ISDge19
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008025 | ND | Deinococcus geothermalis | Deinococcus geothermalis DSM 11300 Deinococcus geothermalis DSM 11300 plasmid pDGEO02 |
DNA section
IS Length : 1471 bp
Ends
Left end : CGAGTAATCCCGGAAGCCCCTGCCTTTAGGCATGGGGAGGAAAGGGATTTGACGCCGTAGGCGGCACTTGCCAGTTCATATATAGTCATGATAGGCTCCC II struct. : Yes
Right end : TGTGTCGGACGCTGAAGCCAAAGCTGAGCGCCTGAAAAGGTACTCAGAATTGCGGTGGAGTCCAGACGCAAGCCCCCGCCTTTAGGCGCGGGGTCATGAC II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| CTCCCGCCCGGAGAAAGC | TCAC | CCCCTCAGGAGAACCATGAACCTCATC | tgac |
DNA sequence
CGAGTAATCCCGGAAGCCCCTGCCTTTAGGCATGGGGAGGAAAGGGATTTGACGCCGTAGGCGGCACTTGCCAGTTCATATATAGTCATGATAGGCTCCC
TATGTGAAGATTACGCTCACCGCCAAACTGAAGTTGCGGCATACTCCAGAGCAGAAGAAAGCTCTGGACGCCGTAACTTTGGCGTACCGTGACGCGCTGA
ACTTCACCTCCCAGAAGGCGTTTGAAATGGGTAAAACCAGCAACGCCGCCAAGATCCAGAGAGAGGTGTACGCAACTCTGCGGGAAAGGTTCGGGCTGGG
CGCTCAAATGGCCTGCTCCGTGCCGCGTTACGTTGGAGCCGTCTACAAAACTCTCTGGGCGAAGGCCAAACAGAGTAAGGCAGCGCGTGAGGTCAACCCC
AAAGCCAAGCGGTATAAGGGACTGGACAACGCGCCCAAGTTCGTTTCTCGCACCCTGAGCTATCAGTACCAGCGCGATTACTCGTTCAAGAAAGGCCAGC
AGGTGAGTATTTCCACCCTGAATGGCCGTGTGGTGTTGCCCTATGAAGGCTACGCCAAACACCTTGAATACATCGCGCAGGGGGCTGAAATTGGGGCAGG
CAAACTCTGGTACAGCAAAGCCAAAAAGCAGTATTTCCTTTTGGTTCCGCTGACCTTTGAATTGCCCGACCCCGACCCCATGACCCATAAACAGGTGGTG
GGGGTGGATGTGGGAATGCGCTACTTTGCCACTGCATCGAACACCTCTGGGAAAGCTCTTTTCAAGTCGGGTAAGGCCACGCTGCGTAAAGCAGAGCGGT
TCCAAAAGGCCCGCAAATCGCTTCAGCAAAAAGGCACTCGTTCCGCCGTGCGCCGTTTGGTGCAACTATCGGGCCGAGAAAGACGGTTTATTGCTGAGAC
CAACCACGCCATCGCCGCTGAAATCCTTTCCCGCTTCCCAAATGCTTTTATCGGCGTCGAGGAGCTGACCGGAGTCCGTGACCGCACCGAACGGCGCAGC
CGGAAGAACAGTTCCGAGAAAACCCGGAAGGCAAACCGTAGACGTGCAAGGTGGAGTTACGCCGAGTTGCTGAGCTTCCTGGCGTACAAAGCACCGTTGC
ACGGTTCTATGGTGGTTAAAGTTGACGCCCATTACACCAGCCAAACCTGCCCCAGGTGTGGACACTGCTCAAGAGAGAACAGGCCGCACAAGGGGCTGAT
GTTTATCTGTGAAAGCTGTGGGTATCAGTTGCACGCTGACCTTGTAGGGGCAAGGAACATAGGACTCAGGGCATTGCTTGTCCGGCAAGACTGGGCAAGC
ACGGGGTGTTTGTCATGCACCCCTGGTCGCCCTCAAAGCGCAGACCCGCAAGCTGGGTCTGCTGGGCGAGATGTGTCGGACGCTGAAGCCAAAGCTGAGC
GCCTGAAAAGGTACTCAGAATTGCGGTGGAGTCCAGACGCAAGCCCCCGCCTTTAGGCGCGGGGTCATGAC
TATGTGAAGATTACGCTCACCGCCAAACTGAAGTTGCGGCATACTCCAGAGCAGAAGAAAGCTCTGGACGCCGTAACTTTGGCGTACCGTGACGCGCTGA
ACTTCACCTCCCAGAAGGCGTTTGAAATGGGTAAAACCAGCAACGCCGCCAAGATCCAGAGAGAGGTGTACGCAACTCTGCGGGAAAGGTTCGGGCTGGG
CGCTCAAATGGCCTGCTCCGTGCCGCGTTACGTTGGAGCCGTCTACAAAACTCTCTGGGCGAAGGCCAAACAGAGTAAGGCAGCGCGTGAGGTCAACCCC
