCGGGCCTGGGGTTCCCGGGGGCGGGTGAACGTGGTGGGGCACTTGGTGCGGGGACGGGAGGGGGAGC
GGCTTTTCTTTGCCCTTTTGGAGGGGCCGGTGCGGTGGGAGGTGGTGCGGGGGTATCTGGACCGGGTGGCCGAAGGTTTGGCCAAGCCCCTGAAGGTGTT
CATGGACAACGCGCCCTTCCACCGGTCCCGGGAGGTGGAGGGGAGGAAGGAGGCGTGGCGGGAACGGGGGCTTATGGTGGCTTACCTGCCGCGGTACAGC
CCGCATCTGAACCCCATGGAGAGCGTCTGGCGGCGGGTGAAGGGGTTTTTGATGCCGAGGCGGCACTACGGGAGCGTGGAGGAGCTTAAGGAGGCGGTGG
CGCAGGCCCTGAAAGCCCTGGGGGGTGTGGAGTTGAAAATCTTGGGGGAGGGCACT
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
516 bp171 aa26541+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAAPLRIQLTPEEDRLLLELSLDPRTHKKTRLWAMMVRLAAEGWTAPKIAQHFHKDRTTVYLVLRRFVREGLQGLAYRKPPGAPRKFTPEMAAFVEERLA
EDRVWTAPQLAAALAERFGVRLAPKVVARHLRAMGYVWRRTRYVPLGKPTEEEVKAFAEEEGEAKRGRRRG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
666 bp241 aa3941059+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GLDRPAACGSPSRALRGPPGPQGGGPAPEGHGVRVATDAVRASGKAHGGRGEGFRGGGRGSKKGAEEGVMEVGYLDESGFSLTLPPTYAWGRRGEAKGVP
RAWGSRGRVNVVGHLVRGREGERLFFALLEGPVRWEVVRGYLDRVAEGLAKPLKVFMDNAPFHRSREVEGRKEAWRERGLMVAYLPRYSPHLNPMESVWR
RVKGFLMPRRHYGSVEELKEAVAQALKALGGVELKILGEGT

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1034 bp344 aa261059+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAAPLRIQLTPEEDRLLLELSLDPRTHKKTRLWAMMVRLAAEGWTAPKIAQHFHKDRTTVYLVLRRFVREGLQGLAYRKPPGAPRKFTPEMAAFVEERLA
EDRVWTAPQLAAALAERFGVRLAPKVVARHLRAMGYVWRRTRYVPLGKPTEEEVKAFAEEEGEAKKGAEEGVMEVGYLDESGFSLTLPPTYAWGRRGEAK
GVPRAWGSRGRVNVVGHLVRGREGERLFFALLEGPVRWEVVRGYLDRVAEGLAKPLKVFMDNAPFHRSREVEGRKEAWRERGLMVAYLPRYSPHLNPMES
VWRRVKGFLMPRRHYGSVEELKEAVAQALKALGGVELKILGEGT

 

Blast result :
Comments
ISTth6 is 44% aa similar to ISDge17.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The STOP codon of the transposase is in the target site.
References
1] Henne,A., Brueggemann,H., Raasch,C., Wiezer,A., Hartsch,T., Liesegang,H., Johann,A., Lienard,T., Gohl,O., Martinez-Arias,R., Jacobi,C., Starkuviene,V., Schlenczeck,S., Dencker,S., Huber,R., Klenk,H.-P., Overbeek,R., Kramer,W., Merkl,R., Gottschalk,G. and Fritz,H.-J. (2004) Nat. Biotechnol. 22 (5), 547-553