AAAGCCAAGCGGTATAAGGGACTGGACAACGCGCCCAAGTTCGTTTCTCGCACCCTGAGCTATCAGTACCAGCGCGATTACTCGTTCAAGAAAGGCCAGC
AGGTGAGTATTTCCACCCTGAATGGCCGTGTGGTGTTGCCCTATGAAGGCTACGCCAAACACCTTGAATACATCGCGCAGGGGGCTGAAATTGGGGCAGG
CAAACTCTGGTACAGCAAAGCCAAAAAGCAGTATTTCCTTTTGGTTCCGCTGACCTTTGAATTGCCCGACCCCGACCCCATGACCCATAAACAGGTGGTG
GGGGTGGATGTGGGAATGCGCTACTTTGCCACTGCATCGAACACCTCTGGGAAAGCTCTTTTCAAGTCGGGTAAGGCCACGCTGCGTAAAGCAGAGCGGT
TCCAAAAGGCCCGCAAATCGCTTCAGCAAAAAGGCACTCGTTCCGCCGTGCGCCGTTTGGTGCAACTATCGGGCCGAGAAAGACGGTTTATTGCTGAGAC
CAACCACGCCATCGCCGCTGAAATCCTTTCCCGCTTCCCAAATGCTTTTATCGGCGTCGAGGAGCTGACCGGAGTCCGTGACCGCACCGAACGGCGCAGC
CGGAAGAACAGTTCCGAGAAAACCCGGAAGGCAAACCGTAGACGTGCAAGGTGGAGTTACGCCGAGTTGCTGAGCTTCCTGGCGTACAAAGCACCGTTGC
ACGGTTCTATGGTGGTTAAAGTTGACGCCCATTACACCAGCCAAACCTGCCCCAGGTGTGGACACTGCTCAAGAGAGAACAGGCCGCACAAGGGGCTGAT
GTTTATCTGTGAAAGCTGTGGGTATCAGTTGCACGCTGACCTTGTAGGGGCAAGGAACATAGGACTCAGGGCATTGCTTGTCCGGCAAGACTGGGCAAGC
ACGGGGTGTTTGTCATGCACCCCTGGTCGCCCTCAAAGCGCAGACCCGCAAGCTGGGTCTGCTGGGCGAGATGTGTCGGACGCTGAAGCCAAAGCTGAGC
GCCTGAAAAGGTACTCAGAATTGCGGTGGAGTCCAGACGCAAGCCCCCGCCTTTAGGCGCGGGGTCATGAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1353 bp | 450 aa | 104 | 1456 | + | No |
AG : TnpB
ORF sequence :
MKITLTAKLKLRHTPEQKKALDAVTLAYRDALNFTSQKAFEMGKTSNAAKIQREVYATLRERFGLGAQMACSVPRYVGAVYKTLWAKAKQSKAAREVNPK
AKRYKGLDNAPKFVSRTLSYQYQRDYSFKKGQQVSISTLNGRVVLPYEGYAKHLEYIAQGAEIGAGKLWYSKAKKQYFLLVPLTFELPDPDPMTHKQVVG
VDVGMRYFATASNTSGKALFKSGKATLRKAERFQKARKSLQQKGTRSAVRRLVQLSGRERRFIAETNHAIAAEILSRFPNAFIGVEELTGVRDRTERRSR
KNSSEKTRKANRRRARWSYAELLSFLAYKAPLHGSMVVKVDAHYTSQTCPRCGHCSRENRPHKGLMFICESCGYQLHADLVGARNIGLRALLVRQDWAST
GCLSCTPGRPQSADPQAGSAGRDVSDAEAKAERLKRYSELRWSPDASPRL
AKRYKGLDNAPKFVSRTLSYQYQRDYSFKKGQQVSISTLNGRVVLPYEGYAKHLEYIAQGAEIGAGKLWYSKAKKQYFLLVPLTFELPDPDPMTHKQVVG
VDVGMRYFATASNTSGKALFKSGKATLRKAERFQKARKSLQQKGTRSAVRRLVQLSGRERRFIAETNHAIAAEILSRFPNAFIGVEELTGVRDRTERRSR
KNSSEKTRKANRRRARWSYAELLSFLAYKAPLHGSMVVKVDAHYTSQTCPRCGHCSRENRPHKGLMFICESCGYQLHADLVGARNIGLRALLVRQDWAST
GCLSCTPGRPQSADPQAGSAGRDVSDAEAKAERLKRYSELRWSPDASPRL
Blast result :
Comments
ISDge19 is 46% aa similar to ISDge18. This IS had only the TnpB.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Saunders,E., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Daly,M.J., Fredrickson,J.K., Makarova,K.S., Gaidamakova,E.K., Zhai,M. and Richardson,P. US DOE Joint Genome Institute. (2006) Direct submission GenBank.